##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937621_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1693202 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.20479068652175 33.0 31.0 34.0 31.0 34.0 2 32.40034561735693 34.0 31.0 34.0 31.0 34.0 3 32.45932499489134 34.0 31.0 34.0 31.0 34.0 4 36.08262806209773 37.0 35.0 37.0 35.0 37.0 5 36.00806460186085 37.0 35.0 37.0 35.0 37.0 6 36.064119933711396 37.0 35.0 37.0 35.0 37.0 7 36.01883236613234 37.0 35.0 37.0 35.0 37.0 8 35.984744289222434 37.0 35.0 37.0 35.0 37.0 9 37.664433422592225 39.0 37.0 39.0 35.0 39.0 10-11 37.58818351265826 39.0 37.0 39.0 35.0 39.0 12-13 37.47786708260444 39.0 37.0 39.0 35.0 39.0 14-15 38.664141667680525 40.0 38.0 41.0 34.0 41.0 16-17 38.630930627296685 40.0 38.0 41.0 34.5 41.0 18-19 38.6519219207159 40.0 38.0 41.0 34.0 41.0 20-21 38.57422239047674 40.0 38.0 41.0 34.0 41.0 22-23 38.52475428212345 40.0 38.0 41.0 34.0 41.0 24-25 38.54140586888038 40.0 38.0 41.0 34.0 41.0 26-27 38.36387064272308 40.0 38.0 41.0 34.0 41.0 28-29 38.308728078516324 40.0 38.0 41.0 34.0 41.0 30-31 38.27145875093462 40.0 38.0 41.0 34.0 41.0 32-33 38.1012271424201 40.0 37.0 41.0 34.0 41.0 34-35 37.93317690387798 40.0 37.0 41.0 33.0 41.0 36-37 37.7530793726915 40.0 37.0 41.0 33.0 41.0 38-39 37.60869524132384 40.0 37.0 41.0 33.0 41.0 40-41 37.4099797307114 40.0 36.0 41.0 33.0 41.0 42-43 37.14068906131696 40.0 36.0 41.0 32.0 41.0 44-45 36.76896347866351 40.0 36.0 41.0 31.0 41.0 46-47 36.32784422650103 39.0 36.0 41.0 30.0 41.0 48-49 35.905484106444476 39.0 35.0 41.0 29.0 41.0 50-51 35.62508489831691 39.0 35.0 40.5 28.0 41.0 52-53 35.359114860483274 39.0 35.0 40.0 27.0 41.0 54-55 35.127035344867295 39.0 35.0 40.0 26.5 41.0 56-57 34.8576224218965 38.0 34.5 40.0 26.0 41.0 58-59 34.602250646998996 38.0 34.0 40.0 26.0 41.0 60-61 34.26938605080788 38.0 34.0 40.0 24.5 41.0 62-63 33.870668118747794 37.0 33.5 40.0 23.0 41.0 64-65 33.46516718028918 37.0 33.0 39.5 22.0 41.0 66-67 33.046904917428634 36.0 32.5 39.0 21.5 40.5 68-69 32.58353669556261 36.0 32.0 39.0 20.0 40.0 70-71 32.16671991882835 35.0 31.5 38.0 18.5 40.0 72-73 31.688338721546515 35.0 31.0 37.5 17.5 39.5 74-75 31.20993153799724 35.0 31.0 37.0 14.0 39.0 76-77 29.949257383348236 33.5 29.5 35.5 12.0 38.0 78-79 30.557442644173584 34.0 30.5 36.0 9.5 38.5 80-81 30.378549635542598 34.0 30.5 36.0 7.5 37.5 82-83 29.98133979288945 34.0 30.0 35.0 4.5 37.0 84-85 29.559882990924887 34.0 30.0 35.0 2.0 36.5 86-87 29.214328827865785 34.0 29.0 35.0 2.0 36.0 88-89 28.792326314285006 34.0 29.0 35.0 2.0 36.0 90-91 28.278387339490504 34.0 29.0 35.0 2.0 35.0 92-93 27.814238939004326 33.0 27.5 35.0 2.0 35.0 94-95 27.311298651903318 33.0 26.5 35.0 2.0 35.0 96-97 26.77265530043078 33.0 25.5 35.0 2.0 35.0 98-99 25.930029907831432 32.5 24.5 34.0 2.0 35.0 100-101 23.45159585211924 30.5 10.5 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.2005239729558781 1101 2 0.2112722576092665 1101 3 0.19370268207333652 1101 4 0.13423506089364423 1101 5 0.0755547226364186 1101 6 0.12178820509203803 1101 7 0.12725660853634224 1101 8 0.18522904376316518 1101 9 0.2839823352009887 1101 10-11 0.2408083893002697 1101 12-13 0.26785684453492564 1101 14-15 0.26848248145433473 1101 16-17 0.31658301180042514 1101 18-19 