##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937623_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1661972 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.625158546594047 33.0 31.0 34.0 30.0 34.0 2 31.750218415232027 33.0 31.0 34.0 30.0 34.0 3 31.7589700668844 33.0 31.0 34.0 30.0 34.0 4 35.3699851742388 37.0 35.0 37.0 33.0 37.0 5 35.37206764012871 37.0 35.0 37.0 33.0 37.0 6 35.44069755687821 37.0 35.0 37.0 33.0 37.0 7 35.380305444375715 37.0 35.0 37.0 33.0 37.0 8 35.37275056378808 37.0 35.0 37.0 33.0 37.0 9 36.97797796834122 39.0 37.0 39.0 33.0 39.0 10-11 36.85147433290092 39.0 37.0 39.0 33.0 39.0 12-13 36.722314515527344 39.0 37.0 39.0 32.5 39.0 14-15 37.82538995843492 40.0 38.0 41.0 32.5 41.0 16-17 37.73309297629563 40.0 37.0 41.0 32.5 41.0 18-19 37.705479695205455 40.0 37.5 41.0 32.0 41.0 20-21 37.619350687015185 40.0 37.0 41.0 32.0 41.0 22-23 37.53202009420134 40.0 37.0 41.0 32.0 41.0 24-25 37.56875687436371 40.0 37.0 41.0 32.0 41.0 26-27 37.48467723884638 40.0 37.0 41.0 32.0 41.0 28-29 37.36627933563261 40.0 37.0 41.0 32.0 41.0 30-31 37.107869145809914 39.0 36.5 41.0 31.0 41.0 32-33 36.931136324799695 39.0 36.0 41.0 31.0 41.0 34-35 36.89892128146563 39.0 36.0 41.0 31.0 41.0 36-37 36.75422028770642 39.0 36.0 41.0 30.0 41.0 38-39 36.59296426173245 39.0 35.5 41.0 30.0 41.0 40-41 36.34360265997261 39.0 35.0 41.0 30.0 41.0 42-43 36.05622447309582 39.0 35.0 41.0 29.5 41.0 44-45 35.770779832632556 39.0 35.0 40.5 28.5 41.0 46-47 35.35599516718693 39.0 35.0 40.0 26.5 41.0 48-49 34.98245548059775 39.0 35.0 40.0 24.5 41.0 50-51 34.077947462412126 37.5 33.5 39.5 23.5 40.5 52-53 34.17181877913707 38.0 33.5 39.5 23.5 40.5 54-55 34.45935491091306 38.0 34.0 40.0 23.0 41.0 56-57 34.23283815852493 38.0 34.0 40.0 22.5 41.0 58-59 33.97526462539682 38.0 34.0 40.0 22.0 41.0 60-61 33.64990114153548 38.0 33.0 40.0 20.5 41.0 62-63 33.264211731605585 37.0 33.0 40.0 18.5 41.0 64-65 32.87051677164236 36.5 32.0 39.5 17.5 41.0 66-67 32.422453266360684 36.0 31.5 39.0 13.5 40.5 68-69 31.90830110254565 35.5 31.0 39.0 8.0 40.0 70-71 31.449943500853202 35.0 31.0 38.0 7.5 40.0 72-73 30.975511921981838 35.0 30.5 37.5 4.5 40.0 74-75 30.430253638448782 35.0 30.0 37.0 2.0 39.0 76-77 29.921872029131656 34.0 29.0 36.5 2.0 39.0 78-79 29.35184377354131 34.0 29.0 36.0 2.0 38.5 80-81 28.776831378627318 34.0 28.0 35.5 2.0 37.0 82-83 28.25693152471883 34.0 27.0 35.0 2.0 37.0 84-85 27.72692831166831 33.0 26.0 35.0 2.0 36.0 86-87 27.168841292151733 33.0 25.5 35.0 2.0 36.0 88-89 26.65649962815258 32.5 25.0 35.0 2.0 35.5 90-91 26.063415027449317 32.0 23.5 35.0 2.0 35.0 92-93 25.41667488982967 32.0 20.5 34.0 2.0 35.0 94-95 24.656983691662674 31.5 17.5 34.0 2.0 35.0 96-97 23.788735610467562 31.0 4.5 34.0 2.0 35.0 98-99 22.70485062323553 31.0 2.0 34.0 2.0 35.0 100-101 19.870388309791018 27.