##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937616_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1990375 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.7869878791685 33.0 31.0 34.0 30.0 34.0 2 31.89325830559568 33.0 31.0 34.0 30.0 34.0 3 31.936718959994977 33.0 31.0 34.0 30.0 34.0 4 35.562094077749165 37.0 35.0 37.0 33.0 37.0 5 35.56494178232745 37.0 35.0 37.0 33.0 37.0 6 35.63019280286378 37.0 35.0 37.0 33.0 37.0 7 35.54435671669911 37.0 35.0 37.0 33.0 37.0 8 35.54328154242291 37.0 35.0 37.0 33.0 37.0 9 37.1175607611631 39.0 37.0 39.0 34.0 39.0 10-11 37.03364240406958 39.0 37.0 39.0 33.0 39.0 12-13 36.92401130440244 39.0 37.0 39.0 33.0 39.0 14-15 38.05031237832067 40.0 38.0 41.0 33.0 41.0 16-17 37.954856496891296 40.0 38.0 41.0 33.0 41.0 18-19 37.860405702443 40.0 38.0 41.0 33.0 41.0 20-21 37.86526056647617 40.0 38.0 41.0 33.0 41.0 22-23 37.87191559379514 40.0 38.0 41.0 33.0 41.0 24-25 37.78715443069773 40.0 38.0 41.0 33.0 41.0 26-27 37.68497896125102 40.0 37.5 41.0 32.5 41.0 28-29 37.52918696225586 40.0 37.0 41.0 32.0 41.0 30-31 37.28516184136155 39.5 37.0 41.0 31.5 41.0 32-33 37.15487131821893 39.0 36.5 41.0 31.0 41.0 34-35 37.128069584877224 40.0 36.5 41.0 31.0 41.0 36-37 36.9572520253721 39.5 36.0 41.0 31.0 41.0 38-39 36.78434490987879 39.0 36.0 41.0 30.5 41.0 40-41 36.533752433586635 39.0 35.5 41.0 30.0 41.0 42-43 36.24642642718081 39.0 35.0 41.0 30.0 41.0 44-45 35.968137913709725 39.0 35.0 40.0 29.0 41.0 46-47 35.575889468065064 39.0 35.0 40.0 27.5 41.0 48-49 35.18693889342461 38.0 35.0 40.0 26.0 41.0 50-51 34.28831727689506 37.5 33.5 39.5 25.0 40.5 52-53 34.376438610814546 38.0 34.0 39.5 25.0 40.5 54-55 34.60295823651322 38.0 34.0 40.0 25.0 41.0 56-57 34.33396169063619 38.0 34.0 40.0 23.5 41.0 58-59 34.012199711109716 38.0 33.5 40.0 23.0 41.0 60-61 33.61836061043773 37.0 33.0 40.0 22.0 41.0 62-63 33.20531080826477 36.5 32.5 39.5 21.0 41.0 64-65 32.73759467437041 36.0 32.0 39.0 19.5 40.5 66-67 32.20778044338378 35.5 31.0 39.0 17.5 40.0 68-69 31.711678954970797 35.0 31.0 38.0 13.5 40.0 70-71 31.21846034038812 35.0 30.5 37.5 9.5 40.0 72-73 30.71716008289895 35.0 30.0 37.0 7.0 39.0 74-75 30.15129485649689 34.0 29.5 36.0 4.0 39.0 76-77 29.593835081328898 34.0 29.0 36.0 2.0 38.5 78-79 29.055588017333417 34.0 28.5 35.0 2.0 37.0 80-81 28.489439678452552 34.0 27.5 35.0 2.0 37.0 82-83 27.919547321484643 33.0 26.5 35.0 2.0 36.0 84-85 27.3531276769453 33.0 26.0 35.0 2.0 36.0 86-87 26.745275387803808 32.5 25.0 35.0 2.0 35.5 88-89 26.160981222131507 32.0 24.0 34.5 2.0 35.0 90-91 25.544336117565784 32.0 21.5 34.0 2.0 35.0 92-93 24.81565860704641 31.0 19.0 34.0 2.0 35.0 94-95 23.9702588708158 31.0 11.0 34.0 2.0 35.0 96-97 22.99099290334736 30.5 2.0 34.0 2.0 35.0 98-99 21.70982026000126 30.0 2.0 34.0 2.0 35.0 100-101 18.47902706776361 24.0 2.0 32.