##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937618_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2233002 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.179678746369234 33.0 31.0 34.0 31.0 34.0 2 32.37634449051098 34.0 31.0 34.0 31.0 34.0 3 32.47334977756402 34.0 31.0 34.0 31.0 34.0 4 36.11626724920085 37.0 35.0 37.0 35.0 37.0 5 36.0260738682724 37.0 35.0 37.0 35.0 37.0 6 36.061518529763966 37.0 35.0 37.0 35.0 37.0 7 36.004966408449256 37.0 35.0 37.0 35.0 37.0 8 35.982644440085586 37.0 35.0 37.0 35.0 37.0 9 37.65386775291737 39.0 37.0 39.0 35.0 39.0 10-11 37.57775250537169 39.0 37.0 39.0 35.0 39.0 12-13 37.474363883238794 39.0 37.0 39.0 35.0 39.0 14-15 38.68634421285785 40.0 38.0 41.0 34.5 41.0 16-17 38.64454845987599 40.0 38.0 41.0 34.0 41.0 18-19 38.62207736491055 40.0 38.0 41.0 34.0 41.0 20-21 38.50558508232415 40.0 38.0 41.0 34.0 41.0 22-23 38.3437578649728 40.0 38.0 41.0 34.0 41.0 24-25 38.41455605503265 40.0 38.0 41.0 34.0 41.0 26-27 38.29576843191363 40.0 38.0 41.0 34.0 41.0 28-29 38.147752890503455 40.0 38.0 41.0 33.5 41.0 30-31 38.197199106852565 40.0 38.0 41.0 34.0 41.0 32-33 38.135715507643965 40.0 37.0 41.0 34.0 41.0 34-35 38.0011448713436 40.0 37.0 41.0 33.0 41.0 36-37 37.8310104514013 40.0 37.0 41.0 33.0 41.0 38-39 37.68428241443581 40.0 37.0 41.0 33.0 41.0 40-41 37.49583878563476 40.0 37.0 41.0 33.0 41.0 42-43 37.26027182241664 40.0 36.5 41.0 32.0 41.0 44-45 36.93374255822431 39.5 36.0 41.0 31.0 41.0 46-47 36.56823280946457 39.0 36.0 41.0 30.5 41.0 48-49 36.22875057881721 39.0 35.0 41.0 30.0 41.0 50-51 35.94934554469723 39.0 35.0 40.0 29.0 41.0 52-53 35.68045147294987 39.0 35.0 40.0 28.5 41.0 54-55 35.428101497446036 38.0 35.0 40.0 28.0 41.0 56-57 35.13672782200822 38.0 34.0 40.0 27.5 41.0 58-59 34.83179123887932 38.0 34.0 40.0 27.0 41.0 60-61 34.45012924305487 37.0 34.0 40.0 26.0 41.0 62-63 34.07407606441911 37.0 33.5 39.5 26.0 41.0 64-65 33.64562212662595 36.0 33.0 39.0 25.5 40.5 66-67 33.19353833986714 36.0 32.5 39.0 24.0 40.0 68-69 32.74089342508426 35.0 32.0 38.5 23.0 40.0 70-71 32.31809487855362 35.0 31.5 37.5 22.0 40.0 72-73 31.817894027860255 35.0 31.0 37.0 20.5 39.0 74-75 31.312063088165615 34.5 31.0 36.5 19.5 39.0 76-77 30.10564567340289 33.5 29.5 35.0 19.5 37.0 78-79 30.735223703337482 34.0 30.5 35.5 19.0 37.0 80-81 30.55055705279261 34.0 30.5 35.0 18.0 37.0 82-83 30.202806580558367 34.0 30.0 35.0 16.5 36.5 84-85 29.825097559249834 34.0 30.0 35.0 11.5 36.0 86-87 29.405936716581536 34.0 29.0 35.0 7.0 36.0 88-89 29.02345497227499 34.0 29.0 35.0 3.5 35.0 90-91 28.472150942990645 33.5 29.0 35.0 2.0 35.0 92-93 27.95873044448684 33.0 27.0 35.0 2.0 35.0 94-95 27.452455035866514 33.0 26.5 35.0 2.0 35.0 96-97 26.8504992382452 33.0 25.5 34.5 2.0 35.0 98-99 25.88168125241267 32.0 24.5 34.0 2.0 35.0 100-101 23.140585409238327 29.5 10.