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.2838439541668052 1101 2 0.49012302576890576 1101 3 0.41917218260333655 1101 4 0.2484016458087055 1101 5 0.3560928498339635 1101 6 0.32585852427569506 1101 7 0.35187632173911254 1101 8 0.34135409570060915 1101 9 0.4131600001257709 1101 10-11 0.5342394361116973 1101 12-13 0.5891541986469306 1101 14-15 0.6874569425355403 1101 16-17 0.6870808106128266 1101 18-19 0.7274316695574399 1101 20-21 0.6251504654487476 1101 22-23 0.6402359202709036 1101 24-25 0.6234112105359984 1101 26-27 0.5566073847569655 1101 28-29 0.5540503825782892 1101 30-31 0.761976429729998 1101 32-33 0.7516209150188331 1101 34-35 0.6484389546123666 1101 36-37 0.813136876892024 1101 38-39 0.6875572500119276 1101 40-41 0.9147849350613484 1101 42-43 0.8433217638024857 1101 44-45 0.7334140467349428 1101 46-47 0.969949122421049 1101 48-49 1.0396809579839896 1101 50-51 1.0447581206234062 1101 52-53 0.9003421218328711 1101 54-55 0.9122918451427182 1101 56-57 0.9395420014421845 1101 58-59 0.760548692635119 1101 60-61 0.9446818689028547 1101 62-63 1.0044101541994124 1101 64-65 0.9078438995114553 1101 66-67 1.1120456282345188 1101 68-69 1.082237434067153 1101 70-71 1.2104031444865626 1101 72-73 1.0946276974990639 1101 74-75 1.0593379301239807 1101 76-77 1.1289276065217528 1101 78-79 1.3415785199726535 1101 80-81 1.349632389254218 1101 82-83 1.357198906182436 1101 84-85 1.478786000613038 1101 86-87 1.5574185300786745 1101 88-89 1.4872472320079915 1101 90-91 1.519824605885784 1101 92-93 1.634242050988103 1101 94-95 1.7132039066441322 1101 96-97 2.046129255745683 1101 98-99 2.033334759077537 1101 100-101 2.147939756094857 1102 1 0.27160206484003524 1102 2 0.39021351177423824 1102 3 0.30586101673539545 1102 4 0.1707255857077783 1102 5 0.09927070432581075 1102 6 0.19831486455913705 1102 7 0.2127678848169694 1102 8 0.16501062378850406 1102 9 0.2967343812864627 1102 10-11 0.2966258577799863 1102 12-13 0.4290833164828243 1102 14-15 0.3885966895426165 1102 16-17 0.457612067702577 1102 18-19 0.47826141659080434 1102 20-21 0.6005214974884936 1102 22-23 0.579046620569514 1102 24-25 0.6468576411565365 1102 26-27 0.5734605127434236 1102 28-29 0.594515277424442 1102 30-31 0.5304308008697802 1102 32-33 0.5384201631002696 1102 34-35 0.8113676353474304 1102 36-37 0.78123180047821 1102 38-39 0.8311385249273329 1102 40-41 0.9317950777279052 1102 42-43 0.7686224129716877 1102 44-45 0.8704388090668402 1102 46-47 0.7628539859781398 1102 48-49 0.5898969681380422 1102 50-51 0.698247525526817 1102 52-53 0.836733000353 1102 54-55 0.7432794477716342 1102 56-57 0.8254045923552198 1102 58-59 0.8382273805967984 1102 60-61 1.0359557944800883 1102 62-63 0.9530819019808447 1102 64-65 0.7883736849991791 1102 66-67 1.0119999034573866 1102 68-69 1.0172241589905724 1102 70-71 0.9322668583720883 1102 72-73 0.8498676518443631 1102 74-75 0.8207299254849332 1102 76-77 0.807525125996392 1102 78-79 0.9329742030981976 1102 80-81 0.9667710343116909 1102 82-83 1.3234587070081076 1102 84-85 1.1988638641272082 1102 86-87 1.290292323562106 1102 88-89 1.1032462614514174 1102 90-91 1.0834959200701881 1102 92-93 0.9761473201117035 1102 94-95 1.192600383383624 1102 96-97 1.5654435943175073 1102 98-99 1.6885022720613598 1102 100-101 2.1179536749973202 1103 1 0.3845565203680543 1103 2 0.4223574547967921 1103 3 0.31096191429677944 1103 4 0.3839170307607773 1103 5 0.3725046325625456 1103 6 0.2901514292522762 1103 7 0.26462985611999557 1103 8 0.28271444935754886 1103 9 0.27266890720328973 1103 10-11 0.3839312993760231 1103 12-13 0.47896336396403427 1103 14-15 0.6097277001842443 1103 16-17 0.6313347608032274 1103 18-19 0.6103751632327672 1103 20-21 0.6790608256710939 1103 22-23 0.5505589386102869 1103 24-25 0.6102388027800529 1103 26-27 0.5672612999318218 1103 28-29 0.6025810430588692 