##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937619_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2229554 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.12658226712607 33.0 31.0 34.0 31.0 34.0 2 32.400141014750034 34.0 31.0 34.0 31.0 34.0 3 32.49418134748026 34.0 31.0 34.0 31.0 34.0 4 36.14470382865811 37.0 35.0 37.0 35.0 37.0 5 36.051661453366904 37.0 35.0 37.0 35.0 37.0 6 36.093610650381194 37.0 35.0 37.0 35.0 37.0 7 36.037680630296464 37.0 35.0 37.0 35.0 37.0 8 36.00804779790039 37.0 35.0 37.0 35.0 37.0 9 37.70631166592063 39.0 37.0 39.0 35.0 39.0 10-11 37.61098340744382 39.0 37.0 39.0 35.0 39.0 12-13 37.50349509363756 39.0 37.0 39.0 35.0 39.0 14-15 38.71695123778119 40.0 38.0 41.0 34.5 41.0 16-17 38.67063278126477 40.0 38.0 41.0 34.5 41.0 18-19 38.63938370633768 40.0 38.0 41.0 34.0 41.0 20-21 38.528838503126636 40.0 38.0 41.0 34.0 41.0 22-23 38.47351465808857 40.0 38.0 41.0 34.0 41.0 24-25 38.493054664744605 40.0 38.0 41.0 34.0 41.0 26-27 38.363102889636224 40.0 38.0 41.0 34.0 41.0 28-29 38.203027825296004 40.0 38.0 41.0 34.0 41.0 30-31 38.25696394884358 40.0 38.0 41.0 34.0 41.0 32-33 38.18413884570636 40.0 38.0 41.0 34.0 41.0 34-35 38.0386763451345 40.0 37.0 41.0 33.0 41.0 36-37 37.884113594019254 40.0 37.0 41.0 33.0 41.0 38-39 37.74739409765361 40.0 37.0 41.0 33.0 41.0 40-41 37.5573715639989 40.0 37.0 41.0 33.0 41.0 42-43 37.322439600027636 40.0 36.5 41.0 32.5 41.0 44-45 37.01234394861035 40.0 36.0 41.0 31.5 41.0 46-47 36.622093925511564 39.0 36.0 41.0 31.0 41.0 48-49 36.28358250125361 39.0 35.5 41.0 30.0 41.0 50-51 35.99746541236499 39.0 35.0 40.0 29.5 41.0 52-53 35.73535738537842 39.0 35.0 40.0 28.5 41.0 54-55 35.475356506278835 38.0 35.0 40.0 28.0 41.0 56-57 35.176340200775584 38.0 34.5 40.0 27.5 41.0 58-59 34.880669631684185 38.0 34.0 40.0 27.0 41.0 60-61 34.519318437678564 37.5 34.0 40.0 26.0 41.0 62-63 34.1224208967354 37.0 33.5 39.5 26.0 41.0 64-65 33.68352908249811 36.0 33.0 39.0 25.5 40.5 66-67 33.2322060824721 36.0 32.5 39.0 24.5 40.0 68-69 32.76422818195927 35.0 32.0 38.5 23.0 40.0 70-71 32.355568647361764 35.0 31.5 37.5 22.5 40.0 72-73 31.870776173171855 35.0 31.0 37.0 21.5 39.0 74-75 31.38984433658032 34.5 31.0 36.5 20.0 39.0 76-77 30.197760628358857 33.5 29.5 35.0 19.5 37.0 78-79 30.812239129440236 34.0 30.5 35.5 20.0 37.0 80-81 30.63958890432795 34.0 31.0 35.0 18.5 37.0 82-83 30.281921182442765 34.0 30.0 35.0 17.5 36.5 84-85 29.914843731078054 34.0 30.0 35.0 13.5 36.0 86-87 29.536882040085146 34.0 29.5 35.0 7.5 36.0 88-89 29.09214443785618 34.0 29.0 35.0 4.5 35.0 90-91 28.529526533109312 33.0 29.0 35.0 2.0 35.0 92-93 28.0594910461913 33.0 27.5 35.0 2.0 35.0 94-95 27.5584399391089 33.0 27.0 35.0 2.0 35.0 96-97 26.941854738660737 33.0 25.5 34.0 2.0 35.0 98-99 25.96497124537015 32.0 24.5 34.0 2.0 35.0 100-101 23.07295853789592 29.5 10.5 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.24678667279025035 1101 2 0.21918243572003604 1101 3 0.22989522845827537 1101 4 0.08071105489389652 1101 5 0.07784629044625291 1101 6 0.06356121986848962 1101 7 0.09644860708866076 1101 8 0.00905413840852276 1101 9 0.10096784554867355 1101 10-11 