##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937621_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1693202 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.63219686723734 33.0 31.0 34.0 30.0 34.0 2 31.74718078528138 33.0 31.0 34.0 30.0 34.0 3 31.76127715417298 33.0 31.0 34.0 30.0 34.0 4 35.37496412123303 37.0 35.0 37.0 33.0 37.0 5 35.377008768002874 37.0 35.0 37.0 33.0 37.0 6 35.43777056724478 37.0 35.0 37.0 33.0 37.0 7 35.3785354612149 37.0 35.0 37.0 33.0 37.0 8 35.361934961097376 37.0 35.0 37.0 33.0 37.0 9 36.95716459111199 39.0 37.0 39.0 33.0 39.0 10-11 36.84564865857706 39.0 37.0 39.0 33.0 39.0 12-13 36.71938699576306 39.0 37.0 39.0 32.0 39.0 14-15 37.820079647909694 40.0 38.0 41.0 32.5 41.0 16-17 37.75209455221527 40.0 37.5 41.0 32.5 41.0 18-19 37.7172779739216 40.0 37.5 41.0 32.0 41.0 20-21 37.650157512216495 40.0 37.0 41.0 32.0 41.0 22-23 37.44623234557956 40.0 37.0 41.0 32.0 41.0 24-25 37.27470290018557 39.5 37.0 41.0 31.5 41.0 26-27 37.21860563594893 39.5 36.5 41.0 31.5 41.0 28-29 37.26760599148831 40.0 37.0 41.0 31.5 41.0 30-31 37.0757665062999 39.0 36.5 41.0 31.0 41.0 32-33 36.83215853749287 39.0 36.0 41.0 30.5 41.0 34-35 36.81780437301633 39.0 36.0 41.0 30.5 41.0 36-37 36.71458189867482 39.0 36.0 41.0 30.0 41.0 38-39 36.57944592553044 39.0 35.0 41.0 30.0 41.0 40-41 36.33009203863449 39.0 35.0 41.0 30.0 41.0 42-43 36.05672802181901 39.0 35.0 41.0 29.5 41.0 44-45 35.777176320368156 39.0 35.0 40.5 28.5 41.0 46-47 35.38261825818774 39.0 35.0 40.0 26.5 41.0 48-49 34.98650456354292 39.0 35.0 40.0 24.5 41.0 50-51 34.04509562355821 37.5 33.5 39.5 23.5 40.5 52-53 34.1477909900886 38.0 33.5 39.5 23.5 40.5 54-55 34.49413950609555 38.0 34.0 40.0 23.5 41.0 56-57 34.30235287933749 38.0 34.0 40.0 23.0 41.0 58-59 34.05652397055992 38.0 34.0 40.0 22.0 41.0 60-61 33.736392350115345 38.0 33.0 40.0 21.0 41.0 62-63 33.36564597726674 37.0 33.0 40.0 19.5 41.0 64-65 33.003503126029855 37.0 32.5 39.5 18.0 41.0 66-67 32.587796376333124 36.0 32.0 39.0 15.0 41.0 68-69 32.10231354557814 36.0 31.0 39.0 10.5 40.0 70-71 31.65479812804379 35.0 31.0 38.5 8.0 40.0 72-73 31.19137675244891 35.0 31.0 38.0 7.0 40.0 74-75 30.724810743195437 35.0 30.0 37.0 2.0 39.0 76-77 30.215875896673875 34.5 30.0 36.5 2.0 39.0 78-79 29.67812966202497 34.0 29.0 36.0 2.0 38.5 80-81 29.136122270113077 34.0 29.0 35.5 2.0 37.5 82-83 28.59882282208502 34.0 28.0 35.0 2.0 37.0 84-85 28.10898315735512 34.0 27.0 35.0 2.0 36.5 86-87 27.615358061235458 33.0 26.0 35.0 2.0 36.0 88-89 27.106108721818188 33.0 25.5 35.0 2.0 36.0 90-91 26.581334949994154 33.0 25.0 35.0 2.0 35.0 92-93 25.99265504056811 32.0 23.5 35.0 2.0 35.0 94-95 25.320395617297876 32.0 20.5 34.0 2.0 35.0 96-97 24.563748152907923 31.5 13.0 34.0 2.0 35.0 98-99 23.593214513094125 31.0 2.0 34.0 2.0 35.0 100-101 21.212617277796742 28.0 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.2510339868839644 1101 2 0.2327955816174665 1101 3 0.2039224555532151 1101 4 0.24159363377393106 1101 5 0.1818438783262195 1101 6 0.16409704004409775 1101 7 0.20262104821875226 1101 8 0.1469812191152613 1101 9 0.369905705345019 1101 10-11 0.23831645962123105 1101 12-13 0.41867395362304904 1101 14-15 0.6023900411696985 1101 16-17 0.6715114126185497 1101 18-19 0.5058528141154781 1101 20-21 0.670573798561442 1101 22-23 0.5939699846099558 1101 24-25 0.6465026323266088 1101 26-27 0.6521218369568516 1101 28-29 