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.1117975099746964 1101 2 0.13766231150005837 1101 3 0.13563367376411506 1101 4 0.09310752358300789 1101 5 0.07698772803319542 1101 6 0.2128872766796306 1101 7 0.20239122451687308 1101 8 0.1682194499262195 1101 9 0.3404546421784076 1101 10-11 0.27630735147804586 1101 12-13 0.2922137102717244 1101 14-15 0.4953360462066527 1101 16-17 0.4628035942476245 1101 18-19 0.5902536951972621 1101 20-21 0.46256375981432996 1101 22-23 0.4971142452286088 1101 24-25 0.4609888988042101 1101 26-27 0.5637062584247161 1101 28-29 0.4660369526440604 1101 30-31 0.43574352581902076 1101 32-33 0.47701015432188143 1101 34-35 0.5079807996925325 1101 36-37 0.5940068791612987 1101 38-39 0.5078138034684443 1101 40-41 0.3963993159425243 1101 42-43 0.34582614023170777 1101 44-45 0.6028400722923806 1101 46-47 0.515986602789873 1101 48-49 0.6785511892877878 1101 50-51 0.5766892095295688 1101 52-53 0.7567358323559787 1101 54-55 0.8003988834222397 1101 56-57 0.8389685429893916 1101 58-59 0.8837653173868389 1101 60-61 0.9444873916951977 1101 62-63 0.8693087386250582 1101 64-65 0.9842228700441993 1101 66-67 1.0023327186931041 1101 68-69 0.9882519401558199 1101 70-71 1.016903677995444 1101 72-73 1.160576378354346 1101 74-75 1.2855100525561944 1101 76-77 1.1898331570264524 1101 78-79 1.4075179556219517 1101 80-81 1.5426133086787353 1101 82-83 1.5302191683391904 1101 84-85 1.7007049568658523 1101 86-87 1.9418674007685013 1101 88-89 1.9084143763783068 1101 90-91 2.0262789357628925 1101 92-93 2.1366727585738374 1101 94-95 2.5487701181221603 1101 96-97 2.7909565693494507 1101 98-99 2.9636789842537645 1101 100-101 3.5408691300646105 1102 1 0.06089935987368733 1102 2 -0.014967645952090436 1102 3 0.014796512983437538 1102 4 0.028973305245074243 1102 5 -0.008647448110728817 1102 6 0.06827186323688039 1102 7 -0.015996166607870066 1102 8 0.0324630882293917 1102 9 0.17845274051083493 1102 10-11 0.2024471318886043 1102 12-13 0.2516586011306501 1102 14-15 0.2683055236576877 1102 16-17 0.2577749385052428 1102 18-19 0.135117950819037 1102 20-21 0.180359276515361 1102 22-23 0.2084686473885995 1102 24-25 0.23642468309828502 1102 26-27 0.20977755439984236 1102 28-29 0.269207604213598 1102 30-31 0.2916374098051051 1102 32-33 0.25111283216963187 1102 34-35 0.18137222597807323 1102 36-37 0.38785232204416076 1102 38-39 0.4438513844984797 1102 40-41 0.3934158679455493 1102 42-43 0.2988506152686199 1102 44-45 0.3487903134433594 1102 46-47 0.4653378177828955 1102 48-49 0.354809170278088 1102 50-51 0.47417586212400664 1102 52-53 0.4437483230524535 1102 54-55 0.49766573740867415 1102 56-57 0.47439557717234493 1102 58-59 0.5229423058147304 1102 60-61 0.6892234895639788 1102 62-63 0.768197457082394 1102 64-65 0.8734328674819167 1102 66-67 0.9720534341454155 1102 68-69 0.9721187048981079 1102 70-71 0.7152385858839665 1102 72-73 0.6316355383436765 1102 74-75 0.6810708527555995 1102 76-77 0.7578946849492425 1102 78-79 1.1541914535921656 1102 80-81 1.3242804968078659 1102 82-83 1.380291047364544 1102 84-85 1.719027680834703 1102 86-87 1.5932467198285778 1102 88-89 1.7952885778435181 1102 90-91 1.7556633384283629 1102 92-93 1.8272794184193657 1102 94-95 1.9589741726308212 1102 96-97 1.7957799155871648 1102 98-99 1.9642739090150094 1102 100-101 2.6086269315207566 1103 1 0.13306647266432492 1103 2 -0.04753141543720929 1103 3 0.10116953447986177 1103 4 0.051466482172592976 1103 5 0.12684349444799636 1103 6 0.1661248447695982 1103 7 0.12813655339873264 1103 8 0.12116232756928014 1103 9 0.1771082765719001 1103 10-11 0.19899412298862273 1103 12-13 0.23380368420718156 1103 14-15 0.39222184542178695 1103 