0.1268409837628255 1101 12-13 0.2042992663952461 1101 14-15 0.26377977162331234 1101 16-17 0.16346184069778502 1101 18-19 0.18910834073556515 1101 20-21 0.29526799501377354 1101 22-23 0.21054557476491453 1101 24-25 0.14186686395319015 1101 26-27 0.04665468479914381 1101 28-29 -0.053970871267132736 1101 30-31 0.043579821136709995 1101 32-33 0.21867569458905933 1101 34-35 0.23338705332490406 1101 36-37 0.09035287709149031 1101 38-39 0.11159812594499385 1101 40-41 0.1675966039279615 1101 42-43 0.3435859319143262 1101 44-45 0.20770764422967858 1101 46-47 0.282194068056576 1101 48-49 0.2517262099144446 1101 50-51 0.2558177665500807 1101 52-53 0.3893673762440457 1101 54-55 0.24753186061739285 1101 56-57 0.13696092205214683 1101 58-59 0.201337780750805 1101 60-61 0.25220602179224016 1101 62-63 0.10802645172122993 1101 64-65 0.18431913565750335 1101 66-67 0.2759788152457503 1101 68-69 0.2542799264177589 1101 70-71 0.24640887875617778 1101 72-73 0.15368597863926325 1101 74-75 0.041740401407622585 1101 76-77 0.07550329462968719 1101 78-79 0.18491983321978012 1101 80-81 0.08452602523863817 1101 82-83 0.2994568627982517 1101 84-85 0.3042216104755475 1101 86-87 0.36373707014369216 1101 88-89 0.350461420898025 1101 90-91 0.2719607462448721 1101 92-93 0.22714746292065158 1101 94-95 -0.12147500515865417 1101 96-97 -0.08865289163484391 1101 98-99 -0.14542224593362008 1101 100-101 -0.038160238997672735 1102 1 0.29485602606693817 1102 2 0.25859338544920973 1102 3 0.20636432739603805 1102 4 0.11788764462593093 1102 5 0.031586433675308 1102 6 0.09867988999198474 1102 7 0.14961694280280113 1102 8 0.09785474504905523 1102 9 0.07573335358322453 1102 10-11 0.16399233817551817 1102 12-13 0.29182921265228146 1102 14-15 0.30822754630251836 1102 16-17 0.25897628336748113 1102 18-19 0.3137625444640193 1102 20-21 0.314643445681412 1102 22-23 0.2599420576233058 1102 24-25 0.19561209561548765 1102 26-27 0.24348428061620808 1102 28-29 0.10450300417878111 1102 30-31 0.1287088956582565 1102 32-33 0.2834047024231836 1102 34-35 0.20645900252992533 1102 36-37 0.1381623272635224 1102 38-39 0.2022314993652543 1102 40-41 0.23987800504253443 1102 42-43 0.13678910891692198 1102 44-45 0.05822705742991019 1102 46-47 0.15410310341788147 1102 48-49 0.20995617151952217 1102 50-51 0.2671464598941995 1102 52-53 0.1634799307326844 1102 54-55 0.13039954025460077 1102 56-57 0.14759243798742716 1102 58-59 0.22159119300036423 1102 60-61 0.04540950906515917 1102 62-63 0.08849359057795425 1102 64-65 -0.041179541143009146 1102 66-67 -0.04245026957677567 1102 68-69 0.0939822820313907 1102 70-71 -0.1045049914440952 1102 72-73 -0.015248453795653205 1102 74-75 -0.07753816218145104 1102 76-77 -0.12388148069529947 1102 78-79 -0.01853661951343355 1102 80-81 0.19908610122590886 1102 82-83 0.19075438018063195 1102 84-85 0.0801290787648874 1102 86-87 0.02548119476148969 1102 88-89 0.027718958088058798 1102 90-91 0.2289872727024509 1102 92-93 0.052208679254771795 1102 94-95 -0.2828150543964867 1102 96-97 -0.29569886169913673 1102 98-99 -0.16628988999316974 1102 100-101 -0.2580160294899194 1103 1 0.20089931094482694 1103 2 0.17772366799452044 