##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937622_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1725181 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.549030507523558 31.0 31.0 34.0 30.0 34.0 2 31.646495063416534 33.0 31.0 34.0 30.0 34.0 3 31.69251284357989 33.0 31.0 34.0 30.0 34.0 4 35.28757446320125 37.0 35.0 37.0 33.0 37.0 5 35.279002029352284 37.0 35.0 37.0 33.0 37.0 6 35.380300965521876 37.0 35.0 37.0 33.0 37.0 7 35.31536922792449 37.0 35.0 37.0 33.0 37.0 8 35.30889860252344 37.0 35.0 37.0 33.0 37.0 9 36.8835264241839 39.0 37.0 39.0 33.0 39.0 10-11 36.77232939616191 39.0 37.0 39.0 33.0 39.0 12-13 36.6453398223143 39.0 37.0 39.0 32.0 39.0 14-15 37.72161297858022 40.0 37.5 41.0 32.0 41.0 16-17 37.65620563871269 40.0 37.0 41.0 32.0 41.0 18-19 37.63994038886354 40.0 37.0 41.0 32.0 41.0 20-21 37.49590709612499 40.0 37.0 41.0 32.0 41.0 22-23 37.33620993971067 40.0 37.0 41.0 32.0 41.0 24-25 37.18437601619772 39.0 36.5 41.0 31.0 41.0 26-27 37.28378906329249 40.0 37.0 41.0 32.0 41.0 28-29 37.22769581858367 40.0 37.0 41.0 31.0 41.0 30-31 37.00427752218463 39.0 36.5 41.0 31.0 41.0 32-33 36.75688116203459 39.0 36.0 41.0 30.5 41.0 34-35 36.491399163334165 39.0 35.0 40.5 30.0 41.0 36-37 36.4767464979037 39.0 35.0 41.0 30.0 41.0 38-39 36.41892357961281 39.0 35.0 41.0 30.0 41.0 40-41 36.21389494783446 39.0 35.0 41.0 30.0 41.0 42-43 35.94446292881732 39.0 35.0 40.5 29.0 41.0 44-45 35.668681431107814 39.0 35.0 40.0 27.5 41.0 46-47 35.24826641378499 38.5 35.0 40.0 25.5 41.0 48-49 34.880830475179124 38.0 34.5 40.0 24.0 41.0 50-51 33.97973777823892 37.5 33.5 39.5 23.5 40.5 52-53 34.08272436341463 38.0 33.5 39.5 23.0 40.5 54-55 34.383060386127596 38.0 34.0 40.0 23.0 41.0 56-57 34.11508096831579 38.0 34.0 40.0 22.0 41.0 58-59 33.885171179140045 38.0 33.5 40.0 21.5 41.0 60-61 33.578284829244 38.0 33.0 40.0 19.5 41.0 62-63 33.204015694585095 37.0 33.0 40.0 18.5 41.0 64-65 32.83107569582554 36.5 32.0 39.5 17.0 41.0 66-67 32.41501326527477 36.0 31.5 39.0 13.0 41.0 68-69 31.931863671116247 35.5 31.0 39.0 8.0 40.0 70-71 31.488983474777427 35.0 31.0 38.5 7.5 40.0 72-73 31.00319937444245 35.0 30.5 37.5 4.5 40.0 74-75 30.497425777353214 35.0 30.0 37.0 2.0 39.0 76-77 30.010023875755643 34.0 29.0 36.5 2.0 39.0 78-79 29.464915565381254 34.0 29.0 36.0 2.0 38.5 80-81 28.924730796362816 34.0 28.5 35.5 2.0 37.5 82-83 28.394537732562554 34.0 27.5 35.0 2.0 37.0 84-85 27.895274466853042 33.5 26.5 35.0 2.0 36.5 86-87 27.35372491350183 33.0 26.0 35.0 2.0 36.0 88-89 26.83533177098519 33.0 25.0 35.0 2.0 35.5 90-91 26.292026170007667 32.5 24.5 35.0 2.0 35.0 92-93 25.704600560752755 32.0 22.0 34.5 2.0 35.0 94-95 25.03768387201111 32.0 19.0 34.0 2.0 35.0 96-97 24.21023707077692 31.0 8.5 34.0 2.0 35.0 98-99 23.22813084540115 31.0 2.0 34.0 2.0 35.0 100-101 20.707270135713294 28.0 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.47473099602394697 1101 2 0.5482970712457025 1101 3 0.44923859239081665 1101 4 0.4188139153715511 1101 5 0.34918534578898885 1101 6 0.4446835536070779 1101 7 0.32851088912472903 1101 8 0.4810679253247443 1101 9 0.6241510396305969 1101 10-11 0.41741012871852234 1101 12-13 0.5037190811773087 1101 14-15 0.7795008828503072 1101 16-17 0.7472673912778944 1101 18-19 0.7401671647535082 1101 20-21 