1103 30-31 0.4935508158638413 1103 32-33 0.573906116577021 1103 34-35 0.7137818880390725 1103 36-37 0.4195670427781337 1103 38-39 0.5659999339529307 1103 40-41 0.50223011385944 1103 42-43 0.5219290207727667 1103 44-45 0.49515256820384224 1103 46-47 0.48790552807605536 1103 48-49 0.41143261027968947 1103 50-51 0.29514854387372935 1103 52-53 0.5350963565245124 1103 54-55 0.5882891314385077 1103 56-57 0.47308426081978894 1103 58-59 0.6486359688639354 1103 60-61 0.6877496677385935 1103 62-63 0.700654737733359 1103 64-65 0.5811384239566735 1103 66-67 0.7616819043320362 1103 68-69 0.6472385283027222 1103 70-71 0.5748864464517993 1103 72-73 0.3522050599536506 1103 74-75 0.37376039259170213 1103 76-77 0.521217622716442 1103 78-79 0.6701717707276735 1103 80-81 0.8462476997982726 1103 82-83 0.8288459495579339 1103 84-85 0.7991812384998518 1103 86-87 0.8773611921349591 1103 88-89 1.0036590147365736 1103 90-91 0.9149570083893934 1103 92-93 0.8868008114380537 1103 94-95 0.9335864513983658 1103 96-97 1.256755158490023 1103 98-99 1.4020396218603963 1103 100-101 1.77077776546151 1104 1 0.31614693249659 1104 2 0.2991870430847605 1104 3 0.28972157193229364 1104 4 0.1507126741519329 1104 5 0.33232428728255314 1104 6 0.28611344428799157 1104 7 0.3528582657078658 1104 8 0.3141390937691355 1104 9 0.3748492941334618 1104 10-11 0.3650571037451229 1104 12-13 0.4655584617378352 1104 14-15 0.46219255784475166 1104 16-17 0.5150684912016033 1104 18-19 0.3565700027754488 1104 20-21 0.500456939709288 1104 22-23 0.4321776728613216 1104 24-25 0.49191897104677906 1104 26-27 0.4207813649061052 1104 28-29 0.4282789959525175 1104 30-31 0.5565767014507941 1104 32-33 0.5368062186199651 1104 34-35 0.4311223157864248 1104 36-37 0.5133170167467753 1104 38-39 0.5409513073676138 1104 40-41 0.5622017917369462 1104 42-43 0.5778541753990609 1104 44-45 0.5595931021591554 1104 46-47 0.6766693507295969 1104 48-49 0.5195289887965302 1104 50-51 0.44356708653392474 1104 52-53 0.49457677052730276 1104 54-55 0.6119043670744091 1104 56-57 0.6675608221809171 1104 58-59 0.5251221514166744 1104 60-61 0.6582927860875785 1104 62-63 0.7619908755121614 1104 64-65 0.7999940852574099 1104 66-67 0.9823033250196787 1104 68-69 0.9613816799718506 1104 70-71 0.9103172134398676 1104 72-73 0.8682666189198933 1104 74-75 0.9724407066340603 1104 76-77 0.8849944610512637 1104 78-79 0.9184487027754074 1104 80-81 0.998404346125767 1104 82-83 1.021973878086225 1104 84-85 0.9922789706475434 1104 86-87 1.206367210585988 1104 88-89 1.2010512969581946 1104 90-91 1.033786430076642 1104 92-93 0.9858309869935624 1104 94-95 1.0957637145650274 1104 96-97 1.1635714187203519 1104 98-99 1.1487992378457363 1104 100-101 1.0782506407816967 1105 1 0.3739526839404981 1105 2 0.4988573828380716 1105 3 0.4340495600131753 1105 4 0.4880836460283646 1105 5 0.44806087343921064 1105 6 0.4398276150291238 1105 7 0.5115066422381318 1105 8 0.49185485109278204 1105 9 0.5758741540216832 1105 10-11 0.46964605502478207 1105 12-13 0.4299000608533632 1105 14-15 0.608839729625636 1105 16-17 0.7255556535024965 1105 18-19 0.6771308531112581 1105 20-21 0.599067815946384 1105 22-23 0.6503543702597838 1105 24-25 0.7442547159361652 1105 26-27 0.7600917446366893 1105 28-29 0.7898873199148966 1105 30-31 0.7625662150291248 1105 32-33 0.7672694669748523 1105 34-35 