1103 3 0.09676510872361632 1103 4 0.0837932958911054 1103 5 0.0662716035658022 1103 6 0.07794711223924367 1103 7 0.1166422230889097 1103 8 0.10095660596620348 1103 9 0.10215619967820544 1103 10-11 0.13573641112069623 1103 12-13 0.24563260140941878 1103 14-15 0.12064781136845681 1103 16-17 0.06509212977710632 1103 18-19 0.13762735230059064 1103 20-21 0.06652159096159949 1103 22-23 0.045238287733688765 1103 24-25 0.029528739384424796 1103 26-27 0.01595203470220241 1103 28-29 0.0869383877259935 1103 30-31 0.09378295069335962 1103 32-33 0.08749724060921693 1103 34-35 0.23820203062094691 1103 36-37 0.17510799125837195 1103 38-39 0.16446769861654786 1103 40-41 0.1496677101982513 1103 42-43 0.2168621250798637 1103 44-45 0.32269330245937056 1103 46-47 0.1962722405797095 1103 48-49 0.031754002609680754 1103 50-51 0.11570900777503823 1103 52-53 0.13996911709039495 1103 54-55 0.030093940354781523 1103 56-57 0.07514675744241117 1103 58-59 0.07714279822734937 1103 60-61 0.13612697691296916 1103 62-63 -0.03524186670725271 1103 64-65 -0.010045678975217243 1103 66-67 0.11656287365159557 1103 68-69 -0.04204865602031305 1103 70-71 -0.05658297505105736 1103 72-73 -7.393378419671137E-4 1103 74-75 0.07557424697705173 1103 76-77 0.12561124660135548 1103 78-79 0.1538526508712934 1103 80-81 -0.0782240733006283 1103 82-83 0.02345279180291726 1103 84-85 -0.16563377842468796 1103 86-87 -0.30361306823148837 1103 88-89 -0.3396899471799628 1103 90-91 -0.15996227799032425 1103 92-93 0.045585819361338764 1103 94-95 -0.29054149163923526 1103 96-97 -0.4485390903387483 1103 98-99 -0.38533678360587587 1103 100-101 -0.25032578328319843 1104 1 0.3385925590970018 1104 2 0.19015236549433467 1104 3 0.2535998243031301 1104 4 0.1586132255341397 1104 5 0.1164977988237581 1104 6 0.14213705845583036 1104 7 0.200229967624189 1104 8 0.19464889748478953 1104 9 0.14065190592635446 1104 10-11 0.21609970570980863 1104 12-13 0.2575996310579356 1104 14-15 0.2417563042559081 1104 16-17 0.2926524963302839 1104 18-19 0.23408137881073543 1104 20-21 0.16250902823487934 1104 22-23 0.09963823680257633 1104 24-25 0.19002119626157565 1104 26-27 0.17606051879398166 1104 28-29 0.12281602155731264 1104 30-31 0.19007446676911144 1104 32-33 0.1815023018877966 1104 34-35 0.22757267545649285 1104 36-37 0.13391547042415652 1104 38-39 0.045714769345025275 1104 40-41 0.027482795103672686 1104 42-43 0.14524622686835897 1104 44-45 0.06993418522610284 1104 46-47 -0.11116870593467354 1104 48-49 -0.08247544034392718 1104 50-51 -0.08030224062760283 1104 52-53 -0.030331556532111392 1104 54-55 -0.06519225182888277 1104 56-57 -0.1571901802997644 1104 58-59 -0.03671031579508366 1104 60-61 -0.06443540133509629 1104 62-63 -0.007677919060739669 1104 64-65 -0.039326457215906885 1104 66-67 0.037405318528932696 1104 68-69 0.08458707642216723 1104 70-71 -0.032954984052800285 1104 72-73 -0.01766366871153835 1104 74-75 0.024672879502009692 1104 76-77 -0.13928044731443023 1104 78-79 -0.15762739196779307 1104 80-81 0.03856909740778747 1104 82-83 0.01499334883675374 1104 84-85 -0.021628382073043184 1104 86-87 0.03176019687538556 1104 88-89 0.14325682353247515 1104 