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.30376322750476703 1101 2 0.3088584147994311 1101 3 0.22002818886746667 1101 4 0.15484248088142039 1101 5 0.10460682589877024 1101 6 0.14324373211597674 1101 7 0.1351655470799784 1101 8 0.20952366650642062 1101 9 0.3011761657619161 1101 10-11 0.20979622890137506 1101 12-13 0.2609359469493313 1101 14-15 0.2957866138091063 1101 16-17 0.26519117131285697 1101 18-19 0.33766060739955606 1101 20-21 0.24947279235504283 1101 22-23 0.29116408475726985 1101 24-25 0.15604068548787353 1101 26-27 0.20397743775976096 1101 28-29 0.23763416292910478 1101 30-31 0.343054259650728 1101 32-33 0.2711741489893882 1101 34-35 0.3127996532068451 1101 36-37 0.29149128073939323 1101 38-39 0.15193310717345554 1101 40-41 0.20583712310155278 1101 42-43 0.27365916775302424 1101 44-45 0.3022437978772885 1101 46-47 0.3841888205942041 1101 48-49 0.3897833820340182 1101 50-51 0.3509570458310094 1101 52-53 0.33425899395872705 1101 54-55 0.3901929656257721 1101 56-57 0.3544199527269072 1101 58-59 0.3219408504019654 1101 60-61 0.33032039555998693 1101 62-63 0.39783162565007757 1101 64-65 0.4429774266723072 1101 66-67 0.2830189410352375 1101 68-69 0.3657070964402678 1101 70-71 0.3932563972779377 1101 72-73 0.331939457103811 1101 74-75 0.3323903541634259 1101 76-77 0.3293589933831491 1101 78-79 0.3859261856896872 1101 80-81 0.32439506324046974 1101 82-83 0.40730447916517676 1101 84-85 0.3475434214011379 1101 86-87 0.14701771819649068 1101 88-89 0.6478692800414585 1101 90-91 0.5284237753985153 1101 92-93 0.4804561741468554 1101 94-95 0.45991629775766185 1101 96-97 0.7833965256092235 1101 98-99 0.9001213702742703 1101 100-101 1.4746053839555024 1102 1 0.2728441610957617 1102 2 0.23440868564554762 1102 3 0.22471410348093457 1102 4 0.12138904998662525 1102 5 0.12956224686924145 1102 6 0.17357929062914224 1102 7 0.21611735015724776 1102 8 0.21887493952193893 1102 9 0.2587448996922319 1102 10-11 0.2705263097972761 1102 12-13 0.2538450457596113 1102 14-15 0.3122665645607512 1102 16-17 0.38952568650812935 1102 18-19 0.3412418961261565 1102 20-21 0.32204631642357384 1102 22-23 0.2832886214986985 1102 24-25 0.2112956014427212 1102 26-27 0.2457839031931428 1102 28-29 0.2705641317034946 1102 30-31 0.3309967794347273 1102 32-33 0.25623717631707166 1102 34-35 0.29410846718737815 1102 36-37 0.3437136145581974 1102 38-39 0.32404981452278747 1102 40-41 0.33989436565889974 1102 42-43 0.3695989733958456 1102 44-45 0.4080379627622719 1102 46-47 0.5061144723838851 1102 48-49 0.40613828249957606 1102 50-51 0.4391331851545175 1102 52-53 0.5885991044104983 1102 54-55 0.5554221700877662 1102 56-57 0.3795835504173297 1102 58-59 0.43839314042701716 1102 60-61 0.3648054236958487 1102 62-63 0.2590881078439722 1102 64-65 0.30675483968752815 1102 66-67 0.33235285667434766 1102 68-69 0.35139474408661187 1102 70-71 0.24458269666039456 1102 72-73 0.4331445379501311 1102 74-75 0.37037715094490054 1102 76-77 0.17655827268959 1102 78-79 0.3530077572445087 1102 80-81 0.431774851327571 1102 82-83 0.5825200353847002 1102 84-85 0.5199490877749078 1102 86-87 0.4054092054726439 1102 88-89 0.47886936316789175 1102 90-91 0.5581381396282552 1102 92-93 0.6288348750988781 1102 94-95 0.63236379782483 1102 96-97 0.6891435915255997 1102 98-99 0.6691302309029759 1102 100-101 0.9838947178603235 1103 1 0.243838888246529 1103 2 0.1566739985559522 1103 3 0.1311827057815549 1103 4 0.06263874972640338 1103 5 0.020413166743843192 1103 6 0.07622603824470531 1103 7 0.14682748746572827 1103 8 