##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937620_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1708561 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.15621040161867 33.0 31.0 34.0 31.0 34.0 2 32.35217355423658 34.0 31.0 34.0 31.0 34.0 3 32.46439255022209 34.0 31.0 34.0 31.0 34.0 4 36.11579100775448 37.0 35.0 37.0 35.0 37.0 5 35.95989256456164 37.0 35.0 37.0 35.0 37.0 6 36.06431611162844 37.0 35.0 37.0 35.0 37.0 7 36.025001741231364 37.0 35.0 37.0 35.0 37.0 8 36.02373342245316 37.0 35.0 37.0 35.0 37.0 9 37.66462830416941 39.0 37.0 39.0 35.0 39.0 10-11 37.62551059049106 39.0 37.0 39.0 35.0 39.0 12-13 37.542241102307734 39.0 37.0 39.0 35.0 39.0 14-15 38.73188695048055 40.0 38.0 41.0 34.5 41.0 16-17 38.707813183140665 40.0 38.0 41.0 34.5 41.0 18-19 38.740575548663465 40.0 38.0 41.0 35.0 41.0 20-21 38.684547990970174 40.0 38.0 41.0 34.0 41.0 22-23 38.53406082662545 40.0 38.0 41.0 34.0 41.0 24-25 38.480637799879545 40.0 38.0 41.0 34.0 41.0 26-27 38.40248431282231 40.0 38.0 41.0 34.0 41.0 28-29 38.28958111533624 40.0 38.0 41.0 34.0 41.0 30-31 38.295863009866196 40.0 38.0 41.0 34.0 41.0 32-33 38.17609028884541 40.0 37.0 41.0 34.0 41.0 34-35 38.02712399498759 40.0 37.0 41.0 33.5 41.0 36-37 37.852455370337964 40.0 37.0 41.0 33.0 41.0 38-39 37.71358148757932 40.0 37.0 41.0 33.0 41.0 40-41 37.50614815625547 40.0 37.0 41.0 33.0 41.0 42-43 37.268159579903795 40.0 36.5 41.0 32.5 41.0 44-45 36.906783837392986 40.0 36.0 41.0 31.5 41.0 46-47 36.45687394245801 39.5 36.0 41.0 30.5 41.0 48-49 36.04401657301086 39.0 35.5 41.0 29.5 41.0 50-51 35.76862546903506 39.0 35.0 41.0 28.5 41.0 52-53 35.53964915504919 39.0 35.0 40.0 28.0 41.0 54-55 35.333795515641526 39.0 35.0 40.0 27.0 41.0 56-57 35.065346218250326 38.0 35.0 40.0 27.0 41.0 58-59 34.813308099623015 38.0 34.0 40.0 26.0 41.0 60-61 34.477663952296695 38.0 34.0 40.0 26.0 41.0 62-63 34.11971770396258 37.5 34.0 40.0 25.0 41.0 64-65 33.7131194028191 37.0 33.0 39.5 23.5 41.0 66-67 33.2931826841418 36.0 33.0 39.0 22.5 40.5 68-69 32.8525621853712 36.0 32.0 39.0 22.0 40.0 70-71 32.44359463899738 35.0 32.0 38.5 20.5 40.0 72-73 32.00217112529199 35.0 31.5 38.0 19.5 39.5 74-75 31.503957423820395 35.0 31.0 37.0 18.0 39.0 76-77 30.26853796850098 33.5 29.5 36.0 17.5 38.5 78-79 30.83126999855434 34.0 31.0 36.0 17.5 38.5 80-81 30.6206275339306 34.0 31.0 36.0 13.0 37.5 82-83 30.258284603242146 34.0 30.5 35.0 8.5 37.0 84-85 29.840764830755237 34.0 30.0 35.0 7.0 36.5 86-87 29.410328633276777 34.0 29.5 35.0 2.0 36.0 88-89 28.963494133367202 34.0 29.0 35.0 2.0 36.0 90-91 28.438756356957697 34.0 29.0 35.0 2.0 35.0 92-93 27.99569462255079 33.0 28.0 35.0 2.0 35.0 94-95 27.51443232053172 33.0 27.0 35.0 2.0 35.0 96-97 26.93832704831727 33.0 26.0 35.0 2.0 35.0 98-99 26.08204711450162 32.5 24.5 34.0 2.0 35.0 100-101 23.61985524660811 30.5 11.