0.7925122971395524 1101 22-23 0.7750930657280151 1101 24-25 0.6476552226920376 1101 26-27 0.7849700406497675 1101 28-29 0.7784884045778853 1101 30-31 0.7215310155549588 1101 32-33 0.8552736738991342 1101 34-35 0.8044249737729956 1101 36-37 0.6510683771623533 1101 38-39 0.6726664296874105 1101 40-41 0.8451223595425361 1101 42-43 0.8157369611963929 1101 44-45 0.868274223797421 1101 46-47 0.8854979948951112 1101 48-49 1.0197289652473955 1101 50-51 0.8399743756860829 1101 52-53 0.8193768083574398 1101 54-55 0.9449385871559599 1101 56-57 1.1121988536751601 1101 58-59 1.0760994978920664 1101 60-61 1.0744079251161693 1101 62-63 1.0991560861516163 1101 64-65 1.077097597476346 1101 66-67 1.1384529833130088 1101 68-69 1.1778133621574653 1101 70-71 1.2152174535747626 1101 72-73 0.9627193805045842 1101 74-75 0.8900988491065718 1101 76-77 1.128997897477138 1101 78-79 1.2170934102347353 1101 80-81 1.3992153779609033 1101 82-83 1.5851966291998885 1101 84-85 1.7652996977461761 1101 86-87 1.7081264586317921 1101 88-89 1.6274709414725628 1101 90-91 1.7314409758884892 1101 92-93 2.0301022657814336 1101 94-95 2.082892930550827 1101 96-97 2.3796654634160284 1101 98-99 2.871130695683174 1101 100-101 3.2603262046494983 1102 1 0.37319304013785626 1102 2 0.4410100137288957 1102 3 0.3877430639356838 1102 4 0.29845225077225024 1102 5 0.396646415811567 1102 6 0.3911050669008631 1102 7 0.3951986206927174 1102 8 0.47483620396951665 1102 9 0.43551484830843634 1102 10-11 0.3958810170120728 1102 12-13 0.43507450751256727 1102 14-15 0.7141380463394711 1102 16-17 0.7605804217410466 1102 18-19 0.7502658093002381 1102 20-21 0.6759850347056542 1102 22-23 0.7075826869023203 1102 24-25 0.7771391313268126 1102 26-27 0.6886244803086399 1102 28-29 0.8279455728470637 1102 30-31 0.7699163369877766 1102 32-33 0.7116821826311224 1102 34-35 0.7637985148970827 1102 36-37 0.7850578911519719 1102 38-39 0.9891169886834419 1102 40-41 1.115292804357047 1102 42-43 1.0268499923523038 1102 44-45 1.0630936435838336 1102 46-47 1.2288865472630093 1102 48-49 1.164068824979779 1102 50-51 0.9950981306984588 1102 52-53 1.0656974102542804 1102 54-55 1.2736082000864783 1102 56-57 1.4339147399847363 1102 58-59 1.4026806451645868 1102 60-61 1.3203201860818226 1102 62-63 1.509059181191141 1102 64-65 1.2873252485076492 1102 66-67 1.0467544659254244 1102 68-69 1.0202901712020847 1102 70-71 1.1643101169467336 1102 72-73 1.2923417247971862 1102 74-75 1.168196727003778 1102 76-77 1.1764918733900238 1102 78-79 1.3960281368791492 1102 80-81 1.535607214375137 1102 82-83 1.466704442520733 1102 84-85 1.6753736724967752 1102 86-87 1.6572962872786974 1102 88-89 1.6381114748327867 1102 90-91 1.8602340532588961 1102 92-93 1.9139098352138255 1102 94-95 2.0331126687738816 1102 96-97 2.211862356869066 1102 98-99 2.463051087290207 1102 100-101 2.3527682796987897 1103 1 0.22624634873624316 1103 2 0.27491771163747813 1103 3 0.25459423141080606 1103 4 0.21831724981528566 1103 5 0.152956279788107 1103 6 0.23571388660882064 1103 7 0.31291148484865516 1103 8 0.26631845410032184 1103 9 0.2436901892472818 1103 10-11 0.32900415575810626 1103 12-13 0.2495422024547551 1103 14-15 0.4298727142511254 1103 16-17 0.4761939243836508 1103 18-19 0.4697820696044417 1103 20-21 0.37382544637542026 1103 22-23 0.2742616011866019 