0.7978825068840365 1105 36-37 0.9003474864550185 1105 38-39 0.9494965107739972 1105 40-41 0.9910596539404466 1105 42-43 0.8228001324321426 1105 44-45 0.8615965740427853 1105 46-47 1.1488309171671176 1105 48-49 0.9580326637347412 1105 50-51 1.0487456573012182 1105 52-53 0.98062340272201 1105 54-55 0.9359500619675103 1105 56-57 1.043740935504701 1105 58-59 1.1796335222675864 1105 60-61 1.2543178537621529 1105 62-63 1.18141708893301 1105 64-65 1.0988943399911264 1105 66-67 1.1481643566850863 1105 68-69 1.2836011179941238 1105 70-71 1.3176761830829022 1105 72-73 1.378841298363156 1105 74-75 1.1970489059246248 1105 76-77 1.0901684646334218 1105 78-79 1.116928694184164 1105 80-81 1.25662229631547 1105 82-83 1.2928688529893364 1105 84-85 1.1754977746128468 1105 86-87 1.370046897021986 1105 88-89 1.5259966450332492 1105 90-91 1.5444861927887423 1105 92-93 1.5684087803518878 1105 94-95 1.7276979668489325 1105 96-97 1.7956274626081168 1105 98-99 1.516518743552865 1105 100-101 1.3407368517914122 1106 1 0.23512537935171807 1106 2 0.2099168804405238 1106 3 0.24292708350318648 1106 4 0.4245547157443994 1106 5 0.1878649245782924 1106 6 0.3234495799658248 1106 7 0.285939968924275 1106 8 0.2685169429257215 1106 9 0.3313095387313112 1106 10-11 0.23014894785767126 1106 12-13 0.1804438925829004 1106 14-15 0.290265774375591 1106 16-17 0.28027444459942785 1106 18-19 0.3530690524514952 1106 20-21 0.43020887853298007 1106 22-23 0.5158457869072279 1106 24-25 0.3273309592547591 1106 26-27 0.43741412443651484 1106 28-29 0.5109032718778437 1106 30-31 0.5580014172667518 1106 32-33 0.59413612295387 1106 34-35 0.7859064784913912 1106 36-37 0.6640475494078615 1106 38-39 0.6308078588380326 1106 40-41 0.6006477755345685 1106 42-43 0.7619626641529393 1106 44-45 0.8318438627043676 1106 46-47 1.0324170393894718 1106 48-49 1.0447844144884115 1106 50-51 1.184873679656647 1106 52-53 1.203479715145356 1106 54-55 1.1255352118747695 1106 56-57 1.031549199140258 1106 58-59 0.9875582812416255 1106 60-61 1.0342507562008834 1106 62-63 1.124174319060181 1106 64-65 1.2086883942713413 1106 66-67 1.089653406410008 1106 68-69 0.8656724619395035 1106 70-71 0.9186679184046582 1106 72-73 1.197799096499594 1106 74-75 0.9371768448642825 1106 76-77 1.0182842717917993 1106 78-79 1.2630281596715385 1106 80-81 0.8911171942680056 1106 82-83 0.9112443047704595 1106 84-85 0.9545978227072531 1106 86-87 1.0963910313943686 1106 88-89 1.3936614335416877 1106 90-91 1.458271431064592 1106 92-93 1.5408652835888113 1106 94-95 1.728504102078933 1106 96-97 1.7040258667601407 1106 98-99 1.7528532199429456 1106 100-101 1.6513947806560054 1107 1 0.28706951434545047 1107 2 0.23890064430260693 1107 3 0.2787484005602856 1107 4 0.25621222092660645 1107 5 0.3222175275382071 1107 6 0.323762517446319 1107 7 0.3660868316170678 1107 8 0.3399556010777047 1107 9 0.3814388106765989 1107 10-11 0.42664600938344677 1107 12-13 0.3106121553178838 1107 14-15 0.30950205873551795 1107 16-17 0.3729270386680881 1107 18-19 0.37765055305208506 1107 20-21 0.42503516772207917 1107 22-23 0.4637406067894787 1107 24-25 0.3233998455983098 1107 26-27 0.425038711036521 1107 28-29 0.2483062307496553 1107 30-31 0.4307123536907511 1107 32-33 0.520228614720736 1107 34-35 0.5569759314348346 1107 36-37 0.6110236057679899 1107 38-39 0.51082379337047 1107 40-41 