##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937620_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1708561 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.623639425223917 33.0 31.0 34.0 30.0 34.0 2 31.703112151102594 33.0 31.0 34.0 30.0 34.0 3 31.74290704282727 33.0 31.0 34.0 30.0 34.0 4 35.37263580287739 37.0 35.0 37.0 33.0 37.0 5 35.389610321200124 37.0 35.0 37.0 33.0 37.0 6 35.456547937123695 37.0 35.0 37.0 33.0 37.0 7 35.3838686473588 37.0 35.0 37.0 33.0 37.0 8 35.37804854494513 37.0 35.0 37.0 33.0 37.0 9 36.91296301390469 39.0 37.0 39.0 33.0 39.0 10-11 36.833705966599965 39.0 37.0 39.0 33.0 39.0 12-13 36.71765889540964 39.0 37.0 39.0 32.5 39.0 14-15 37.7825588316718 40.0 37.5 41.0 32.5 41.0 16-17 37.68787623034823 40.0 37.0 41.0 32.0 41.0 18-19 37.63641625906245 40.0 37.0 41.0 32.0 41.0 20-21 37.66827786657895 40.0 37.5 41.0 32.0 41.0 22-23 37.6702224854717 40.0 37.0 41.0 32.0 41.0 24-25 37.59180415566081 40.0 37.0 41.0 32.0 41.0 26-27 37.48320984735108 40.0 37.0 41.0 32.0 41.0 28-29 37.338394414949185 40.0 37.0 41.0 32.0 41.0 30-31 37.073650282313594 39.0 36.5 41.0 31.0 41.0 32-33 36.89692846787443 39.0 36.0 41.0 31.0 41.0 34-35 36.83701811056204 39.0 36.0 41.0 30.5 41.0 36-37 36.650757274689056 39.0 35.5 41.0 30.0 41.0 38-39 36.4754904858533 39.0 35.0 41.0 30.0 41.0 40-41 36.207027726841474 39.0 35.0 41.0 30.0 41.0 42-43 35.91758181299936 39.0 35.0 40.0 29.0 41.0 44-45 35.60081144307988 39.0 35.0 40.0 27.5 41.0 46-47 35.15405303059124 38.5 35.0 40.0 25.5 41.0 48-49 34.72852886142198 38.0 34.0 40.0 24.0 41.0 50-51 33.84686616398244 37.5 33.5 39.5 23.0 40.5 52-53 33.961046459564514 38.0 33.5 39.5 23.0 40.5 54-55 34.21522731702292 38.0 34.0 40.0 23.0 41.0 56-57 33.9678881819262 38.0 33.5 40.0 21.5 41.0 58-59 33.66790679407993 38.0 33.0 40.0 20.0 41.0 60-61 33.30381824236887 37.5 33.0 40.0 18.5 41.0 62-63 32.9058371342902 37.0 32.0 40.0 15.5 41.0 64-65 32.46595995109335 36.0 31.5 39.0 12.0 41.0 66-67 31.954498844349136 36.0 31.0 39.0 8.0 40.0 68-69 31.449474733416015 35.0 31.0 38.5 7.0 40.0 70-71 30.967207199508827 35.0 30.0 38.0 2.0 40.0 72-73 30.451597572460102 35.0 29.5 37.5 2.0 39.5 74-75 29.873156416422944 34.0 29.0 37.0 2.0 39.0 76-77 29.29382211112158 34.0 28.5 36.0 2.0 39.0 78-79 28.73580018506802 34.0 27.5 36.0 2.0 38.0 80-81 28.1437765464622 34.0 26.5 35.0 2.0 37.0 82-83 27.55053433854571 33.0 26.0 35.0 2.0 37.0 84-85 26.954036174301063 33.0 25.5 35.0 2.0 36.0 86-87 26.318765030923682 32.0 24.5 35.0 2.0 36.0 88-89 25.715306916170974 32.0 22.0 34.5 2.0 35.0 90-91 25.077222879370417 31.5 19.0 34.0 2.0 35.0 92-93 24.341963500278887 31.0 14.5 34.0 2.0 35.0 94-95 23.51040788125212 31.0 4.5 34.0 2.0 35.0 96-97 22.54887680334504 30.0 2.0 34.0 2.0 35.0 98-99 21.293968725728845 29.5 2.0 34.0 2.0 35.0 100-101 18.137312627409848 24.0 2.0 32.