##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937617_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2157427 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.24844502270529 33.0 31.0 34.0 31.0 34.0 2 32.44715487476517 34.0 31.0 34.0 31.0 34.0 3 32.51328781924023 34.0 31.0 34.0 31.0 34.0 4 36.124556705742535 37.0 35.0 37.0 35.0 37.0 5 36.04397089681366 37.0 35.0 37.0 35.0 37.0 6 36.09421547055822 37.0 35.0 37.0 35.0 37.0 7 36.046593465271364 37.0 35.0 37.0 35.0 37.0 8 36.00835995841342 37.0 35.0 37.0 35.0 37.0 9 37.70537311343559 39.0 37.0 39.0 35.0 39.0 10-11 37.622479462804534 39.0 37.0 39.0 35.0 39.0 12-13 37.5180726856575 39.0 37.0 39.0 35.0 39.0 14-15 38.74446968541693 40.0 38.0 41.0 35.0 41.0 16-17 38.7003752618281 40.0 38.0 41.0 34.5 41.0 18-19 38.671727247318216 40.0 38.0 41.0 34.5 41.0 20-21 38.560260208109014 40.0 38.0 41.0 34.0 41.0 22-23 38.50367706531901 40.0 38.0 41.0 34.0 41.0 24-25 38.51583900637194 40.0 38.0 41.0 34.0 41.0 26-27 38.341891753463734 40.0 38.0 41.0 34.0 41.0 28-29 38.30596701533818 40.0 38.0 41.0 34.0 41.0 30-31 38.32586293765675 40.0 38.0 41.0 34.0 41.0 32-33 38.20619005880616 40.0 38.0 41.0 34.0 41.0 34-35 38.056566224488705 40.0 37.0 41.0 33.5 41.0 36-37 37.87655804808227 40.0 37.0 41.0 33.0 41.0 38-39 37.72786448857829 40.0 37.0 41.0 33.0 41.0 40-41 37.54285544771619 40.0 37.0 41.0 33.0 41.0 42-43 37.299444894311605 40.0 36.5 41.0 32.5 41.0 44-45 36.97202130130012 40.0 36.0 41.0 31.0 41.0 46-47 36.60412009305529 39.0 36.0 41.0 30.5 41.0 48-49 36.24747975250148 39.0 35.5 41.0 30.0 41.0 50-51 35.97657742301362 39.0 35.0 40.5 29.5 41.0 52-53 35.69206907116671 39.0 35.0 40.0 28.5 41.0 54-55 35.43295671186094 38.0 35.0 40.0 28.0 41.0 56-57 35.135373294206474 38.0 34.5 40.0 27.5 41.0 58-59 34.853681028373146 38.0 34.0 40.0 27.0 41.0 60-61 34.48460318703715 37.5 34.0 40.0 26.0 41.0 62-63 34.05581602529309 37.0 33.5 39.5 26.0 41.0 64-65 33.639961166704595 36.0 33.0 39.0 25.0 40.5 66-67 33.20676273171699 36.0 32.5 39.0 23.5 40.0 68-69 32.72838501603994 35.0 32.0 38.5 22.5 40.0 70-71 32.296854308396064 35.0 31.5 37.5 22.0 40.0 72-73 31.830321257683345 35.0 31.0 37.0 20.5 39.0 74-75 31.361534596535595 34.5 31.0 36.5 19.5 39.0 76-77 30.124756712509857 33.5 29.5 35.0 18.5 37.0 78-79 30.750973034081802 34.0 30.5 35.5 19.0 37.0 80-81 30.602666741447102 34.0 31.0 35.0 18.0 37.0 82-83 30.235111547227323 34.0 30.0 35.0 16.5 36.5 84-85 29.84179534232213 34.0 30.0 35.0 11.0 36.0 86-87 29.507358997546614 34.0 29.5 35.0 7.0 36.0 88-89 29.1150699421116 34.0 29.0 35.0 3.5 35.0 90-91 28.611332156313978 33.5 29.0 35.0 2.0 35.0 92-93 28.14016835795603 33.0 28.0 35.0 2.0 35.0 94-95 27.632831840891953 33.0 27.0 35.0 2.0 35.0 96-97 27.09505188356315 33.0 26.5 35.0 2.0 35.0 98-99 26.22825198720513 32.5 25.0 34.0 2.0 35.0 100-101 23.71087017080995 30.5 11.