90-91 0.1443424893872951 1104 92-93 0.2040290950523307 1104 94-95 0.08499509645419323 1104 96-97 0.004075296274415052 1104 98-99 0.2982717354591493 1104 100-101 0.3456198745678343 1105 1 0.25758922780784843 1105 2 0.19294898428206153 1105 3 0.16472743948838797 1105 4 0.06479475459894246 1105 5 0.07772880415814143 1105 6 0.10592739371109872 1105 7 0.10727898780606893 1105 8 0.0968627717956494 1105 9 0.09901695481710249 1105 10-11 0.14894695639470967 1105 12-13 0.1756001487791039 1105 14-15 0.21615144877099368 1105 16-17 0.23133181429428618 1105 18-19 0.15193320578092084 1105 20-21 0.030696603543276524 1105 22-23 0.043733596110314465 1105 24-25 0.05154773409373803 1105 26-27 0.08006407504530699 1105 28-29 0.07296378780620927 1105 30-31 0.02855239736366144 1105 32-33 0.07583864807067897 1105 34-35 0.14468335992701498 1105 36-37 0.09935758272650475 1105 38-39 0.20447103669869904 1105 40-41 0.23567527027308444 1105 42-43 0.3109123768410811 1105 44-45 0.30151117005502925 1105 46-47 0.1402103429992252 1105 48-49 0.08583093348156723 1105 50-51 0.1689150142791931 1105 52-53 0.2903759032751623 1105 54-55 0.24383104254182086 1105 56-57 0.13460655867324078 1105 58-59 0.14162228307270652 1105 60-61 0.16509612264494677 1105 62-63 0.15348282411567027 1105 64-65 0.12280424272461232 1105 66-67 0.04479517181044912 1105 68-69 0.17631386140455874 1105 70-71 0.07631037577882438 1105 72-73 0.06613705178375184 1105 74-75 -0.1632206116951913 1105 76-77 -0.13037132895043158 1105 78-79 0.08370694188223382 1105 80-81 0.10181351418565043 1105 82-83 0.022847764412762928 1105 84-85 0.14173652814239546 1105 86-87 0.03931569011264813 1105 88-89 0.1168430287287947 1105 90-91 0.2565901388407603 1105 92-93 0.20029102498123663 1105 94-95 0.16030503577604094 1105 96-97 0.2793265297142682 1105 98-99 0.3377880359716343 1105 100-101 0.18299687727046532 1106 1 0.1421181387099537 1106 2 0.13230710587795613 1106 3 0.1363621630582017 1106 4 0.08809139833562085 1106 5 0.03286191988643594 1106 6 -0.04719670649046037 1106 7 -0.12502664968071997 1106 8 -0.12639830343778158 1106 9 0.050214790703549284 1106 10-11 -0.019726628789435097 1106 12-13 -0.24253627941728695 1106 14-15 0.05785479293618323 1106 16-17 0.07946882320092641 1106 18-19 0.10463629104612693 1106 20-21 0.08499519023968816 1106 22-23 0.13524820944196847 1106 24-25 0.07391330571829968 1106 26-27 0.052360445364243446 1106 28-29 0.0508803445815289 1106 30-31 0.04297494150369374 1106 32-33 0.019743368035257447 1106 34-35 -0.26467043053821016 1106 36-37 0.05347348765455706 1106 38-39 0.18807480357256168 1106 40-41 0.0859419130661081 1106 42-43 -0.17896511749093946 1106 44-45 -0.11194485445094671 1106 46-47 -0.22610374382179543 1106 48-49 -0.08928381728484425 1106 50-51 -0.03360901865676169 1106 52-53 0.00825464129498954 1106 54-55 -0.009117755750892798 1106 56-57 -0.11346330560993323 1106 58-59 -0.1671516440045977 1106 60-61 -0.049367071213076485 1106 62-63 -0.11036554275771948 1106 64-65 -0.4886520818149904 1106 66-67 -0.44617236406655536 1106 68-69 -0.09889670655427096 1106 70-71 -0.30638615229973 1106 72-73 -0.2825519948109907 1106 74-75 -0.21150727447432516 