0.4391453266818388 1101 30-31 0.6964787814603355 1101 32-33 0.6805270822817633 1101 34-35 0.8312271121989241 1101 36-37 0.8073593471181937 1101 38-39 0.8473808933809721 1101 40-41 1.0822695858508382 1101 42-43 0.7724505002607174 1101 44-45 0.8460032357601861 1101 46-47 0.875503638395152 1101 48-49 1.066875141050879 1101 50-51 1.0416500087843659 1101 52-53 0.9853506906442533 1101 54-55 1.1703780061963727 1101 56-57 1.1603903064862848 1101 58-59 1.184591791390801 1101 60-61 1.1577540476101476 1101 62-63 1.3399624792863776 1101 64-65 1.0713731347386357 1101 66-67 1.2551605395391974 1101 68-69 1.1711150677016278 1101 70-71 1.1867766274084595 1101 72-73 1.2090925918060123 1101 74-75 1.0350340405305012 1101 76-77 1.1064793362724323 1101 78-79 1.250637205132552 1101 80-81 1.1267476284135114 1101 82-83 1.3081454313040908 1101 84-85 1.5649356129714356 1101 86-87 1.563730588887207 1101 88-89 1.6281540589400514 1101 90-91 1.8413299877478444 1101 92-93 1.9287634818276231 1101 94-95 2.186062847786811 1101 96-97 2.3773329492501887 1101 98-99 2.5411621663657336 1101 100-101 2.7376332919710684 1102 1 0.38088856344512934 1102 2 0.24798235093597043 1102 3 0.4103952243014355 1102 4 0.3439223097553068 1102 5 0.308417397953626 1102 6 0.3082848548905943 1102 7 0.2786480418505306 1102 8 0.3887814282208595 1102 9 0.4241419946711247 1102 10-11 0.36362584085875227 1102 12-13 0.48649735444045206 1102 14-15 0.5507209983030563 1102 16-17 0.6207149103849261 1102 18-19 0.6961360571532111 1102 20-21 0.7379039002627934 1102 22-23 0.7691263385687037 1102 24-25 0.7568486070438425 1102 26-27 0.7527719652716414 1102 28-29 0.5625946185742521 1102 30-31 0.766626105859153 1102 32-33 0.7148479643271912 1102 34-35 0.9014229110773542 1102 36-37 0.9718636718021543 1102 38-39 0.8716162322270904 1102 40-41 0.8089771231571774 1102 42-43 0.9744940322443725 1102 44-45 1.032655645226967 1102 46-47 1.1544058340039385 1102 48-49 1.2591135214329725 1102 50-51 1.1818087389430971 1102 52-53 1.2100346560467798 1102 54-55 1.2881690906940406 1102 56-57 1.387171030752036 1102 58-59 1.1126033872467076 1102 60-61 1.1392310935183332 1102 62-63 1.531766490311945 1102 64-65 1.427054153653188 1102 66-67 1.5462089189708195 1102 68-69 1.4744911726346217 1102 70-71 1.4577708294805056 1102 72-73 1.4547137304810391 1102 74-75 1.5531378329456764 1102 76-77 1.5896168707273333 1102 78-79 1.8104965340936765 1102 80-81 1.8370489305711004 1102 82-83 1.6491263266562441 1102 84-85 1.8845269065747097 1102 86-87 1.9391967993234793 1102 88-89 2.0103144999627673 1102 90-91 1.7714877674265814 1102 92-93 1.952114874280813 1102 94-95 2.2109159986279856 1102 96-97 2.4134302784014103 1102 98-99 2.605881299527976 1102 100-101 3.0897956339537735 1103 1 0.42348801800136116 1103 2 0.47113480839728794 1103 3 0.4488364112344989 1103 4 0.435584282838839 1103 5 0.2566321071491018 1103 6 0.30449268675162244 1103 7 0.3819629466943084 1103 8 0.34264992884337175 1103 9 0.42030017336325187 1103 10-11 0.44156315929119927 1103 12-13 0.5209985253659326 1103 14-15 0.6291111239922742 1103 16-17 0.4617013780436636 1103 18-19 0.5562286195531598 1103 20-21 0.5654773424872559 1103 22-23 0.5983828008915779 1103 24-25 0.6404864057039461 1103 26-27 0.5500603093755174 1103 28-29 0.5335178710791411 1103 30-31 0.5199284121376877 1103 32-33 0.6429909358099763 1103 34-35 0.6358018018107359 1103 36-37 0.8149226034438257 1103 38-39 0.927249273076086 