##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937616_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1990375 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.23465276643849 33.0 31.0 34.0 31.0 34.0 2 32.44905859448596 34.0 31.0 34.0 31.0 34.0 3 32.5586178483954 34.0 31.0 34.0 31.0 34.0 4 36.18179840482321 37.0 35.0 37.0 35.0 37.0 5 36.02141104063305 37.0 35.0 37.0 35.0 37.0 6 36.12040997299504 37.0 35.0 37.0 35.0 37.0 7 36.08156804622244 37.0 35.0 37.0 35.0 37.0 8 36.074161401745904 37.0 35.0 37.0 35.0 37.0 9 37.74776763172769 39.0 37.0 39.0 35.0 39.0 10-11 37.69375092633298 39.0 37.0 39.0 35.0 39.0 12-13 37.612957106072976 39.0 37.0 39.0 35.0 39.0 14-15 38.85650693964705 40.0 38.0 41.0 35.0 41.0 16-17 38.827497833322866 40.0 38.0 41.0 35.0 41.0 18-19 38.80283539534007 40.0 38.0 41.0 35.0 41.0 20-21 38.715620925704954 40.0 38.0 41.0 34.5 41.0 22-23 38.568418765308046 40.0 38.0 41.0 34.0 41.0 24-25 38.50365785341958 40.0 38.0 41.0 34.0 41.0 26-27 38.43721534886642 40.0 38.0 41.0 34.0 41.0 28-29 38.31804433837845 40.0 38.0 41.0 34.0 41.0 30-31 38.39489643911323 40.0 38.0 41.0 34.0 41.0 32-33 38.3282813540162 40.0 38.0 41.0 34.0 41.0 34-35 38.190417132449916 40.0 38.0 41.0 34.0 41.0 36-37 38.024871946241284 40.0 37.5 41.0 33.5 41.0 38-39 37.88532161024932 40.0 37.0 41.0 33.0 41.0 40-41 37.69400389373862 40.0 37.0 41.0 33.0 41.0 42-43 37.47523582239528 40.0 37.0 41.0 33.0 41.0 44-45 37.153965207561384 40.0 36.5 41.0 32.0 41.0 46-47 36.775472963637505 39.5 36.0 41.0 31.0 41.0 48-49 36.42751441311311 39.0 36.0 41.0 30.5 41.0 50-51 36.15227055203165 39.0 35.0 41.0 30.0 41.0 52-53 35.90918419895748 39.0 35.0 40.0 29.5 41.0 54-55 35.67021641650443 39.0 35.0 40.0 29.0 41.0 56-57 35.368089179174774 38.0 35.0 40.0 28.0 41.0 58-59 35.08650505557998 38.0 34.5 40.0 28.0 41.0 60-61 34.71492784023111 37.5 34.0 40.0 27.0 41.0 62-63 34.32483853545186 37.0 34.0 40.0 26.0 41.0 64-65 33.900925453746154 36.5 33.0 39.0 26.0 41.0 66-67 33.46976223073541 36.0 33.0 39.0 25.5 40.0 68-69 33.01294404320794 35.0 32.5 38.5 24.5 40.0 70-71 32.58919399610626 35.0 32.0 38.0 23.5 40.0 72-73 32.15132374552534 35.0 31.5 37.0 22.5 39.0 74-75 31.66668617722791 35.0 31.0 36.5 21.0 39.0 76-77 30.461680336619985 33.5 29.5 35.0 20.0 37.5 78-79 31.04693763737989 34.0 31.0 36.0 20.0 37.5 80-81 30.861150536959116 34.0 31.0 35.0 20.0 37.0 82-83 30.52911536770709 34.0 31.0 35.0 18.0 37.0 84-85 30.153589398982604 34.0 30.0 35.0 17.0 36.0 86-87 29.744255981912957 34.0 30.0 35.0 11.5 36.0 88-89 29.316068328832507 34.0 29.5 35.0 7.0 35.0 90-91 28.80849689128933 34.0 29.0 35.0 2.0 35.0 92-93 28.35970809520819 33.0 29.0 35.0 2.0 35.0 94-95 27.88014620360485 33.0 27.0 35.0 2.0 35.0 96-97 27.32024116058532 33.0 27.0 35.0 2.0 35.0 98-99 26.47236626263895 32.5 25.0 34.0 2.0 35.0 100-101 23.984383847264965 30.5 12.5 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.22170844398883105 1101 2 0.20274770268270714 1101 3 0.1798221177869408 1101 4 