16-17 0.29984801379897164 1103 18-19 0.30951528513459436 1103 20-21 0.3224018448023216 1103 22-23 0.173691479750957 1103 24-25 0.23657663236011928 1103 26-27 0.24872518450683856 1103 28-29 0.2252034728806933 1103 30-31 0.22473509877046638 1103 32-33 0.3136830076575592 1103 34-35 0.34113018029348297 1103 36-37 0.2865815950870356 1103 38-39 0.41409217428786604 1103 40-41 0.39029592901579946 1103 42-43 0.397982688582907 1103 44-45 0.4942319996045086 1103 46-47 0.45638042637096987 1103 48-49 0.41449207548236444 1103 50-51 0.44681491971524423 1103 52-53 0.5187293674699092 1103 54-55 0.5449553468440129 1103 56-57 0.7623389597292771 1103 58-59 0.6189420218324599 1103 60-61 0.7514596578464321 1103 62-63 1.028967812894372 1103 64-65 0.8119836253303205 1103 66-67 1.0760382771772612 1103 68-69 1.0261699979078287 1103 70-71 1.0915564557732225 1103 72-73 0.9968547857812773 1103 74-75 1.1902197916178707 1103 76-77 1.160116096462989 1103 78-79 1.294812587496839 1103 80-81 1.4605054117069116 1103 82-83 1.625452687638667 1103 84-85 1.7986465166385912 1103 86-87 1.8668979680384616 1103 88-89 2.087539493522339 1103 90-91 2.068638399536102 1103 92-93 2.1018306467598613 1103 94-95 2.4160193932934853 1103 96-97 2.566288965530557 1103 98-99 2.8346274482026885 1103 100-101 3.4964779495456604 1104 1 0.039255434101903575 1104 2 0.14097310490432946 1104 3 0.07867371001580636 1104 4 0.20152019610752347 1104 5 0.16541239178761202 1104 6 0.22757398494801606 1104 7 0.2311606778316957 1104 8 0.2516425939266185 1104 9 0.40901246047884143 1104 10-11 0.3190330581173271 1104 12-13 0.35121584348743085 1104 14-15 0.41437485738833857 1104 16-17 0.4743646132982988 1104 18-19 0.4750198523138778 1104 20-21 0.3367863917317564 1104 22-23 0.33527712208591254 1104 24-25 0.38051664914198824 1104 26-27 0.2405090202996547 1104 28-29 0.3552420193557069 1104 30-31 0.4374929804682637 1104 32-33 0.34796612593584797 1104 34-35 0.6333570314809691 1104 36-37 0.5070029305808745 1104 38-39 0.6087652019201428 1104 40-41 0.6508489981841947 1104 42-43 0.6225306046018204 1104 44-45 0.6149659023653484 1104 46-47 0.59018268534588 1104 48-49 0.6891474193711318 1104 50-51 0.49320397801728433 1104 52-53 0.4147534151686685 1104 54-55 0.6491808098401961 1104 56-57 0.7388043585876289 1104 58-59 0.6999565126363123 1104 60-61 0.8547976948861304 1104 62-63 0.682021759024984 1104 64-65 0.8455694091750843 1104 66-67 0.9790965832051768 1104 68-69 0.9371812549135612 1104 70-71 0.9325867518276851 1104 72-73 1.1155857517152832 1104 74-75 1.102029408106354 1104 76-77 1.1824496081784055 1104 78-79 1.3671970975540013 1104 80-81 1.4682367553325903 1104 82-83 1.5286541228670387 1104 84-85 1.7061725962986465 1104 86-87 1.866313617229661 1104 88-89 2.0998567017131613 1104 90-91 2.1448064284838253 1104 92-93 2.4013001593681835 1104 94-95 2.7859171923176653 1104 96-97 2.797307218302951 1104 98-99 3.1316793354652113 1104 100-101 3.7686781729717147 1105 1 0.36577758377543645 1105 2 0.3234352512294585 1105 3 0.18927032813065736 1105 4 0.1945220377281487 1105 5 0.1384165103210151 1105 6 0.23871532096007542 1105 7 0.23102861689079646 1105 8 0.2582472482041993 1105 9 0.32265907022269147 1105 10-11 0.32895999600449244 1105 12-13 0.4124326524611419 1105 14-15 