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.2897272903992487 1101 2 0.26126949720155324 1101 3 0.2760180341752658 1101 4 0.1690856030418999 1101 5 0.11766140165330086 1101 6 0.14786856098279344 1101 7 0.15175241030304676 1101 8 0.16540569416193307 1101 9 0.3611870892081228 1101 10-11 0.28189540210228614 1101 12-13 0.32684140437079634 1101 14-15 0.40703046480527405 1101 16-17 0.21599293354505988 1101 18-19 0.30790689746418565 1101 20-21 0.48741914769743033 1101 22-23 0.3241348515371243 1101 24-25 0.32993404497508294 1101 26-27 0.34427793279875374 1101 28-29 0.21771481833562945 1101 30-31 0.2981044771714565 1101 32-33 0.32547499498575405 1101 34-35 0.21349448798810755 1101 36-37 0.25992549773143026 1101 38-39 0.27102591949122257 1101 40-41 0.14387230316494737 1101 42-43 0.27743747377574834 1101 44-45 0.35557132631613797 1101 46-47 0.35998237177792447 1101 48-49 0.3422780631482283 1101 50-51 0.4787208485912018 1101 52-53 0.3897536170592417 1101 54-55 0.4068546476172088 1101 56-57 0.4655210365962006 1101 58-59 0.5017181774866941 1101 60-61 0.47241559421942014 1101 62-63 0.4235994829802294 1101 64-65 0.26228143067074683 1101 66-67 0.4246336713692074 1101 68-69 0.5799847495163064 1101 70-71 0.5747872158606029 1101 72-73 0.5225035437585817 1101 74-75 0.507824096713275 1101 76-77 0.3825271060928621 1101 78-79 0.42941460373015516 1101 80-81 0.5250168005038667 1101 82-83 0.40530869126625646 1101 84-85 0.5854725186740986 1101 86-87 0.6016711659282556 1101 88-89 0.77768750415445 1101 90-91 0.8228971763767952 1101 92-93 0.9621806573153613 1101 94-95 1.0282360158625146 1101 96-97 1.3004412284183644 1101 98-99 1.726405350088946 1101 100-101 2.1375324783626617 1102 1 0.2632455282072712 1102 2 0.2869057664600021 1102 3 0.23433491409934248 1102 4 0.15012729491943588 1102 5 0.05530669587209758 1102 6 0.06950840328482855 1102 7 0.0939046117692044 1102 8 0.1061097302362981 1102 9 0.31496760497473986 1102 10-11 0.22415866408089613 1102 12-13 0.25269637128351974 1102 14-15 0.2100194654358063 1102 16-17 0.3133719054511275 1102 18-19 0.3631214870736983 1102 20-21 0.3040327653881363 1102 22-23 0.320321375876496 1102 24-25 0.23564167956927662 1102 26-27 0.25859056774324785 1102 28-29 0.23562501775095512 1102 30-31 0.3031993284220178 1102 32-33 0.34457700506160904 1102 34-35 0.33146901492605707 1102 36-37 0.33105199366478644 1102 38-39 0.26235404399363915 1102 40-41 0.1539756252675062 1102 42-43 0.3686424365485905 1102 44-45 0.5250874846255371 1102 46-47 0.37484935392544827 1102 48-49 0.49747179408084463 1102 50-51 0.43884813428820024 1102 52-53 0.310511344323281 1102 54-55 0.4167183337753215 1102 56-57 0.4972473229874268 1102 58-59 0.5062374247909887 1102 60-61 0.5099237943862889 1102 62-63 0.45918860525984684 1102 64-65 0.4948246288313598 1102 66-67 0.5128320389787078 1102 68-69 0.33200254785908356 1102 70-71 0.44406501203788906 1102 72-73 0.44696328810444896 1102 74-75 0.5008766446563868 1102 76-77 0.4482442783568743 1102 78-79 0.5164804725657 1102 80-81 0.599403857933698 1102 82-83 0.41050067070692364 1102 84-85 0.521141413450831 1102 86-87 0.6636823404469148 1102 88-89 0.8222510323598371 1102 90-91 0.8094540775735446 1102 92-93 1.0327594139471437 1102 94-95 0.9042805419724864 1102 96-97 0.9889678415424541 1102 98-99 1.4584760434886874 1102 100-101 2.042178469993715 1103 1 0.2611936841957032 1103 2 0.24596663017378972 1103 3 0.169786526173489 1103 4 0.10691979815355523 1103 5 0.08370150664215714 1103 6 0.09597832034656051 1103 7 0.11746284963668785 1103 8 0.0643255566555112 1103 9 0.2045699078376586 1103 10-11 0.1635550815900686 1103 12-13 0.20827973129199506 1103 14-15 0.23419979873098384 1103 16-17 0.2609192333808963 