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.25181913921721844 1101 2 0.26962032169458183 1101 3 0.31069819618551975 1101 4 0.21273932844027144 1101 5 0.18168032370243026 1101 6 0.19235127189569567 1101 7 0.1644305346909931 1101 8 0.18149744527148925 1101 9 0.16376512619286387 1101 10-11 0.25314205595554284 1101 12-13 0.24948591192720215 1101 14-15 0.30778698441157104 1101 16-17 0.3512522366254416 1101 18-19 0.28971018765246015 1101 20-21 0.27953437907552825 1101 22-23 0.3253569704694286 1101 24-25 0.16063187741413287 1101 26-27 0.2146502258795664 1101 28-29 0.19749783282421163 1101 30-31 0.341777406996691 1101 32-33 0.3569449716175299 1101 34-35 0.350579954745605 1101 36-37 0.2297449320671987 1101 38-39 0.32184358728128615 1101 40-41 0.2852951097997618 1101 42-43 0.2533444600307604 1101 44-45 0.17949951543451448 1101 46-47 0.29280706781914745 1101 48-49 0.25854616240889783 1101 50-51 0.2593633658168599 1101 52-53 0.34545660268761935 1101 54-55 0.2988977816707248 1101 56-57 0.27457907348921395 1101 58-59 0.28477163536365424 1101 60-61 0.3140514043657774 1101 62-63 0.30897396667784705 1101 64-65 0.41976986713134323 1101 66-67 0.45118409217117517 1101 68-69 0.2738966484057812 1101 70-71 0.2201707689760113 1101 72-73 0.20197045334891683 1101 74-75 0.2817270579822342 1101 76-77 0.2438272977761855 1101 78-79 0.3242447988600823 1101 80-81 0.35907231711170695 1101 82-83 0.36376934862804333 1101 84-85 0.3828406208042665 1101 86-87 0.44462208655959046 1101 88-89 0.6140001120424472 1101 90-91 0.6560508197655714 1101 92-93 0.7513027054782739 1101 94-95 0.8275051131947109 1101 96-97 0.9673537211909 1101 98-99 0.9488350865615978 1101 100-101 1.1459116224615755 1102 1 0.18726998208323664 1102 2 0.19927287719756492 1102 3 0.1837376165237785 1102 4 0.16326100545141742 1102 5 0.1072702371428278 1102 6 0.17979465560615893 1102 7 0.1533969221032052 1102 8 0.15269304399860317 1102 9 0.2215633764456797 1102 10-11 0.2691068754606789 1102 12-13 0.23471965996731825 1102 14-15 0.3329079173626681 1102 16-17 0.28190491273666396 1102 18-19 0.34453980532435224 1102 20-21 0.38082154728690654 1102 22-23 0.34527208082596417 1102 24-25 0.2676763261881021 1102 26-27 0.2859465788720499 1102 28-29 0.2556889876016868 1102 30-31 0.3171909540849285 1102 32-33 0.37347414489445185 1102 34-35 0.33558417603388335 1102 36-37 0.4502409102463858 1102 38-39 0.32256278347377787 1102 40-41 0.3597623461084112 1102 42-43 0.41713591911209846 1102 44-45 0.48764047863045334 1102 46-47 0.4243946433152601 1102 48-49 0.40533742666153927 1102 50-51 0.37620213115962287 1102 52-53 0.361215924312674 1102 54-55 0.2837710038589307 1102 56-57 0.39373379530167085 1102 58-59 0.5448309189507299 1102 60-61 0.30497591288720827 1102 62-63 0.37922000748054785 1102 64-65 0.4593403162578582 1102 66-67 0.4011082945332376 1102 68-69 0.30640873242333555 1102 70-71 0.2857244071698588 1102 72-73 0.35926678778627874 1102 74-75 0.414985335882168 1102 76-77 0.27876425677866123 1102 78-79 0.3653940098038895 1102 80-81 0.35692511939769744 1102 82-83 0.4522686110622871 1102 84-85 0.4801120913897705 1102 86-87 0.4129436837837446 1102 88-89 0.4677745004195266 1102 90-91 0.586104815145351 1102 92-93 0.722937874840067 1102 94-95 0.8722538305725038 1102 96-97 0.99325229894146 1102 98-99 1.071955221642824 1102 100-101 1.6417133425205783 