FastQCFastQC Report
Thu 26 May 2016
SRR937616_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937616_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1990375
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT81800.4109778308107769No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61420.3085850656283364No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39610.19900772467499844No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN28040.1408779752559191No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA49600.049.091691
GTATCAA88550.041.5692441
ATCAACG111450.032.7569733
TATCAAC112800.032.5756452
TCAACGC112950.032.3656734
CAACGCA115450.031.7463675
AACGCAG118200.031.0245296
ACGCAGA135150.026.887527
CGCAGAG136950.026.568818
GTACATG103450.026.5255891
TACATGG107400.025.2286052
ACATGGG107750.024.352553
GCAGAGT147050.024.2917149
GAGTACT71800.023.94609812-13
GTACTTT76350.022.42461814-15
AGTACTT75250.021.93304612-13
CATGGGG48650.021.6927534
AGAGTAC131400.021.0550710-11
CAGAGTA143750.020.51822910-11
CATGGGA59850.020.0955964
Produced by FastQC (version 0.11.5)