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.22913921673698923 1101 2 0.1438652835972718 1101 3 0.07515636602752451 1101 4 0.13796070296938723 1101 5 0.040401966425193336 1101 6 0.14743761211087048 1101 7 0.11367793126409964 1101 8 0.1557333863753172 1101 9 0.47973196177727573 1101 10-11 0.4148625380353863 1101 12-13 0.34154076835974223 1101 14-15 0.5819161479549919 1101 16-17 0.5735696257693164 1101 18-19 0.6067351153984291 1101 20-21 0.5322277401302813 1101 22-23 0.45714797234851545 1101 24-25 0.5194181043696844 1101 26-27 0.6650299813178933 1101 28-29 0.6534638850902184 1101 30-31 0.5749971938872207 1101 32-33 0.6452367542015054 1101 34-35 0.6855123836394981 1101 36-37 0.7487375541938093 1101 38-39 0.5344779547185468 1101 40-41 0.44027705659032534 1101 42-43 0.6339378060279941 1101 44-45 0.6198040498504014 1101 46-47 0.7011504619350575 1101 48-49 0.68930200163161 1101 50-51 0.4568674640723458 1101 52-53 0.39995049429705176 1101 54-55 0.44412704739118425 1101 56-57 0.643988325340338 1101 58-59 0.6645026243072678 1101 60-61 0.6818484140876677 1101 62-63 0.6068714409961089 1101 64-65 0.8009728743349456 1101 66-67 0.7920291324657995 1101 68-69 0.9449280536062048 1101 70-71 0.993580133408706 1101 72-73 1.0342388668991909 1101 74-75 1.046983863072537 1101 76-77 0.9634502318065508 1101 78-79 1.14503231403177 1101 80-81 1.5301881251273137 1101 82-83 1.6097260948482983 1101 84-85 1.885454939502548 1101 86-87 2.1688720386697007 1101 88-89 2.5232956472995376 1101 90-91 2.5316089891985207 1101 92-93 2.3216074891771115 1101 94-95 2.7947794139828304 1101 96-97 2.6012952421473194 1101 98-99 3.074235421467801 1101 100-101 3.9410102670604203 1102 1 0.3706581742489803 1102 2 0.2734645422384965 1102 3 0.2432964089550005 1102 4 0.31270546282392075 1102 5 0.3340204851088373 1102 6 0.3274312748945363 1102 7 0.30950508130008814 1102 8 0.17666928813891047 1102 9 0.49651205081676153 1102 10-11 0.4956978723155814 1102 12-13 0.48367234998882225 1102 14-15 0.49275764430233693 1102 16-17 0.5692840434141715 1102 18-19 0.6004436766910928 1102 20-21 0.7146149758938378 1102 22-23 0.5759910800684551 1102 24-25 0.639809690202334 1102 26-27 0.6162123179992278 1102 28-29 0.7535481159304851 1102 30-31 0.7581116968774992 1102 32-33 0.7184383700324446 1102 34-35 0.7222431602191861 1102 36-37 0.7779930427326818 1102 38-39 1.1142934430026585 1102 40-41 1.1921782214917016 1102 42-43 1.3078458304087661 1102 44-45 1.3420803206374288 1102 46-47 1.1167544241283096 1102 48-49 1.2028935791844901 1102 50-51 1.0787159727565552 1102 52-53 1.076094059315622 1102 54-55 0.9706904131855012 1102 56-57 0.9969593333717199 1102 58-59 1.0124169954579614 1102 60-61 1.1784087227323852 1102 62-63 1.3829786227500236 1102 64-65 1.5925755850075873 1102 66-67 1.525447280470047 1102 68-69 1.4369026378656997 1102 70-71 1.4842108456608685 1102 72-73 1.462702760688721 1102 74-75 1.550265124019358 1102 76-77 1.5587254230376324 1102 78-79 1.7197975344968377 1102 80-81 1.8806517132018854 1102 82-83 1.9664632086443241 1102 84-85 2.270698380961317 1102 86-87 2.2583716851867806 1102 88-89 2.370954459851685 1102 90-91 2.365178243190332 1102 92-93 2.6456199661688764 1102 94-95 2.7439499233166877 1102 96-97 2.923775163680478 1102 98-99 3.3851805742934324 1102 100-101 3.709144577832415 1103 1 0.17887170310135758 1103 2 0.01724767685177042 1103 3 0.30908743006380135 1103 4 0.23579538647460652 1103 5 0.2834179384701798 1103 6 0.1396386758635657 1103 7 0.12968581803241364 1103 8 0.13914651691503366 1103 9 0.09171075919477545 1103 10-11 0.186719331589849 1103 12-13 0.25288020346168594 1103 14-15 0.38119013516853784 1103 16-17 0.5190109444949584 1103 18-19 0.49108923066561516 1103 20-21 0.4668399162544503 1103 22-23 0.38080674121862756 1103 24-25 0.3561187931621461 1103 26-27 