1103 18-19 0.20025885119598996 1103 20-21 0.1261829842021669 1103 22-23 0.3220914758868503 1103 24-25 0.16060957204626192 1103 26-27 0.24097777084114824 1103 28-29 0.1048177228594156 1103 30-31 0.13072195945536436 1103 32-33 0.24906286801602562 1103 34-35 0.18670984276665337 1103 36-37 0.15183691259182552 1103 38-39 0.04932773568509674 1103 40-41 0.029576984180934573 1103 42-43 0.12369669417358153 1103 44-45 0.018317256107202695 1103 46-47 0.21703902858219948 1103 48-49 0.30326703648193387 1103 50-51 0.2519293894917993 1103 52-53 0.19006997005120496 1103 54-55 0.2890291096753259 1103 56-57 0.13229148689506331 1103 58-59 -9.327361243620658E-4 1103 60-61 0.045682112162367616 1103 62-63 0.296349918595773 1103 64-65 0.2382654649276148 1103 66-67 0.208863167504596 1103 68-69 -0.0011045335534518586 1103 70-71 0.20158934872255685 1103 72-73 0.382342468410485 1103 74-75 0.42083194724593653 1103 76-77 0.3605897669870366 1103 78-79 0.39578831303200346 1103 80-81 0.6220253505421489 1103 82-83 0.5354783765202349 1103 84-85 0.6247844587939007 1103 86-87 0.6337631524927048 1103 88-89 0.5516270599368696 1103 90-91 0.7893262238202929 1103 92-93 1.0512415867755749 1103 94-95 1.0126495654754244 1103 96-97 1.242571711423846 1103 98-99 1.4052702367973957 1103 100-101 2.1926293772210848 1104 1 0.1496947126489232 1104 2 0.2818869458828388 1104 3 0.14023202827750936 1104 4 0.15263670520801753 1104 5 -0.009937971631039488 1104 6 0.03500401181682378 1104 7 0.1359372341029541 1104 8 0.17135439773943517 1104 9 0.25285970033232985 1104 10-11 0.15954134914990448 1104 12-13 0.3486813148217891 1104 14-15 0.23762297861021864 1104 16-17 0.1910381538827508 1104 18-19 0.1931089400222561 1104 20-21 0.2325145721635451 1104 22-23 0.3818019279467606 1104 24-25 0.2136843395441801 1104 26-27 0.27301967690259943 1104 28-29 0.2742072009379015 1104 30-31 0.2501880360757198 1104 32-33 0.35085053078306316 1104 34-35 0.3989094164316995 1104 36-37 0.3599102119834825 1104 38-39 0.20714701764482868 1104 40-41 0.13233196152847881 1104 42-43 0.1308758117054225 1104 44-45 0.17784783594297693 1104 46-47 0.348814222181403 1104 48-49 0.37513804251246796 1104 50-51 0.36795729363575447 1104 52-53 0.3719918963935456 1104 54-55 0.19934066752689006 1104 56-57 0.41130002060348403 1104 58-59 0.3760617660707908 1104 60-61 0.3926088633950755 1104 62-63 0.28823502935018297 1104 64-65 0.33171296007352424 1104 66-67 0.3255672961932632 1104 68-69 0.27052199578881897 1104 70-71 0.300087596730485 1104 72-73 0.2804011801998101 1104 74-75 0.38763950538411507 1104 76-77 0.2079682432251282 1104 78-79 0.37720820154939005 1104 80-81 0.5780738704807469 1104 82-83 0.5801995414722967 1104 84-85 0.6977411625098036 1104 86-87 0.6985004082009887 1104 88-89 0.7742585605907024 1104 90-91 0.8439584690415494 1104 92-93 1.099886139166717 1104 94-95 1.379500115372732 1104 96-97 1.4635600623705614 1104 98-99 1.9572213383443966 1104 100-101 2.597385496342522 1105 1 0.24637075262197783 1105 2 0.2805567970530234 1105 3 0.20121634648042175 1105 4 0.15177979404194986 1105 5 -0.004122756213092771 1105 6 0.06269628226831969 1105 7 0.1901731751519833 1105 8 0.13781988124470246 1105 9 0.22535825802657428 1105 10-11 0.2306335074444732 1105 12-13 0.1627267504788179 1105 14-15 