FastQCFastQC Report
Thu 26 May 2016
SRR937617_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937617_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2157427
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT86750.4020993526084544No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT66720.30925727730300956No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43460.2014436641425179No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN31380.14545103959485073No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA55900.044.9936331
GTATCAA97200.039.9145361
ATCAACG121750.031.6141093
TCAACGC123100.031.268864
TATCAAC124400.031.019222
CAACGCA124650.030.880045
AACGCAG129400.029.885066
ACGCAGA147350.026.051057
CGCAGAG150100.025.5737678
GTACATG115350.025.2255731
GAGTACT74200.024.96687512-13
TACATGG120600.023.6034092
GCAGAGT160400.023.3375689
GTACTTT79200.023.27073114-15
ACATGGG120750.022.6279723
AGTACTT79600.021.5127412-13
GGACCGA5750.020.6555216
AGAGTAC143100.020.59731910-11
GTGTAAG5700.020.0190541
CATGGGG56800.019.9956114
Produced by FastQC (version 0.11.5)