1103 1 0.18728094838262876 1103 2 0.19790150229386683 1103 3 0.18243517264480147 1103 4 0.12342788119580206 1103 5 0.1305892894822307 1103 6 0.20097841279348927 1103 7 0.1811440094736696 1103 8 0.08729023028117666 1103 9 0.14840386417967721 1103 10-11 0.17741980038029936 1103 12-13 0.24965859922068034 1103 14-15 0.20634086255427775 1103 16-17 0.1058631045035554 1103 18-19 0.2831020921424283 1103 20-21 0.3488947332357881 1103 22-23 0.265757304325021 1103 24-25 0.16320900830601914 1103 26-27 0.30072268816218894 1103 28-29 0.19887425500510858 1103 30-31 0.30391017804692666 1103 32-33 0.245044581631241 1103 34-35 0.22450212344029552 1103 36-37 0.26339259727446773 1103 38-39 0.3406417071471566 1103 40-41 0.31473785405368915 1103 42-43 0.47687532458984805 1103 44-45 0.522864526001726 1103 46-47 0.4716177804836974 1103 48-49 0.3213489874910138 1103 50-51 0.4262809122711104 1103 52-53 0.3690720049494445 1103 54-55 0.430322964339517 1103 56-57 0.2308062124346364 1103 58-59 0.24516494068044636 1103 60-61 0.2980334620681262 1103 62-63 0.1433426428232778 1103 64-65 0.29898423268370067 1103 66-67 0.30030313316610346 1103 68-69 0.43486463438775047 1103 70-71 0.3496245441311032 1103 72-73 0.39215099893923977 1103 74-75 0.343941438177378 1103 76-77 0.24872531074969473 1103 78-79 0.4062649180523863 1103 80-81 0.5019152634361248 1103 82-83 0.4702953469001905 1103 84-85 0.47621866345382813 1103 86-87 0.5392640947693152 1103 88-89 0.7159054317677516 1103 90-91 0.757279642631751 1103 92-93 0.6931702468075436 1103 94-95 0.6855401485082382 1103 96-97 0.7380245073622511 1103 98-99 0.86997390508375 1103 100-101 1.222284310731233 1104 1 0.20738067138406535 1104 2 0.17785876763665698 1104 3 0.1820234923665751 1104 4 0.11745664688767476 1104 5 0.16558475351807544 1104 6 0.11279764136494208 1104 7 0.1039853118971692 1104 8 0.10640750943318977 1104 9 0.11174671684016602 1104 10-11 0.2244522368696451 1104 12-13 0.10882521839705817 1104 14-15 0.1929642239468521 1104 16-17 0.1745512218176941 1104 18-19 0.260550440129542 1104 20-21 0.26037489216065524 1104 22-23 0.22984870658814316 1104 24-25 0.2518863924486041 1104 26-27 0.24999514063468098 1104 28-29 0.17484838713752993 1104 30-31 0.23889351830759864 1104 32-33 0.03885283103512194 1104 34-35 0.12180934716399605 1104 36-37 0.0733316285474217 1104 38-39 0.14697892104202026 1104 40-41 0.36171946310089 1104 42-43 0.24767821554913638 1104 44-45 0.1389508833661779 1104 46-47 0.06538098929272707 1104 48-49 0.03658670259726193 1104 50-51 0.10030593301464563 1104 52-53 0.20736977850945237 1104 54-55 0.1407553958561465 1104 56-57 0.19803098348561576 1104 58-59 0.05335856639112535 1104 60-61 0.1669223147739416 1104 62-63 0.21261945633431623 1104 64-65 0.27963184910769456 1104 66-67 0.3501638637742772 1104 68-69 0.2130011991435623 1104 70-71 0.2702208578433556 1104 72-73 0.1851143246293887 1104 74-75 0.3531822412157588 1104 76-77 0.3162789419523797 1104 78-79 0.3772587309664708 1104 80-81 0.33223708915784655 1104 82-83 0.19775024084576387 1104 84-85 0.1311281653030889 1104 86-87 0.3325937741009639 1104 88-89 