FastQCFastQC Report
Thu 26 May 2016
SRR937620_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937620_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1708561
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT104580.6120940370288214No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT82620.48356482443412907No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55860.32694179487884834No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN22910.1340894472014754No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19740.11553582225042008No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA50950.050.1234741
GTATCAA92100.042.444681
ATCAACG119550.032.44693
TCAACGC120300.032.2860224
TATCAAC121600.032.0181122
CAACGCA122750.031.523585
AACGCAG126050.030.7152796
GTACATG107450.027.1752171
ACGCAGA146250.026.2780047
CGCAGAG145900.026.244138
TACATGG110500.026.1139052
GTGTACG2059.440555E-1025.5181921
TACCTGG14350.024.5146242
ACATGGG112400.024.4452323
GCAGAGT153150.024.4426869
TATAACG1000.001646192923.7692452
GTACCTG15600.023.1686131
GAGTACT84600.022.8226612-13
TGGTATC25800.022.66372
AGTACTT88350.022.36468712-13
Produced by FastQC (version 0.11.5)