0.4948893057392496 1105 16-17 0.45254602967198565 1105 18-19 0.47326488830918834 1105 20-21 0.4845498606753367 1105 22-23 0.45007759086206534 1105 24-25 0.5078603625188478 1105 26-27 0.570793835300492 1105 28-29 0.5820002982775208 1105 30-31 0.5156307336911041 1105 32-33 0.616975467975017 1105 34-35 0.6908504183887132 1105 36-37 0.675959769690202 1105 38-39 0.7177989203196091 1105 40-41 0.7566495004443681 1105 42-43 0.8232533235035362 1105 44-45 0.9080506503981667 1105 46-47 0.7774351882757315 1105 48-49 0.7734738215141093 1105 50-51 0.830256983207299 1105 52-53 0.7327075420730793 1105 54-55 0.8236394738281376 1105 56-57 0.8003679279832454 1105 58-59 0.9546813075467426 1105 60-61 1.1066850546718356 1105 62-63 1.2194848763864385 1105 64-65 1.0686784666001685 1105 66-67 1.3367414517804335 1105 68-69 1.3284334899713173 1105 70-71 1.421503677995446 1105 72-73 1.4099206587971516 1105 74-75 1.368368601141679 1105 76-77 1.3820085567804483 1105 78-79 1.6047768732111258 1105 80-81 1.6426478720243693 1105 82-83 1.7144903860513665 1105 84-85 1.8243361991782727 1105 86-87 2.0481023331178214 1105 88-89 2.080197033204879 1105 90-91 2.0474165741392767 1105 92-93 2.3896786626328783 1105 94-95 2.4045586790077706 1105 96-97 2.5074619814035692 1105 98-99 2.8278151466153894 1105 100-101 3.625652155894869 1106 1 0.03600530326743012 1106 2 -0.05936879809872053 1106 3 0.04745814333404397 1106 4 0.11276242057372343 1106 5 0.040528492651219494 1106 6 0.016279981368690244 1106 7 0.030506768924659866 1106 8 0.05349083370024488 1106 9 0.07833085376769588 1106 10-11 -0.2847277639807828 1106 12-13 -0.2845318238870931 1106 14-15 -0.43860393924511243 1106 16-17 -0.1254457980475081 1106 18-19 0.08657804862577478 1106 20-21 0.11718096687817336 1106 22-23 0.07666194573046425 1106 24-25 -0.18192502308762926 1106 26-27 -5.507625275953387E-4 1106 28-29 0.20827101427236272 1106 30-31 0.16213307125782705 1106 32-33 0.27100661098992873 1106 34-35 0.2561793342731278 1106 36-37 0.12050915308795851 1106 38-39 -0.13098193740172093 1106 40-41 0.10859604931240341 1106 42-43 0.40428538682030535 1106 44-45 0.5474765292864419 1106 46-47 0.6459897253638971 1106 48-49 0.49769366322219355 1106 50-51 0.33088838390673203 1106 52-53 0.43362048151721666 1106 54-55 0.6362892805647462 1106 56-57 0.31682233622915135 1106 58-59 0.024275417614845196 1106 60-61 0.33611866409572855 1106 62-63 0.212286054372818 1106 64-65 0.49896773588049115 1106 66-67 0.6515824604312712 1106 68-69 0.38605513547104664 1106 70-71 0.35159445657081534 1106 72-73 0.3980836626624189 1106 74-75 0.588555606479428 1106 76-77 0.920978260443249 1106 78-79 0.992804850387639 1106 80-81 0.988237859140142 1106 82-83 1.0695428432573308 1106 84-85 1.2181121608937175 1106 86-87 1.3445676377377396 1106 88-89 1.3424777620839485 1106 90-91 1.727133415663296 1106 92-93 1.8322001424782641 1106 94-95 1.9484464758051203 1106 96-97 2.0275535698445815 1106 98-99 2.2520921206628763 1106 100-101 2.5276735805221477 1107 1 0.24365237369140047 1107 2 0.2486870719577503 1107 3 0.2671067426964804 1107 4 0.32988618338641373 1107 5 0.2918761741865623 1107 6 0.27992036297688117 1107 7 0.35041713229695404 1107 8 0.3226971081481764 1107 9 0.44502377610504595 1107 10-11 0.43449248900169124 1107 