0.22547760332511046 1104 90-91 0.33797535809840795 1104 92-93 0.4054183841798178 1104 94-95 0.49197516672360564 1104 96-97 0.7378073355639536 1104 98-99 0.8764251396735183 1104 100-101 1.178687567296059 1105 1 0.21853223794644094 1105 2 0.29143028348254774 1105 3 0.27302046580251016 1105 4 0.1860493346608365 1105 5 0.14155902271602372 1105 6 0.12687562095816673 1105 7 0.15623760836023592 1105 8 0.18068677256198384 1105 9 0.3192992059938078 1105 10-11 0.2829907629670103 1105 12-13 0.24636763235731252 1105 14-15 0.32576185777867295 1105 16-17 0.2861975845326725 1105 18-19 0.2752456004312762 1105 20-21 0.40164492737375923 1105 22-23 0.37608710909912446 1105 24-25 0.26819486061860687 1105 26-27 0.35500324285282403 1105 28-29 0.3923458733467413 1105 30-31 0.2976397325987534 1105 32-33 0.32077972679392275 1105 34-35 0.45504987387828066 1105 36-37 0.42880518266704115 1105 38-39 0.47204286747323465 1105 40-41 0.5818015969587478 1105 42-43 0.6473886038146404 1105 44-45 0.724092401775934 1105 46-47 0.7099248364141886 1105 48-49 0.6636506900903996 1105 50-51 0.551995330626248 1105 52-53 0.6415170976667355 1105 54-55 0.5746249211002166 1105 56-57 0.6540102251820912 1105 58-59 0.6690304996665049 1105 60-61 0.7045923712190572 1105 62-63 0.7353682615325567 1105 64-65 0.7025512365439468 1105 66-67 0.6378394285259219 1105 68-69 0.6989417474904656 1105 70-71 0.703593633989783 1105 72-73 0.661306297123911 1105 74-75 0.801669184526272 1105 76-77 0.6573253649582718 1105 78-79 0.7376395602570405 1105 80-81 0.8693931204530401 1105 82-83 0.7799043792865312 1105 84-85 0.8477077673429676 1105 86-87 1.0513237438954128 1105 88-89 1.0440527646684146 1105 90-91 1.0701260663664804 1105 92-93 1.2646494796718208 1105 94-95 1.3653967865262686 1105 96-97 1.6319271682716767 1105 98-99 1.7891665537259094 1105 100-101 2.2462955200888715 1106 1 -0.1888806212151195 1106 2 -0.15207893731705724 1106 3 -0.1497211400820504 1106 4 -0.1298186599341946 1106 5 -0.14850780191274282 1106 6 -0.12366707638740593 1106 7 -0.1161548275390274 1106 8 -0.10955758100666912 1106 9 -0.4430207671716673 1106 10-11 -0.34211301860757004 1106 12-13 -0.3481919531349149 1106 14-15 -0.3221225741525089 1106 16-17 -0.2297818042681996 1106 18-19 -0.21404081054613755 1106 20-21 -0.12701306089327602 1106 22-23 -0.22259296174592436 1106 24-25 -0.22115355167198203 1106 26-27 -0.21583020942787812 1106 28-29 -0.3249453859252043 1106 30-31 -0.2787046997130602 1106 32-33 -0.4116627502611294 1106 34-35 -0.5016699384703571 1106 36-37 -0.5122165513952055 1106 38-39 -0.3318489118365875 1106 40-41 -0.42416688436889416 1106 42-43 -0.5729868119063966 1106 44-45 -0.3776431294667759 1106 46-47 -0.42355447507089394 1106 48-49 -0.5382180027256496 1106 50-51 -0.6465608268029683 1106 52-53 -0.7293683643486091 1106 54-55 -0.6984339174025749 1106 56-57 -0.786529623374129 1106 58-59 -0.7632057821340723 1106 60-61 -0.9540010405243748 1106 62-63 -0.8854721491688977 1106 64-65 -1.1827970353887594 1106 66-67 -1.2459136105792084 1106 68-69 -1.1164355892304876 1106 70-71 -1.030790658772979 1106 72-73 -1.0689081471302195 