0.4283517209875569 1107 42-43 0.27557805963095205 1107 44-45 0.45170153281073055 1107 46-47 0.5604552351959313 1107 48-49 0.6219965076478999 1107 50-51 0.4964883673206302 1107 52-53 0.3859325086571772 1107 54-55 0.48463184243595236 1107 56-57 0.6083664157575015 1107 58-59 0.5533806268954535 1107 60-61 0.6153723886362101 1107 62-63 0.5624606943376023 1107 64-65 0.6657906803711597 1107 66-67 0.6105334842346863 1107 68-69 0.8917983831001806 1107 70-71 0.7141756965136778 1107 72-73 0.7302031624204446 1107 74-75 0.6592582888048852 1107 76-77 0.7307032262145015 1107 78-79 0.8321707807103529 1107 80-81 1.0696050960527046 1107 82-83 0.8501552474706457 1107 84-85 0.7454616609072424 1107 86-87 0.9535603506202364 1107 88-89 1.1522210145715732 1107 90-91 1.260906600007246 1107 92-93 0.8950691061332101 1107 94-95 1.1778472384621388 1107 96-97 1.5673578315368033 1107 98-99 1.8692844861455207 1107 100-101 1.9834652499924914 1108 1 0.19324895748248494 1108 2 0.2210521767709075 1108 3 0.32364953243440553 1108 4 0.19698892075516738 1108 5 0.4077384152516217 1108 6 0.41813888329544113 1108 7 0.38311187842853656 1108 8 0.3908726447338182 1108 9 0.31913879898402087 1108 10-11 0.4407300032374337 1108 12-13 0.4627319284490099 1108 14-15 0.47038179319880413 1108 16-17 0.6086620981108268 1108 18-19 0.6280394134872154 1108 20-21 0.5521917065883599 1108 22-23 0.5857281220845252 1108 24-25 0.5125776682433596 1108 26-27 0.43924601214335723 1108 28-29 0.5735930281865151 1108 30-31 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-1.3999516264831513 2316 62-63 -1.4154847922676908 2316 64-65 -1.3928081275347566 2316 66-67 -1.3983789825772774 2316 68-69 -1.362641947395005 2316 70-71 -1.5331735706445215 2316 72-73 -1.4955365755947057 2316 74-75 -1.5138917122057691 2316 76-77 -1.742001285218354 2316 78-79 -1.4902938791576013 2316 80-81 -1.364155713467877 2316 82-83 -1.5397044621035612 2316 84-85 -1.6420742915611832 2316 86-87 -1.5704881202628727 2316 88-89 -1.593806230235753 2316 90-91 -1.6543741354146881 2316 92-93 -1.998156372767344 2316 94-95 -2.1325506791185695 2316 96-97 -2.332129381333914 2316 98-99 -2.643342870903659 2316 100-101 -2.5567749107131093 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 6761.0 3 1422.0 4 1374.0 5 1488.0 6 1688.0 7 1974.0 8 2545.0 9 3087.0 10 3885.0 11 5059.0 12 6850.0 13 8809.0 14 10339.0 15 12964.0 16 16642.0 17 21845.0 18 18669.0 19 12769.0 20 12789.0 21 13035.0 22 14412.0 23 16060.0 24 17797.0 25 20051.0 26 23289.0 27 27254.0 28 32282.0 29 38167.0 30 46141.0 31 56618.0 32 71186.0 33 90713.0 34 120355.0 35 165202.0 36 230540.0 37 299706.0 38 208633.0 39 19570.0 40 2.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.94913377130114 19.53382682668485 14.874871370847279 21.64216803116673 2 14.922766155394424 20.44865738243574 41.41293107297184 23.215645389198 3 19.132633514673206 25.854416079378478 29.905090689041238 25.107859716907083 4 12.088609580627335 16.83777515524053 37.87645677519627 33.19715848893587 5 13.039537740790175 38.47405222621544 33.832332077439034 14.654077955555342 6 30.64376409482938 36.77764093623727 17.88458651851611 14.694008450417245 7 27.99932724356134 33.95032814961235 20.3963355270257 17.65400907980061 8 26.42892193209946 35.38398565743008 19.585758223527552 18.601334186942907 9 26.989740773538077 16.91666209535613 