1103 40-41 0.855642208827426 1103 42-43 1.0875131672417027 1103 44-45 1.0049765973820612 1103 46-47 1.135557268758184 1103 48-49 1.1461225479344321 1103 50-51 1.0818706201312196 1103 52-53 1.0831986004352316 1103 54-55 1.0855333967354213 1103 56-57 1.114791532323153 1103 58-59 1.2080868016061004 1103 60-61 1.3295898561910064 1103 62-63 1.3236018789406288 1103 64-65 1.3313847251833977 1103 66-67 1.3516859599383793 1103 68-69 1.405981286466364 1103 70-71 1.6633397658651567 1103 72-73 1.6482873505675997 1103 74-75 1.531725459672412 1103 76-77 1.5764013463305808 1103 78-79 1.8056597966774213 1103 80-81 1.7029483199112327 1103 82-83 1.8663037292767441 1103 84-85 1.930314168684152 1103 86-87 1.7971605747429358 1103 88-89 1.9253142035259394 1103 90-91 2.041915599880486 1103 92-93 2.1886147705279235 1103 94-95 2.435116360280915 1103 96-97 2.7302093440325237 1103 98-99 2.8363961361912864 1103 100-101 3.307751553797253 1104 1 0.3060462786075355 1104 2 0.3729977121828867 1104 3 0.27140857045806754 1104 4 0.32236695011130223 1104 5 0.22096858620809456 1104 6 0.14158345844829512 1104 7 0.16408634306836234 1104 8 0.20437051772826464 1104 9 0.29915029546914695 1104 10-11 0.3654802582190655 1104 12-13 0.3520066799576824 1104 14-15 0.5129111495493248 1104 16-17 0.6754259472023065 1104 18-19 0.5510432599560176 1104 20-21 0.5485373179398749 1104 22-23 0.5741144502993549 1104 24-25 0.6227082834844637 1104 26-27 0.6233337098513232 1104 28-29 0.6327821593626481 1104 30-31 0.7696002722491286 1104 32-33 0.7526555232056182 1104 34-35 0.680491733279986 1104 36-37 0.8684385907978225 1104 38-39 0.807447056238729 1104 40-41 0.8488348837354494 1104 42-43 0.9039021376396974 1104 44-45 0.874343397828838 1104 46-47 0.8609848366105695 1104 48-49 0.9288909837168191 1104 50-51 1.0213562214160063 1104 52-53 0.8922553007993415 1104 54-55 0.9560291640463774 1104 56-57 0.9943318525119551 1104 58-59 0.8851575141744945 1104 60-61 1.135561568323979 1104 62-63 1.0461623639566255 1104 64-65 1.0255684062188024 1104 66-67 0.9972067321398868 1104 68-69 0.9488861684937646 1104 70-71 0.9907257609178224 1104 72-73 0.8532432488820945 1104 74-75 1.2398651431436285 1104 76-77 1.396335174082381 1104 78-79 1.3806174776749636 1104 80-81 1.2719878481470381 1104 82-83 1.4036093300567103 1104 84-85 1.4450952536282102 1104 86-87 1.6434483344940567 1104 88-89 1.774390363531996 1104 90-91 1.9109272441137435 1104 92-93 1.8581185457142375 1104 94-95 2.244484044181238 1104 96-97 2.4772880313467596 1104 98-99 2.534577444522885 1104 100-101 2.674514532676401 1105 1 0.380362212615065 1105 2 0.2339890164799634 1105 3 0.3655469222136496 1105 4 0.2478867810742713 1105 5 0.2485308923513614 1105 6 0.24989437620114785 1105 7 0.3740616619615196 1105 8 0.3296730915913031 1105 9 0.38901395121585125 1105 10-11 0.4917413560677133 1105 12-13 0.4859680663847783 1105 14-15 0.6592845774886982 1105 16-17 0.6618675952049458 1105 18-19 0.7002691252224338 1105 20-21 0.8311514143908596 1105 22-23 0.8036873016653985 1105 24-25 0.6680490315708525 1105 26-27 0.6602148949779618 1105 28-29 0.7070268137000184 1105 30-31 0.7156057136807945 1105 32-33 0.7938689282854767 1105 34-35 0.8005779691756771 1105 36-37 0.7740405476123442 1105 38-39 0.8976114621726907 1105 40-41 0.8831289503073307 1105 42-43 0.873975514015946 1105 44-45 0.733121489209374 1105 46-47 0.6676466512575843 1105 48-49 0.6442820986017281 