FastQCFastQC Report
Thu 26 May 2016
SRR937621_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937621_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1693202
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT105830.6250287916031283No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80550.47572587322717547No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54050.3192176716068136No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN24120.14245199332389166No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19430.11475299462202385No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA55100.045.5547371
GTATCAA96400.039.4022451
ATCAACG121550.030.8865223
TCAACGC122900.030.5877294
TATCAAC124050.030.346972
CAACGCA125500.029.953155
AACGCAG129050.029.2284036
GTACATG105200.026.119411
TATAACG1501.054379E-625.3504472
ACGCAGA148650.025.1188777
CGCAGAG149900.024.8777248
TACATGG109400.024.8087982
ACATGGG109400.024.3692673
GAGTACT80400.023.39469712-13
GCAGAGT157700.023.19479
GTACTTT85100.021.85146314-15
AGTACTT85700.021.58763112-13
CATGGGG35500.021.5536164
CATGGGA72150.021.407684
GTACCTG15850.021.2951321
Produced by FastQC (version 0.11.5)