20.57716663542101 35.51643049568478 10-11 24.735954790311677 27.734641459476194 26.73368261040856 20.795721139803568 12-13 25.65488311083945 25.174872597988333 27.839765823830177 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21.103705808630604 27.349794017947303 30.613142772939405 20.933357400482688 40-41 21.254194536065853 27.100040532741865 30.65528937213427 20.990475559058012 42-43 21.183043968731926 27.108691727619238 30.378837721446274 21.32942658220256 44-45 21.15811212569111 27.44577207876913 30.12232627896154 21.273789516578216 46-47 21.341993821113707 27.570492050335314 29.590716600105495 21.496797528445484 48-49 21.460838471201814 27.7321625342692 29.269544596376928 21.537454398152057 50-51 21.518319668042388 27.991637764247283 28.99408729892009 21.49595526879024 52-53 21.639251250583726 28.093628949328785 28.89174751910658 21.37537228098091 54-55 21.455331555409824 28.03011238650518 28.97095117300458 21.54360488508042 56-57 21.376756020998574 28.45538254958323 28.774742648133454 21.39311878128474 58-59 21.48435157554001 28.01905552275974 29.004285955788028 21.49230694591222 60-61 21.59459579172501 28.029192945409818 29.10016943141493 21.276041831450243 62-63 21.44790666909747 28.04814804212125 29.27011027522853 21.23383501355275 64-65 21.45380550237115 27.871189480516428 29.169099890858973 21.50590512625345 66-67 21.29534543710127 28.22581325580751 28.883041104839457 21.59580020225177 68-69 21.36977021224275 28.137616500865047 28.969301662315573 21.52331162457663 70-71 21.350858377828477 28.21999037524781 28.88037373208053 21.54877751484318 72-73 21.42721115821167 28.24000472478717 28.64429343338954 21.688490683611615 74-75 21.30632583954434 28.415546234567657 28.655297659711486 21.622830266176514 76-77 21.478467258649047 28.28153336817001 28.658589138792806 21.581410234388137 78-79 21.445021867330162 28.257587264389766 28.583306812866766 21.71408405541331 80-81 21.280868336281426 28.62059405821252 28.47673518093855 21.621802424567505 82-83 21.490998108861593 28.48037567241481 28.360959141044695 21.6676670776789 84-85 21.41607554042026 28.314821655740047 28.31265220082162 21.956450603018073 86-87 21.39246414226008 28.311456970862753 28.472595716806786 21.823483170070375 88-89 21.599089034576128 28.233781112172107 28.367237908359265 21.799891944892497 90-91 21.359818394352217 28.340323149808082 28.578811649834755 21.721046806004942 92-93 21.50629097677143 28.145797232193193 28.506770969395273 21.841140821640103 94-95 21.357140597150302 28.072603014227404 28.493353361889596 22.0769030267327 96-97 21.52875500734944 28.03943926123554 28.356408171825535 22.075397559589494 98-99 21.334583294159053 28.002437242805794 28.35172113699426 22.311258326040893 100-101 21.69460734175194 27.47096846110625 28.846345618794693 21.98807857834712 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 2220.0 1 1310.5 2 469.5 3 559.0 4 496.0 5 473.0 6 813.5 7 1660.5 8 2840.5 9 3404.5 10 3014.5 11 2599.5 12 2662.0 13 2959.0 14 3168.0 15 3336.0 16 3502.0 17 3630.5 18 3774.0 19 3953.5 20 4371.0 21 4985.5 22 5794.5 23 6871.5 24 8183.5 25 9970.0 26 12232.5 27 14752.5 28 16720.0 29 18826.0 30 21917.5 31 24875.0 32 28267.5 33 32576.5 34 37219.0 35 41077.5 36 45674.5 37 50300.0 38 52669.5 39 53775.0 40 54606.0 41 56518.5 42 57826.0 43 59638.0 44 61855.5 45 62802.0 