0.22275916051421518 1105 16-17 0.36716607732555673 1105 18-19 0.4012526219161785 1105 20-21 0.3114518651472835 1105 22-23 0.25350792350082685 1105 24-25 0.25679516107347666 1105 26-27 0.1891975061416673 1105 28-29 0.14120442122136723 1105 30-31 0.3075526711421048 1105 32-33 0.3467354206635207 1105 34-35 0.35316653819458566 1105 36-37 0.24930069155518453 1105 38-39 0.13777412815167622 1105 40-41 0.11549793360221372 1105 42-43 0.12618720854324295 1105 44-45 0.17652938693604625 1105 46-47 0.312245417136765 1105 48-49 0.2990678112997571 1105 50-51 0.40207559094842793 1105 52-53 0.31940905963028143 1105 54-55 0.35540137750442113 1105 56-57 0.3992828904490082 1105 58-59 0.31378401227541275 1105 60-61 0.28532915290517025 1105 62-63 0.49395281348441955 1105 64-65 0.4087211943281872 1105 66-67 0.460687542131609 1105 68-69 0.4276252003299632 1105 70-71 0.5419467389317489 1105 72-73 0.3848699073801143 1105 74-75 0.42749394344969005 1105 76-77 0.3492181877518341 1105 78-79 0.5258796664882475 1105 80-81 0.552561196345021 1105 82-83 0.6057446927025936 1105 84-85 0.5991709615552594 1105 86-87 0.831047298267034 1105 88-89 0.8099769095439733 1105 90-91 0.9047297697794434 1105 92-93 1.2708068345396377 1105 94-95 1.3187606865661543 1105 96-97 1.5218152960240943 1105 98-99 1.8900768616694066 1105 100-101 2.4782034349951054 1106 1 0.004790407143978825 1106 2 0.13134616231018725 1106 3 0.04045913556409175 1106 4 0.10126165986331159 1106 5 0.0975586694271513 1106 6 0.09506181066165453 1106 7 -0.05874283224372334 1106 8 0.022131337341498636 1106 9 0.1511813201219283 1106 10-11 0.178305371671577 1106 12-13 -0.06527402801649629 1106 14-15 0.13126732746833625 1106 16-17 0.2265778992737424 1106 18-19 0.3035684384148922 1106 20-21 0.20875818855824235 1106 22-23 0.18949614668880344 1106 24-25 0.20206135056668728 1106 26-27 0.0054881416298755425 1106 28-29 0.1359868462756637 1106 30-31 0.11355888668177982 1106 32-33 0.12590363155293005 1106 34-35 -0.18400433531441962 1106 36-37 -0.00522938507276649 1106 38-39 -0.022744979146096966 1106 40-41 -0.03983693325645277 1106 42-43 -0.11346387648459455 1106 44-45 -0.08156831008434295 1106 46-47 0.02992036635858142 1106 48-49 -0.0485144017387924 1106 50-51 -0.06453544919170184 1106 52-53 -0.020109722604289004 1106 54-55 -0.17977418768110454 1106 56-57 -0.29426822832742516 1106 58-59 -0.15867692320265547 1106 60-61 -0.27131072882979623 1106 62-63 -0.3146586697354863 1106 64-65 -0.4488332123486529 1106 66-67 -0.3039571762125135 1106 68-69 -0.43840176128152564 1106 70-71 -0.09135940227818651 1106 72-73 0.007065926701699254 1106 74-75 -0.004027248807592088 1106 76-77 -0.07239251076136455 1106 78-79 0.2359357140095888 1106 80-81 0.19829510416372287 1106 82-83 0.27361561155480985 1106 84-85 0.21981847916974928 1106 86-87 0.3867304175486659 1106 88-89 0.620061115406294 1106 90-91 0.733906366057159 1106 92-93 0.9765772239736386 1106 94-95 0.8472227649415878 1106 96-97 1.2645335697521531 1106 98-99 1.4409121912772846 1106 100-101 1.8292534719529314 1107 1 0.18236105710389694 1107 2 0.2319003891522442 1107 3 0.15801715361999413 1107 4 0.15270792252807297 1107 5 -0.09559160259408372 1107 6 0.11708797463658271 1107 7 0.1641416934274602 1107 8 0.17533136493786117 1107 9 0.2880362552704909 1107 