FastQCFastQC Report
Thu 26 May 2016
SRR937622_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937622_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1725181
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT104650.6066030173065898No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT81330.4714287950075963No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55840.3236761823831818No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN22790.13210208088310732No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19040.11036523124240297No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA53250.048.4882351
GTATCAA93600.040.5399481
ATCAACG118700.031.5922363
TCAACGC120100.031.2653584
TATCAAC120600.031.213652
CAACGCA122250.030.7154985
AACGCAG124450.030.1194326
GTCTACG1153.8341113E-628.9438531
GTACATG104600.027.184771
GTGTACG1606.181472E-826.7472231
ACGCAGA143450.026.0322637
CGCAGAG143400.025.9088138
TACATGG107600.025.576022
GTACCTG14900.025.2114011
TACCTGG14050.025.033532
ACATGGG109650.024.143523
GCAGAGT151250.024.124369
GAGTACT82400.023.40795912-13
TGGTATC26900.022.9698282
GTACTTT87650.021.81490914-15
Produced by FastQC (version 0.11.5)