0.13608090152140306 1103 9 0.13896511879601547 1103 10-11 0.10391820628144899 1103 12-13 0.18410968803621586 1103 14-15 0.25232614196400505 1103 16-17 0.2603961045732319 1103 18-19 0.13244314816841296 1103 20-21 -0.001064002295315447 1103 22-23 0.20826326970019693 1103 24-25 0.22049272320372637 1103 26-27 0.10577132068812745 1103 28-29 0.08583492897627565 1103 30-31 0.19544483211521424 1103 32-33 0.17180571622852625 1103 34-35 0.20222297375809006 1103 36-37 0.15892765339973636 1103 38-39 0.09508702477280195 1103 40-41 0.011508129677743284 1103 42-43 0.1320386799701012 1103 44-45 0.1321315288105822 1103 46-47 0.06790229619344501 1103 48-49 0.04646723234949235 1103 50-51 0.1344517605738389 1103 52-53 0.007757592539142877 1103 54-55 0.11701264780968756 1103 56-57 -0.03050631198544096 1103 58-59 -0.05513468392755527 1103 60-61 -0.16518035330902592 1103 62-63 -0.08445172691950376 1103 64-65 0.0666932951399204 1103 66-67 -0.08779488849996397 1103 68-69 -0.05869024557325275 1103 70-71 0.04276713070124316 1103 72-73 0.04599708315519635 1103 74-75 0.09329999539233214 1103 76-77 -0.014089056767563335 1103 78-79 -0.029455457607181046 1103 80-81 -0.08478597755848227 1103 82-83 -0.05173585255809243 1103 84-85 -0.04106306755297595 1103 86-87 0.04758077402843952 1103 88-89 0.0042342458872894895 1103 90-91 0.04790781589946036 1103 92-93 0.11391237066062132 1103 94-95 0.3992917517943759 1103 96-97 0.3874956298751684 1103 98-99 0.3586467230622823 1103 100-101 0.609272558466408 1104 1 0.22702729092993934 1104 2 0.26535855820489473 1104 3 0.11477573263893248 1104 4 0.11294045732397251 1104 5 0.0448696544269751 1104 6 0.10544133590307325 1104 7 0.16671912218916418 1104 8 0.13897056265761165 1104 9 0.23367343430022203 1104 10-11 0.2348672409424637 1104 12-13 0.23627815730954893 1104 14-15 0.25287190716620245 1104 16-17 0.2424711939874129 1104 18-19 0.21387585194711534 1104 20-21 0.2639209675279517 1104 22-23 0.3471913975853056 1104 24-25 0.1087114160050433 1104 26-27 0.1611027812918735 1104 28-29 0.1635048666534118 1104 30-31 0.28329423075894766 1104 32-33 0.26394096812498447 1104 34-35 0.22667050957897317 1104 36-37 0.22997918091567016 1104 38-39 0.182228927902905 1104 40-41 0.19616505552468766 1104 42-43 0.3799327543744013 1104 44-45 0.4561614900967683 1104 46-47 0.31971131835766187 1104 48-49 0.20614547854837895 1104 50-51 0.21708998180299943 1104 52-53 0.04315027194490284 1104 54-55 0.01143617473599079 1104 56-57 0.1643539464607997 1104 58-59 0.1726153132376851 1104 60-61 0.09223880093096426 1104 62-63 0.37968084657975254 1104 64-65 0.20385820455602754 1104 66-67 0.2304199430647813 1104 68-69 0.11616767345297774 1104 70-71 0.30409735356083445 1104 72-73 0.27068942256923023 1104 74-75 0.21203152844648443 1104 76-77 0.2845050831046869 1104 78-79 0.3955920917011717 1104 80-81 0.34504932607973515 1104 82-83 0.49979076819148816 1104 84-85 0.3864572845058625 1104 86-87 0.38081175773664455 1104 88-89 0.47820073847007194 1104 90-91 0.5124847001620232 1104 92-93 0.30746237486799544 1104 94-95 0.5076248016520672 1104 96-97 0.8079353572849968 1104 98-99 0.9340276695605816 1104 100-101 1.2437432557388952 1105 1 0.2002924662300245 1105 2 