1106 76-77 -0.313087214037548 1106 78-79 -0.29688100305675746 1106 80-81 -0.3412767596434314 1106 82-83 -0.4572211707340017 1106 84-85 -0.6212036320852441 1106 86-87 -0.22809908460806483 1106 88-89 -0.1895925717135114 1106 90-91 -0.3184992058519107 1106 92-93 -0.3519963685249685 1106 94-95 -0.2807877157501366 1106 96-97 -0.05368963096825752 1106 98-99 -0.37134072926898654 1106 100-101 -0.8436061854922166 1107 1 0.22620015284629602 1107 2 0.1433106437725371 1107 3 0.1417642461746027 1107 4 -0.022048618985586188 1107 5 0.05359123920364084 1107 6 0.1111556962555369 1107 7 0.10280792009555739 1107 8 0.11574150054305221 1107 9 0.07642721168506483 1107 10-11 0.1838295521130462 1107 12-13 0.17284530778432128 1107 14-15 0.14951102539592398 1107 16-17 0.06282827379866518 1107 18-19 0.13524796838404285 1107 20-21 0.14633016764847184 1107 22-23 0.18969178472191572 1107 24-25 0.15382540440346304 1107 26-27 0.18104101132578165 1107 28-29 0.17236448247052039 1107 30-31 0.173131873669071 1107 32-33 0.2068910071954022 1107 34-35 0.29221614603448387 1107 36-37 0.24769082054427827 1107 38-39 0.28301723976728965 1107 40-41 0.26285023003911334 1107 42-43 0.3341661096987778 1107 44-45 0.28680545349572384 1107 46-47 0.14335061276648275 1107 48-49 0.22796513315669387 1107 50-51 0.2644149161017282 1107 52-53 0.24217804702013268 1107 54-55 0.33528523406210553 1107 56-57 0.3772596896889553 1107 58-59 0.2293109693642208 1107 60-61 0.38397574391478173 1107 62-63 0.2794005955528789 1107 64-65 0.2634183357479998 1107 66-67 0.19830034233390847 1107 68-69 0.283131652477266 1107 70-71 0.2650765245632556 1107 72-73 0.4539406385965954 1107 74-75 0.27988037768942675 1107 76-77 0.20179734088109313 1107 78-79 0.3532167779493456 1107 80-81 0.3006179568805436 1107 82-83 0.4413881801779027 1107 84-85 0.5304032781111552 1107 86-87 0.548872726524749 1107 88-89 0.5153204793840445 1107 90-91 0.3405668712503207 1107 92-93 0.2243609461292273 1107 94-95 0.34042428616992737 1107 96-97 0.5023934905062291 1107 98-99 0.5307168624737244 1107 100-101 0.7610904775087199 1108 1 0.12918505987369855 1108 2 0.0965982988204388 1108 3 0.10602621182507477 1108 4 0.04113593280965944 1108 5 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>>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 7.0 4 12.0 5 50.0 6 94.0 7 221.0 8 537.0 9 998.0 10 1758.0 11 2769.0 12 4629.0 13 6436.0 14 8333.0 15 11610.0 16 16322.0 17 22666.0 18 20121.0 19 12448.0 20 10924.0 21 11270.0 22 11882.0 23 12833.0 24 14845.0 25 18209.0 26 21969.0 27 27421.0 28 34777.0 29 43891.0 30 54825.0 31 68727.0 32 89122.0 33 118715.0 34 164835.0 35 239412.0 36 363342.0 37 496585.0 38 300776.0 39 16179.0 40 2.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.00239819341479 19.175947367146094 15.304924057208533 22.51673038223058 2 15.870079845565527 20.039927267964806 39.60913258884961 24.48086029762006 3 19.45806201599064 24.25251866516801 29.7768073794131 26.512611939428243 4 13.389544899807271 16.593998886772372 35.84951781814561 34.16693839527475 5 14.12705342644189 37.4418987612697 33.23581553521342 15.195232277074986 6 33.03741465781946 35.5679207590397 