12-13 0.40719595778328 1107 14-15 0.4758761404731402 1107 16-17 0.6228376263106412 1107 18-19 0.7010739919506506 1107 20-21 0.5208291323840228 1107 22-23 0.5292441174727145 1107 24-25 0.46590377988579945 1107 26-27 0.6017684851604344 1107 28-29 0.606622707241101 1107 30-31 0.5855398373325613 1107 32-33 0.6866842915044273 1107 34-35 0.7440976172682738 1107 36-37 0.6098629909787192 1107 38-39 0.824996119199163 1107 40-41 0.8754587441418451 1107 42-43 0.9303122870889808 1107 44-45 0.9884692778491413 1107 46-47 1.0135407905166218 1107 48-49 0.8416337654917001 1107 50-51 0.8299848543557644 1107 52-53 0.9213174860506683 1107 54-55 1.0330214066012502 1107 56-57 0.8451324938095723 1107 58-59 0.7484500750537464 1107 60-61 1.0496330938875147 1107 62-63 1.064930114481676 1107 64-65 0.910300035227614 1107 66-67 1.124678566626372 1107 68-69 1.0888742462738463 1107 70-71 1.1844456948021644 1107 72-73 1.3664567932509293 1107 74-75 1.4553195815338391 1107 76-77 1.5214752234471156 1107 78-79 1.5745744922587441 1107 80-81 1.624823222164423 1107 82-83 1.8849210583202733 1107 84-85 2.010685498898159 1107 86-87 2.2813033135099836 1107 88-89 2.244475184465383 1107 90-91 2.443215313635072 1107 92-93 2.6228244609522022 1107 94-95 2.8709826285876012 1107 96-97 2.9874490962495095 1107 98-99 3.2619004407330365 1107 100-101 3.640721063457896 1108 1 -0.0212955620046138 1108 2 0.11234710118683111 1108 3 0.2165221610377337 1108 4 0.13996108291144083 1108 5 0.1805362034156417 1108 6 0.21686775062759267 1108 7 0.21373006616615697 1108 8 0.30199012970462746 1108 9 0.3941925740590051 1108 10-11 0.4100907717930653 1108 12-13 0.33890494572627716 1108 14-15 0.37261070386371387 1108 16-17 0.46830255141111365 1108 18-19 0.46111007159990436 1108 20-21 0.3387091104622115 1108 22-23 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-1.6240362468707836 2316 70-71 -1.7413200062150835 2316 72-73 -1.7460832885712705 2316 74-75 -1.8525042058758636 2316 76-77 -1.761419513394987 2316 78-79 -2.0458437408239583 2316 80-81 -2.103574057800195 2316 82-83 -2.331288122720565 2316 84-85 -2.4058752043304956 2316 86-87 -2.566816526531298 2316 88-89 -2.6929534053916164 2316 90-91 -2.896561057439669 2316 92-93 -3.013601161928527 2316 94-95 -2.953968075378196 2316 96-97 -3.0400967905262526 2316 98-99 -3.3644379235600503 2316 100-101 -3.617348282701622 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 7264.0 3 1430.0 4 1445.0 5 1693.0 6 1946.0 7 2271.0 8 2773.0 9 3375.0 10 4360.0 11 5589.0 12 7300.0 13 9399.0 14 10864.0 15 13717.0 16 17170.0 17 22532.0 18 19522.0 19 14729.0 20 15125.0 21 15998.0 22 18040.0 23 19928.0 24 22585.0 25 25885.0 26 29679.0 27 35444.0 28 41360.0 29 50070.0 30 60092.0 31 74860.0 32 94162.0 33 120989.0 34 160433.0 35 219557.0 36 297010.0 37 342545.0 38 187549.0 39 11685.