1105 50-51 0.5348172412321688 1105 52-53 0.5914040297911143 1105 54-55 0.6419919877384146 1105 56-57 0.701364488179884 1105 58-59 0.7002480456081983 1105 60-61 0.720897714494825 1105 62-63 0.6020368717204789 1105 64-65 0.6733013583934806 1105 66-67 0.6455509804332422 1105 68-69 0.8015763090981238 1105 70-71 0.944376884864834 1105 72-73 0.8323681012685711 1105 74-75 0.8688557104672796 1105 76-77 1.1633852920273071 1105 78-79 1.1646546664881434 1105 80-81 1.1715010205050334 1105 82-83 1.202413082118504 1105 84-85 1.071654809043153 1105 86-87 1.2024430993982769 1105 88-89 1.300102941392531 1105 90-91 1.415573761559962 1105 92-93 1.7503541054326348 1105 94-95 2.0108384558717702 1105 96-97 1.8721180043230135 1105 98-99 2.1401107477603567 1105 100-101 2.3648914854163117 1106 1 0.2318047147936575 1106 2 0.20254515992290223 1106 3 0.2686104015763142 1106 4 0.22359484479422065 1106 5 0.33265828946760223 1106 6 0.08069018047920196 1106 7 0.15910130426303937 1106 8 0.18123276532865873 1106 9 0.33543358043692706 1106 10-11 0.200014915570371 1106 12-13 0.17210243855016927 1106 14-15 0.23590675757470336 1106 16-17 0.24924572341826234 1106 18-19 0.40982733943877037 1106 20-21 0.44585190071965286 1106 22-23 0.37508866459784684 1106 24-25 0.3321671797077812 1106 26-27 0.3258801086578984 1106 28-29 0.4077911464993207 1106 30-31 0.5631744261123899 1106 32-33 0.5231859205673004 1106 34-35 0.42302915363311655 1106 36-37 0.5024849904727233 1106 38-39 0.5770911135271604 1106 40-41 0.4582098566003765 1106 42-43 0.5310217125785286 1106 44-45 0.610118974698814 1106 46-47 0.7822974621916998 1106 48-49 0.888719568195036 1106 50-51 0.6967951149648925 1106 52-53 0.7197815111104902 1106 54-55 0.6609517270561938 1106 56-57 0.8076551306720887 1106 58-59 0.9206925223280393 1106 60-61 0.853457953150567 1106 62-63 0.9225527651736627 1106 64-65 0.8355267180611605 1106 66-67 0.9285189582927131 1106 68-69 0.7232319744098206 1106 70-71 1.1068938368717163 1106 72-73 1.0780690634119097 1106 74-75 1.2039895355350474 1106 76-77 1.1682831077356042 1106 78-79 1.3806869434656655 1106 80-81 1.2521295323103985 1106 82-83 0.9223331826989281 1106 84-85 1.1434257301809012 1106 86-87 1.379211320689464 1106 88-89 1.4865883827149737 1106 90-91 1.5733004907537662 1106 92-93 1.67693743624136 1106 94-95 1.7394149951852818 1106 96-97 2.105450461468518 1106 98-99 2.4459688356274434 1106 100-101 2.738533988307733 1107 1 0.13362014674574496 1107 2 0.2092340042175671 1107 3 0.2032374202405549 1107 4 0.14324155795527815 1107 5 0.1374503568607679 1107 6 0.16374744975179567 1107 7 0.13664992478104665 1107 8 0.2284765764349288 1107 9 0.3325557210058534 1107 10-11 0.2773658973466766 1107 12-13 0.23573274743549888 1107 14-15 0.5134265864901408 1107 16-17 0.5297299166550076 1107 18-19 0.4790774309301611 1107 20-21 0.6494724881030294 1107 22-23 0.6084391832802964 1107 24-25 0.6419054189451785 1107 26-27 0.5885026680926089 1107 28-29 0.49349125941097327 1107 30-31 0.6453341258471923 1107 32-33 0.6704144252944957 1107 34-35 0.6651953661671755 1107 36-37 0.7152029004105032 1107 38-39 0.713005255580768 1107 40-41 0.7645275754035481 1107 42-43 0.8456512721884124 1107 44-45 0.8047198554443398 1107 46-47 0.5745148838863443 1107 48-49 0.5903221417480395 1107 50-51 0.7348004639590116 1107 52-53 0.8727613986151113 1107 54-55 0.7100286179793827 1107 56-57 0.8793397169315611 1107 58-59 0.7397318801018571 