16.56483763120337 14.829826951937472 7 29.603095506993775 32.89864250877081 19.520585731496077 17.977676252739336 8 27.441990640280522 34.630065026458205 18.777746580706275 19.150197752554995 9 26.946779490427236 16.448581196059838 19.86733669603876 36.737302617474164 10-11 25.822159947684604 26.62954115486775 25.89627791029058 21.652020987157073 12-13 26.44354915757363 23.71691621218299 27.2667331403712 22.57280148987218 14-15 23.146557562633603 24.906932059057553 25.66226249734252 26.284247880966326 16-17 22.637061044495894 28.487042700019828 26.251775018680867 22.624121236803415 18-19 22.65466546224043 26.43804545662496 29.04789478074987 21.85939430038474 20-21 23.188449349062637 25.469219404419004 29.37309434981167 21.969236896706697 22-23 23.182779754644294 25.254262261001546 29.38193262865835 22.18102535569581 24-25 22.85266790204218 25.547851215816276 29.391337226343506 22.208143655798036 26-27 22.449943003771867 25.999272942075102 29.22342274740433 22.3273613067487 28-29 22.370581641985115 25.951735184004903 29.454172689975884 22.2235104840341 30-31 22.418878394512983 25.711218476879232 29.3304400790472 22.539463049560585 32-33 22.29277694103843 25.801954112795656 29.28940048099306 22.615868465172856 34-35 22.907563575495367 25.758111263508304 29.099900697628318 22.23442446336801 36-37 22.883567744939125 25.489156127189567 28.89755081061055 22.729725317260762 38-39 22.55599101883157 25.815230310636117 29.097321706493766 22.531456964038547 40-41 22.774034236156414 25.73670350006627 29.087613675176105 22.40164858860121 42-43 22.726062609941504 25.738855130319443 28.761269459772425 22.773812799966624 44-45 22.636343412180192 25.94494235169904 28.644361159227362 22.774353076893405 46-47 22.860042860590056 26.26330198775181 28.12703796364654 22.74961718801159 48-49 23.066070613225783 26.44654491436404 27.69840963708437 22.78897483532581 50-51 23.030144145421012 26.52557417313059 27.681724685744324 22.762556995704074 52-53 23.392194894628005 26.459176691655973 27.45072051421955 22.69790789949647 54-55 23.03743600450584 26.53179350196665 27.537959988813892 22.892810504713616 56-57 22.97952864115424 26.82016223872577 27.472557291727405 22.727751828392584 58-59 23.366915535573483 26.294945087672243 27.429297518696565 22.90884185805771 60-61 23.163511626092035 26.413354419762875 27.810023888185707 22.61311006595938 62-63 23.00078849850688 26.527368253919843 27.85492075993674 22.61692248763654 64-65 22.911588107571337 26.390245040149303 27.751011077108284 22.947155775171076 66-67 22.876593255870905 26.602203848841516 27.619761620485534 22.901441274802046 68-69 22.932030352258792 26.342891896765003 27.803497919314808 22.921579831661397 70-71 22.94167353650102 26.47549689310059 27.644968455574524 22.937861114823864 72-73 23.065958483176455 26.464754834374947 27.342665842585557 23.12662083986304 74-75 22.88062993764672 26.752076872773657 27.270992315054936 23.09630087452468 76-77 23.067505877857187 26.367829619735605 27.535933195607733 23.02873130679948 78-79 22.962641855725405 26.604962248054992 27.403574885380667 23.02882101083894 80-81 22.84820192738099 27.005692618344295 27.304451921774493 