FastQCFastQC Report
Thu 26 May 2016
SRR937619_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937619_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2229554
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT88480.396850670582547No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT66460.2980865231342233No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43830.1965864024822902No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN29420.13195464204948615No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA57400.045.2437481
GTATCAA98000.039.701331
ATCAACG124200.031.1215423
TCAACGC126600.030.5349924
TATCAAC127650.030.4294012
CAACGCA128900.029.9901495
AACGCAG131450.029.3852276
ACGCAGA149800.025.6258967
CGCAGAG151750.025.2948958
GTACATG114550.025.1625921
GAGTACT75350.024.14795712-13
TACATGG122100.023.3240132
GCAGAGT161550.023.3188299
GTACTTT80100.022.77373714-15
ACATGGG120500.022.5289633
AGTACTT80650.021.38292712-13
AGAGTAC143250.021.09246610-11
CATGGGG57000.020.3542924
GGACCGA6250.019.76486
CAGAGTA156850.019.7482210-11
Produced by FastQC (version 0.11.5)