0.3416443189774441 1103 28-29 0.21216445887387891 1103 30-31 0.27419914305017556 1103 32-33 0.36740442601363554 1103 34-35 0.39446507090692506 1103 36-37 0.5294177288811639 1103 38-39 0.6933649845609722 1103 40-41 0.6406034925461768 1103 42-43 0.5485811841990085 1103 44-45 0.6659552722522335 1103 46-47 0.530640256085043 1103 48-49 0.7633338007789732 1103 50-51 0.5423881309973879 1103 52-53 0.6292611189051556 1103 54-55 0.9123824504419105 1103 56-57 1.0523700253910704 1103 58-59 1.0037378393737342 1103 60-61 1.0406524564109105 1103 62-63 1.1986025834904481 1103 64-65 1.159705036153916 1103 66-67 1.4663824836333745 1103 68-69 1.3619486086733659 1103 70-71 1.4508112333655347 1103 72-73 1.4767866506952387 1103 74-75 1.552796731140102 1103 76-77 1.4727578709120657 1103 78-79 1.7243188734409074 1103 80-81 1.7803041185985968 1103 82-83 2.018439232950243 1103 84-85 1.9468981976232733 1103 86-87 2.197914913322709 1103 88-89 2.171681230866831 1103 90-91 2.3838687547065938 1103 92-93 2.3061462881070547 1103 94-95 2.760181259480312 1103 96-97 2.720291546110868 1103 98-99 2.892744631625124 1103 100-101 3.5222301166352032 1104 1 0.230131198088376 1104 2 0.17810695127237253 1104 3 0.19185349803906604 1104 4 0.3823415983320757 1104 5 0.2618749393121007 1104 6 0.3023371720087127 1104 7 0.3289530110365959 1104 8 0.20803691674618818 1104 9 0.42122974215929077 1104 10-11 0.34231016842600326 1104 12-13 0.34722316554716315 1104 14-15 0.4764464774265562 1104 16-17 0.4805136544555708 1104 18-19 0.5116732877324992 1104 20-21 0.37239414778844093 1104 22-23 0.25290450541350395 1104 24-25 0.18905686711575953 1104 26-27 0.2749325187520455 1104 28-29 0.2099471121663754 1104 30-31 0.471411571406982 1104 32-33 0.2620393737965685 1104 34-35 0.48635859309246854 1104 36-37 0.4320080991944124 1104 38-39 0.5484214229273832 1104 40-41 0.5102459755694966 1104 42-43 0.5057755669206827 1104 44-45 0.7150414247779509 1104 46-47 0.5819363563742357 1104 48-49 0.5498195515809741 1104 50-51 0.5078251321041165 1104 52-53 0.5710752387384588 1104 54-55 0.6375661973762163 1104 56-57 0.7664072631082348 1104 58-59 0.9217645487829387 1104 60-61 0.8769658118164614 1104 62-63 0.8252621861126315 1104 64-65 0.8039934018206267 1104 66-67 0.8626492879982806 1104 68-69 0.8691485600739881 1104 70-71 0.9550389510561033 1104 72-73 0.9112598497727866 1104 74-75 0.9890982482351696 1104 76-77 0.9363916714692486 1104 78-79 1.061077333744013 1104 80-81 1.0785814497138055 1104 82-83 1.1684707368751894 1104 84-85 1.4723922329061097 1104 86-87 1.8283214969810082 1104 88-89 1.784693481181673 1104 90-91 1.8761569132028804 1104 92-93 2.0835120615264664 1104 94-95 2.5105747664785483 1104 96-97 2.4316170708322993 1104 98-99 2.969873171533081 1104 100-101 3.762155870178713 1105 1 0.26257238630625324 1105 2 0.2448225689221637 1105 3 0.20858545509668147 1105 4 0.3283356193191409 1105 5 0.18721917634244534 1105 6 0.22263918881611744 1105 7 0.24572087444721546 1105 8 0.22147799603467178 1105 9 0.3764916366430313 1105 10-11 0.43185496462718476 1105 12-13 0.4396753677362426 1105 14-15 0.48398802188386725 1105 16-17 0.4707634658463675 1105 18-19 0.6252452840046985 1105 20-21 0.6305582035118746 1105 22-23 0.4638948752489327 1105 24-25 0.4563603000051799 1105 26-27 0.5362740849952345 1105 28-29 0.6029558628270095 1105 30-31 