FastQCFastQC Report
Thu 26 May 2016
SRR937622_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937622_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1725181
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT139880.8108134740644604No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT119110.6904203095211459No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT83320.4829638165502634No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29760.17250363874862987No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22300.12926179919672198No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA62600.050.8610041
GTATCAA110450.039.109531
TATAACG953.4855257E-530.0079652
ATCAACG144050.029.6191083
TATCAAC145000.029.4905852
TCAACGC145150.029.361914
CAACGCA148450.028.6771975
AACGCAG152200.028.0241286
CGTACAC1301.000424E-525.5837153
GTACATG102150.024.9350851
ACGCAGA173400.024.4356547
CGCAGAG174100.024.0918488
TGGTATC30450.024.0293672
TACATGG105350.023.947982
ACATGGG106000.022.8598423
GAGTACT108250.022.62085212-13
GCAGAGT185750.022.2995389
TACCTGG14200.021.748732
GTGGTAT35800.021.503861
GTACCTG15500.021.4610421
Produced by FastQC (version 0.11.5)