1106 74-75 -1.0193749532718748 1106 76-77 -1.2722088787981853 1106 78-79 -1.1537372614218278 1106 80-81 -1.315991103320254 1106 82-83 -1.3671642224158411 1106 84-85 -1.2429955446274192 1106 86-87 -1.1196641317998335 1106 88-89 -1.2230069635606498 1106 90-91 -1.319669647484229 1106 92-93 -1.134404204841708 1106 94-95 -1.1281009558527764 1106 96-97 -1.3361788804396504 1106 98-99 -1.250377497240656 1106 100-101 -0.6672463993409394 1107 1 0.19284240510925343 1107 2 0.2513239591908558 1107 3 0.11579556624592158 1107 4 0.16395516766456097 1107 5 0.13115298153942945 1107 6 0.13440809592538017 1107 7 0.1524254427379077 1107 8 0.11188069599600681 1107 9 0.2616389649296309 1107 10-11 0.17920852219426564 1107 12-13 0.22054899255882532 1107 14-15 0.18143287699302846 1107 16-17 0.23325922451851966 1107 18-19 0.2835798011515607 1107 20-21 0.23138346225312034 1107 22-23 0.16745821366436076 1107 24-25 0.1492156102491009 1107 26-27 0.18334988190188994 1107 28-29 0.2733245980797534 1107 30-31 0.31607929481121744 1107 32-33 0.2727040247309347 1107 34-35 0.23978663928827615 1107 36-37 0.23156503444795362 1107 38-39 0.2687534759625265 1107 40-41 0.34687253186308453 1107 42-43 0.2869410581727365 1107 44-45 0.37795622029082665 1107 46-47 0.26832344061512003 1107 48-49 0.35902339758670365 1107 50-51 0.3728191227031843 1107 52-53 0.4273016231366853 1107 54-55 0.32799341830028794 1107 56-57 0.34343257149847517 1107 58-59 0.4082997088177862 1107 60-61 0.4336212968389006 1107 62-63 0.4735518491837425 1107 64-65 0.48797874554742293 1107 66-67 0.5994937262547566 1107 68-69 0.6718040723650418 1107 70-71 0.5497593500003575 1107 72-73 0.594657541997659 1107 74-75 0.6884571032139455 1107 76-77 0.4330691926327681 1107 78-79 0.676458985908102 1107 80-81 0.6164131258655345 1107 82-83 0.5839952363342249 1107 84-85 0.7353121139924248 1107 86-87 0.9985707878407055 1107 88-89 0.986916338580194 1107 90-91 0.9135286778950906 1107 92-93 0.9563843663216964 1107 94-95 1.0500570484077336 1107 96-97 1.1365647393106677 1107 98-99 1.4292554435400113 1107 100-101 1.9277577263049146 1108 1 0.07509959311245495 1108 2 0.0873447378509411 1108 3 0.019809853771072028 1108 4 0.05225393055866334 1108 5 -0.004700247524084489 1108 6 0.07477826130373444 1108 7 0.11395429829258319 1108 8 0.10722771191886693 1108 9 0.04692246261217292 1108 10-11 0.047294779487693006 1108 12-13 0.06811651128108309 1108 14-15 0.06910398780292581 1108 16-17 0.07222335384421541 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sequence quality scores pass #Quality Count 2 3.0 3 8.0 4 24.0 5 47.0 6 104.0 7 242.0 8 574.0 9 1121.0 10 1831.0 11 3004.0 12 4423.0 13 6532.0 14 8551.0 15 11833.0 16 16330.0 17 22679.0 18 20275.0 19 12444.0 20 10989.0 21 10985.0 22 11440.0 23 12514.0 24 14351.0 25 17361.0 26 21014.0 27 26089.0 28 32139.0 29 40565.0 30 51104.0 31 65359.0 32 83844.0 33 112067.0 34 154820.0 35 225162.0 36 345113.0 37 483560.0 38 310989.0 39 17935.0 40 2.