FastQCFastQC Report
Thu 26 May 2016
SRR937618_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937618_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2233002
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT88180.3948944067224302No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT66320.29699928616275306No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42380.18978935083801984No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN30250.1354678589629566No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA57100.045.0554581
GTATCAA99250.040.6304471
ATCAACG125250.031.726323
TATCAAC125800.031.6660232
TCAACGC127000.031.2919584
CAACGCA128900.030.7193855
AACGCAG132800.029.871386
ACGCAGA148550.026.6409057
GTACATG117250.026.4436281
CGCAGAG150450.026.2413068
TACATGG124550.024.6557922
ACATGGG122600.023.921363
GCAGAGT161100.023.7987779
GAGTACT75450.022.79121412-13
GTACACG3251.8189894E-1221.947961
AGTACTT81650.020.85696412-13
GTACTTT82400.020.6959514-15
ACCGATA1150.003720237420.6561228
CATGGGG58550.020.6222954
AGAGTAC144200.020.45734810-11
Produced by FastQC (version 0.11.5)