1107 60-61 0.9612465152528387 1107 62-63 0.9809671977449455 1107 64-65 1.1298846479796225 1107 66-67 0.9200323732716313 1107 68-69 0.9239573580232374 1107 70-71 1.141007983434843 1107 72-73 1.292658210712176 1107 74-75 1.2073786193550298 1107 76-77 1.3518787886483317 1107 78-79 1.5100002637444945 1107 80-81 1.308959342719433 1107 82-83 1.369160859754274 1107 84-85 1.497768405753309 1107 86-87 1.53957491006571 1107 88-89 1.4765286661561596 1107 90-91 1.5947558715747476 1107 92-93 1.6003773563186456 1107 94-95 1.7767324243379896 1107 96-97 1.8103181068184142 1107 98-99 2.240788124960499 1107 100-101 2.6219815485663034 1108 1 0.2820401659856877 1108 2 0.22175681620813847 1108 3 0.3269080991327904 1108 4 0.06159219915661396 1108 5 0.20164733575394678 1108 6 0.24797706504742933 1108 7 0.2241419475188593 1108 8 0.16776226577106002 1108 9 0.269583326339621 1108 10-11 0.2171759388551422 1108 12-13 0.1116344913995917 1108 14-15 0.29302895823857966 1108 16-17 0.31415069858901745 1108 18-19 0.3813099959122255 1108 20-21 0.4322096721921511 1108 22-23 0.4722800054119034 1108 24-25 0.45480189349868994 1108 26-27 0.4146156117424269 1108 28-29 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-1.2953256378581628 2316 54-55 -1.3798152305186093 2316 56-57 -1.3918940275371838 2316 58-59 -1.379769837318662 2316 60-61 -1.3625841166410595 2316 62-63 -1.4334812750269421 2316 64-65 -1.3084198259238882 2316 66-67 -1.5111748641875096 2316 68-69 -1.42399880393398 2316 70-71 -1.4282889350484105 2316 72-73 -1.3783332190980602 2316 74-75 -1.3778162010153885 2316 76-77 -1.4879237003045205 2316 78-79 -1.5288175912787665 2316 80-81 -1.4579176579909046 2316 82-83 -1.4609781021672674 2316 84-85 -1.5125109018663814 2316 86-87 -1.696469549139021 2316 88-89 -1.6512869348487698 2316 90-91 -1.746516803142427 2316 92-93 -1.6555567390143366 2316 94-95 -1.6449447436555644 2316 96-97 -1.4536746421921833 2316 98-99 -1.2407978060290255 2316 100-101 -1.030482650057916 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 7141.0 3 1374.0 4 1297.0 5 1604.0 6 1796.0 7 1994.0 8 2536.0 9 3178.0 10 4055.0 11 4992.0 12 6840.0 13 8961.0 14 10043.0 15 12669.0 16 16498.0 17 21827.0 18 18567.0 19 12791.0 20 12301.0 21 12873.0 22 14020.0 23 15533.0 24 17103.0 25 19617.0 26 22869.0 27 26275.0 28 31354.0 29 37461.0 30 45066.0 31 54997.0 32 69777.0 33 90352.0 34 120194.0 35 165677.0 36 232144.0 37 308267.0 38 231789.0 39 27364.0 40 6.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.95399022487758 19.56481690452877 14.809411176527384 21.671781694066272 2 14.880595962988036 20.44755266292235 41.40984478452429 23.262006589565317 3 19.1795083161302 25.89826405297499 29.77486104251572 25.147366588379093 4 12.089535535740614 16.850115078375865 37.80491920828919 33.25543017759433 5 12.976705107610762 38.47242394655491 33.858504089321464 14.692366856512864 6 30.6450123754026 36.7674927588211 17.915155991624264 14.672338874152038 7 28.030657662610658 33.93493857400798 20.39074257422813 17.643661189153235 8 26.442876513930557 35.39248999295082 19.55415584776856 18.61047764535006 9 26.934852951708354 16.95578755725647 20.641272483097538 35.46808700793763 10-11 24.71613903512575 27.746978349710318 26.71549920672524 20.82138340843869 12-13 25.61282187334556 25.19070550902694 27.874802132954223 21.321670484673273 14-15 22.719542382987918 25.973612448934595 26.827915703063848 