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.0171545579488 19.19148782265648 15.233271570247627 22.558086049147093 2 15.788714983172083 20.010827712826437 39.65571025114639 24.54474705285509 3 19.433658705485747 24.246104271430738 29.78223596905017 26.538001054033344 4 13.43609772196232 16.56361953382432 35.90610481837856 34.0941779258348 5 14.111309360735355 37.4468816941118 33.22550902483899 15.21629992031385 6 33.03240387739655 35.5624083688579 16.560931146221865 14.844256607523684 7 29.629183281751825 32.919213489031144 19.50856274627137 17.94304048294566 8 27.45511203855333 34.66355987943045 18.72336816031319 19.15795992170303 9 26.951688284238585 16.43726531650897 19.852120141260862 36.75892625799158 10-11 25.809471189523446 26.653509017918104 25.91969508122407 21.617324711334383 12-13 26.44263607053137 23.725547956841613 27.273230846107154 22.558585126519862 14-15 23.14493143916341 24.900773004138728 25.715169041640806 26.239126515057055 16-17 22.68528668640932 28.441773464409227 26.262765785354496 22.610174063826957 18-19 22.661230252518394 26.397092462456435 29.131066775376407 21.81061050964876 20-21 23.183943614183967 25.482103176847183 29.43362494681152 21.900328262157334 22-23 23.15364496724634 25.28441162190005 29.416946614953005 22.144996795900607 24-25 22.87215280053508 25.516552819631904 29.47330315231987 22.137991227513144 26-27 22.47993475478232 25.970734673851553 29.318561536701942 22.23076903466419 28-29 22.332352596942837 25.99017100504428 29.508932658290043 22.16854373972284 30-31 22.412809332598506 25.762030418642208 29.354921394791113 22.470238853968176 32-33 22.26022016040404 25.83331904161763 29.341386753758066 22.565074044220268 34-35 22.88376385388706 25.715354447682355 29.195147738486632 22.205733959943952 36-37 22.85740706148684 25.46155803619229 28.983703227140182 22.697331675180692 38-39 22.554505899852 25.8355902656266 29.150140421900716 22.45976341262068 40-41 22.701013500594573 25.739128177661918 29.170026190963434 22.38983213078007 42-43 22.694496722641876 25.733050563596187 28.834979565335413 22.737473148426528 44-45 22.621460656606228 25.93086579522737 28.711910066945485 22.735763481220918 46-47 22.81173360674544 26.309719865376675 28.123431291070332 22.755115236807548 48-49 23.037349583554857 26.48312086573497 27.732757585772312 22.746771964937864 50-51 23.06047898723804 26.576750916716996 27.663948768602598 22.698821327442364 52-53 23.38591294166616 26.4992280155945 27.46380294675092 22.651056095988416 54-55 23.024510215177614 26.55795537925501 27.550688852971618 22.866845552595755 56-57 22.985667649473193 26.855045385081393 27.47782427864303 22.68146268680238 58-59 23.27116977770279 26.310484665298063 27.444914706268158 22.973430850730985 60-61 23.090630644744873 26.431670689205244 27.915150779145716 22.56254788690417 62-63 23.00453271420076 26.550631840613843 27.868289402144313 22.576546043041084 64-65 22.888213598884228 26.42119524785775 27.771136636372862 22.91945451688516 66-67 22.89743754945127 26.61916254872123 27.602741599136376 22.880658302691124 68-69 22.908654614964956 26.390464196471076 27.851927318977655 22.848953869586317 70-71 22.917484577693706 26.510653662904932 27.65212913345388 22.919732625947482 