10-11 0.20176001672985677 1107 12-13 0.2541195173926525 1107 14-15 0.3698027247244724 1107 16-17 0.29122051823375017 1107 18-19 0.3623961106621536 1107 20-21 0.36654836918297207 1107 22-23 0.437606699667235 1107 24-25 0.4100224889821291 1107 26-27 0.20335833526431202 1107 28-29 0.25004932574265837 1107 30-31 0.2278462025418051 1107 32-33 0.27032374666916326 1107 34-35 0.4975998179373491 1107 36-37 0.45376743806086495 1107 38-39 0.4278634958923391 1107 40-41 0.43359602894967963 1107 42-43 0.5331756510263475 1107 44-45 0.4733196946990219 1107 46-47 0.6087570402747602 1107 48-49 0.6019496030840017 1107 50-51 0.6177676475138227 1107 52-53 0.5204756076321146 1107 54-55 0.6178884583496114 1107 56-57 0.6576067223933606 1107 58-59 0.6487692885402581 1107 60-61 0.6829702792914176 1107 62-63 0.5851700439457304 1107 64-65 0.6492077748124672 1107 66-67 0.7959592955275028 1107 68-69 0.9249843610360458 1107 70-71 1.059586306816783 1107 72-73 0.9553369245728405 1107 74-75 1.045153812863692 1107 76-77 1.0201041546787941 1107 78-79 1.127499072289151 1107 80-81 1.0751307547149942 1107 82-83 1.0773180406913028 1107 84-85 1.2059143502290546 1107 86-87 1.3839215026111518 1107 88-89 1.1670272819437386 1107 90-91 1.173465005031101 1107 92-93 1.4234540308779842 1107 94-95 1.5460818280452564 1107 96-97 1.970080848225681 1107 98-99 2.4240964823794258 1107 100-101 2.9136788188785623 1108 1 0.1084619353082843 1108 2 0.1416730552808403 1108 3 0.10671730222934173 1108 4 0.05613546586178586 1108 5 -0.04763426052354447 1108 6 0.11102669890218664 1108 7 0.0676148764859974 1108 8 0.13758250833306818 1108 9 0.23335506075878953 1108 10-11 0.20335560553035492 1108 12-13 0.14428291561446116 1108 14-15 0.24231315780542673 1108 16-17 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-0.9393216944196929 2316 86-87 -0.8450682461872923 2316 88-89 -0.7895469620432358 2316 90-91 -0.9067720474604961 2316 92-93 -0.9855290884087253 2316 94-95 -0.9466147471214974 2316 96-97 -1.1772464703364278 2316 98-99 -1.2539003448789252 2316 100-101 -2.1057367173461863 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 7.0 4 9.0 5 34.0 6 73.0 7 174.0 8 371.0 9 808.0 10 1390.0 11 2380.0 12 3884.0 13 5654.0 14 7412.0 15 10971.0 16 15650.0 17 22673.0 18 20424.0 19 10753.0 20 8465.0 21 8378.0 22 8751.0 23 9626.0 24 11043.0 25 12942.0 26 15487.0 27 19414.0 28 24212.0 29 30744.0 30 38848.0 31 48968.0 32 62896.0 33 83161.0 34 114892.0 35 165977.0 36 251941.0 37 379226.0 38 288519.0 39 22397.0 40 5.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.27371458416771 20.11146164890166 15.598658431761066 21.01616533516956 2 15.465119477735941 21.03319694175391 40.72520676756639 22.77647681294376 3 19.42178242392282 25.780232605098675 30.48922455797598 24.30876041300252 4 12.544533089541432 17.578945088878886 37.527310994456734 32.349210827122945 5 12.972221783184382 38.36458990060769 34.04071110080836 14.622477215399574 6 30.63455153196169 37.30466749504407 17.30590830529317 14.754872667701067 7 27.745512159062507 34.34656415545011 20.261377849547074 17.6465458359403 8 26.567444767848496 35.56542611004231 19.284825066239954 18.582304055869237 9 26.911008737762366 16.761473544111098 20.596572203158097 35.73094551496844 10-11 24.861008767026753 