FastQCFastQC Report
Thu 26 May 2016
SRR937623_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937623_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1661972
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT137020.8244422890397671No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT114200.6871355233421502No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT79250.4768431718464571No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28020.16859489810899342No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22670.13640422341651964No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA62200.049.589791
GTATCAA106350.038.7453461
ATCAACG141800.029.0520153
TATCAAC142350.029.0399022
TCAACGC143900.028.6280444
CAACGCA146250.028.1680375
AACGCAG149450.027.649476
GTACATG100300.024.5452121
ACGCAGA169300.024.353737
CGCAGAG170600.024.0846218
TACATGG104100.023.5522482
GCAGAGT179250.022.6573779
GAGTACT106850.022.42793712-13
AGTACTT112100.022.41572412-13
ACATGGG103850.022.1905783
TGGTATC30350.022.0746482
TACCTGG12700.021.6998962
GTATAGG5150.021.2255291
GTACTTT112550.021.14247114-15
CATGGGG35600.020.8213064
Produced by FastQC (version 0.11.5)