1103 24-25 0.2842283295648045 1103 26-27 0.2692441423418117 1103 28-29 0.3979184220886296 1103 30-31 0.30172212647782004 1103 32-33 0.34518982915214735 1103 34-35 0.2830484363362942 1103 36-37 0.4788571508265633 1103 38-39 0.42718730349215406 1103 40-41 0.40358187210360796 1103 42-43 0.4723520266928247 1103 44-45 0.24153712494854318 1103 46-47 0.6144539097217034 1103 48-49 0.6490403988960267 1103 50-51 0.4112317263630274 1103 52-53 0.4947698461106569 1103 54-55 0.49230401982850935 1103 56-57 0.6951766491074167 1103 58-59 0.6830523959409263 1103 60-61 0.5985266929002222 1103 62-63 0.5127482609980447 1103 64-65 0.6503817145193906 1103 66-67 0.8071405018435271 1103 68-69 0.9325204473877307 1103 70-71 1.0502295515937057 1103 72-73 0.9130343182096112 1103 74-75 0.8709940529031464 1103 76-77 0.8260525135171903 1103 78-79 0.8180598780596497 1103 80-81 0.8653949185910506 1103 82-83 0.6882512978980877 1103 84-85 0.9143018449012814 1103 86-87 1.0329724812435082 1103 88-89 1.040161059331421 1103 90-91 1.2312183234975222 1103 92-93 1.4869784851705425 1103 94-95 1.3822293233989313 1103 96-97 1.3678700380354876 1103 98-99 1.4587479411506585 1103 100-101 1.7150234069373056 1104 1 0.289018692548467 1104 2 0.3762155514783707 1104 3 0.4408730954078912 1104 4 0.3974143278681481 1104 5 0.3746953553368684 1104 6 0.394059757655711 1104 7 0.36215801703758643 1104 8 0.4165472315097176 1104 9 0.5595694186455518 1104 10-11 0.5027022705679869 1104 12-13 0.4740212131172612 1104 14-15 0.7695276947358991 1104 16-17 0.6416251815634268 1104 18-19 0.6391685880908184 1104 20-21 0.7280293388620862 1104 22-23 0.6797249457321541 1104 24-25 0.7229496090198211 1104 26-27 0.7741731565045527 1104 28-29 0.6674637707403548 1104 30-31 0.7611022297862888 1104 32-33 0.632141081833268 1104 34-35 0.7396289284573356 1104 36-37 0.7638115502729548 1104 38-39 0.7288401012097765 1104 40-41 0.9275607513639912 1104 42-43 0.7527337469933642 1104 44-45 0.8175965760893291 1104 46-47 0.8573797128526266 1104 48-49 0.8711128571256381 1104 50-51 0.7175105674077642 1104 52-53 0.8251509352219486 1104 54-55 0.7929824908815988 1104 56-57 0.9386745392139275 1104 58-59 0.9897552813938475 1104 60-61 0.9245147048951097 1104 62-63 1.0300066962684369 1104 64-65 1.04526747500487 1104 66-67 1.0806537355866723 1104 68-69 1.180097541415421 1104 70-71 1.0328425832415356 1104 72-73 0.942062711396904 1104 74-75 0.9992040805492373 1104 76-77 1.098445673262006 1104 78-79 1.0664236634048123 1104 80-81 1.1622696137553383 1104 82-83 1.2569929705036742 1104 84-85 1.2269493853863338 1104 86-87 0.8361825949608566 1104 88-89 1.2043855974443503 1104 90-91 1.310386668553761 1104 92-93 1.396835480640899 1104 94-95 1.5752337956616103 1104 96-97 1.7699157797459648 1104 98-99 1.8838693876986845 1104 100-101 1.9060970139313405 1105 1 0.35276206114885866 1105 2 0.44044689951855887 1105 3 0.3607721740304122 1105 4 0.3514683526141269 1105 5 0.4460620471856842 1105 6 0.38104215304436195 1105 7 0.3842363756916356 1105 8 0.4499467131671082 1105 9 0.4303139065864343 1105 10-11 0.45349345994858226 1105 12-13 0.5529039932264936 1105 14-15 0.5392309188943827 1105 16-17 0.4833848956677187 1105 18-19 0.44085735841877494 1105 20-21 0.6225625232713199 1105 22-23 0.587900468924424 1105 24-25 0.49395953367820766 