24.478929465013636 16-17 21.171024943486465 29.75430557820174 27.351662884301653 21.72300659401014 18-19 21.155981921469717 27.703308745088044 30.63211824374411 20.50859108969812 20-21 22.186666429956816 26.52848729090013 30.846341279387158 20.438504999755892 22-23 21.709371870335552 26.63751440477758 30.62665035481242 21.026463370074453 24-25 21.258705878872156 26.836947838693455 30.93541229030445 20.968933992129944 26-27 21.108428002422787 27.14849985752663 30.686396145792123 21.056675994258455 28-29 20.93275558683273 27.070858386804087 31.014209083704387 20.982176942658796 30-31 21.0165545286222 27.032859006716446 30.961345147554965 20.989241317106387 32-33 20.899884584652717 27.110798733390546 30.715367997395752 21.273948684560978 34-35 21.284137182878446 27.132966378375915 30.65323644270499 20.92965999604065 36-37 21.302190772741206 26.936320718430494 30.5912281511516 21.1702603576767 38-39 21.078848217049035 27.26448294451575 30.670816105646182 20.98585273278903 40-41 21.252612481686 27.131406349484365 30.64171781174037 20.974263357089264 42-43 21.147483695068413 27.11827995806479 30.427498277111486 21.30673806975531 44-45 21.14075501766572 27.47553117738835 30.126883705076384 21.256830099869546 46-47 21.321520886986818 27.57246771322527 29.64755576806544 21.45845563172247 48-49 21.431856017811352 27.768953115595647 29.294600431198507 21.504590435394498 50-51 21.475552756695908 27.944065289714526 29.080148463195226 21.50023349039434 52-53 21.624424617832585 28.115824667083405 28.884628771949767 21.375121943134246 54-55 21.36766830725 28.07090065983348 28.985447208143007 21.57598382477351 56-57 21.40967851964793 28.391864583675385 28.83501645253083 21.363440444145855 58-59 21.487845840126404 27.979905824453162 28.97946710378983 21.552781231630604 60-61 21.543625253136376 28.056578291438555 29.155245636587136 21.244550818837933 62-63 21.479639811088408 28.013759467326594 29.276888272675578 21.229712448909428 64-65 21.33743923393338 27.83393527437625 29.265073771306582 21.563551720383792 66-67 21.263236934809175 28.22894227893176 28.894882916754316 21.612937869504748 68-69 21.28801997454955 28.13833896318265 29.024992152137003 21.5486489101308 70-71 21.35583713079245 28.19608586818599 28.90271946690508 21.545357534116484 72-73 21.395600247100006 28.202921413203757 28.73581956019039 21.665658779505847 74-75 21.294500564952695 28.371661061519337 28.70518877763409 21.62864959589388 76-77 21.422118723433844 28.274540728236282 28.75333440843193 21.550006139897945 78-79 21.410285736606475 28.29736090392658 28.597570917772643 21.6947824416943 80-81 21.249793247637992 28.62446876848381 28.477915001182076 21.647822982696123 82-83 21.437972160644986 28.504537121309852 28.400828955201625 21.656661762843537 84-85 21.391093432681792 28.34256638089783 28.344489521546272 21.9218506648741 86-87 21.424591989107284 28.27038175694767 28.524385383711166 21.78064087023388 88-89 21.538956810063045 28.200202801885283 28.480527438763687 21.78031294928799 90-91 21.327992335285117 28.330211208698667 28.600298907310624 21.74149754870559 92-93 21.43856155679463 28.205570505641663 28.546955187712754 21.80891274985095 94-95 21.280795377616187 28.14525310912594 28.57685588899462 21.997095624263256 96-97 21.440870114716734 28.109268458933208 28.447359763702934 22.002501662647123 98-99 21.31593763988879 28.044386888790463 28.34708662964209 22.292588841678658 