0.17966744434944104 1105 3 0.14684154773978264 1105 4 0.03978730859896018 1105 5 0.060766368791973946 1105 6 0.018029887073822692 1105 7 -0.019711940506809356 1105 8 0.003899691000746941 1105 9 0.12598865701192352 1105 10-11 0.14339047672871175 1105 12-13 0.13276212030596213 1105 14-15 0.12597057861704286 1105 16-17 0.19544153664127606 1105 18-19 0.2220003386626459 1105 20-21 0.20803158506677022 1105 22-23 0.13115316518853604 1105 24-25 0.24214592141898805 1105 26-27 0.11017472201405099 1105 28-29 0.12592988360075452 1105 30-31 0.1009796838931365 1105 32-33 0.10027055153768316 1105 34-35 0.020181160295670963 1105 36-37 -0.01960865549685309 1105 38-39 -0.09387971534672346 1105 40-41 0.038321249652199185 1105 42-43 0.05529018468546809 1105 44-45 0.060564318818983054 1105 46-47 -0.055565420051074454 1105 48-49 0.08688735252565039 1105 50-51 0.1020671566443383 1105 52-53 0.05721605837350552 1105 54-55 0.03580451483801994 1105 56-57 0.04910583934127288 1105 58-59 0.15362097191093227 1105 60-61 0.014208297753462773 1105 62-63 0.024734781129204464 1105 64-65 0.13086275320362262 1105 66-67 -0.04963113449980483 1105 68-69 -0.18463801675974878 1105 70-71 -0.0014208256693990506 1105 72-73 0.009812911900080934 1105 74-75 -0.008097619557620561 1105 76-77 -0.20781075401236038 1105 78-79 -0.037735711984034026 1105 80-81 -0.10947751012184881 1105 82-83 -0.0038721116902173947 1105 84-85 0.043256601287115615 1105 86-87 0.18331115869143133 1105 88-89 0.015509479205352505 1105 90-91 -0.05695948828019226 1105 92-93 0.13600949054209366 1105 94-95 0.1392724115128665 1105 96-97 0.5471663675517107 1105 98-99 0.762047420644496 1105 100-101 0.7597956194586679 1106 1 0.0654156022339194 1106 2 0.1417695490468205 1106 3 0.1059287111553644 1106 4 0.08695189669723646 1106 5 0.07421091962233106 1106 6 0.1547613025800345 1106 7 0.13880255842693145 1106 8 0.012939208185606788 1106 9 0.09181603740927358 1106 10-11 -0.03662850595414113 1106 12-13 -0.20583554663337367 1106 14-15 0.08438152186079861 1106 16-17 0.18366289817370074 1106 18-19 0.2678445790786341 1106 20-21 0.24436060408508098 1106 22-23 0.27873240563357626 1106 24-25 0.28846548700059316 1106 26-27 0.2078252359641226 1106 28-29 0.19931885095241597 1106 30-31 0.2340663080509202 1106 32-33 0.24560393743222164 1106 34-35 0.223508133217301 1106 36-37 0.12704078221192106 1106 38-39 0.14743738769168857 1106 40-41 0.20531136991376542 1106 42-43 0.019718501935834354 1106 44-45 0.09495059468295608 1106 46-47 0.12064565464623911 1106 48-49 0.22855560487302995 1106 50-51 0.12326309598768859 1106 52-53 0.13070256719987583 1106 54-55 0.19767724545095433 1106 56-57 0.1592166449403507 1106 58-59 0.07189941036795489 1106 60-61 0.0690734820424197 1106 62-63 0.049439686580939224 1106 64-65 -0.013439245310010506 1106 66-67 -0.09306564563379993 1106 68-69 -0.10287021146061903 1106 70-71 0.12981983871438985 1106 72-73 0.13022912592123603 1106 74-75 -0.020778390317214956 1106 76-77 -0.09720617628481776 1106 78-79 0.061389800898389524 1106 80-81 0.06524454089739606 1106 82-83 -0.05538437096051396 1106 84-85 0.06109721497966447 1106 86-87 0.24680509936953854 1106 88-89 -0.0703496350220334 1106 90-91 