22.84165353250022 82-83 23.027097311842788 26.872624087899805 27.214488558955136 22.885790041302272 84-85 22.919212954345557 26.687501934244217 27.22420144306953 23.169083668340697 86-87 23.01267428373567 26.676007847309375 27.270790480966152 23.040527387988806 88-89 22.91610788525418 26.714872122406547 27.16529853055813 23.203721461781146 90-91 22.845358142539297 26.66170243307256 27.36553954640063 23.12739987798751 92-93 22.991814556270636 26.374805366120256 27.424364988395418 23.20901508921369 94-95 22.80619801090263 26.31701228138004 27.56145399483484 23.315335712882487 96-97 22.950868200545937 26.25933258400559 27.38720389817874 23.402595317269732 98-99 22.646865099468698 26.41559025808464 27.12343304311398 23.814111599332684 100-101 22.651148404207238 26.252193372521514 27.710658528767695 23.385999694503553 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 336.0 1 242.5 2 229.5 3 322.0 4 263.5 5 248.0 6 548.0 7 1371.0 8 2336.0 9 2869.5 10 2718.0 11 2487.0 12 2699.0 13 3017.0 14 3336.0 15 3493.5 16 3573.0 17 3683.0 18 3853.0 19 4052.5 20 4266.5 21 4652.0 22 5292.0 23 6096.5 24 7018.5 25 8424.0 26 10287.5 27 12437.0 28 13987.5 29 16267.0 30 20209.5 31 23896.5 32 28224.5 33 32062.5 34 35682.0 35 39949.5 36 45277.5 37 50757.0 38 54607.5 39 56754.0 40 58915.0 41 63691.5 42 68244.0 43 74056.0 44 79228.5 45 80930.5 46 81040.0 47 84819.5 48 91714.0 49 91759.0 50 88625.5 51 85092.0 52 80103.5 53 79530.0 54 80445.0 55 75552.5 56 68524.5 57 63607.0 58 59590.5 59 53970.5 60 45495.5 61 36456.0 62 28922.0 63 22071.0 64 16666.0 65 13259.5 66 10362.0 67 8259.5 68 6005.0 69 3804.0 70 2652.0 71 1960.5 72 1535.5 73 1339.5 74 1107.5 75 891.5 76 600.5 77 352.5 78 239.5 79 172.5 80 108.5 81 70.5 82 48.0 83 33.0 84 19.5 85 11.5 86 5.5 87 3.0 88 1.0 89 2.0 90 1.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.016864359418968997 2 0.0 3 0.0 4 4.485201973130052E-5 5 0.04041166977790177 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.004530053992861353 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 6.952063058351581E-4 24-25 1.121300493282513E-4 26-27 9.194664044916607E-4 28-29 9.418924143573109E-4 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 4.7094620717865544E-4 42-43 0.010921466804571676 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 2.242600986565026E-5 54-55 1.121300493282513E-4 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 4.485201973130052E-5 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 6.952063058351581E-4 84-85 4.485201973130052E-5 86-87 0.0 88-89 0.0 90-91 0.012289453406376343 92-93 0.0021304709372367745 94-95 0.0 96-97 0.0 98-99 0.0015025426609985675 100-101 0.017738973803729356 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2229554.