0.3757842702812013 1101 20-21 0.3627272255398637 1101 22-23 0.4127082153606523 1101 24-25 0.2888332183551512 1101 26-27 0.24543303527305227 1101 28-29 0.35980263193214057 1101 30-31 0.46007291621602064 1101 32-33 0.2795942196219485 1101 34-35 0.33693543324696407 1101 36-37 0.07879572978907134 1101 38-39 0.324601751071917 1101 40-41 0.4375690469519 1101 42-43 0.4320101313842244 1101 44-45 0.42703700904580444 1101 46-47 0.2542151517567177 1101 48-49 0.15946534202802098 1101 50-51 0.2964759644484545 1101 52-53 0.20933855567246695 1101 54-55 0.19330070987174253 1101 56-57 0.3049388995028153 1101 58-59 0.21712142257759837 1101 60-61 0.27712857569488136 1101 62-63 0.08538258944356869 1101 64-65 0.2588849874831425 1101 66-67 0.25612498167186715 1101 68-69 0.24297599831444927 1101 70-71 0.2096264182757679 1101 72-73 0.11296761026995128 1101 74-75 0.29706082156060276 1101 76-77 0.24959079263638273 1101 78-79 0.27541129535337916 1101 80-81 0.4399287472371398 1101 82-83 0.4373457073145097 1101 84-85 0.5841827939088269 1101 86-87 0.5465136997465869 1101 88-89 0.6954810743039523 1101 90-91 0.5638852657552036 1101 92-93 0.7590437501621032 1101 94-95 0.8329085541726293 1101 96-97 1.0028984013480873 1101 98-99 1.2100118390564951 1101 100-101 1.6262007678436596 1102 1 0.2574672293003317 1102 2 0.22301638839531535 1102 3 0.18297746583914432 1102 4 0.17024114396559753 1102 5 0.05873883061094176 1102 6 0.2091563259930922 1102 7 0.22555715030993895 1102 8 0.21541628832166282 1102 9 0.2635185009647003 1102 10-11 0.28062969906169855 1102 12-13 0.2759264196703697 1102 14-15 0.3978532636993677 1102 16-17 0.29232771287447434 1102 18-19 0.3198361664887841 1102 20-21 0.28493043816224173 1102 22-23 0.32561193173742 1102 24-25 0.3600165659456849 1102 26-27 0.3689878304446097 1102 28-29 0.3748107110120742 1102 30-31 0.3258518345697894 1102 32-33 0.48944594474313163 1102 34-35 0.44722532964463824 1102 36-37 0.4781047308345947 1102 38-39 0.4075910106480052 1102 40-41 0.5509451518848891 1102 42-43 0.5514702587485445 1102 44-45 0.6590852934294134 1102 46-47 0.6487422879604949 1102 48-49 0.7206372838586432 1102 50-51 0.6943155234257361 1102 52-53 0.8037649409970484 1102 54-55 0.7190520643057354 1102 56-57 0.7865319039700722 1102 58-59 0.7813196927040096 1102 60-61 0.7995741607153164 1102 62-63 0.7368159132720962 1102 64-65 0.8028351823315489 1102 66-67 0.7881732660554874 1102 68-69 0.7851478450970717 1102 70-71 0.7364936362208283 1102 72-73 1.0262771816034757 1102 74-75 0.9591062545042348 1102 76-77 0.9162070838634548 1102 78-79 0.8699735993168787 1102 80-81 0.9643532316017449 1102 82-83 1.0946292354962424 1102 84-85 1.134332969747625 1102 86-87 1.0741527074454744 1102 88-89 1.0748528070289787 1102 90-91 1.1464487102946954 1102 92-93 1.4016870350209594 1102 94-95 1.1153094666334198 1102 96-97 1.1816532725580515 1102 98-99 1.3762612450065 1102 100-101 1.992856028750417 1103 1 0.2464457079865241 1103 2 0.2680799740952011 1103 3 0.2666289747026056 1103 4 0.14660697665664912 1103 5 0.1550793179530885 1103 6 0.25433181659604287 1103 7 0.17651167690032565 1103 8 0.1529526018332561 1103 9 0.20482549094513303 1103 10-11 0.2913592300986352 1103 12-13 0.3401635394901348 1103 14-15 0.48716745709474907 1103 16-17 0.43473855949805085 