0.008999269870269444 1106 92-93 0.3081863660864954 1106 94-95 0.5014277344345324 1106 96-97 0.26233351093338086 1106 98-99 0.3713777680968313 1106 100-101 0.13498460606592033 1107 1 0.3500517846813409 1107 2 0.4102236580920646 1107 3 0.3153385117511931 1107 4 0.12486940467493213 1107 5 0.17864068783701015 1107 6 0.19765612278646216 1107 7 0.2167502260945824 1107 8 0.2403473506218532 1107 9 0.2405035355320848 1107 10-11 0.30504941439866684 1107 12-13 0.40975208846045064 1107 14-15 0.45366190553829 1107 16-17 0.470312276760545 1107 18-19 0.5195526081185236 1107 20-21 0.49513117226258174 1107 22-23 0.5501737237287685 1107 24-25 0.685217710016822 1107 26-27 0.5667714489169242 1107 28-29 0.6627887623831228 1107 30-31 0.5897636389580256 1107 32-33 0.5525072592483511 1107 34-35 0.5839437712942228 1107 36-37 0.5019141959522031 1107 38-39 0.5581940037540747 1107 40-41 0.6157094075504759 1107 42-43 0.6798116558222205 1107 44-45 0.6884741101172764 1107 46-47 0.594520574104223 1107 48-49 0.7553080205667229 1107 50-51 0.6258021882614884 1107 52-53 0.5959325022505695 1107 54-55 0.6035897234134779 1107 56-57 0.49888321092360144 1107 58-59 0.5178530986396126 1107 60-61 0.5627917717239157 1107 62-63 0.661351715718304 1107 64-65 0.6312595459789279 1107 66-67 0.6769480174651221 1107 68-69 0.7366796146479686 1107 70-71 0.7772311319938581 1107 72-73 0.703956873799374 1107 74-75 0.6748971907045132 1107 76-77 0.6088885802247042 1107 78-79 0.7799581563452413 1107 80-81 0.8193457373677724 1107 82-83 0.8585007037331742 1107 84-85 0.9276395952377037 1107 86-87 1.126683026862743 1107 88-89 1.0369983467098791 1107 90-91 1.1378317804519469 1107 92-93 1.088482205330024 1107 94-95 1.3746159287463513 1107 96-97 1.6575888000714158 1107 98-99 1.7611610172848948 1107 100-101 1.9900514849800324 1108 1 0.1565257205880073 1108 2 0.19694729904456665 1108 3 0.11752130942221584 1108 4 0.08991480434809063 1108 5 0.03776537885370601 1108 6 -0.023139582790427937 1108 7 0.10893633601187958 1108 8 0.029888317086047778 1108 9 0.10737806162081398 1108 10-11 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2316 76-77 0.057162120360896296 2316 78-79 0.08092360162017798 2316 80-81 -0.21818050024799618 2316 82-83 -0.19647416702589382 2316 84-85 -0.13684685080983527 2316 86-87 -0.030988958787517618 2316 88-89 0.1133136659348537 2316 90-91 0.36680417762054773 2316 92-93 0.4619024130220133 2316 94-95 0.3382601922618811 2316 96-97 0.5384674196120507 2316 98-99 0.6165688966186913 2316 100-101 1.0857556794038281 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 7.0 4 17.0 5 39.0 6 92.0 7 240.0 8 540.0 9 1025.0 10 1804.0 11 2996.0 12 4455.0 13 6512.0 14 8563.0 15 11700.0 16 16367.0 17 22769.0 18 20654.0 19 12827.0 20 11219.0 21 11542.0 22 11985.0 23 13502.0 24 15442.0 25 18542.0 26 22963.0 27 28409.0 28 35363.0 29 44682.0 30 56482.0 31 71516.0 32 91300.0 33 119938.0 34 165911.0 35 240139.0 36 358460.0 37 483752.0 38 303277.0 39 17967.0 40 3.