72-73 23.093852074716782 26.4916727194014 27.346997140575326 23.067478065306496 74-75 22.895884773853297 26.749271238377936 27.285789971109104 23.06905401665966 76-77 23.048473946047768 26.421820993247973 27.574490353555415 22.95521470714884 78-79 22.9484937381427 26.642685940242707 27.436061567784215 22.972758753830373 80-81 22.84320628229831 27.00311528501312 27.344540510524805 22.809137922163764 82-83 22.989268683392787 26.8937862932831 27.220750302212267 22.896194721111847 84-85 22.84852412087367 26.77359501554167 27.209345766668243 23.16853509691642 86-87 23.010280301488763 26.67661524584609 27.247642678060487 23.065461774604657 88-89 22.891110568283423 26.761368982588984 27.16096535363653 23.186555095491066 90-91 22.819464107078456 26.6267217745786 27.428354615333983 23.125459503008962 92-93 22.956140720884665 26.35315941534558 27.4869436357206 23.203756228049155 94-95 22.786379330563676 26.336348808094083 27.611664264885903 23.265607596456334 96-97 22.90856191194418 26.264527142749213 27.440928476374864 23.38598246893174 98-99 22.656635841989715 26.399515346248627 27.181538659781268 23.76231015198039 100-101 22.53908144126403 26.345216539137994 27.781223663313188 23.334478356284784 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 283.0 1 212.5 2 203.0 3 302.5 4 271.5 5 253.0 6 549.0 7 1295.0 8 2235.0 9 2766.5 10 2655.0 11 2490.0 12 2746.0 13 3060.0 14 3298.5 15 3468.0 16 3560.0 17 3756.5 18 3862.5 19 3976.0 20 4184.0 21 4559.5 22 5168.0 23 5865.0 24 6793.0 25 8247.0 26 10109.5 27 12331.5 28 13911.0 29 15941.0 30 19684.5 31 23132.0 32 27334.0 33 31087.0 34 34607.0 35 38707.5 36 43663.5 37 49191.0 38 53060.5 39 55058.0 40 57208.5 41 61916.5 42 66231.0 43 71648.0 44 76524.5 45 78115.5 46 78568.5 47 82424.0 48 88930.0 49 88639.5 50 85501.0 51 81998.0 52 77423.5 53 76766.0 54 76977.0 55 72548.0 56 66372.5 57 61668.0 58 57226.0 59 51873.0 60 43984.0 61 34929.5 62 27642.0 63 21235.0 64 16159.0 65 12823.5 66 9949.5 67 8068.0 68 5864.5 69 3776.5 70 2637.5 71 1875.0 72 1470.5 73 1307.0 74 1073.5 75 838.5 76 578.5 77 339.5 78 207.0 79 125.0 80 87.0 81 62.0 82 39.0 83 23.0 84 11.5 85 6.5 86 4.0 87 5.0 88 4.0 89 2.0 90 1.0 91 1.0 92 1.5 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.037034856799326235 2 0.0 3 0.0 4 0.0 5 0.009919223222848328 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.005446302470489152 14-15 0.0 16-17 0.0 18-19 0.0 20-21 2.3175755193570861E-4 22-23 0.0015991271083563895 24-25 0.0 26-27 0.0012746665356463973 28-29 0.0015991271083563895 30-31 0.0 32-33 0.0 34-35 0.0 36-37 2.781090623228503E-4 38-39 0.0 40-41 1.6223028635499603E-4 42-43 0.01995432522166451 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 1.3905453116142515E-4 84-85 4.635151038714172E-5 86-87 0.0 88-89 0.0 90-91 0.009502059629364052 92-93 0.002317575519357086 94-95 0.0 96-97 0.0 98-99 4.866908590649881E-4 100-101 0.012283150252592556 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2157427.