1103 18-19 0.3724344352977056 1103 20-21 0.37752174070566724 1103 22-23 0.3761332900109693 1103 24-25 0.2823775370727404 1103 26-27 0.3760726706651596 1103 28-29 0.4312174519855745 1103 30-31 0.4391725940409401 1103 32-33 0.5644303818095935 1103 34-35 0.7047314807088512 1103 36-37 0.658037439674338 1103 38-39 0.6204737847038544 1103 40-41 0.6958634835384103 1103 42-43 0.6998998846452693 1103 44-45 0.8346311077413873 1103 46-47 0.8402708644708454 1103 48-49 0.835595115719677 1103 50-51 0.7551821564962253 1103 52-53 0.7707303912845944 1103 54-55 0.8590002619698112 1103 56-57 0.8886555033060546 1103 58-59 0.8942817636116942 1103 60-61 1.0362241281072642 1103 62-63 0.9153698203095075 1103 64-65 0.9399120187577026 1103 66-67 0.9981250209615169 1103 68-69 1.0687511829757668 1103 70-71 0.9397729686450944 1103 72-73 1.0731116068124606 1103 74-75 1.4235964572670312 1103 76-77 1.087186659365912 1103 78-79 1.1725659393892123 1103 80-81 1.1429664260050139 1103 82-83 1.3886986461798259 1103 84-85 1.2699161351000896 1103 86-87 1.3397845625471518 1103 88-89 1.4942960986635327 1103 90-91 1.3127330969668947 1103 92-93 1.4905235945751194 1103 94-95 1.7157804306459923 1103 96-97 1.9998489356872362 1103 98-99 2.057260385339699 1103 100-101 2.456492493444788 1104 1 0.20718881862645588 1104 2 0.23656710722534058 1104 3 0.20643265870388916 1104 4 0.14527272516266976 1104 5 0.16447153346527443 1104 6 0.15055994323279975 1104 7 0.1756550424797112 1104 8 0.18079795329000348 1104 9 0.14739548886270626 1104 10-11 0.20035010528558672 1104 12-13 0.2487272806634877 1104 14-15 0.2838741473622477 1104 16-17 0.31034731420717065 1104 18-19 0.3319856724481838 1104 20-21 0.24937325425090506 1104 22-23 0.3025422012175767 1104 24-25 0.255031889028011 1104 26-27 0.3506860019791702 1104 28-29 0.3878702515867616 1104 30-31 0.4364467060123687 1104 32-33 0.2737761371308238 1104 34-35 0.522168368996148 1104 36-37 0.47233787067346356 1104 38-39 0.4164534005908749 1104 40-41 0.5239698481901769 1104 42-43 0.5128763185219256 1104 44-45 0.5026089535661242 1104 46-47 0.46667926295888407 1104 48-49 0.3709212283978687 1104 50-51 0.3776583979062167 1104 52-53 0.3937459731965163 1104 54-55 0.5794465716581385 1104 56-57 0.4632156302078414 1104 58-59 0.5235501457558414 1104 60-61 0.5353761704625555 1104 62-63 0.4675343998171755 1104 64-65 0.7431030214143703 1104 66-67 0.7871462569761292 1104 68-69 0.7400035257052622 1104 70-71 0.7112586377664982 1104 72-73 0.702576399611047 1104 74-75 0.9490235518407317 1104 76-77 0.784702025147233 1104 78-79 0.775686265762193 1104 80-81 0.7858900814202698 1104 82-83 0.8015000063827671 1104 84-85 0.8414871127045451 1104 86-87 0.8183265013490661 1104 88-89 0.8671639781857117 1104 90-91 0.8242603909486839 1104 92-93 0.9024016669425841 1104 94-95 0.9953341209576578 1104 96-97 1.1907074395586577 1104 98-99 1.5051054686051906 1104 100-101 1.745097244216236 1105 1 0.24750768468778972 1105 2 0.1872511627968052 1105 3 0.1639199627942176 1105 4 0.18597174946486916 1105 5 0.12847404951257602 1105 6 0.1974990649755597 1105 7 0.1823153148180694 1105 8 0.2552301857067505 1105 9 0.2576013620574287 1105 10-11 0.28593197874040754 1105 12-13 0.3547701710165967 1105 14-15 0.2863458011881548 1105 16-17 0.38088917725767146 