100-101 21.502201637057084 27.67569016381221 28.862597967228094 21.959510231902616 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 2597.0 1 1511.0 2 493.5 3 585.5 4 521.0 5 481.0 6 864.5 7 1834.5 8 2970.5 9 3510.0 10 3168.5 11 2750.0 12 2830.0 13 3050.5 14 3262.0 15 3428.0 16 3546.0 17 3738.5 18 3952.5 19 4022.5 20 4405.5 21 5133.0 22 5995.0 23 7143.5 24 8454.5 25 10205.5 26 12528.0 27 15107.5 28 16970.0 29 19128.5 30 22385.0 31 25334.5 32 28891.0 33 33446.0 34 38194.5 35 42085.0 36 46812.0 37 51130.5 38 53303.5 39 54798.5 40 55483.5 41 57383.0 42 59002.5 43 60411.5 44 62702.5 45 63818.0 46 62815.0 47 61955.0 48 65000.0 49 64658.5 50 60004.0 51 56228.0 52 52333.0 53 49607.5 54 46863.0 55 42581.5 56 37806.0 57 33291.5 58 29606.0 59 26059.5 60 22214.0 61 18263.0 62 14283.5 63 10731.5 64 7827.0 65 5902.0 66 4392.5 67 3327.0 68 2483.5 69 1754.5 70 1286.5 71 952.0 72 767.5 73 625.0 74 443.0 75 338.0 76 247.5 77 152.5 78 95.0 79 63.5 80 47.0 81 31.5 82 18.5 83 15.0 84 11.5 85 10.5 86 8.5 87 6.0 88 5.0 89 4.5 90 3.5 91 3.0 92 2.0 93 2.0 94 2.0 95 1.0 96 1.0 97 0.5 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.29760182187358625 2 0.28201006141027474 3 0.26907598738957317 4 0.2570868685484662 5 0.25342516722753694 6 0.2102525274598069 7 0.20346066210647046 8 0.21515448245395413 9 0.2158631988386501 10-11 0.217723579348477 12-13 0.21382563923264913 14-15 0.2114041915849379 16-17 0.2104001767066186 18-19 0.21104983339258987 20-21 0.19900165485275825 22-23 0.19043799853768187 24-25 0.19206214025261015 26-27 0.20154122189791884 28-29 0.2155088406463021 30-31 0.20927804243084996 32-33 0.21568601974247611 34-35 0.2085102663474293 36-37 0.1946607669964954 38-39 0.19696409524675731 40-41 0.19123530447046483 42-43 0.20390360984690548 44-45 0.1977023414808156 46-47 0.19761375193272862 48-49 0.20629552764525436 50-51 0.21409140787691014 52-53 0.22974222803894634 54-55 0.20419890834052876 56-57 0.17933477517744487 58-59 0.18075220794683683 60-61 0.1863038196269553 62-63 0.19554666247736538 64-65 0.1839119018286064 66-67 0.1881937299861446 68-69 0.19203261040324782 70-71 0.185831342037158 72-73 0.18887291652147825 74-75 0.1912057746211025 76-77 0.20284053526986148 78-79 0.20975052002064729 80-81 0.19953319214128024 82-83 0.1986768265097726 84-85 0.19241649844495812 86-87 0.19285944618539314 88-89 0.19935601304510625 90-91 0.1996513115387296 92-93 0.19604866991652503 94-95 0.19714127434293133 96-97 0.18462061821330236 98-99 0.1915601328134505 100-101 0.18284882725156243 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1693202.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 55.52414118547643 #Duplication Level Percentage of deduplicated Percentage of total 1 76.80639139843535 42.64608919953687 2 13.176243880072994 14.631992509828857 3 4.326615054883399 7.206947554876611 4 1.9150958391276631 4.2533620702177135 5 0.996001838398626 2.7651073348119692 6 0.5883661677024711 1.9601115698561826 7 0.386351770866994 1.501629519101447 8 0.27503254880171235 1.221675685621417 9 0.2103480576074723 1.0511455723819219 >10 1.1709899291943513 12.124557256159488 >50 0.09952559270774897 3.845368521202188 >100 0.04679457315292967 4.474306947150064 >500 0.0016023921773838162 0.5462253855785297 >1k 3.2047843547676323E-4 0.3453871669262437 >5k 2.1365229031784216E-4 0.7983704734376164 >10k+ 1.0682614515892108E-4 0.6277232333128004 