FastQCFastQC Report
Thu 26 May 2016
SRR937616_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937616_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1990375
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT99470.49975507128053764No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT83380.4189160334107894No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55870.28070087295107704No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21340.10721597688877725No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA61600.045.6751171
GTATCAA100200.038.040421
ATCAACG126750.029.8344613
TCAACGC127700.029.6125144
TATCAAC129000.029.4245742
CAACGCA130000.029.0520575
AACGCAG133850.028.2462316
GTACATG98250.025.9764861
ACGCAGA150550.025.021477
CGCAGAG152150.024.6959028
TACATGG103350.024.362312
GTAAGGT6650.024.2890424
TAAGGTG7250.024.2447015
ACATGGG101250.023.8353633
GAGTACT84050.023.7938512-13
GCAGAGT163650.022.7282569
GTACTTT91250.021.96571714-15
CATGGGG44500.021.564754
AGTACTT87550.021.5404512-13
AGAGTAC142100.020.67597610-11
Produced by FastQC (version 0.11.5)