46 62012.5 47 61036.0 48 63593.0 49 63188.5 50 58865.5 51 55426.5 52 51568.5 53 48700.0 54 46082.0 55 41982.5 56 37594.0 57 32957.5 58 28927.5 59 25308.0 60 21695.0 61 18079.0 62 14217.5 63 10656.5 64 7762.5 65 5782.0 66 4287.0 67 3219.0 68 2345.0 69 1664.5 70 1257.5 71 943.0 72 739.5 73 566.0 74 440.0 75 350.5 76 226.5 77 149.5 78 96.5 79 55.5 80 36.5 81 28.0 82 21.5 83 15.0 84 11.0 85 9.5 86 8.5 87 7.0 88 4.5 89 1.5 90 2.5 91 3.0 92 1.5 93 1.0 94 0.5 95 0.5 96 1.5 97 1.5 98 1.0 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.30548047740876505 2 0.2730491247746653 3 0.27046183690218606 4 0.27341014168710426 5 0.283999971118647 6 0.1876686249828517 7 0.18802964189529064 8 0.1885711672639491 9 0.185021167624966 10-11 0.1932944718683588 12-13 0.18351693048980366 14-15 0.18607413361957964 16-17 0.19031608234073738 18-19 0.1850813371103725 20-21 0.17677794812427644 22-23 0.17674786338157322 24-25 0.17990676136541411 26-27 0.17704871080860568 28-29 0.18685633692986403 30-31 0.19004531965640817 32-33 0.1942571836348627 34-35 0.19377582775161076 36-37 0.17024955895767196 38-39 0.16399193247539667 40-41 0.16964786410360702 42-43 0.17831227000214203 44-45 0.18339659151899068 46-47 0.18634489630390885 48-49 0.18339659151899068 50-51 0.18565294722173417 52-53 0.20737413145347816 54-55 0.17572498212966284 56-57 0.16345040710673825 58-59 0.16339023762133176 60-61 0.15635040782877208 62-63 0.16333006813592527 64-65 0.15950930581261297 66-67 0.15968981426883244 68-69 0.1691063387349486 70-71 0.16296905122348632 72-73 0.15872710250232858 74-75 0.1656766780667785 76-77 0.16853472862358693 78-79 0.1730474400290739 80-81 0.16287879699537658 82-83 0.15968981426883244 84-85 0.15457540800928052 86-87 0.15237922179194355 88-89 0.15845633981799934 90-91 0.16681989828950186 92-93 0.15472583172279677 94-95 0.16092328871966555 96-97 0.16011100066667788 98-99 0.16366100030566097 100-101 0.1416690533895878 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1661972.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 55.78013867310408 #Duplication Level Percentage of deduplicated Percentage of total 1 77.0818718736558 42.996375022949614 2 13.064450201322941 14.574736878353123 3 4.214482173884039 7.052532002837304 4 1.8701087910829042 4.172597108015816 5 0.9842019982522675 2.7449461972428804 6 0.6131895773879251 2.052227979576033 7 0.37893430172357495 1.4795905528695807 8 0.28459226374989305 1.2699676749809314 9 0.21126004730685938 1.0605703261376829 >10 1.149445942714221 12.052565067198087 >50 0.09848338670713108 3.7966855769890353 >100 0.04670403507617686 4.444468362680739 >500 0.0016252908116337948 0.5549536333609858 >1k 3.25058162326759E-4 0.3236524402796862 >5k 2.1670544155117267E-4 0.8032382691535068 >10k+ 1.0835272077558633E-4 0.620892907375017 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10273 0.6181211235809027 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8030 0.4831609678141388 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5260 0.31649149323815323 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 2103 0.1265364278098548 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1853 0.11149405645823154 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 6.016948540649301E-5 0.0 0.0 0.0 0.0 7 6.016948540649301E-5 0.0 0.0 0.0 0.0 8 