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.90695736927162 19.2321577663032 15.298750138853553 22.562134725571628 2 15.75063524349732 20.085830644128396 39.66279474895231 24.500739363421975 3 19.431733603462963 24.282378609602677 29.789986753258617 26.495901033675747 4 13.4050009068538 16.579795297347285 35.87043410307681 34.14476969272211 5 14.082390393774785 37.44132617295022 33.265904252197906 15.210379181077094 6 33.059218039213576 35.59875898006361 16.547096688672916 14.794926292049896 7 29.602391757821984 32.893029204631254 19.478845070447765 18.025733967099 8 27.465985252140392 34.66826272435045 18.714582432080224 19.15116959142894 9 26.976867911448355 16.447589388634672 19.86012551712896 36.715417182788016 10-11 25.840136211313837 26.629656681748017 25.912729103121762 21.61747800381639 12-13 26.40482221453919 23.72691135716704 27.24005291735112 22.62821351094264 14-15 23.10481584879906 24.92987019268232 25.683855186873995 26.28145877164463 16-17 22.69841227191019 28.5042736191011 26.223890529430783 22.57342357955792 18-19 22.62848398702733 26.401387011744724 29.099772413996945 21.870356587231 20-21 23.219638853883694 25.458553104744198 29.357474825369618 21.964333216002494 22-23 23.24399015397127 25.272757521725996 29.368223605972553 22.11502871833018 24-25 22.875040612377557 25.536727136099103 29.381529748188704 22.20670250333464 26-27 22.507397584183554 25.995161201840133 29.207778351248383 22.289662862727933 28-29 22.42026581495059 25.939147740555008 29.448132586827843 22.192453857666564 30-31 22.417198909808413 25.76388198487955 29.298988536508254 22.51993056880379 32-33 22.28562715125199 25.813187807265734 29.285038705742316 22.61614633573996 34-35 22.939388321192727 25.695968924344896 29.115379207004743 22.249263547457637 36-37 22.893284466382028 25.463143337981787 28.916051127585195 22.72752106805099 38-39 22.578521590120555 25.827513249549206 29.093052151130284 22.50091300919995 40-41 22.76366403140903 25.71938906089306 29.12157854821017 22.395368359487744 42-43 22.716028536262446 25.74247492237986 28.767958335050263 22.773538206307432 44-45 22.65618212612438 25.933048873220894 28.669499624272614 22.74126937638211 46-47 22.890955762690762 26.260836309147955 28.10167657709218 22.7465313510691 48-49 23.074094873179693 26.393348505733538 27.740772287709547 22.791784333377223 50-51 23.039567362680373 26.52572635402924 27.655550689161945 22.77915559412844 52-53 23.36130912556281 26.498028214932184 27.43114426229802 22.70951839720699 54-55 23.057010248983207 26.546550338960735 27.500154500533363 22.896284911522695 56-57 22.991985676680986 26.828972835671443 27.46289524147314 22.716146246174432 58-59 23.304143928218604 26.273151569053677 27.463947636410534 22.95875686631718 60-61 23.118004372589006 26.401028749638378 27.841623070646598 22.63934380712601 62-63 22.94988987918506 26.581637634001225 27.83080803331121 22.637664453502502 64-65 22.869012208676928 26.39847613213065 27.734435526703514 22.998076132488908 66-67 22.884081608525207 26.55505906398651 27.60400572861108 22.956853598877206 68-69 22.9301938782776 26.32679190631075 27.83044237816497 22.91257183724668 70-71 22.948181158042733 26.48082703071495 27.651449249563537 22.91954256167878 72-73 23.108040207756193 26.466635497863418 27.31370146556071 23.111622828819677 74-75 22.884283130960025 26.74030296435023 27.29437770319955 23.08103620149019 76-77 23.090798843888184 