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.914659240880006 18.567356852133216 14.706021722845685 22.811962184141095 2 15.239964750855153 19.52326992753167 40.54926390454314 24.687501417070038 3 19.1193298482081 24.27587075644013 29.325993575612518 27.278805819739244 4 12.779375806467053 16.021467831758063 36.30667438557351 34.89248197620138 5 14.064851525306224 37.40558629831782 33.22238731446646 15.307174861909493 6 32.76735519173005 35.205203159032074 17.074652926374174 14.95278872286371 7 29.468230086758034 32.748653420455526 19.77245461351065 18.010661879275787 8 27.038404046272753 34.66378145857741 19.080284540054162 19.217529955095678 9 27.080156178805098 16.639537606013537 19.888932803339067 36.391373411842295 10-11 25.630675627503617 26.554157746444833 26.12125426207024 21.6939123639813 12-13 26.27676897266357 23.762605308579566 27.26606037658328 22.694565342173583 14-15 23.082771197286846 24.91873823743539 25.6470480431221 26.35144252215566 16-17 22.637698947705463 28.593191651320076 26.006783377955077 22.762326023019384 18-19 22.616545274646665 26.699817591049047 28.72192161927398 21.9617155150303 20-21 23.270605836667546 25.79759332607089 28.837096446168996 22.094704391092566 22-23 23.183451974270955 25.550019621756963 28.88068385913517 22.38584454483691 24-25 22.794530575278344 25.850736891229975 28.872457870623602 22.482274662868075 26-27 22.386461011139673 26.24952548390572 28.774580437148895 22.589433067805707 28-29 22.316723463912876 26.32770748155296 28.990385208431253 22.36518384610292 30-31 22.377645440207043 26.050906747327968 28.82891319972861 22.742534612736378 32-33 22.161999412932502 26.212437875822637 28.743323114196713 22.882239597048148 34-35 22.886190476789867 26.100604310606336 28.585439903129046 22.427765309474747 36-37 22.79834429651229 25.86034859989146 28.425032698230822 22.91627440536543 38-39 22.43505119817291 26.24410062593583 28.569468960061574 22.75137921582969 40-41 22.649527690723247 26.089563087492234 28.6917189077741 22.569190314010417 42-43 22.593734787399903 26.078530871226707 28.349717004735638 22.978017336637755 44-45 22.497155658477645 26.316728755537653 28.227572492952074 22.95854309303262 46-47 22.71491984793738 26.540474765227785 27.748834886398594 22.99577050043624 48-49 22.877152402657742 26.71734663025738 27.38910747493799 23.01639349214689 50-51 22.848211422268072 26.847092727324906 27.333513573112654 22.971182277294368 52-53 23.168908894397987 26.694724619758674 27.203936941861024 22.932429543982312 54-55 22.893226783738644 26.733216110318857 27.296994198845308 23.076562907097195 56-57 22.847378781907345 27.075257873620405 27.184482634521366 22.89288070995088 58-59 22.99030357473117 26.529907690836644 27.246640472353175 23.233148262079013 60-61 22.965839103324278 26.593307750076008 27.55293157149789 22.887921575101828 62-63 22.882504361761054 26.81280778631913 27.54108913544649 22.763598716473325 64-65 22.651282832108745 26.731933453045038 27.502727488935896 23.114056225910325 66-67 22.715399574323616 26.88163735160532 27.313957851645466 23.0890052224256 68-69 22.631825578463165 26.693512545217796 27.547585959744776 23.127075916574263 70-71 22.65804275881161 26.816343479847486 27.40620871031471 23.119405051026188 72-73 22.869890145072134 26.75858243197054 27.101995490627118 23.26953193233021 74-75 22.605031165700133 27.02788488815311 27.194316749635085 23.172767196511675 76-77 22.905528538778306 26.72982048106644 27.174439443823562 23.19021153633169 78-79 22.830176284136694 26.956821373278018 27.086598635123778 23.126403707461513 80-81 22.708181528730556 27.192511518272553 27.112044067067114 22.987262885929773 82-83 22.856959499080155 