0.6576669018866355 1105 32-33 0.6693221101027262 1105 34-35 0.8019280315413084 1105 36-37 0.747190636603591 1105 38-39 0.826966570123254 1105 40-41 0.8264278392302487 1105 42-43 0.8931054656163582 1105 44-45 0.9920807240795995 1105 46-47 0.9658547480923758 1105 48-49 0.9414826208883014 1105 50-51 0.9210061929149518 1105 52-53 0.9078915555535261 1105 54-55 1.0397439782022246 1105 56-57 1.1541222473538184 1105 58-59 1.297296991584922 1105 60-61 1.323314660190789 1105 62-63 1.4149310407806652 1105 64-65 1.3903937697598892 1105 66-67 1.5474439561065836 1105 68-69 1.4677627362249055 1105 70-71 1.4626093416284647 1105 72-73 1.6043567525472575 1105 74-75 1.6080650731856458 1105 76-77 1.8039024736333111 1105 78-79 2.0729819035334565 1105 80-81 2.0496980162595726 1105 82-83 2.1501363075239652 1105 84-85 2.3940780563517485 1105 86-87 2.533648345653887 1105 88-89 2.5297075411412493 1105 90-91 2.5123200571470115 1105 92-93 2.723179705867988 1105 94-95 2.8638743182542967 1105 96-97 3.0787088579600734 1105 98-99 3.2992058378420275 1105 100-101 3.5996552145851908 1106 1 0.09372503557133882 1106 2 0.10825792793065858 1106 3 0.05078238542342106 1106 4 0.04529087447304647 1106 5 0.05597604770230191 1106 6 0.12629832381672657 1106 7 0.15766394490725588 1106 8 0.2518716123731366 1106 9 0.159878215466712 1106 10-11 0.04650434152036809 1106 12-13 -0.08170198923077265 1106 14-15 -0.04496268652616209 1106 16-17 0.04406107673535331 1106 18-19 0.21380947777549153 1106 20-21 0.2014635976975896 1106 22-23 0.2055551407241225 1106 24-25 -0.06932756064416168 1106 26-27 0.1139014549026669 1106 28-29 0.3643178569344627 1106 30-31 0.39942862020797776 1106 32-33 0.1578210841867076 1106 34-35 0.25317269232647277 1106 36-37 0.09124436106180411 1106 38-39 -0.058986144568564214 1106 40-41 0.15038292667639297 1106 42-43 0.26635835443439504 1106 44-45 0.17927344100752407 1106 46-47 0.4879118391286994 1106 48-49 0.34771244678280055 1106 50-51 0.32542974405443204 1106 52-53 0.5178486347882014 1106 54-55 0.483428538528365 1106 56-57 0.36808422945256325 1106 58-59 0.1813790400214117 1106 60-61 0.4348929144978726 1106 62-63 0.37482445317819924 1106 64-65 0.5009893807855335 1106 66-67 0.7538207851047076 1106 68-69 0.5925894899615827 1106 70-71 0.5336816153937889 1106 72-73 0.46214387963040693 1106 74-75 0.6762749273627087 1106 76-77 1.106990366996019 1106 78-79 1.314845488794223 1106 80-81 0.9691165051090742 1106 82-83 1.2624342558989596 1106 84-85 1.4556847795697472 1106 86-87 1.512177610659073 1106 88-89 1.5702346404387413 1106 90-91 1.8485111860273236 1106 92-93 2.13662697005741 1106 94-95 1.9474234286784728 1106 96-97 2.15709194804813 1106 98-99 2.259347706189363 1106 100-101 2.7547635724362927 1107 1 0.16271880339398948 1107 2 0.26366936005018715 1107 3 0.2343782400563228 1107 4 0.20951876781969503 1107 5 0.26906468785894333 1107 6 0.21244330982143111 1107 7 0.3024113561061199 1107 8 0.3548910316669307 1107 9 0.3638029759697545 1107 10-11 0.3275321744178612 1107 12-13 0.38134866486859664 1107 14-15 0.46580749002482236 1107 16-17 0.48597218196946557 1107 18-19 0.5515773924107563 1107 20-21 0.49758339381307337 1107 22-23 0.616994652137187 1107 24-25 0.6749866452378015 1107 26-27 0.6479708416721834 1107 28-29 0.6241174383897103 1107 30-31 0.4944247682164402 1107 32-33 