26.387638703413614 27.526755461929724 22.99480699076848 78-79 22.949598791223654 26.581010675315113 27.43945146488897 23.029939068572265 80-81 22.845068776294497 26.977535383444835 27.310070015658304 22.867325824602368 82-83 22.95631226877675 26.858552388510144 27.22141139052585 22.963723952187255 84-85 22.882086311491726 26.715639790263605 27.20538595387761 23.196887944367056 86-87 22.995389166691297 26.647468296042724 27.24883363293002 23.108308904335956 88-89 22.917422375797244 26.740773183364812 27.14077282510271 23.20103161573523 90-91 22.805927714771723 26.620165301693056 27.40101291402682 23.172894069508406 92-93 22.987562199080948 26.347712063542815 27.42590410627013 23.238821631106102 94-95 22.84335168530973 26.309403215939795 27.586249362965194 23.26099573578528 96-97 22.980543680659487 26.245789300681327 27.417328779821958 23.356338238837225 98-99 22.648969698326773 26.368042454810592 27.17369861786752 23.809289228995112 100-101 22.61963629848607 26.251522888112515 27.769954313356628 23.35888650004479 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 312.0 1 232.5 2 231.5 3 328.0 4 277.5 5 264.5 6 562.5 7 1384.5 8 2371.5 9 2865.5 10 2720.0 11 2542.0 12 2715.0 13 3038.0 14 3340.5 15 3516.5 16 3645.0 17 3754.5 18 3869.0 19 4005.5 20 4257.0 21 4672.0 22 5270.5 23 5987.0 24 6960.5 25 8518.0 26 10428.0 27 12557.0 28 14128.0 29 16407.0 30 20197.5 31 23867.0 32 28381.0 33 32218.0 34 35745.5 35 40061.0 36 45309.5 37 50883.5 38 54599.5 39 56441.5 40 58985.0 41 63788.5 42 68146.0 43 74040.5 44 79207.5 45 80915.5 46 81540.5 47 85078.5 48 91567.0 49 91945.0 50 88541.0 51 84751.0 52 80307.5 53 79945.5 54 80320.0 55 75640.5 56 68983.5 57 63817.0 58 59715.0 59 54039.0 60 45292.0 61 36318.5 62 28991.5 63 22181.5 64 16845.5 65 13315.0 66 10283.0 67 8369.5 68 6153.5 69 3974.5 70 2778.0 71 2017.5 72 1593.0 73 1329.0 74 1089.5 75 855.5 76 596.5 77 379.5 78 237.5 79 150.0 80 95.0 81 67.5 82 41.5 83 23.0 84 16.5 85 7.5 86 4.5 87 5.0 88 3.0 89 2.0 90 1.0 91 0.5 92 0.5 93 0.0 94 0.0 95 0.5 96 0.5 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.019435719269396086 2 0.0 3 0.0 4 3.134793430547756E-4 5 0.023869212835456483 6 0.0 7 0.0 8 0.0 9 0.0 10-11 4.478276329353937E-5 12-13 0.003851317643244386 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 9.180466475175571E-4 24-25 2.2391381646769685E-5 26-27 4.2543625128862403E-4 28-29 8.956552658707874E-4 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 6.717414494030905E-5 40-41 6.045673044627816E-4 42-43 0.01495744294004215 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 1.343482898806181E-4 70-71 6.717414494030905E-5 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 6.717414494030905E-5 82-83 0.0018360932950351142 84-85 3.8065348799508467E-4 86-87 0.0 88-89 0.0 90-91 0.010568732137275293 92-93 0.0022839209279705078 94-95 0.0 96-97 0.0 98-99 0.001768919150094805 100-101 0.018002670844002824 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2233002.