27.123158734512987 26.968257124089906 23.051624642316956 84-85 22.745278838025783 26.942174841222844 26.94373511916371 23.368811201587658 86-87 22.913500346423433 26.93233933842562 26.969586463790634 23.184573851360312 88-89 22.797883382681473 26.939855006075813 26.875900423142244 23.386361188100473 90-91 22.739341051859565 26.865147609829933 27.087914535623707 23.307596802686795 92-93 22.98283130566789 26.606304390664743 27.08080352277981 23.330060780887553 94-95 22.73471770956287 26.49752478362305 27.228965694281204 23.538791812532875 96-97 22.851210079824618 26.54544264721243 27.024415536038116 23.578931736924833 98-99 22.522139541088247 26.651187550259937 26.84237351485429 23.98429939379753 100-101 22.907323809900078 25.93323296040336 27.627611229118788 23.531832000577776 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2554.0 1 1466.5 2 370.0 3 358.0 4 320.0 5 330.0 6 513.0 7 1041.0 8 1726.5 9 2138.0 10 2086.0 11 1977.5 12 2227.5 13 2514.0 14 2778.0 15 2869.5 16 2855.5 17 3004.0 18 3118.0 19 3306.5 20 3537.5 21 3874.0 22 4448.5 23 5127.5 24 6125.0 25 7542.0 26 9255.0 27 11280.0 28 12928.0 29 15066.5 30 18470.5 31 21817.5 32 25771.0 33 29598.5 34 32753.0 35 36220.5 36 40928.5 37 45640.0 38 49270.5 39 51549.5 40 53567.5 41 57513.5 42 61040.0 43 65845.5 44 70517.0 45 72350.5 46 72606.5 47 75102.0 48 80684.5 49 81176.0 50 78464.5 51 75262.5 52 70964.0 53 70241.5 54 70220.5 55 66242.5 56 61413.0 57 57021.5 58 52659.5 59 47436.0 60 40242.0 61 32424.5 62 25590.0 63 19775.0 64 15415.5 65 12519.5 66 9563.0 67 7303.5 68 5415.0 69 3680.5 70 2657.5 71 1956.0 72 1499.5 73 1242.5 74 961.5 75 732.5 76 541.5 77 358.0 78 202.5 79 135.5 80 102.0 81 66.5 82 38.0 83 28.5 84 19.5 85 16.0 86 13.5 87 12.5 88 10.0 89 8.5 90 9.5 91 9.0 92 6.5 93 3.0 94 1.0 95 3.5 96 3.5 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.33445958676128873 2 0.2836651384789298 3 0.28889028449412796 4 0.28326320417006845 5 0.28266030270677633 6 0.2108647867864096 7 0.20327827670665075 8 0.20860390629906422 9 0.2105130942661559 10-11 0.213577843371224 12-13 0.2163913835332538 14-15 0.21106575394084032 16-17 0.20342900207247377 18-19 0.19616906361866485 20-21 0.19589273378132263 22-23 0.20395654085285436 24-25 0.2021478364629781 26-27 0.20805124662437985 28-29 0.21184450166425922 30-31 0.2156628775984425 32-33 0.21262324938767818 34-35 0.21239716133894365 36-37 0.2024241663003203 38-39 0.19240092947308923 40-41 0.18855743264460215 42-43 0.19825409784588333 44-45 0.1997111097155059 46-47 0.2053884318281731 48-49 0.21360296426552786 50-51 0.20759907052691076 52-53 0.21938076995541042 54-55 0.2076241914212146 56-57 0.18318156126358098 58-59 0.16592350687684482 60-61 0.18383470451548076 62-63 0.1761225899642027 64-65 0.18375934183256926 66-67 0.18360861646674623 68-69 0.18097092256484332 70-71 0.18338252841801167 72-73 0.19322991898511585 74-75 0.19978647239841738 76-77 0.19870627394335239 78-79 0.19566664573258807 80-81 0.19380769955410412 82-83 0.1823776926458582 84-85 0.17833322866294038 86-87 0.18310619858066945 88-89 0.19137097280663193 90-91 0.18938642215662876 92-93 0.18330716573510017 94-95 0.1848897820762419 96-97 0.19255165483891226 98-99 0.1923004458958739 100-101 0.1734095333793883 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1990375.