0.5450217951214285 1107 34-35 0.6821326053322352 1107 36-37 0.613708288680499 1107 38-39 0.8948872834246515 1107 40-41 0.7858253964846611 1107 42-43 0.8833469922913295 1107 44-45 0.8725865397499177 1107 46-47 0.9408832522593116 1107 48-49 1.0068927117735385 1107 50-51 0.8768712841282849 1107 52-53 0.9803011624831086 1107 54-55 1.0684277829138225 1107 56-57 1.0901303925733217 1107 58-59 1.1009537275912251 1107 60-61 1.1764226397170034 1107 62-63 1.4802090691946006 1107 64-65 1.402889053243058 1107 66-67 1.529254606411726 1107 68-69 1.5668930515343078 1107 70-71 1.6483943750952221 1107 72-73 1.8413260418707047 1107 74-75 1.9128837128601646 1107 76-77 1.9606175632061174 1107 78-79 1.8848492750801462 1107 80-81 2.0651189756401074 1107 82-83 2.241595720871299 1107 84-85 2.2819039061450432 1107 86-87 2.423148558922474 1107 88-89 2.2874557529661743 1107 90-91 2.7586967468904326 1107 92-93 3.1447081444138227 1107 94-95 3.5807834412282453 1107 96-97 3.7132827719308032 1107 98-99 3.8938154743011175 1107 100-101 4.251127588100513 1108 1 0.11767519743936461 1108 2 0.15669504961038072 1108 3 0.22823088940231528 1108 4 0.11101062633334635 1108 5 0.14615891067047926 1108 6 0.15530077295734657 1108 7 0.2214244651162005 1108 8 0.1299372318796017 1108 9 0.4729211524305441 1108 10-11 0.38448574680726466 1108 12-13 0.3205803721466154 1108 14-15 0.35200343497530895 1108 16-17 0.29669403426971286 1108 18-19 0.3460464983205398 1108 20-21 0.37681968107751374 1108 22-23 0.3435750677309173 1108 24-25 0.43029106220232904 1108 26-27 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2109.0 8 2729.0 9 3388.0 10 4433.0 11 5556.0 12 7595.0 13 9619.0 14 11171.0 15 13973.0 16 17896.0 17 23202.0 18 20428.0 19 14448.0 20 14039.0 21 15205.0 22 16521.0 23 18147.0 24 20674.0 25 23597.0 26 26930.0 27 31348.0 28 36841.0 29 43094.0 30 52077.0 31 62799.0 32 78113.0 33 99836.0 34 129588.0 35 173817.0 36 235205.0 37 289459.0 38 177837.0 39 13747.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.86208321908377 19.569114278718818 14.892349027650646 21.676453474546765 2 14.846202060655287 20.50830905603771 41.46076937703794 23.184719506269058 3 19.133225890889968 25.854325888659734 29.92080325942655 25.091644961023746 4 12.152142400215972 16.86947233747865 37.76962668654229 33.208758575763085 5 12.995326743550725 38.44793658972588 33.870105419698106 14.686631247025295 6 30.647550616832163 36.825020936402595 17.892120640595273 14.635307806169964 7 28.015382576499466 33.95285069072647 20.33957541930891 17.69219131346515 8 26.44804615591226 35.37820413951162 19.561253758407485 18.61249594616864 9 27.003231349366924 16.9369036518353 20.588211146102733 35.47165385269504 10-11 24.739161275320363 27.715336915280965 26.75772355625645 20.78777825314222 12-13 25.632754655322422 25.191129133238064 27.91873855798417 21.25737765345534 14-15 22.725834577841297 26.043180150115074 26.775516762811197 24.455468509232425 16-17 21.203274401956033 29.78534130735063 27.31159566255211 21.69978862814122 18-19 21.154499061504442 27.641875255440258 30.754185701913173 20.44943998114213 20-21 22.135726266491265 26.532702830198364 30.941875870941338 20.38969503236904 22-23 21.72386312257992 26.653308023454446 30.680575371658215 20.94225348230742 24-25 21.266475323774397 26.833802894537694 30.995144300277573 