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 40.45589655242169 #Duplication Level Percentage of deduplicated Percentage of total 1 66.9790145042893 27.0969608196868 2 15.694134660726194 12.698405766282292 3 6.321007272389262 7.671660489566556 4 3.257790783060291 5.2718738763568 5 1.9540579699847471 3.952658354556903 6 1.2142530997836505 2.947421867598284 7 0.8337669339447724 2.3611552179949666 8 0.6006438119766077 1.9439667137742294 9 0.42755939377927266 1.5567568744275346 >10 2.4018265326829056 17.785716526227898 >50 0.19954922096960906 5.57019739329882 >100 0.109845570681259 8.014745452218973 >500 0.004995950134694539 1.3036458898051726 >1k 0.0012212322551475483 0.6545402774205239 >5k 2.2204222820864514E-4 0.6734044039913482 >10k+ 1.1102111410432257E-4 0.4968900767929223 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11063 0.4954317103164261 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8882 0.3977605035732167 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6111 0.2736674664868191 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 4.478276329353937E-5 0.0 14-15 0.0 0.0 0.0 4.478276329353937E-5 0.0 16-17 0.0 0.0 0.0 4.478276329353937E-5 0.0 18-19 0.0 0.0 0.0 6.717414494030905E-5 0.0 20-21 0.0 0.0 0.0 8.956552658707874E-5 0.0 22-23 0.0 0.0 0.0 8.956552658707874E-5 0.0 24-25 0.0 0.0 0.0 1.1195690823384842E-4 0.0 26-27 0.0 0.0 0.0 1.7913105317415748E-4 0.0 28-29 0.0 0.0 0.0 5.597845411692421E-4 0.0 30-31 0.0 0.0 0.0 0.0015226139519803387 0.0 32-33 0.0 0.0 0.0 0.0028213140874929804 0.0 34-35 0.0 0.0 0.0 0.004903712580642561 0.0 36-37 0.0 0.0 0.0 0.008374376735891862 0.0 38-39 0.0 0.0 0.0 0.013815482476056896 0.0 40-41 0.0 0.0 0.0 0.018808760583286538 0.0 42-43 0.0 0.0 0.0 0.023846821453809716 0.0 44-45 0.0 0.0 0.0 0.02760857357046702 0.0 46-47 0.0 0.0 0.0 0.033430332798627144 0.0 48-49 0.0 0.0 0.0 0.03826687123432939 0.0 50-51 0.0 0.0 0.0 0.04393189079096212 0.0 52-53 0.0 0.0 0.0 0.05785933017525287 0.0 54-55 0.0 0.0 0.0 0.08060897392837087 0.0 56-57 0.0 0.0 0.0 0.11220321343196289 0.0 58-59 0.0 0.0 0.0 0.14959682078206826 0.0 60-61 0.0 0.0 0.0 0.19395414782431902 0.0 62-63 0.0 0.0 0.0 0.22407055613922422 0.0 64-65 0.0 0.0 0.0 0.2631659084944841 0.0 66-67 0.0 0.0 0.0 0.33233288640135566 0.0 68-69 0.0 0.0 0.0 0.4190994902825882 0.0 70-71 0.0 0.0 0.0 0.5096502376621248 0.0 72-73 0.0 0.0 0.0 0.6140836416626586 0.0 74-75 0.0 0.0 0.0 0.7234879323887753 0.0 76-77 0.0 0.0 0.0 0.8307426504768021 0.0 78-79 0.0 0.0 0.0 0.9410873792320831 0.0 80-81 0.0 0.0 0.0 1.0579256086649274 0.0 82-83 0.0 0.0 0.0 1.2021037150884772 0.0 84-85 0.0 0.0 0.0 1.4048576758999767 0.0 86-87 0.0 0.0 0.0 1.6420047989209146 0.0 88-89 0.0 0.0 0.0 1.9060215799179758 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 6390 0.0 46.101414 1 GTATCAA 10735 0.0 38.28578 1 ATCAACG 13870 0.0 29.455582 3 TCAACGC 13895 0.0 29.368397 4 TATCAAC 14130 0.0 28.980824 2 CAACGCA 14125 0.0 28.822275 5 AACGCAG 14520 0.0 28.131939 6 ACGCAGA 16550 0.0 24.599073 7 GTACATG 11385 0.0 24.414413 1 CGCAGAG 16795 0.0 24.155373 8 TACATGG 11830 0.0 23.41151 2 ACATGGG 11685 0.0 22.7263 3 GAGTACT 9475 0.0 22.28581 12-13 GCAGAGT 17960 0.0 22.24465 9 AGTACTT 9835 0.0 21.542515 12-13 GTAAGGT 605 0.0 21.200886 4 GTACTTT 10060 0.0 20.96297 14-15 AGAGTAC 16000 0.0 20.54575 10-11 CATGGGG 5455 0.0 19.94281 4 TAAGGTG 810 0.0 19.940214 5 >>END_MODULE