1105 18-19 0.3363118482188554 1105 20-21 0.43649989571540715 1105 22-23 0.44397899094796855 1105 24-25 0.35493555726324644 1105 26-27 0.3490440685243641 1105 28-29 0.2963354835729035 1105 30-31 0.27627107457244904 1105 32-33 0.32680216754931735 1105 34-35 0.34739999666116717 1105 36-37 0.3352280903940894 1105 38-39 0.32725064494383815 1105 40-41 0.29290929423459744 1105 42-43 0.38191597294474633 1105 44-45 0.26531142847129985 1105 46-47 0.2837970965904617 1105 48-49 0.2393233257709042 1105 50-51 0.12918372032167014 1105 52-53 0.13940406170919317 1105 54-55 0.10290300206396807 1105 56-57 0.16459278678260603 1105 58-59 0.19893815101303147 1105 60-61 0.27742963354990025 1105 62-63 0.2409771787072259 1105 64-65 0.30760445346879806 1105 66-67 0.42507304400123047 1105 68-69 0.43269310774378766 1105 70-71 0.5221186582500223 1105 72-73 0.5143655568477499 1105 74-75 0.6381070495133088 1105 76-77 0.6468131705781879 1105 78-79 0.39824165444273163 1105 80-81 0.6138347133733184 1105 82-83 0.494297133186798 1105 84-85 0.6538014885634915 1105 86-87 0.6945208005594417 1105 88-89 0.7546746545047291 1105 90-91 0.8631346973787366 1105 92-93 0.9999107967845475 1105 94-95 1.0415310587663562 1105 96-97 1.083553461715379 1105 98-99 1.3546693078866276 1105 100-101 1.7156093448781355 1106 1 -0.42425869215376366 1106 2 -0.27117832843804734 1106 3 -0.23895550745133676 1106 4 -0.20057009459270603 1106 5 -0.2200151056582058 1106 6 -0.16269343497543076 1106 7 -0.25304830907102627 1106 8 -0.2380117330198459 1106 9 -0.5766681556947333 1106 10-11 -0.6198589693779937 1106 12-13 -0.6248575709315389 1106 14-15 -0.562750596699729 1106 16-17 -0.5097530030778259 1106 18-19 -0.5148878459767445 1106 20-21 -0.5839922070106311 1106 22-23 -0.6589880780522677 1106 24-25 -0.6239356930241158 1106 26-27 -0.5897527265884577 1106 28-29 -0.5485674645169709 1106 30-31 -0.6621953680277457 1106 32-33 -0.8060389709839981 1106 34-35 -1.196731579425503 1106 36-37 -1.0049309688831372 1106 38-39 -0.8081377498147191 1106 40-41 -0.7894148637786103 1106 42-43 -1.1441072510819268 1106 44-45 -1.328328027068551 1106 46-47 -1.3225204998154467 1106 48-49 -1.5112489436862617 1106 50-51 -1.4563452803073957 1106 52-53 -1.5488505364948324 1106 54-55 -1.7946277966807358 1106 56-57 -1.906633264330928 1106 58-59 -1.856541913783289 1106 60-61 -1.8670152762146373 1106 62-63 -1.9777654145355008 1106 64-65 -2.503663128947803 1106 66-67 -2.376147411390715 1106 68-69 -2.075657927553813 1106 70-71 -2.092993710611392 1106 72-73 -2.0900532365792337 1106 74-75 -2.110471075848679 1106 76-77 -1.992282612493149 1106 78-79 -1.9708803875323113 1106 80-81 -2.1501000144946225 1106 82-83 -2.2138445526223443 1106 84-85 -2.086001021670082 1106 86-87 -2.1934103952320037 1106 88-89 -2.3364057385501624 1106 90-91 -2.409768555446302 1106 92-93 -2.386635502465378 1106 94-95 -2.3720765243274364 1106 96-97 -2.583733664739018 1106 98-99 -2.816985911905835 1106 100-101 -1.9812359779718314 1107 1 0.2181166497465128 1107 2 0.24530052794562351 1107 3 0.13937352159523186 1107 4 0.12651942127475735 1107 5 0.12478471465444585 1107 6 0.1719230903637765 1107 7 0.07545823928412432 1107 8 0.09719493345024688 1107 9 0.18518973519896775 1107 10-11 0.18374110832464652 1107 12-13 