20.904577481410332 26-27 21.061361715129003 27.186156426910358 30.751991893149555 21.000489964811084 28-29 20.905377548536926 27.109366065642508 31.050145295561375 20.935111090259188 30-31 21.057358916372646 26.95655590796487 31.076401921777645 20.909683253884843 32-33 20.82038824844828 27.11431662121042 30.83877698894418 21.226518141397126 34-35 21.3030865415715 27.09476147985328 30.725924761682183 20.876227216893035 36-37 21.307512371672665 26.936117660925568 30.576811096183853 21.17955887121791 38-39 21.07146762720277 27.253521312578922 30.69003155497141 20.984979505246898 40-41 21.27473444986737 27.113084837667895 30.703611106550742 20.90856960591399 42-43 21.110929006128014 27.111226303100423 30.47868624057423 21.299158450197332 44-45 21.128249663778597 27.444960695740082 30.1738441298983 21.25294551058302 46-47 21.277713075328897 27.609570958256953 29.660687456677703 21.452028509736447 48-49 21.412548361386897 27.77112139771079 29.35510526757182 21.461224973330495 50-51 21.421171787331506 27.97196338138932 29.121624256648126 21.48524057463105 52-53 21.58445387478655 28.05013910422568 29.004470044718044 21.36093697626973 54-55 21.409374050707886 28.06641145003161 29.034850675983954 21.489363823276555 56-57 21.371101314663928 28.42817835527176 28.866086970796907 21.334633359267404 58-59 21.45879674314437 27.989947701433604 29.08240534129482 21.468850214127208 60-61 21.57092555877603 28.031658429490726 29.21410041918279 21.183315592550453 62-63 21.45404509513198 28.011506241194876 29.34446201316423 21.189986650508917 64-65 21.367630611918838 27.88451418947541 29.259503012909114 21.488352185696634 66-67 21.279822934912456 28.279522451941048 28.906579111303287 21.534075501843205 68-69 21.2717213406703 28.143575654333542 29.039091304310865 21.545611700685296 70-71 21.319353058041088 28.213815308278324 28.964023373681947 21.502808259998645 72-73 21.464521567046578 28.202997764503245 28.69949792941694 21.63298273903324 74-75 21.243988023544784 28.384063996397213 28.73328931489563 21.638658665162378 76-77 21.40621344261968 28.30909710343873 28.72290832413199 21.561781129809596 78-79 21.385942208381245 28.28759536602199 28.67313473706801 21.653327688528755 80-81 21.25843593963152 28.557783053095516 28.572706006386085 21.611075000886874 82-83 21.408743612497005 28.520327291094826 28.46961130151594 21.601317794892225 84-85 21.327110913077636 28.35192828582484 28.439020672349706 21.881940128747814 86-87 21.357756112461733 28.249169570592294 28.607080174730964 21.785994142215014 88-89 21.545890179028206 28.169450132882233 28.482093514496842 21.802566173592716 90-91 21.33380474809653 28.27409719528738 28.69244848078677 21.699649575829323 92-93 21.46351545071046 28.16983615592304 28.590249093419562 21.776399299946938 94-95 21.29564048833285 28.05278389829018 28.582493236817513 22.06908237655946 96-97 21.491969246718156 27.9550955645568 28.540984048728657 22.011951139996388 98-99 21.26474456436654 28.007973220112593 28.361485089937666 22.3657971255832 100-101 21.650857394108165 27.342840392789096 29.090019053202404 21.91628315990034 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 2415.0 1 1431.0 2 489.5 3 555.0 4 504.5 5 516.0 6 890.0 7 1827.5 8 3012.5 9 3589.0 10 3231.5 11 2795.5 12 2878.5 13 3124.5 14 3359.5 15 3493.5 16 3640.5 17 3831.0 18 