1105 26-27 0.6381720817448979 1105 28-29 0.43918638635834384 1105 30-31 0.5045214354521477 1105 32-33 0.46670882241616596 1105 34-35 0.5346739490789787 1105 36-37 0.6488923922001462 1105 38-39 0.6650654055342429 1105 40-41 0.6293559132586992 1105 42-43 0.5330466080830831 1105 44-45 0.3767830601432465 1105 46-47 0.5547330569576587 1105 48-49 0.44481763317922685 1105 50-51 0.48170745566529405 1105 52-53 0.5083944441917438 1105 54-55 0.5515850864502738 1105 56-57 0.6135705875443662 1105 58-59 0.6548622211844162 1105 60-61 0.7177993848709647 1105 62-63 0.5687227440446421 1105 64-65 0.5676633215166689 1105 66-67 0.7466547019064578 1105 68-69 0.7302311031954289 1105 70-71 0.629038994363615 1105 72-73 0.6308565346615929 1105 74-75 0.5425872600786796 1105 76-77 0.670941344703639 1105 78-79 0.6386265471825681 1105 80-81 0.6488224667482179 1105 82-83 0.7169678124525554 1105 84-85 1.042150366646716 1105 86-87 0.8816351687813011 1105 88-89 0.9439859121609651 1105 90-91 1.1947897612590133 1105 92-93 1.5027517192712843 1105 94-95 1.4640775012690348 1105 96-97 1.4975057758059798 1105 98-99 1.5251538941707388 1105 100-101 1.7259753684887684 1106 1 0.21294532881899286 1106 2 0.33436758174235237 1106 3 0.31369250268313564 1106 4 0.21422595439208436 1106 5 0.20166614861006593 1106 6 0.23061029124014 1106 7 0.2443316715127608 1106 8 0.288934237159431 1106 9 0.21615001797815836 1106 10-11 0.03501200559292528 1106 12-13 -0.02107864811775073 1106 14-15 0.058888838886296924 1106 16-17 0.08506688374732363 1106 18-19 0.07173189961822857 1106 20-21 0.23765306271975106 1106 22-23 0.02386790671275918 1106 24-25 0.2017974465987038 1106 26-27 0.18746073042994738 1106 28-29 0.49569356721836755 1106 30-31 0.5922291584544723 1106 32-33 0.5020389904466072 1106 34-35 0.37458159968341675 1106 36-37 0.5270865279714343 1106 38-39 0.30709385116847443 1106 40-41 0.3331117640180352 1106 42-43 0.36038543860468764 1106 44-45 0.11018689723116637 1106 46-47 0.2908406604192848 1106 48-49 0.46958086683919475 1106 50-51 0.48289654883969035 1106 52-53 0.5412123635101906 1106 54-55 0.4019144547366267 1106 56-57 0.3814149826011075 1106 58-59 0.28439424462112584 1106 60-61 0.21609340446890002 1106 62-63 0.3754006071284479 1106 64-65 0.6392991348984864 1106 66-67 0.7119376008524725 1106 68-69 0.719783709519298 1106 70-71 0.8006148269392916 1106 72-73 0.7148957473766799 1106 74-75 0.6955193744336654 1106 76-77 0.8727369806264633 1106 78-79 0.7976358747240724 1106 80-81 0.6375370254578527 1106 82-83 0.728816862861688 1106 84-85 0.584364226632573 1106 86-87 0.5850991129507435 1106 88-89 0.9060776217612627 1106 90-91 1.136384661417278 1106 92-93 0.9591915532612454 1106 94-95 0.9880776107076983 1106 96-97 0.9835296165979805 1106 98-99 1.0147229162941862 1106 100-101 0.9978908818402843 1107 1 0.36708828756721346 1107 2 0.5139728449002483 1107 3 0.5667152622848484 1107 4 0.4920531236666221 1107 5 0.3775907574294166 1107 6 0.38299293704383786 1107 7 0.5114013727929887 1107 8 0.3449975373016798 1107 9 0.5752894205386099 1107 10-11 0.5408530439997534 1107 12-13 0.5666702850259142 1107 14-15 0.8444951689005151 1107 16-17 0.8395476803333892 1107 18-19 0.8431846023209317 1107 20-21 0.8441164339857536 1107 22-23 0.8740457161010937 1107 24-25 0.8257839330851908 1107 