FastQCFastQC Report
Thu 26 May 2016
SRR937617_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937617_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2157427
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT108300.5019868574927447No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT89630.4154485875999513No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59650.27648675945930035No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA62400.046.3065221
GTATCAA105650.038.5059131
ATCAACG135650.029.8683193
TCAACGC136300.029.7955764
CAACGCA137550.029.4902735
TATCAAC138500.029.3222892
AACGCAG141550.028.723376
ACGCAGA160950.025.115417
GTACATG104700.025.0108281
CGCAGAG163000.024.7995418
TACATGG110400.023.3190982
GAGTACT94100.023.0942112-13
GCAGAGT174150.022.9935349
ACATGGG109150.022.759333
AGTACTT98950.022.20228212-13
GTACTTT99150.022.01224114-15
ATGGGAG22050.021.9721785
AGAGTAC153600.021.1836610-11
CATGGGA59950.020.1245444
TACTTTT109350.019.95897114-15
Produced by FastQC (version 0.11.5)