0.19051150792797245 1107 14-15 0.23236940010891516 1107 16-17 0.12932062710965653 1107 18-19 0.28551480285068465 1107 20-21 0.32183930661248183 1107 22-23 0.23814828487455486 1107 24-25 0.17063492185004492 1107 26-27 0.2877168904658731 1107 28-29 0.2689000625445317 1107 30-31 0.29008797082687465 1107 32-33 0.4270041192915457 1107 34-35 0.32967062365242583 1107 36-37 0.2518140277086971 1107 38-39 0.36072109675205155 1107 40-41 0.28058320232761247 1107 42-43 0.3373269349569199 1107 44-45 0.546524546050847 1107 46-47 0.4147736879231516 1107 48-49 0.5173156648433377 1107 50-51 0.48017010325398246 1107 52-53 0.4672783978516435 1107 54-55 0.3662745512224177 1107 56-57 0.3320283077252597 1107 58-59 0.4734493099812269 1107 60-61 0.4707167259984999 1107 62-63 0.5798006630596007 1107 64-65 0.557309810414651 1107 66-67 0.7014559305165093 1107 68-69 0.6409544368785021 1107 70-71 0.6777193325048998 1107 72-73 0.4545106573635991 1107 74-75 0.6792715254899591 1107 76-77 0.5934649805462158 1107 78-79 0.7803684048680957 1107 80-81 0.8503099623160004 1107 82-83 0.8447754103648357 1107 84-85 0.8422818714350235 1107 86-87 1.0331355056936644 1107 88-89 1.0146495343890258 1107 90-91 1.0338740505753066 1107 92-93 0.9587800418780255 1107 94-95 1.4440249192418904 1107 96-97 1.3839390892124577 1107 98-99 1.500697985783379 1107 100-101 1.6954978488657524 1108 1 0.22095497298661826 1108 2 0.21642376344508563 1108 3 0.1854507632726765 1108 4 0.12178299248289193 1108 5 0.10926167130786268 1108 6 0.1159472631632994 1108 7 0.1950816262128825 1108 8 0.16039991693274658 1108 9 0.2237366057493233 1108 10-11 0.23774671100295564 1108 12-13 0.2555509021450604 1108 14-15 0.2917111901962741 1108 16-17 0.2928710419683753 1108 18-19 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-0.379840489688533 2316 76-77 -0.2182283930088893 2316 78-79 -0.09552728297650503 2316 80-81 -0.15359782281117162 2316 82-83 -0.20760253285112285 2316 84-85 -0.1269835276922784 2316 86-87 -0.07093971640248142 2316 88-89 0.05402489763038787 2316 90-91 -0.16189113176032421 2316 92-93 0.0982431980572045 2316 94-95 -0.2524882712241947 2316 96-97 -0.047570887816849705 2316 98-99 0.0720132193187446 2316 100-101 0.6656900708112197 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4.0 3 3.0 4 17.0 5 34.0 6 82.0 7 227.0 8 480.0 9 909.0 10 1574.0 11 2665.0 12 4205.0 13 6143.0 14 8068.0 15 11634.0 16 16293.0 17 23241.0 18 21074.0 19 11917.0 20 9217.0 21 9276.0 22 9453.0 23 10211.0 24 11517.0 25 13569.0 26 16347.0 27 20152.0 28 24751.0 29 30792.0 30 39020.0 31 49065.0 32 61911.0 33 82402.0 34 113508.0 35 162398.0 36 245987.0 37 369549.0 38 283588.0 39 21917.0 40 2.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.29062901959301 20.11153973640037 15.62141246245918 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31.629569301241077 20.291908466916528 20-21 21.835649560388354 26.035037818945085 32.022934102843195 20.106378517823362 22-23 21.555873890809828 26.084325768589167 31.85638178023823 20.50341856036277 24-25 21.126924017335202 26.222742472546102 32.19701488658766 20.45331862353104 26-27 20.945115712092473 26.695387610187755 31.87519766688844 20.484299010831332 28-29 20.754136962952266 26.506831374445905 32.22704253917406 20.511989123427774 30-31 