26-27 0.7894877574569747 1107 28-29 0.7998809783136664 1107 30-31 0.7386107374018422 1107 32-33 0.8508298866059221 1107 34-35 0.9023020833249547 1107 36-37 0.9132618480283341 1107 38-39 0.8555557999593759 1107 40-41 0.7715008109597079 1107 42-43 0.6750813845506372 1107 44-45 0.7135208232624635 1107 46-47 0.9191301341034972 1107 48-49 1.106342957877601 1107 50-51 1.1547493226661558 1107 52-53 0.8923414531261145 1107 54-55 0.8969091204549784 1107 56-57 0.8638829769112064 1107 58-59 0.9493480141801527 1107 60-61 1.13442555240632 1107 62-63 1.2404966241981512 1107 64-65 1.145157954244148 1107 66-67 1.2012779280217458 1107 68-69 1.3378402529047833 1107 70-71 1.3486767046064365 1107 72-73 1.3051695738347213 1107 74-75 1.1868067144052148 1107 76-77 1.1682206221627354 1107 78-79 1.3006230724481114 1107 80-81 1.5470053753867283 1107 82-83 1.6941529226056424 1107 84-85 1.6492646533751056 1107 86-87 1.6765198099635477 1107 88-89 1.9355761992861567 1107 90-91 2.071368658572325 1107 92-93 2.185925194797523 1107 94-95 1.8749641683179377 1107 96-97 1.798536728973513 1107 98-99 1.988024836635578 1107 100-101 2.0428055333338833 1108 1 0.3694945648122001 1108 2 0.43814941535865515 1108 3 0.4195074432603896 1108 4 0.4163609289251937 1108 5 0.5775659788307834 1108 6 0.36173932349820603 1108 7 0.2901551010543528 1108 8 0.5015955274820172 1108 9 0.42862030660294437 1108 10-11 0.6078791533009067 1108 12-13 0.5373466489959995 1108 14-15 0.5600327268319703 1108 16-17 0.7060516036115061 1108 18-19 0.5875277400256991 1108 20-21 0.6631730117859647 1108 22-23 0.6581887895650524 1108 24-25 0.871181996683589 1108 26-27 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2316 60-61 -0.9908985071282714 2316 62-63 -1.0605722163443758 2316 64-65 -1.0204257771290557 2316 66-67 -1.0004641421413751 2316 68-69 -1.005670551132333 2316 70-71 -1.0169654241935895 2316 72-73 -0.9650430516410644 2316 74-75 -0.9735174853926658 2316 76-77 -1.1182613485297601 2316 78-79 -0.9125182985879974 2316 80-81 -0.8849214249611244 2316 82-83 -1.0719339092706655 2316 84-85 -0.9864599297178955 2316 86-87 -0.7403861595740011 2316 88-89 -0.9521433726439419 2316 90-91 -1.0402708346461154 2316 92-93 -1.1059363865377456 2316 94-95 -0.8525468355088535 2316 96-97 -0.9854547129126665 2316 98-99 -0.7296508020926886 2316 100-101 -0.5018933008386348 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 7093.0 3 1496.0 4 1452.0 5 1677.0 6 1916.0 7 2235.0 8 2712.0 9 3404.0 10 4306.0 11 5542.0 12 7242.0 13 9499.0 14 11051.0 15 13359.0 16 16973.0 17 22350.0 18 19062.0 19 13449.0 20 13139.0 21 13776.0 22 14912.0 23 16666.0 24 18513.0 25 20942.0 26 24252.0 27 28295.0 28 32957.0 29 39733.0 30 47738.0 31 58354.0 32 73755.0 33 93966.0 34 123382.0 35 170101.0 36 235047.0 37 306178.0 38 223380.0 39 25267.0 40 10.