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10583 0.6250287916031283 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8055 0.47572587322717547 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5405 0.3192176716068136 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 2412 0.14245199332389166 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1943 0.11475299462202385 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 2.952984936233243E-5 0.0 0.0 0.0 0.0 14-15 1.1811939744932973E-4 0.0 0.0 0.0 0.0 16-17 1.4764924681166217E-4 0.0 0.0 5.905969872466486E-5 0.0 18-19 1.771790961739946E-4 0.0 0.0 5.905969872466486E-5 0.0 20-21 1.771790961739946E-4 0.0 0.0 1.771790961739946E-4 0.0 22-23 1.771790961739946E-4 0.0 0.0 2.3623879489865945E-4 0.0 24-25 1.771790961739946E-4 0.0 0.0 5.610671378843162E-4 0.0 26-27 1.771790961739946E-4 0.0 0.0 0.0010335447276816352 0.0 28-29 1.771790961739946E-4 0.0 0.0 0.0016536715642906162 0.0 30-31 1.771790961739946E-4 0.0 0.0 0.0035731117728422246 0.0 32-33 1.771790961739946E-4 0.0 0.0 0.006762335503974128 0.0 34-35 2.3623879489865948E-4 0.0 0.0 0.009360962247859381 0.0 36-37 2.9529849362332434E-4 0.0 0.0 0.013288432213049595 0.0 38-39 2.9529849362332434E-4 0.0 0.0 0.016536715642906163 0.0 40-41 2.9529849362332434E-4 0.0 0.0 0.021113842294067688 0.0 42-43 2.9529849362332434E-4 0.0 0.0 0.026399685329925197 0.0 44-45 2.9529849362332434E-4 0.0 0.0 0.03192176716068136 0.0 46-47 2.9529849362332434E-4 0.0 0.0 0.03909752055572814 0.0 48-49 2.9529849362332434E-4 0.0 0.0 0.045889385909064606 0.0 50-51 2.9529849362332434E-4 0.0 0.0 0.05592953469225763 0.0 52-53 2.9529849362332434E-4 0.0 0.0 0.07698431728760066 0.0 54-55 2.9529849362332434E-4 0.0 0.0 0.10477190553755547 0.0 56-57 2.9529849362332434E-4 0.0 0.0 0.12978368794745104 0.0 58-59 2.9529849362332434E-4 0.0 0.0 0.16994428308022314 0.0 60-61 2.9529849362332434E-4 0.0 0.0 0.22277318358943587 0.0 62-63 2.9529849362332434E-4 0.0 0.0 0.2616639951996277 0.0 64-65 2.9529849362332434E-4 0.0 0.0 0.2963615682003683 0.0 66-67 3.543581923479892E-4 0.0 0.0 0.3736707138309546 0.0 68-69 3.543581923479892E-4 0.0 0.0 0.4528697698207302 0.0 70-71 3.543581923479892E-4 0.0 0.0 0.4947430962165176 0.0 72-73 3.543581923479892E-4 0.0 0.0 0.5712549359143209 0.0 74-75 3.543581923479892E-4 0.0 0.0 0.6804267890068639 0.0 76-77 3.543581923479892E-4 0.0 0.0 0.7944415373948295 0.0 78-79 3.543581923479892E-4 0.0 0.0 0.9082495768372587 0.0 80-81 4.1341789107265405E-4 0.0 0.0 1.0548652789212392 0.0 82-83 4.1341789107265405E-4 0.0 0.0 1.2090110925926143 0.0 84-85 4.429477404349865E-4 0.0 0.0 1.3724292789637622 0.0 86-87 4.724775897973189E-4 0.0 0.0 1.5806147169682059 0.0 88-89 4.724775897973189E-4 0.0 0.0 1.822877601136781 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5510 0.0 45.554737 1 GTATCAA 9640 0.0 39.402245 1 ATCAACG 12155 0.0 30.886522 3 TCAACGC 12290 0.0 30.587729 4 TATCAAC 12405 0.0 30.34697 2 CAACGCA 12550 0.0 29.95315 5 AACGCAG 12905 0.0 29.228403 6 GTACATG 10520 0.0 26.11941 1 TATAACG 150 1.054379E-6 25.350447 2 ACGCAGA 14865 0.0 25.118877 7 CGCAGAG 14990 0.0 24.877724 8 TACATGG 10940 0.0 24.808798 2 ACATGGG 10940 0.0 24.369267 3 GAGTACT 8040 0.0 23.394697 12-13 GCAGAGT 15770 0.0 23.1947 9 GTACTTT 8510 0.0 21.851463 14-15 AGTACTT 8570 0.0 21.587631 12-13 CATGGGG 3550 0.0 21.553616 4 CATGGGA 7215 0.0 21.40768 4 GTACCTG 1585 0.0 21.295132 1 >>END_MODULE