FastQCFastQC Report
Thu 26 May 2016
SRR937621_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937621_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1693202
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT136880.8084091561432126No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT116080.6855649827959097No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT79170.46757563480317177No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27730.16377254456349566No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21950.1296360387006394No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA59250.053.3413051
GTATCAA108650.041.4238621
ATCAACG140000.031.998853
TCAACGC141350.031.659634
TATCAAC142850.031.526722
CAACGCA143550.031.1735025
AACGCAG147250.030.4493226
ACGCAGA169350.026.3970347
CGCAGAG170000.026.100498
TGGTATC25700.025.878892
GCAGAGT179250.024.2765549
GTACATG101700.024.253631
GTATAGG4950.024.0029811
GAGTACT107400.023.42137512-13
TACCTGG15000.023.1197072
TACATGG106300.023.1051062
GGACCGT1452.336547E-522.9293086
AGTACTT111650.022.52983312-13
ACATGGG105600.022.178583
GTGGTAT31300.022.168631
Produced by FastQC (version 0.11.5)