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.86712664985437 19.57409495322929 14.858298159117512 21.700480237798825 2 14.908283542184922 20.540069281380045 41.26392021016902 23.28772696626601 3 19.169272255994795 25.783013494821756 29.87987167714714 25.167842572036314 4 12.13368353326076 16.86783401267392 37.80659687853995 33.19188557552537 5 13.069317519353351 38.388482902965 33.84492790771071 14.697271669970933 6 30.677187398355095 36.81601691371219 17.817074020723943 14.689721667208772 7 27.997003171063177 33.96708134415909 20.354102054802475 17.68181342997526 8 26.458289533442596 35.39497725115951 19.59449335190255 18.55223986349534 9 26.96158865927106 16.929253455835756 20.601596256620816 35.50756162827236 10-11 24.742688101967538 27.725386054972972 26.694715406070703 20.837210436988787 12-13 25.64923674896063 25.176858642027653 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38-39 21.090121077143237 27.293034674638022 30.5912349357626 21.025609312456144 40-41 21.24029229799996 27.104949149786755 30.655289844763868 20.999468707449417 42-43 21.17633428795341 27.1359103913439 30.361157658074895 21.3265976626278 44-45 21.191776072606014 27.423312277759354 30.125073245405936 21.25983840422869 46-47 21.321218864749124 27.593362923593595 29.602349179395844 21.483069032261437 48-49 21.459570978181826 27.775218913575312 29.232890588516824 21.532319519726038 50-51 21.494197797602453 27.925640042746956 29.074377381284265 21.505784778366323 52-53 21.63259224318415 28.09896783368227 28.85886020211127 21.409579721022315 54-55 21.41677180684793 28.09166578344075 28.936223345330685 21.555339064380636 56-57 21.438985685394552 28.42392437233884 28.80180719498649 21.33528274728012 58-59 21.482854629438766 27.986046795204928 29.03901148831281 21.492087087043494 60-61 21.571962472486927 28.045772000443574 29.131692912585788 21.250572614483705 62-63 21.49459697830256 28.060933287734784 29.214609496614074 21.22986023734858 64-65 21.445807686657766 27.857733020647597 29.215407688515764 21.481051604178873 66-67 21.319052467203097 28.243321665826222 28.850763613518982 21.586862253451695 68-69 21.28036556988926 28.141507366122948 29.0185615556462 21.559565508341596 70-71 21.361402710124032 28.14699028979642 28.95476100751999 21.536845992559556 72-73 21.49113651888924 28.226168643764442 28.68538989533251 21.59730494201381 74-75 21.312925748799607 28.36891952826866 28.698731939656437 21.619422783275297 76-77 21.43429103322771 28.287974255391674 28.724725589627365 21.553009121753252 78-79 21.434301560391837 28.293951656103495 28.59187288539367 21.679873898110998 80-81 21.2855494645552 28.598785399947623 28.488781585088486 21.62688355040869 82-83 21.454328859381842 28.54748533621979 28.424410306604862 21.573775497793502 84-85 21.375179860396113 28.354851753234296 28.368988047429443 21.900980338940148 86-87 21.447620241928018 28.26460727990202 28.5333516792461 21.754420798923864 88-89 21.575286593879998 28.2556745443092 28.393419589696844 21.775619272113957 90-91 21.41026891628722 28.33218947815817 28.569118465074094 21.688423140480513 92-93 21.46919930710525 28.211569205722796 28.503758985960665 21.81547250121129 94-95 21.351060735289078 28.165179641918016 28.49870786480974 21.985051757983165 96-97 21.502676414284196 28.163548392903078 28.369178530390464 21.964596662422263 98-99 21.40256511057082 28.00493464699389 28.370300602342823 22.22219964009247 100-101 21.572167380074227 27.69336393075229 28.82892297956156 21.905545709611914 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 2396.0 1 1417.5 2 493.0 3 576.5 4 526.0 5 502.5 6 892.0 7 1848.0 8 3037.0 9 3611.0 10 3267.5 11 2899.5 12 2970.0 13 3094.5 14 3256.0 15 3437.5 16 3592.5 17 3755.5 18 3912.5 19 4142.5 20 4498.0 21 5137.5 22 6020.0 