0.10851956050694866 1101 5 0.010076466826291153 1101 6 0.06704583371156758 1101 7 0.08256509016020885 1101 8 0.10616693147530043 1101 9 0.3320446153812142 1101 10-11 0.30348524876289673 1101 12-13 0.2203628414053398 1101 14-15 0.22359071294017951 1101 16-17 0.20221866089666918 1101 18-19 0.26301721905623765 1101 20-21 0.2936628614714678 1101 22-23 0.18458087763132625 1101 24-25 0.1852499506058365 1101 26-27 0.1177446472978474 1101 28-29 0.12029532440948287 1101 30-31 0.17576454554870224 1101 32-33 0.24680397265876053 1101 34-35 0.11180459377845153 1101 36-37 0.28967882166561054 1101 38-39 0.32715092659022105 1101 40-41 0.2686988619214148 1101 42-43 0.2697399209928548 1101 44-45 0.3167833793694683 1101 46-47 0.1683618605758852 1101 48-49 0.2689740230926603 1101 50-51 0.373280083127419 1101 52-53 0.3564935266696523 1101 54-55 0.4467941176993193 1101 56-57 0.47901201382436653 1101 58-59 0.4952019075505518 1101 60-61 0.365744165233977 1101 62-63 0.3767477293342836 1101 64-65 0.30140605799265785 1101 66-67 0.5216523171780878 1101 68-69 0.42542735739232995 1101 70-71 0.5133348735133367 1101 72-73 0.4632102204306676 1101 74-75 0.38799858668802756 1101 76-77 0.41007773952800974 1101 78-79 0.5787329296029675 1101 80-81 0.6962594553170938 1101 82-83 0.54824380927748 1101 84-85 0.6796439291078222 1101 86-87 0.837998047934871 1101 88-89 0.9214856533740452 1101 90-91 0.9498310183064902 1101 92-93 1.168332343912578 1101 94-95 1.1144475767828617 1101 96-97 1.364052955539588 1101 98-99 1.6803576674577378 1101 100-101 2.1509482236305217 1102 1 0.16182661406519117 1102 2 0.20543396065062325 1102 3 0.13184793410083984 1102 4 0.054657135959224945 1102 5 -0.05723859992380653 1102 6 -0.05306403944668148 1102 7 0.023396167696304815 1102 8 0.15172437630340596 1102 9 0.23042436323159876 1102 10-11 0.028848652904734706 1102 12-13 0.1493866070208938 1102 14-15 0.15053196953980574 1102 16-17 0.22390579823332502 1102 18-19 0.24699543005941393 1102 20-21 0.2553067188760352 1102 22-23 0.2621421371244068 1102 24-25 0.16083319693192522 1102 26-27 0.04941240191823937 1102 28-29 0.06198343785415972 1102 30-31 0.14968405062708 1102 32-33 0.12223505071776941 1102 34-35 0.2482614437035906 1102 36-37 0.16472512143186435 1102 38-39 0.1448107904231506 1102 40-41 0.1577541636695159 1102 42-43 0.25609009625653556 1102 44-45 0.11577495956633754 1102 46-47 0.21538771610840968 1102 48-49 0.11162497558234463 1102 50-51 0.16288795352119934 1102 52-53 0.05355786003146079 1102 54-55 0.005589264873748334 1102 56-57 0.028202955367703453 1102 58-59 0.32420761081022675 1102 60-61 0.42354479342920115 1102 62-63 0.2540175072871449 1102 64-65 0.2656079502477624 1102 66-67 0.2660603948205065 1102 68-69 0.2640228861204079 1102 70-71 0.22176748807094526 1102 72-73 0.31989642959318587 1102 74-75 0.2560441439149699 1102 76-77 0.20304032757382373 1102 78-79 0.4063490951718052 1102 80-81 0.3291637514124872 1102 82-83 0.4307686651708238 1102 84-85 0.30065681111902265 1102 86-87 