20.79412875549661 26.43651685874363 32.18305677144746 20.586297614312297 32-33 20.799887077822692 26.519200461374503 31.89744398407396 20.783468476728846 34-35 21.19859296173758 26.4278272763675 31.889668214424503 20.483911547470417 36-37 21.145417638446514 26.39421001311718 31.710389623428785 20.749982725007516 38-39 20.995575247371548 26.627596707303674 31.804651778110347 20.572176267214427 40-41 21.162342805136543 26.545283164845756 31.81825430058215 20.474119729435547 42-43 21.0800981053522 26.53951209599769 31.531023572929822 20.849366225720285 44-45 21.08829897436927 26.857516114438795 31.149295831212104 20.904889079979824 46-47 21.271147801620835 27.123432407946602 30.552408986051283 21.053010804381284 48-49 21.423876182522818 27.312600622961703 30.15425802709895 21.109265167416527 50-51 21.53836340850058 27.476875174964356 29.785371148864698 21.199390267670367 52-53 21.685274409078183 27.67977476993294 29.54904376441795 21.085907056570925 54-55 21.440560547412534 27.658601867940153 29.58654667310811 21.314290911539203 56-57 21.47747285911545 27.996511934193318 29.415805084095105 21.110210122596122 58-59 21.523362245024515 27.58743493097693 29.647673461288136 21.241529362710416 60-61 21.60852633058548 27.6566824277316 29.796326722978122 20.938464518704798 62-63 21.59582849535968 27.588025527964177 29.865633279431513 20.950512697244626 64-65 21.531748722243417 27.31629185413199 29.896403382467064 21.255556041157522 66-67 21.319399575478887 27.810975890649786 29.561889248890566 21.307735284980765 68-69 21.424525839208787 27.649713383282087 29.63036896956181 21.295391807947308 70-71 21.427921771885458 27.74099014766106 29.543787451231452 21.28730062922203 72-73 21.598870069844 27.79987266728955 29.197845265951727 21.40341199691472 74-75 21.47632119499032 28.022439141933447 29.011216617981788 21.490023045094443 76-77 21.551238422822557 27.853262634936648 29.356479616726176 21.239019325514615 78-79 21.52838231941611 27.821252278227877 29.162586625813102 21.487778776542903 80-81 21.382741102361088 28.20452019310159 29.02223125179394 21.390507452743382 82-83 21.61219679435199 28.055503222007573 28.973617395238243 21.35868258840219 84-85 21.377929771417957 27.902943510200657 28.897096886692914 21.822029831688468 86-87 21.369157371654417 27.841923172781513 29.10417658377441 21.684742871789663 88-89 21.569310690632303 27.76750795238843 29.124138761943346 21.539042595035916 90-91 21.340251124318232 27.87414369300885 29.226131199402726 21.55947398327019 92-93 21.378310378227695 27.680461385811146 29.201995086111175 21.739233149849984 94-95 21.24991583992932 27.602760922795984 29.209066608709417 21.938256628565288 96-97 21.493212268825573 27.524447762287075 29.057785190426188 21.924554778461165 98-99 21.29503060928286 27.46505055086669 28.930032001620603 22.309886838229843 100-101 21.12358090467696 27.448463655802197 29.397512079292138 22.03044336022871 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 650.0 1 487.5 2 456.5 3 640.5 4 515.0 5 408.0 6 889.5 7 2215.0 8 3759.5 9 4448.0 10 3961.5 11 3287.5 12 3242.5 13 3459.5 14 3807.0 15 4090.0 16 4222.0 17 4416.5 18 4604.0 19 4768.0 20 5096.5 21 5583.0 22 6432.0 23 7577.5 24 8798.5 25 10487.0 26 12662.5 27 15185.5 28 17185.0 29 19271.5 