27.82121914289276 26.492293807478923 20.82547828260156 12-13 25.88460534433088 25.255106520214788 28.000055018958133 20.860233116496204 14-15 22.840858476811775 26.023741616483107 27.080742215232583 24.05465769147253 16-17 20.91259837957205 29.738622150452926 27.746448619627863 21.602330850347162 18-19 20.886816449632175 27.182318922180716 31.661936565331878 20.26892806285523 20-21 21.833503066910147 26.086967973029918 32.05111321814862 20.028415741911317 22-23 21.517322230000072 26.115942351318893 31.865622197926204 20.501113220754835 24-25 21.124044732589507 26.284903072951206 32.1675710381826 20.423481156276686 26-27 20.952669141104096 26.655737234762032 31.918896669752154 20.472696954381718 28-29 20.692966155016755 26.562063768455246 32.205118450125106 20.539851626402893 30-31 20.82929727167109 26.43183860045477 32.215732007456594 20.523132120417547 32-33 20.78005994518194 26.57689716668003 31.871323294866265 20.771719593271765 34-35 21.144694277816246 26.52103144107819 31.87480575759367 20.45946852351189 36-37 21.093510855041174 26.447782666231994 31.714027184279637 20.7446792944472 38-39 20.98657876423493 26.6965885326892 31.78604685463381 20.53078584844205 40-41 21.112971676164914 26.543857667358672 31.848087367088446 20.49508328938797 42-43 20.995656172057966 26.595336809098097 31.547218715044067 20.86178830379987 44-45 21.059008136086447 26.862898076217355 31.18419535503854 20.893898432657657 46-47 21.257128074443933 27.131867109222323 30.5713111794077 21.039693636926042 48-49 21.363357819826156 27.354539873027655 30.166028605358548 21.116073701787645 50-51 21.4974765314203 27.520849416555805 29.760248536634045 21.221425515389853 52-53 21.64223088448752 27.736222315868336 29.596941283888185 21.024605515755958 54-55 21.400933300010944 27.711799578709805 29.648341499074366 21.238925622204885 56-57 21.400611391691605 28.02516269539103 29.42862443892843 21.14560147398893 58-59 21.498822693482992 27.561936623860667 29.711962288733034 21.227278393923307 60-61 21.56674534886375 27.629156933817406 29.866566075194275 20.937531642124572 62-63 21.5166154442247 27.575222658131608 29.969371886634427 20.938790011009264 64-65 21.45360920681205 27.363845949895847 29.936449444883735 21.246095398408368 66-67 21.257098810051264 27.858999473826216 29.565757382967305 21.31814433315521 68-69 21.374068587542382 27.670566049441607 29.717932224837156 21.237433138178854 70-71 21.38957871565604 27.79894894007296 29.55138856616767 21.26008377810333 72-73 21.556229092402106 27.805938277244664 29.254819630220663 21.383013000132568 74-75 21.437467809149226 28.03694339092569 29.072552324764718 21.45303647516037 76-77 21.52697503922892 27.928882843515684 29.321633819336856 21.22250829791854 78-79 21.47661101944853 27.85232719229808 29.211511909729882 21.459549878523507 80-81 21.33229152034462 28.183792199278923 29.103104368591094 21.380811911785365 82-83 21.520447231332415 28.124647911848395 29.040450228014116 21.314454628805077 84-85 21.31441481251701 27.995762501060838 28.959325277793223 21.730497408628928 86-87 21.37784369419646 27.88867356799084 29.078651567020437 21.654831170792264 88-89 21.563555530063017 27.81147410013456 29.107506258190373 21.517464111612053 90-91 21.26219905866845 28.005169655666517 