0.3756896527087399 1102 88-89 0.4764928260248915 1102 90-91 0.4953629710280012 1102 92-93 0.8454390813238355 1102 94-95 0.49025134072508436 1102 96-97 0.6238813609799934 1102 98-99 0.8785340667347192 1102 100-101 1.5802592656781158 1103 1 0.13800537481527897 1103 2 0.22677504117037728 1103 3 0.15631094517521404 1103 4 0.08503035240057955 1103 5 0.05693773901044352 1103 6 -0.02173835770177135 1103 7 0.12128851310872335 1103 8 0.11617176366647897 1103 9 0.1944223463013799 1103 10-11 0.12283085293322671 1103 12-13 0.2518169282676439 1103 14-15 0.19653135752519546 1103 16-17 0.1405752766056807 1103 18-19 0.12671007473478824 1103 20-21 0.134389081233671 1103 22-23 0.22443821816901988 1103 24-25 0.22381962201683336 1103 26-27 0.18692917842887624 1103 28-29 0.17001198229035452 1103 30-31 0.12333801681912604 1103 32-33 0.08252228519902616 1103 34-35 0.23400961047148172 1103 36-37 0.22972121316094984 1103 38-39 0.11919556578661172 1103 40-41 0.17393025798537565 1103 42-43 0.24076782323616186 1103 44-45 0.19745571287852215 1103 46-47 0.297195581948813 1103 48-49 0.4011673985978419 1103 50-51 0.3141470587614634 1103 52-53 0.13119444332167518 1103 54-55 0.18975083298075646 1103 56-57 0.3270158063622972 1103 58-59 0.2283102877414933 1103 60-61 0.2323244381795604 1103 62-63 0.3461676467477446 1103 64-65 0.2292114744396727 1103 66-67 0.25739003169809394 1103 68-69 0.08440187124460863 1103 70-71 0.3018578703504531 1103 72-73 0.3628699536351476 1103 74-75 0.2314584721797388 1103 76-77 0.2284102308886382 1103 78-79 0.2908267692913675 1103 80-81 0.36740962664023513 1103 82-83 0.30709825050046646 1103 84-85 0.40693354575399 1103 86-87 0.47628931924044693 1103 88-89 0.49619608797839376 1103 90-91 0.6608531731708958 1103 92-93 0.8676263681028225 1103 94-95 0.831827540761072 1103 96-97 1.0007060408204396 1103 98-99 1.2912061196922586 1103 100-101 1.9276991607827512 1104 1 0.13941399535373478 1104 2 0.1851544648467609 1104 3 0.1182769449519867 1104 4 0.07612146279417686 1104 5 -0.06088562087092697 1104 6 0.06719109068900053 1104 7 0.08500619505844753 1104 8 0.0977839590217684 1104 9 0.21349129147530022 1104 10-11 0.16453837806616178 1104 12-13 0.1650508609249144 1104 14-15 0.17026970289683163 1104 16-17 0.10750682571954684 1104 18-19 0.0732760452130492 1104 20-21 0.18206368640137072 1104 22-23 0.2623465287810589 1104 24-25 0.12150055526407044 1104 26-27 0.22503916543389124 1104 28-29 0.13663858772910942 1104 30-31 0.24993560776409396 1104 32-33 0.2647218957480817 1104 34-35 0.26784116795635526 1104 36-37 0.31879242049873113 1104 38-39 0.16073056355434545 1104 40-41 0.263947313903671 1104 42-43 0.4400237257897217 1104 44-45 0.4001274081261812 1104 46-47 0.4938462590034902 1104 48-49 0.4733239483096341 1104 50-51 0.5134517921649504 1104 52-53 0.6100145604359923 1104 54-55 0.6396528723164963 1104 56-57 0.6321894349129948 1104 58-59 0.6868968688246184 1104 60-61 0.7284638113907533 1104 62-63 0.7296335707868735 1104 64-65 0.7497828402285833 1104 