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 40.32320863230742 #Duplication Level Percentage of deduplicated Percentage of total 1 66.98190630775106 27.009253826371143 2 15.478308616142419 12.482701352079046 3 6.481125566484912 7.840193351686582 4 3.2581172517637853 5.255109667655648 5 1.972159099710347 3.976189141686195 6 1.200956836577121 2.9055859847817107 7 0.8477794410842678 2.3929631093915336 8 0.6098267637848807 1.9672137460530075 9 0.4364714133105344 1.5839935074862883 >10 2.4169030703198446 17.833105727191978 >50 0.2007835372645178 5.620110407476946 >100 0.10896606825188516 7.9702428957395295 >500 0.00524522158436184 1.3782421359710075 >1k 0.0011160045924174095 0.6049469106950799 >5k 2.232009184834819E-4 0.6872535312158937 >10k+ 1.1160045924174095E-4 0.4928947045185755 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10953 0.4912641721169346 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9168 0.41120331689656314 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6104 0.27377672843985834 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2237 0.10033396813891927 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 4.485201973130052E-5 0.0 16-17 0.0 0.0 0.0 4.485201973130052E-5 0.0 18-19 0.0 0.0 0.0 4.485201973130052E-5 0.0 20-21 0.0 0.0 0.0 4.485201973130052E-5 0.0 22-23 0.0 0.0 0.0 8.970403946260104E-5 0.0 24-25 0.0 0.0 0.0 2.242600986565026E-4 0.0 26-27 0.0 0.0 0.0 3.8124216771605443E-4 0.0 28-29 0.0 0.0 0.0 8.746143847603601E-4 0.0 30-31 0.0 0.0 0.0 0.0019510628583115726 0.0 32-33 0.0 0.0 0.0 0.003610587588369692 0.0 34-35 0.0 0.0 0.0 0.005292538328293461 0.0 36-37 0.0 0.0 0.0 0.008163067591096695 0.0 38-39 0.0 0.0 0.0 0.013164067791136703 0.0 40-41 0.0 0.0 0.0 0.018456606119430164 0.0 42-43 0.0 0.0 0.0 0.023031512132022817 0.0 44-45 0.0 0.0 0.0 0.02695606385851161 0.0 46-47 0.0 0.0 0.0 0.03240558425586462 0.0 48-49 0.0 0.0 0.0 0.03742901046577028 0.0 50-51 0.0 0.0 0.0 0.04180208238957209 0.0 52-53 0.0 0.0 0.0 0.054001831756485826 0.0 54-55 0.0 0.0 0.0 0.07382642447772066 0.0 56-57 0.0 0.0 0.0 0.10340633149051334 0.0 58-59 0.0 0.0 0.0 0.1377405525948239 0.0 60-61 0.0 0.0 0.0 0.17689636582024926 0.0 62-63 0.0 0.0 0.0 0.2056913624877442 0.0 64-65 0.0 0.0 0.0 0.24318765098311143 0.0 66-67 0.0 0.0 0.0 0.3042761018571427 0.0 68-69 0.0 0.0 0.0 0.38227376416987435 0.0 70-71 0.0 0.0 0.0 0.4649360365346612 0.0 72-73 0.0 0.0 0.0 0.5614575829964199 0.0 74-75 0.0 0.0 0.0 0.6686314841443625 0.0 76-77 0.0 0.0 0.0 0.7727778739604423 0.0 78-79 0.0 2.242600986565026E-5 0.0 0.8815215957989804 0.0 80-81 0.0 4.485201973130052E-5 0.0 0.9971949546860044 0.0 82-83 0.0 4.485201973130052E-5 0.0 1.1402280456091218 0.0 84-85 0.0 4.485201973130052E-5 0.0 1.3346615511443096 0.0 86-87 0.0 4.485201973130052E-5 0.0 1.5636311118725987 0.0 88-89 0.0 4.485201973130052E-5 0.0 1.8274731179419739 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 6275 0.0 46.58044 1 GTATCAA 10640 0.0 39.48688 1 ATCAACG 13630 0.0 30.541613 3 TATCAAC 13835 0.0 30.192108 2 TCAACGC 13900 0.0 29.982548 4 CAACGCA 14200 0.0 29.315649 5 AACGCAG 14625 0.0 28.482174 6 ACGCAGA 16490 0.0 25.089174 7 CGCAGAG 16600 0.0 24.865692 8 GTACATG 10805 0.0 24.63233 1 TACATGG 11335 0.0 23.393564 2 GAGTACT 9590 0.0 22.759136 12-13 GCAGAGT 17960 0.0 22.533163 9 ACATGGG 11430 0.0 22.450771 3 GTACTTT 10065 0.0 21.660492 14-15 AGTACTT 10050 0.0 21.126637 12-13 GGACCGA 745 0.0 21.039095 6 AGAGTAC 15980 0.0 20.643549 10-11 CATGGGA 6260 0.0 20.116632 4 GTACTAG 315 3.1832315E-10 19.614399 1 >>END_MODULE