6.016948540649301E-5 0.0 0.0 0.0 0.0 9 6.016948540649301E-5 0.0 0.0 0.0 0.0 10-11 6.016948540649301E-5 0.0 0.0 0.0 0.0 12-13 6.016948540649301E-5 0.0 0.0 0.0 0.0 14-15 6.016948540649301E-5 0.0 0.0 0.0 0.0 16-17 9.025422810973952E-5 0.0 0.0 0.0 0.0 18-19 1.2033897081298602E-4 0.0 0.0 6.016948540649301E-5 0.0 20-21 1.2033897081298602E-4 0.0 0.0 1.2033897081298602E-4 0.0 22-23 1.2033897081298602E-4 0.0 0.0 2.4067794162597203E-4 0.0 24-25 1.8050845621947904E-4 0.0 0.0 3.610169124389581E-4 0.0 26-27 1.8050845621947904E-4 0.0 0.0 6.016948540649302E-4 0.0 28-29 1.8050845621947904E-4 0.0 0.0 0.001564406620568818 0.0 30-31 1.8050845621947904E-4 0.0 0.0 0.0032491522119506224 0.0 32-33 1.8050845621947904E-4 0.0 0.0 0.005716101113616836 0.0 34-35 1.8050845621947904E-4 0.0 0.0 0.007852117845547337 0.0 36-37 1.8050845621947904E-4 0.0 0.0 0.011041100572091466 0.0 38-39 1.8050845621947904E-4 0.0 0.0 0.014350422269448583 0.0 40-41 1.8050845621947904E-4 0.0 0.0 0.018261438820870626 0.0 42-43 2.4067794162597203E-4 0.0 0.0 0.023887285706377725 0.0 44-45 3.0084742703246503E-4 0.0 0.0 0.029723725790807544 0.0 46-47 3.0084742703246503E-4 0.0 0.0 0.03634236918552178 0.0 48-49 3.0084742703246503E-4 0.0 0.0 0.04356270743430094 0.0 50-51 3.0084742703246503E-4 0.0 0.0 0.05261821498797814 0.0 52-53 3.0084742703246503E-4 0.0 0.0 0.07259448414293382 0.0 54-55 3.0084742703246503E-4 0.0 0.0 0.09840719338231932 0.0 56-57 3.0084742703246503E-4 0.0 0.0 0.12247498754491651 0.0 58-59 3.0084742703246503E-4 0.0 0.0 0.16059235654992982 0.0 60-61 3.0084742703246503E-4 3.0084742703246504E-5 0.0 0.20472667409559248 0.0 62-63 3.0084742703246503E-4 6.016948540649301E-5 0.0 0.241038958538411 0.0 64-65 3.0084742703246503E-4 6.016948540649301E-5 0.0 0.2734101416871042 0.0 66-67 3.610169124389581E-4 6.016948540649301E-5 0.0 0.3475991171933101 0.0 68-69 3.610169124389581E-4 6.016948540649301E-5 0.0 0.4274440243277263 0.0 70-71 3.610169124389581E-4 6.016948540649301E-5 0.0 0.4703147826798526 0.0 72-73 3.610169124389581E-4 6.016948540649301E-5 0.0 0.5501897745569722 0.0 74-75 3.610169124389581E-4 6.016948540649301E-5 0.0 0.6608113734768095 0.0 76-77 3.610169124389581E-4 6.016948540649301E-5 0.0 0.7773596667091864 0.0 78-79 3.610169124389581E-4 6.016948540649301E-5 0.0 0.8960439766734939 0.0 80-81 5.415253686584371E-4 6.016948540649301E-5 0.0 1.0430681142642597 0.0 82-83 6.016948540649301E-4 6.016948540649301E-5 0.0 1.1998697932335802 0.0 84-85 6.317795967681766E-4 6.016948540649301E-5 0.0 1.3611841836083882 0.0 86-87 6.618643394714232E-4 6.016948540649301E-5 0.0 1.5696413657991832 0.0 88-89 6.618643394714232E-4 6.016948540649301E-5 0.0 1.8137790528360287 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5545 0.0 50.08671 1 GTATCAA 9715 0.0 39.308273 1 ATCAACG 11985 0.0 31.538094 3 TCAACGC 12110 0.0 31.094772 4 TATCAAC 12310 0.0 30.705446 2 CAACGCA 12315 0.0 30.57808 5 AACGCAG 12685 0.0 29.694973 6 GTACATG 10500 0.0 26.314676 1 ACGCAGA 14450 0.0 25.90429 7 CGCAGAG 14525 0.0 25.73783 8 TACATGG 10755 0.0 25.41931 2 GTACCGA 95 0.0012266748 25.000381 6 ACATGGG 10855 0.0 24.002531 3 GAGTACT 8100 0.0 23.545822 12-13 GCAGAGT 15355 0.0 23.511324 9 TACCTGG 1475 0.0 22.563807 2 GTACCTG 1560 0.0 22.55892 1 GTACTTT 8585 0.0 22.325615 14-15 CATGGGA 6865 0.0 21.816063 4 AGTACTT 8540 0.0 20.969921 12-13 >>END_MODULE