30 22493.0 31 25245.5 32 28227.0 33 32713.0 34 37554.5 35 41397.5 36 46540.5 37 51313.0 38 53336.0 39 54563.0 40 55631.5 41 57185.0 42 58424.5 43 60596.5 44 63230.5 45 64069.0 46 62666.5 47 61690.0 48 65089.0 49 64564.5 50 60030.0 51 56725.0 52 52328.5 53 49212.5 54 46436.0 55 42175.5 56 37479.5 57 32949.5 58 29161.0 59 25569.5 60 21621.5 61 17603.0 62 13773.0 63 10375.0 64 7592.5 65 5700.5 66 4193.5 67 3160.0 68 2223.0 69 1490.5 70 1122.5 71 837.0 72 666.5 73 580.0 74 475.5 75 378.0 76 233.0 77 111.0 78 69.0 79 41.5 80 30.0 81 21.5 82 11.0 83 7.0 84 3.5 85 0.5 86 0.0 87 1.0 88 1.5 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.041991445793236715 2 0.0 3 0.0 4 1.1811939744932974E-4 5 0.010099208481917692 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.005374432583944503 14-15 0.0 16-17 0.0 18-19 0.0 20-21 1.771790961739946E-4 22-23 0.0010926044264062999 24-25 0.0 26-27 7.382462340583108E-4 28-29 0.0012697835225802946 30-31 2.9529849362332435E-5 32-33 2.9529849362332435E-5 34-35 0.0 36-37 1.771790961739946E-4 38-39 0.0 40-41 5.905969872466487E-5 42-43 0.01937158118169008 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 1.771790961739946E-4 84-85 1.4764924681166217E-4 86-87 0.0 88-89 0.0 90-91 0.010394506975541017 92-93 0.0024805073464359245 94-95 0.0 96-97 0.0 98-99 3.8388804171032165E-4 100-101 0.0131112531168756 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1693202.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 53.27702173442452 #Duplication Level Percentage of deduplicated Percentage of total 1 75.5815332924253 40.26758991941674 2 13.632232444530047 14.52569488471909 3 4.618900018622872 7.382437100439137 4 2.0483485547545515 4.365196418853412 5 1.0783205304021464 2.872485316745567 6 0.6466698918778829 2.0671587530745557 7 0.4284644694022153 1.5979117594139347 8 0.3045131334954509 1.2978842265323884 9 0.22874016132707797 1.0967935091902656 >10 1.270973697898877 12.559475633698595 >50 0.10519922499797792 3.8799856189815936 >100 0.053210503827233066 4.980977650656538 >500 0.0022262126449267452 0.7813168897534402 >1k 3.3393189673901175E-4 0.3554621204692311 >5k 1.1131063224633726E-4 0.4695017697288793 >10k+ 2.2262126449267453E-4 1.500128428326605 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13688 0.8084091561432126 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11608 0.6855649827959097 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7917 0.46757563480317177 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2773 0.16377254456349566 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2195 0.1296360387006394 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 5.905969872466486E-5 0.0 18-19 2.952984936233243E-5 0.0 0.0 5.905969872466486E-5 0.0 20-21 5.905969872466486E-5 0.0 0.0 1.771790961739946E-4 0.0 22-23 5.905969872466486E-5 0.0 0.0 2.3623879489865945E-4 0.0 24-25 1.1811939744932973E-4 0.0 0.0 5.610671378843162E-4 5.905969872466486E-5 26-27 1.1811939744932973E-4 0.0 0.0 0.0010335447276816352 1.1811939744932973E-4 28-29 1.4764924681166217E-4 0.0 0.0 0.0016832014136529488 1.1811939744932973E-4 30-31 1.771790961739946E-4 0.0 0.0 0.0037207610196538865 1.1811939744932973E-4 32-33 1.771790961739946E-4 0.0 0.0 0.007028104148235119 