3988.0 19 4195.0 20 4591.0 21 5254.5 22 6117.0 23 7241.5 24 8637.0 25 10290.0 26 12558.5 27 15400.5 28 17397.0 29 19491.0 30 22787.5 31 25714.5 32 29217.5 33 33774.5 34 38544.0 35 42431.5 36 47176.5 37 51916.5 38 54159.0 39 55470.0 40 56025.0 41 57700.5 42 59564.5 43 61251.5 44 63284.5 45 64245.5 46 63397.0 47 62412.5 48 64988.5 49 64510.0 50 60209.5 51 56524.0 52 52635.5 53 50202.5 54 47264.0 55 42828.0 56 38238.0 57 33678.0 58 29746.0 59 26104.0 60 22166.5 61 18290.0 62 14527.5 63 10923.5 64 7920.0 65 5960.5 66 4537.0 67 3373.5 68 2483.5 69 1754.0 70 1281.0 71 952.0 72 753.0 73 617.5 74 455.5 75 353.0 76 248.5 77 142.0 78 91.0 79 61.0 80 37.5 81 24.5 82 12.5 83 7.0 84 8.0 85 5.0 86 3.5 87 6.0 88 6.0 89 3.5 90 5.0 91 3.5 92 0.5 93 2.0 94 3.0 95 1.5 96 0.5 97 1.0 98 1.0 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.31710895894264235 2 0.2713979775963516 3 0.27526087742843247 4 0.2710468048843442 5 0.26981770039231845 6 0.19882228378149802 7 0.19051119626399057 8 0.1980614095721487 9 0.19847111106949064 10-11 0.20113417080221307 12-13 0.20464589792228666 14-15 0.19914419210083806 16-17 0.19153545000734534 18-19 0.18565330708122216 20-21 0.18439493819652913 22-23 0.19302793403337662 24-25 0.18995517280331226 26-27 0.19528129226875715 28-29 0.1999343307028546 30-31 0.2032704714669245 32-33 0.20142681472888588 34-35 0.20090005566087485 36-37 0.19045266747865602 38-39 0.18287318977783057 40-41 0.17822015134373312 42-43 0.1870287335365843 44-45 0.18796519410193724 46-47 0.19425703852540238 48-49 0.20075373369753843 50-51 0.1968030406874557 52-53 0.2074552796183455 54-55 0.1956617293734318 56-57 0.17289403187828822 58-59 0.15700346665995538 60-61 0.17444504468965405 62-63 0.1684458441928617 64-65 0.17462063104565773 66-67 0.17403534319231212 68-69 0.17274770991495184 70-71 0.17368417048030477 72-73 0.18281466099249602 74-75 0.1881700448506082 76-77 0.18694094035858244 78-79 0.18483390408653833 80-81 0.18345847763117618 82-83 0.17467915983099228 84-85 0.16979200625555657 86-87 0.17368417048030477 88-89 0.18164408528580483 90-91 0.17889323237508054 92-93 0.1741816651556485 94-95 0.17576194235968162 96-97 0.1824342238878214 98-99 0.181556292107803 100-101 0.16452441557544625 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1708561.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 55.47285783538061 #Duplication Level Percentage of deduplicated Percentage of total 1 76.8874961962563 42.65169145813292 2 13.089529383343246 14.522272052284741 3 4.287024823458408 7.134405557053677 4 1.9237767340919858 4.268695731091901 5 1.0030103580186163 2.7819925498890457 6 0.6075759266593567 2.022238380226448 7 0.4117166088932661 1.5987367839520805 8 0.2631310066915815 1.1677303141026147 9 0.20828681390637804 1.0398838335131597 >10 1.1691967707377862 12.114375790315874 >50 0.09815583588201626 3.770599127610895 >100 0.04876745945748189 4.5875358280341505 >500 0.0016960600753702687 0.5706273411113622 >1k 3.1801126413192536E-4 0.3399419475437742 >5k 2.120075094212836E-4 0.814308266664277 >10k+ 1.060037547106418E-4 0.6149650384730653 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10458 0.6120940370288214 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8262 0.48356482443412907 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5586 