29.182682824181956 21.54994846148308 92-93 21.310404589260646 27.79649291005235 29.195284430502475 21.69781807018453 94-95 21.226137082609284 27.674809386378364 29.258276409212197 21.84077712180016 96-97 21.409273651921122 27.602965302380188 29.119621716754622 21.868139328944064 98-99 21.246038692851315 27.57303890970078 28.969801151087303 22.2111212463606 100-101 20.973996306970424 27.590513321745174 29.47929102495473 21.95619934632967 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 754.0 1 517.0 2 436.5 3 661.5 4 550.5 5 450.5 6 940.5 7 2310.5 8 3896.0 9 4633.0 10 4143.0 11 3417.5 12 3335.5 13 3543.5 14 3855.5 15 4119.5 16 4333.5 17 4444.0 18 4596.0 19 4823.0 20 5106.0 21 5664.5 22 6518.5 23 7621.5 24 8951.0 25 10655.0 26 12760.5 27 15422.5 28 17481.5 29 19580.0 30 22915.0 31 25666.5 32 28918.0 33 33433.0 34 38135.5 35 42024.0 36 46870.0 37 51528.5 38 54075.5 39 55594.0 40 56281.0 41 57896.0 42 59552.0 43 61474.0 44 63580.5 45 64522.0 46 63470.5 47 62405.5 48 65214.5 49 64439.5 50 60120.5 51 56864.0 52 52481.5 53 49624.0 54 46808.5 55 42303.0 56 37496.0 57 32964.0 58 29042.5 59 25577.0 60 21921.5 61 17754.5 62 13768.5 63 10264.0 64 7462.0 65 5552.5 66 4114.0 67 3095.5 68 2222.0 69 1515.0 70 1103.0 71 833.5 72 663.5 73 534.5 74 443.5 75 363.0 76 233.5 77 124.0 78 68.0 79 45.0 80 24.5 81 11.5 82 9.0 83 6.0 84 1.5 85 1.0 86 1.0 87 0.5 88 1.0 89 0.5 90 0.5 91 1.0 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04149690880220255 2 0.0 3 0.0 4 0.0 5 0.01638805989367661 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0034531983347389997 14-15 0.0 16-17 0.0 18-19 0.0 20-21 5.852878533455932E-5 22-23 2.926439266727966E-4 24-25 4.6823028267647455E-4 26-27 0.0014339552406967032 28-29 0.002107036272044135 30-31 3.5117271200735587E-4 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.00936460565352949 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 5.852878533455932E-5 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 8.779317800183897E-5 74-75 5.852878533455932E-5 76-77 0.0 78-79 0.0 80-81 5.852878533455932E-5 82-83 2.633795340055169E-4 84-85 3.5117271200735587E-4 86-87 0.0 88-89 0.0 90-91 0.007608742093492711 92-93 0.0029557036593952455 94-95 0.0 96-97 0.0 98-99 1.463219633363983E-4 100-101 0.012144722956921058 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1708561.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 53.1433831567315 #Duplication Level Percentage of deduplicated Percentage of total 1 75.57733406574452 40.16435222220158 2 13.673095120592412 14.53269065864157 3 4.607013244952579 7.344968102539555 4 1.997957993335303 4.247129886834896 5 1.088657350292 2.8927467346479916 6 0.6301676559039374 2.0093544714409366 7 0.45163816453422734 1.6801106018231773 8 0.3082982285789988 1.3107208710332259 9 0.2313105138955296 1.1063360941317535 >10 1.2720861343981658 12.650880465588369 >50 0.10500933648923713 3.8729334004831086 >100 0.05454972610481849 5.065121641224936 >500 0.002217280906378084 0.7929036992386004 >1k 3.325921359567126E-4 0.35974661818627535 >5k 1.108640453189042E-4 0.4680948053537435 >10k+ 2.217280906378084E-4 1.5019097266302868 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13796 0.8074631224755803 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11696 