1.1811939744932973E-4 34-35 2.0670894553632702E-4 0.0 0.0 0.009744850289569703 1.1811939744932973E-4 36-37 2.3623879489865945E-4 0.0 0.0 0.013731379953484581 1.1811939744932973E-4 38-39 2.3623879489865945E-4 0.0 0.0 0.01697966338334115 1.1811939744932973E-4 40-41 2.3623879489865945E-4 0.0 0.0 0.021527260185140343 1.1811939744932973E-4 42-43 2.9529849362332434E-4 0.0 0.0 0.026754043522273185 1.1811939744932973E-4 44-45 2.9529849362332434E-4 5.905969872466486E-5 0.0 0.03233518505175401 1.1811939744932973E-4 46-47 2.9529849362332434E-4 5.905969872466486E-5 0.0 0.039747177241699454 1.1811939744932973E-4 48-49 2.9529849362332434E-4 5.905969872466486E-5 0.0 0.04647998289631125 1.1811939744932973E-4 50-51 2.9529849362332434E-4 5.905969872466486E-5 0.0 0.05669731077567827 1.1811939744932973E-4 52-53 2.9529849362332434E-4 5.905969872466486E-5 0.0 0.07787021276847061 1.1811939744932973E-4 54-55 2.9529849362332434E-4 1.1811939744932973E-4 0.0 0.10574639056651244 1.1811939744932973E-4 56-57 2.9529849362332434E-4 1.1811939744932973E-4 0.0 0.13066958342832102 1.1811939744932973E-4 58-59 2.9529849362332434E-4 1.1811939744932973E-4 0.0 0.17068252931428146 1.1811939744932973E-4 60-61 2.9529849362332434E-4 1.1811939744932973E-4 0.0 0.2232456611792332 1.1811939744932973E-4 62-63 2.9529849362332434E-4 1.1811939744932973E-4 0.0 0.2623431817349613 1.1811939744932973E-4 64-65 2.9529849362332434E-4 1.1811939744932973E-4 0.0 0.2963615682003683 1.1811939744932973E-4 66-67 3.543581923479892E-4 1.1811939744932973E-4 0.0 0.3732868257892443 1.1811939744932973E-4 68-69 3.543581923479892E-4 1.1811939744932973E-4 0.0 0.4526925907245562 1.1811939744932973E-4 70-71 3.543581923479892E-4 1.1811939744932973E-4 0.0 0.49500886486077855 1.1811939744932973E-4 72-73 3.543581923479892E-4 1.1811939744932973E-4 0.0 0.5720522418471039 1.1811939744932973E-4 74-75 3.543581923479892E-4 1.1811939744932973E-4 0.0 0.6827891769558505 1.1811939744932973E-4 76-77 3.543581923479892E-4 1.1811939744932973E-4 0.0 0.797246873084251 1.1811939744932973E-4 78-79 3.543581923479892E-4 1.1811939744932973E-4 0.0 0.9117931587607385 1.1811939744932973E-4 80-81 3.543581923479892E-4 1.1811939744932973E-4 0.0 1.059737704066024 1.1811939744932973E-4 82-83 3.543581923479892E-4 1.1811939744932973E-4 0.0 1.2152418908080667 1.1811939744932973E-4 84-85 3.543581923479892E-4 1.1811939744932973E-4 0.0 1.3813472934711866 1.1811939744932973E-4 86-87 3.543581923479892E-4 1.1811939744932973E-4 0.0 1.5909796940943846 1.1811939744932973E-4 88-89 3.543581923479892E-4 1.1811939744932973E-4 0.0 1.835929794554932 1.1811939744932973E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5925 0.0 53.341305 1 GTATCAA 10865 0.0 41.423862 1 ATCAACG 14000 0.0 31.99885 3 TCAACGC 14135 0.0 31.65963 4 TATCAAC 14285 0.0 31.52672 2 CAACGCA 14355 0.0 31.173502 5 AACGCAG 14725 0.0 30.449322 6 ACGCAGA 16935 0.0 26.397034 7 CGCAGAG 17000 0.0 26.10049 8 TGGTATC 2570 0.0 25.87889 2 GCAGAGT 17925 0.0 24.276554 9 GTACATG 10170 0.0 24.25363 1 GTATAGG 495 0.0 24.002981 1 GAGTACT 10740 0.0 23.421375 12-13 TACCTGG 1500 0.0 23.119707 2 TACATGG 10630 0.0 23.105106 2 GGACCGT 145 2.336547E-5 22.929308 6 AGTACTT 11165 0.0 22.529833 12-13 ACATGGG 10560 0.0 22.17858 3 GTGGTAT 3130 0.0 22.16863 1 >>END_MODULE