FastQCFastQC Report
Thu 26 May 2016
SRR937619_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937619_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2229554
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT109530.4912641721169346No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT91680.41120331689656314No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61040.27377672843985834No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22370.10033396813891927No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA62750.046.580441
GTATCAA106400.039.486881
ATCAACG136300.030.5416133
TATCAAC138350.030.1921082
TCAACGC139000.029.9825484
CAACGCA142000.029.3156495
AACGCAG146250.028.4821746
ACGCAGA164900.025.0891747
CGCAGAG166000.024.8656928
GTACATG108050.024.632331
TACATGG113350.023.3935642
GAGTACT95900.022.75913612-13
GCAGAGT179600.022.5331639
ACATGGG114300.022.4507713
GTACTTT100650.021.66049214-15
AGTACTT100500.021.12663712-13
GGACCGA7450.021.0390956
AGAGTAC159800.020.64354910-11
CATGGGA62600.020.1166324
GTACTAG3153.1832315E-1019.6143991
Produced by FastQC (version 0.11.5)