0.6845526732730057 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7945 0.4650111994830738 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2791 0.16335383986875507 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2280 0.13344563056279524 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 5.852878533455932E-5 0.0 14-15 0.0 0.0 0.0 5.852878533455932E-5 0.0 16-17 2.926439266727966E-5 0.0 0.0 5.852878533455932E-5 0.0 18-19 5.852878533455932E-5 0.0 0.0 1.1705757066911864E-4 0.0 20-21 5.852878533455932E-5 0.0 0.0 2.3411514133823727E-4 0.0 22-23 5.852878533455932E-5 0.0 0.0 4.097014973419152E-4 0.0 24-25 5.852878533455932E-5 0.0 0.0 5.852878533455932E-4 0.0 26-27 5.852878533455932E-5 0.0 0.0 7.901386020165508E-4 0.0 28-29 5.852878533455932E-5 0.0 0.0 0.0016388059893676608 0.0 30-31 5.852878533455932E-5 0.0 0.0 0.0030142324447298046 0.0 32-33 5.852878533455932E-5 0.0 0.0 0.005911407318790491 0.0 34-35 5.852878533455932E-5 0.0 0.0 0.008223294339505584 0.0 36-37 5.852878533455932E-5 0.0 0.0 0.010476652574886117 0.0 38-39 5.852878533455932E-5 0.0 0.0 0.012964125951604889 0.0 40-41 5.852878533455932E-5 0.0 0.0 0.0169733477470222 0.0 42-43 5.852878533455932E-5 0.0 0.0 0.02203608767846158 0.0 44-45 5.852878533455932E-5 0.0 0.0 0.026484275363888088 0.0 46-47 5.852878533455932E-5 0.0 0.0 0.031868923614667546 0.0 48-49 5.852878533455932E-5 0.0 0.0 0.03751695139945252 0.0 50-51 5.852878533455932E-5 0.0 0.0 0.04650111994830738 0.0 52-53 5.852878533455932E-5 0.0 0.0 0.06590341228671379 0.0 54-55 5.852878533455932E-5 0.0 0.0 0.0876175916458353 0.0 56-57 5.852878533455932E-5 0.0 0.0 0.10906839147095129 0.0 58-59 5.852878533455932E-5 0.0 0.0 0.14626343455106372 0.0 60-61 5.852878533455932E-5 0.0 0.0 0.19396439459872955 0.0 62-63 5.852878533455932E-5 0.0 0.0 0.22691610074208646 0.0 64-65 5.852878533455932E-5 0.0 0.0 0.2554781479853514 0.0 66-67 5.852878533455932E-5 0.0 0.0 0.327848991051534 0.0 68-69 5.852878533455932E-5 0.0 0.0 0.40317553777711185 0.0 70-71 1.1705757066911864E-4 0.0 0.0 0.4398438217892133 0.0 72-73 1.7558635600367796E-4 0.0 0.0 0.508146914274644 0.0 74-75 1.7558635600367796E-4 0.0 0.0 0.6140254869448618 0.0 76-77 1.7558635600367796E-4 0.0 0.0 0.7228304988818075 0.0 78-79 2.048507486709576E-4 0.0 0.0 0.8310502229654078 0.0 80-81 2.3411514133823727E-4 0.0 0.0 0.9674515571875981 0.0 82-83 2.3411514133823727E-4 0.0 0.0 1.1162024651153808 0.0 84-85 2.3411514133823727E-4 0.0 0.0 1.2684065713779022 0.0 86-87 2.3411514133823727E-4 0.0 0.0 1.4731109980855233 0.0 88-89 2.3411514133823727E-4 0.0 0.0 1.709772141585814 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 6390 0.0 47.44778 1 GTATCAA 11010 0.0 39.148582 1 CCGTCCG 75 6.92297E-6 38.00103 9 ATCAACG 14160 0.0 30.194149 3 TATCAAC 14335 0.0 29.92496 2 TCAACGC 14355 0.0 29.750895 4 CAACGCA 14655 0.0 29.141867 5 AACGCAG 15085 0.0 28.307861 6 GTACATG 10150 0.0 25.7041 1 GTATAGG 510 0.0 25.158785 1 ACCGTCC 170 1.1232078E-7 25.147741 8 TACCTGG 1250 0.0 25.082882 2 TACATGG 10380 0.0 24.668056 2 CGCAGAG 17240 0.0 24.522125 8 ACGCAGA 17330 0.0 24.504414 7 ACATGGG 10510 0.0 23.73013 3 GCAGAGT 18035 0.0 23.046091 9 GAGTACT 10880 0.0 22.72432 12-13 TACACCG 195 4.219255E-7 21.925596 5 AGTACTT 11185 0.0 21.892317 12-13 >>END_MODULE