23 7088.0 24 8477.0 25 10208.0 26 12539.5 27 15325.5 28 17295.0 29 19551.5 30 22855.5 31 25884.5 32 29494.0 33 33822.5 34 38683.5 35 42722.0 36 47532.5 37 52217.5 38 54348.0 39 55700.5 40 56535.0 41 58328.0 42 59982.0 43 61869.0 44 63968.5 45 64817.5 46 64095.0 47 63380.5 48 66049.0 49 65684.5 50 61655.0 51 57660.5 52 53368.5 53 50863.5 54 48033.0 55 43604.5 56 38745.0 57 34044.0 58 30209.5 59 26507.0 60 22389.0 61 18344.5 62 14540.5 63 10971.5 64 8059.0 65 6027.0 66 4497.5 67 3457.5 68 2517.5 69 1779.5 70 1353.5 71 1036.0 72 803.5 73 643.5 74 463.0 75 352.0 76 250.5 77 138.5 78 87.5 79 63.0 80 41.5 81 33.0 82 22.5 83 12.0 84 12.5 85 8.0 86 6.0 87 7.5 88 6.5 89 5.5 90 4.5 91 4.0 92 2.5 93 3.5 94 3.5 95 1.0 96 1.0 97 1.5 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3134743542851446 2 0.2701745498008615 3 0.26205945926833185 4 0.26895728622098203 5 0.27115995365124007 6 0.20293522824561594 7 0.19482013771308634 8 0.19331304947133085 9 0.2045002814197467 10-11 0.20490603594637313 12-13 0.2009644205448588 14-15 0.20331200030605487 16-17 0.18742960883524684 18-19 0.19374778646414492 20-21 0.18328511617041923 22-23 0.19177697876338773 24-25 0.19183494369576293 26-27 0.19102343464250998 28-29 0.20238456138805144 30-31 0.19855887585128748 32-33 0.20513789567587398 34-35 0.19873277064841313 36-37 0.18447339728411105 38-39 0.1747352886450755 40-41 0.17192398942487774 42-43 0.18574862579636572 44-45 0.1872267315719336 46-47 0.18653115238343107 48-49 0.2027033685161151 50-51 0.198298033655599 52-53 0.21754239120416932 54-55 0.1897482061302553 56-57 0.1712284102363752 58-59 0.1736629373961341 60-61 0.16334517943334642 62-63 0.1683301636176146 64-65 0.16798237402336336 66-67 0.1667361279772963 68-69 0.17032995378455942 70-71 0.17227177901912902 72-73 0.16911269020467998 74-75 0.18209683505672739 76-77 0.17586560482639213 78-79 0.17861893911421467 80-81 0.17203991928962817 82-83 0.15386791298999933 84-85 0.15444756231375142 86-87 0.15804138812101456 88-89 0.16206995092109175 90-91 0.17012707652124617 92-93 0.1647073553441639 94-95 0.16450447808085064 96-97 0.16890981294136673 98-99 0.17806827225665017 100-101 0.16308433723765794 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1725181.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 55.70046506353455 #Duplication Level Percentage of deduplicated Percentage of total 1 77.04661680715668 42.91532387730565 2 13.041338232523097 14.528172092047805 3 4.197031823501929 7.013298733665358 4 1.916838662636184 4.270752198423963 5 1.0152530477470045 2.8275033458339505 6 0.5923239591071341 1.9795631994324843 7 0.3940448733069678 1.536393788936976 8 0.28428102059410026 1.2667668044662104 9 0.20924443138971888 1.0489510926325971 >10 1.1525667220309248 11.974384162984077 >50 0.10093433814912615 3.8659783102368777 >100 0.04743662273283867 4.532872530206574 >500 0.0014626213869990724 0.506968028619128 >1k 3.134188686426584E-4 0.32587476587088116 >5k 2.0894591242843891E-4 0.7982186006162282 >10k+ 1.0447295621421946E-4 0.6089784687212092 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10465 0.6066030173065898 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8133 0.4714287950075963 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5584 0.3236761823831818 