FastQCFastQC Report
Thu 26 May 2016
SRR937620_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937620_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1708561
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT137960.8074631224755803No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT116960.6845526732730057No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT79450.4650111994830738No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27910.16335383986875507No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22800.13344563056279524No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA63900.047.447781
GTATCAA110100.039.1485821
CCGTCCG756.92297E-638.001039
ATCAACG141600.030.1941493
TATCAAC143350.029.924962
TCAACGC143550.029.7508954
CAACGCA146550.029.1418675
AACGCAG150850.028.3078616
GTACATG101500.025.70411
GTATAGG5100.025.1587851
ACCGTCC1701.1232078E-725.1477418
TACCTGG12500.025.0828822
TACATGG103800.024.6680562
CGCAGAG172400.024.5221258
ACGCAGA173300.024.5044147
ACATGGG105100.023.730133
GCAGAGT180350.023.0460919
GAGTACT108800.022.7243212-13
TACACCG1954.219255E-721.9255965
AGTACTT111850.021.89231712-13
Produced by FastQC (version 0.11.5)