0.32694179487884834 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 2291 0.1340894472014754 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1974 0.11553582225042008 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 5.852878533455932E-5 0.0 0.0 0.0 0.0 12-13 5.852878533455932E-5 0.0 0.0 5.852878533455932E-5 0.0 14-15 5.852878533455932E-5 0.0 0.0 5.852878533455932E-5 0.0 16-17 5.852878533455932E-5 0.0 0.0 5.852878533455932E-5 0.0 18-19 5.852878533455932E-5 0.0 0.0 1.1705757066911864E-4 0.0 20-21 5.852878533455932E-5 0.0 0.0 2.3411514133823727E-4 0.0 22-23 5.852878533455932E-5 0.0 0.0 4.097014973419152E-4 0.0 24-25 5.852878533455932E-5 0.0 0.0 5.852878533455932E-4 0.0 26-27 5.852878533455932E-5 0.0 0.0 7.901386020165508E-4 0.0 28-29 5.852878533455932E-5 0.0 0.0 0.0016388059893676608 0.0 30-31 5.852878533455932E-5 0.0 0.0 0.0031312900153989236 0.0 32-33 5.852878533455932E-5 0.0 0.0 0.00596993610412505 0.0 34-35 5.852878533455932E-5 0.0 0.0 0.008281823124840142 0.0 36-37 5.852878533455932E-5 0.0 0.0 0.010476652574886117 0.0 38-39 1.1705757066911864E-4 0.0 0.0 0.013022654736939449 0.0 40-41 1.463219633363983E-4 0.0 0.0 0.016885554569020363 0.0 42-43 1.7558635600367796E-4 0.0 0.0 0.021919030107792465 0.0 44-45 1.7558635600367796E-4 0.0 0.0 0.02645501097122081 0.0 46-47 1.7558635600367796E-4 0.0 0.0 0.031956716792669385 0.0 48-49 1.7558635600367796E-4 0.0 0.0 0.03760474457745436 0.0 50-51 1.7558635600367796E-4 0.0 0.0 0.04620847602163458 0.0 52-53 1.7558635600367796E-4 0.0 0.0 0.06523033125536637 0.0 54-55 1.7558635600367796E-4 0.0 0.0 0.0870323037924897 0.0 56-57 1.7558635600367796E-4 0.0 0.0 0.10830751726160201 0.0 58-59 1.7558635600367796E-4 0.0 0.0 0.14474168613236518 0.0 60-61 1.7558635600367796E-4 0.0 0.0 0.19127207047333986 0.0 62-63 1.7558635600367796E-4 0.0 0.0 0.22363848876335113 0.0 64-65 1.7558635600367796E-4 0.0 0.0 0.2514689261899341 0.0 66-67 1.7558635600367796E-4 0.0 0.0 0.3227277223347601 0.0 68-69 1.7558635600367796E-4 0.0 0.0 0.39708854410231764 0.0 70-71 1.7558635600367796E-4 0.0 0.0 0.4333471266170772 0.0 72-73 1.7558635600367796E-4 0.0 0.0 0.5008893448931586 0.0 74-75 1.7558635600367796E-4 0.0 0.0 0.6062411584953653 0.0 76-77 1.7558635600367796E-4 0.0 0.0 0.7131732493016052 0.0 78-79 1.7558635600367796E-4 0.0 0.0 0.8196371098251687 0.0 80-81 2.926439266727966E-4 0.0 0.0 0.954136258523986 0.0 82-83 2.926439266727966E-4 0.0 0.0 1.101979970279083 0.0 84-85 3.511727120073559E-4 2.926439266727966E-5 0.0 1.2532476159762513 0.0 86-87 4.097014973419152E-4 5.852878533455932E-5 0.0 1.4544110511711317 0.0 88-89 4.097014973419152E-4 5.852878533455932E-5 0.0 1.6856290176353084 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5095 0.0 50.123474 1 GTATCAA 9210 0.0 42.44468 1 ATCAACG 11955 0.0 32.4469 3 TCAACGC 12030 0.0 32.286022 4 TATCAAC 12160 0.0 32.018112 2 CAACGCA 12275 0.0 31.52358 5 AACGCAG 12605 0.0 30.715279 6 GTACATG 10745 0.0 27.175217 1 ACGCAGA 14625 0.0 26.278004 7 CGCAGAG 14590 0.0 26.24413 8 TACATGG 11050 0.0 26.113905 2 GTGTACG 205 9.440555E-10 25.518192 1 TACCTGG 1435 0.0 24.514624 2 ACATGGG 11240 0.0 24.445232 3 GCAGAGT 15315 0.0 24.442686 9 TATAACG 100 0.0016461929 23.769245 2 GTACCTG 1560 0.0 23.168613 1 GAGTACT 8460 0.0 22.82266 12-13 TGGTATC 2580 0.0 22.6637 2 AGTACTT 8835 0.0 22.364687 12-13 >>END_MODULE