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 2279 0.13210208088310732 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1904 0.11036523124240297 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 5.796493237521165E-5 0.0 0.0 0.0 0.0 8 5.796493237521165E-5 0.0 0.0 0.0 0.0 9 5.796493237521165E-5 0.0 0.0 0.0 0.0 10-11 5.796493237521165E-5 0.0 0.0 0.0 0.0 12-13 5.796493237521165E-5 0.0 0.0 0.0 0.0 14-15 5.796493237521165E-5 0.0 0.0 0.0 0.0 16-17 5.796493237521165E-5 0.0 0.0 0.0 0.0 18-19 5.796493237521165E-5 0.0 0.0 0.0 0.0 20-21 5.796493237521165E-5 0.0 0.0 2.8982466187605824E-5 0.0 22-23 5.796493237521165E-5 0.0 0.0 1.4491233093802912E-4 0.0 24-25 5.796493237521165E-5 0.0 0.0 5.216843913769047E-4 0.0 26-27 5.796493237521165E-5 0.0 0.0 8.11509053252963E-4 0.0 28-29 5.796493237521165E-5 0.0 0.0 0.001449123309380291 0.0 30-31 5.796493237521165E-5 0.0 0.0 0.0035358608748879104 0.0 32-33 8.694739856281747E-5 0.0 0.0 0.006434107493648492 0.0 34-35 1.159298647504233E-4 0.0 0.0 0.009013546984345411 0.0 36-37 1.159298647504233E-4 0.0 0.0 0.011621968941229934 0.0 38-39 1.159298647504233E-4 0.0 0.0 0.015157829816117845 0.0 40-41 1.159298647504233E-4 0.0 0.0 0.020548568527012527 0.0 42-43 1.159298647504233E-4 0.0 0.0 0.025823377373156785 0.0 44-45 1.159298647504233E-4 0.0 0.0 0.0323734147315557 0.0 46-47 1.159298647504233E-4 0.0 0.0 0.0393292066165811 0.0 48-49 1.159298647504233E-4 0.0 0.0 0.04692261275773382 0.0 50-51 1.159298647504233E-4 0.0 0.0 0.05567531754639078 0.0 52-53 1.159298647504233E-4 0.0 0.0 0.07738318472090755 0.0 54-55 1.159298647504233E-4 0.0 0.0 0.10567007172001083 0.0 56-57 1.159298647504233E-4 0.0 0.0 0.1334062918615496 0.0 58-59 1.159298647504233E-4 0.0 0.0 0.17687999114295833 0.0 60-61 1.159298647504233E-4 0.0 0.0 0.22725151737701726 0.0 62-63 1.159298647504233E-4 0.0 0.0 0.26513160068421804 0.0 64-65 1.159298647504233E-4 0.0 0.0 0.30025834970359633 0.0 66-67 1.159298647504233E-4 0.0 0.0 0.37648223577699963 0.0 68-69 1.7389479712563493E-4 0.0 0.0 0.4529669640460914 0.0 70-71 1.7389479712563493E-4 0.0 0.0 0.4967015055231886 0.0 72-73 1.7389479712563493E-4 0.0 0.0 0.5761134628772285 0.0 74-75 1.7389479712563493E-4 0.0 0.0 0.6868554661800703 0.0 76-77 1.7389479712563493E-4 0.0 0.0 0.80246652380243 0.0 78-79 1.7389479712563493E-4 0.0 0.0 0.9146576504146522 0.0 80-81 2.318597295008466E-4 0.0 0.0 1.0626131403023797 0.0 82-83 2.318597295008466E-4 0.0 0.0 1.2238715821702186 0.0 84-85 2.318597295008466E-4 0.0 0.0 1.3816521280955447 0.0 86-87 2.6084219568845243E-4 0.0 0.0 1.583543987558407 0.0 88-89 2.8982466187605824E-4 0.0 0.0 1.8239825270507848 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5325 0.0 48.488235 1 GTATCAA 9360 0.0 40.539948 1 ATCAACG 11870 0.0 31.592236 3 TCAACGC 12010 0.0 31.265358 4 TATCAAC 12060 0.0 31.21365 2 CAACGCA 12225 0.0 30.715498 5 AACGCAG 12445 0.0 30.119432 6 GTCTACG 115 3.8341113E-6 28.943853 1 GTACATG 10460 0.0 27.18477 1 GTGTACG 160 6.181472E-8 26.747223 1 ACGCAGA 14345 0.0 26.032263 7 CGCAGAG 14340 0.0 25.908813 8 TACATGG 10760 0.0 25.57602 2 GTACCTG 1490 0.0 25.211401 1 TACCTGG 1405 0.0 25.03353 2 ACATGGG 10965 0.0 24.14352 3 GCAGAGT 15125 0.0 24.12436 9 GAGTACT 8240 0.0 23.407959 12-13 TGGTATC 2690 0.0 22.969828 2 GTACTTT 8765 0.0 21.814909 14-15 >>END_MODULE