FastQCFastQC Report
Thu 26 May 2016
SRR937618_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937618_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2233002
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT110630.4954317103164261No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT88820.3977605035732167No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61110.2736674664868191No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA63900.046.1014141
GTATCAA107350.038.285781
ATCAACG138700.029.4555823
TCAACGC138950.029.3683974
TATCAAC141300.028.9808242
CAACGCA141250.028.8222755
AACGCAG145200.028.1319396
ACGCAGA165500.024.5990737
GTACATG113850.024.4144131
CGCAGAG167950.024.1553738
TACATGG118300.023.411512
ACATGGG116850.022.72633
GAGTACT94750.022.2858112-13
GCAGAGT179600.022.244659
AGTACTT98350.021.54251512-13
GTAAGGT6050.021.2008864
GTACTTT100600.020.9629714-15
AGAGTAC160000.020.5457510-11
CATGGGG54550.019.942814
TAAGGTG8100.019.9402145
Produced by FastQC (version 0.11.5)