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 44.100784207630575 #Duplication Level Percentage of deduplicated Percentage of total 1 70.08993778836039 30.910212215307336 2 14.566406895202164 12.847799343317053 3 5.797845835744426 7.670686442138234 4 2.880331973450937 5.08099595229994 5 1.6622472431743405 3.66532034854802 6 1.02116475660618 2.7020499942916043 7 0.7269499914851304 2.244134529295723 8 0.4931630428563604 1.7399101545749456 9 0.38554263974625136 1.5302459482460717 >10 2.1123044459675975 17.047156953705674 >50 0.17014823289183076 5.18169113410806 >100 0.0888208006435458 6.893745176012511 >500 0.00456564788523481 1.3501937241868172 >1k 3.424235913926108E-4 0.4149292349279325 >5k 2.282823942617405E-4 0.720928849040137 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8180 0.4109778308107769 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6142 0.3085850656283364 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3961 0.19900772467499844 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 2804 0.1408779752559191 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 1.2560447151918608E-4 0.0 24-25 0.0 0.0 0.0 1.5072536582302329E-4 0.0 26-27 0.0 0.0 0.0 2.2608804873453493E-4 0.0 28-29 0.0 0.0 0.0 6.782641462036048E-4 0.0 30-31 0.0 0.0 0.0 0.001884067072787791 0.0 32-33 0.0 0.0 0.0 0.003466683413929536 0.0 34-35 0.0 0.0 0.0 0.005124662437982793 0.0 36-37 0.0 0.0 0.0 0.007712114551278026 0.0 38-39 0.0 0.0 0.0 0.012309238208880236 0.0 40-41 0.0 0.0 0.0 0.016680273817747913 0.0 42-43 0.0 0.0 0.0 0.020649375117754193 0.0 44-45 0.0 0.0 0.0 0.023588519751303147 0.0 46-47 0.0 0.0 0.0 0.02903975381523582 0.0 48-49 0.0 0.0 0.0 0.033159580481065126 0.0 50-51 0.0 0.0 0.0 0.03775670413866734 0.0 52-53 0.0 0.0 0.0 0.04931231551843246 0.0 54-55 0.0 0.0 0.0 0.06960999811593294 0.0 56-57 0.0 0.0 0.0 0.10128744583307166 0.0 58-59 0.0 0.0 0.0 0.13527601582616341 0.0 60-61 0.0 0.0 0.0 0.1775796018338253 0.0 62-63 0.0 0.0 0.0 0.2054637945110846 0.0 64-65 0.0 0.0 0.0 0.2405576838535452 0.0 66-67 0.0 0.0 0.0 0.3077309552220059 0.0 68-69 0.0 0.0 0.0 0.39741254788670477 0.0 70-71 0.0 0.0 0.0 0.48656660177102307 0.0 72-73 0.0 0.0 0.0 0.5951391069522075 0.0 74-75 0.0 0.0 0.0 0.707881680587829 0.0 76-77 0.0 0.0 0.0 0.8123343591031841 0.0 78-79 0.0 0.0 0.0 0.9165609495698046 0.0 80-81 0.0 0.0 0.0 1.0261885323117503 0.0 82-83 0.0 0.0 0.0 1.1699805313069145 0.0 84-85 0.0 0.0 0.0 1.3798656032154746 0.0 86-87 0.0 0.0 0.0 1.6296928970671356 0.0 88-89 0.0 0.0 0.0 1.9099164730264397 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4960 0.0 49.09169 1 GTATCAA 8855 0.0 41.569244 1 ATCAACG 11145 0.0 32.756973 3 TATCAAC 11280 0.0 32.575645 2 TCAACGC 11295 0.0 32.365673 4 CAACGCA 11545 0.0 31.746367 5 AACGCAG 11820 0.0 31.024529 6 ACGCAGA 13515 0.0 26.88752 7 CGCAGAG 13695 0.0 26.56881 8 GTACATG 10345 0.0 26.525589 1 TACATGG 10740 0.0 25.228605 2 ACATGGG 10775 0.0 24.35255 3 GCAGAGT 14705 0.0 24.291714 9 GAGTACT 7180 0.0 23.946098 12-13 GTACTTT 7635 0.0 22.424618 14-15 AGTACTT 7525 0.0 21.933046 12-13 CATGGGG 4865 0.0 21.692753 4 AGAGTAC 13140 0.0 21.05507 10-11 CAGAGTA 14375 0.0 20.518229 10-11 CATGGGA 5985 0.0 20.095596 4 >>END_MODULE