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 40.714227508251646 #Duplication Level Percentage of deduplicated Percentage of total 1 67.37102594188596 27.42959277662269 2 15.41607348697055 12.553070464648904 3 6.356222282993104 7.763660403683999 4 3.26152863480529 5.3116247544855995 5 1.829619876486795 3.7245779952451326 6 1.1967314449805744 2.923439779033071 7 0.8285824196410416 2.361456519982211 8 0.5978581279855898 1.947306547237019 9 0.4417185662853896 1.618580718212588 >10 2.3915605241582214 17.86965604090315 >50 0.19719718174989387 5.593295244609018 >100 0.10627178959783888 7.9667588586832485 >500 0.004121428745915488 1.1327485390964864 >1k 0.0011448413183098572 0.6036167221339598 >5k 2.2896826366197143E-4 0.695813932665309 >10k+ 1.1448413183098571E-4 0.5048007027575895 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10830 0.5019868574927447 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8963 0.4154485875999513 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5965 0.27648675945930035 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 4.635151038714172E-5 0.0 22-23 0.0 0.0 0.0 9.270302077428344E-5 0.0 24-25 0.0 0.0 0.0 9.270302077428344E-5 0.0 26-27 0.0 0.0 0.0 2.5493330712927947E-4 0.0 28-29 0.0 0.0 0.0 8.111514317749801E-4 0.0 30-31 0.0 0.0 0.0 0.001969939191453523 0.0 32-33 0.0 0.0 0.0 0.003661769320584196 0.0 34-35 0.0 0.0 0.0 0.005423126715295582 0.0 36-37 0.0 0.0 0.0 0.00859820517681479 0.0 38-39 0.0 0.0 0.0 0.012885719887625397 0.0 40-41 0.0 0.0 0.0 0.01772945272308171 0.0 42-43 0.0 0.0 0.0 0.023059876417603004 0.0 44-45 0.0 0.0 0.0 0.02695340329012291 0.0 46-47 0.0 0.0 0.0 0.03175078461519208 0.0 48-49 0.0 0.0 0.0 0.036548165940261244 0.0 50-51 0.0 0.0 0.0 0.04069662611991043 0.0 52-53 0.0 0.0 0.0 0.05309565514847084 0.0 54-55 0.0 0.0 0.0 0.07453322870252388 0.0 56-57 0.0 0.0 0.0 0.10623766180732883 0.0 58-59 0.0 0.0 0.0 0.14306393680991292 0.0 60-61 0.0 0.0 0.0 0.18830301094776325 0.0 62-63 0.0 0.0 0.0 0.2169018928566297 0.0 64-65 0.0 0.0 0.0 0.25618479790973225 0.0 66-67 0.0 0.0 0.0 0.3217721851075378 0.0 68-69 0.0 0.0 0.0 0.41109154562355993 0.0 70-71 0.0 0.0 0.0 0.5037250391322626 0.0 72-73 0.0 0.0 0.0 0.6105420948194307 0.0 74-75 0.0 0.0 0.0 0.7178921928760509 0.0 76-77 0.0 0.0 0.0 0.8238285698658634 0.0 78-79 0.0 0.0 0.0 0.930019880162805 0.0 80-81 0.0 0.0 0.0 1.040521880925751 0.0 82-83 0.0 0.0 0.0 1.1840029813291482 0.0 84-85 0.0 0.0 0.0 1.3943229597108036 0.0 86-87 0.0 0.0 0.0 1.635327637968747 0.0 88-89 0.0 0.0 0.0 1.906762082795849 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 6240 0.0 46.306522 1 GTATCAA 10565 0.0 38.505913 1 ATCAACG 13565 0.0 29.868319 3 TCAACGC 13630 0.0 29.795576 4 CAACGCA 13755 0.0 29.490273 5 TATCAAC 13850 0.0 29.322289 2 AACGCAG 14155 0.0 28.72337 6 ACGCAGA 16095 0.0 25.11541 7 GTACATG 10470 0.0 25.010828 1 CGCAGAG 16300 0.0 24.799541 8 TACATGG 11040 0.0 23.319098 2 GAGTACT 9410 0.0 23.09421 12-13 GCAGAGT 17415 0.0 22.993534 9 ACATGGG 10915 0.0 22.75933 3 AGTACTT 9895 0.0 22.202282 12-13 GTACTTT 9915 0.0 22.012241 14-15 ATGGGAG 2205 0.0 21.972178 5 AGAGTAC 15360 0.0 21.18366 10-11 CATGGGA 5995 0.0 20.124544 4 TACTTTT 10935 0.0 19.958971 14-15 >>END_MODULE