66-67 0.6336357166454363 1104 68-69 0.7190899367855792 1104 70-71 0.7325672679970054 1104 72-73 0.7588989840737028 1104 74-75 0.8239454722116335 1104 76-77 0.6260172950184888 1104 78-79 0.7591992021838259 1104 80-81 0.8534416367571929 1104 82-83 0.867164306681854 1104 84-85 0.8587697601074673 1104 86-87 0.7575860324259693 1104 88-89 0.984004071124776 1104 90-91 0.8924426970601438 1104 92-93 1.1728427236472143 1104 94-95 1.4097918474028681 1104 96-97 1.7994784681322322 1104 98-99 1.986066498340616 1104 100-101 2.7260578961235282 1105 1 0.22262283513273928 1105 2 0.263571072916406 1105 3 0.19920538961965661 1105 4 0.12946765398788074 1105 5 0.05532345329615396 1105 6 0.14498703912362032 1105 7 0.1702789892991987 1105 8 0.1363098589045748 1105 9 0.185638219317255 1105 10-11 0.1991102180125921 1105 12-13 0.09839986477557972 1105 14-15 0.28160953212837114 1105 16-17 0.19574670517710757 1105 18-19 0.2544628525125745 1105 20-21 0.23838352567811683 1105 22-23 0.2339536943594922 1105 24-25 0.21811009820731897 1105 26-27 0.1278498133495063 1105 28-29 0.19346912514749448 1105 30-31 0.2264392072953214 1105 32-33 0.1569453010720352 1105 34-35 0.1447310390429024 1105 36-37 0.19955692744666464 1105 38-39 0.1382816768977264 1105 40-41 0.23377192465203933 1105 42-43 0.2848277438710838 1105 44-45 0.32654857002137305 1105 46-47 0.3364689946472197 1105 48-49 0.31435311288355194 1105 50-51 0.46498475717415744 1105 52-53 0.5264936496708827 1105 54-55 0.531285753615677 1105 56-57 0.45338802858452 1105 58-59 0.5075416369478489 1105 60-61 0.6307180889732109 1105 62-63 0.6253414562715562 1105 64-65 0.680774569677773 1105 66-67 0.5494503491584126 1105 68-69 0.5719776648954067 1105 70-71 0.6929614417790262 1105 72-73 0.6012513028414972 1105 74-75 0.7052604563067177 1105 76-77 0.5981836435870491 1105 78-79 0.6325795470691453 1105 80-81 0.818753674259284 1105 82-83 0.917304202881418 1105 84-85 1.0640307679762095 1105 86-87 1.1567524144785395 1105 88-89 1.1074349768672818 1105 90-91 1.1959968239645455 1105 92-93 1.5615291458805984 1105 94-95 1.4107501598086927 1105 96-97 1.6192699297093291 1105 98-99 2.208258897470035 1105 100-101 2.5584094782430675 1106 1 -0.10815839070933464 1106 2 0.033674073100463886 1106 3 -0.08960329802989264 1106 4 0.006262647545767663 1106 5 -0.09292848854076396 1106 6 -0.004687026769992997 1106 7 -0.07470948852152759 1106 8 -0.05279730104388847 1106 9 0.014589885063621466 1106 10-11 -0.01310211403232131 1106 12-13 -0.15859730069469435 1106 14-15 6.986541603950513E-4 1106 16-17 -0.0203756581580663 1106 18-19 0.05074394951238759 1106 20-21 -5.942766410456102E-4 1106 22-23 0.029627981125550207 1106 24-25 -0.019240315928527707 1106 26-27 -0.07447818223304381 1106 28-29 0.1141529462408144 1106 30-31 -6.500619850058342E-4 1106 32-33 -0.015750888878265812 1106 34-35 -0.2288060859340888 1106 36-37 -0.03786887781745918 1106 38-39 -0.005040391940852373 1106 40-41 -0.059070041112725846 1106 42-43 0.014203337465779953 1106 44-45 0.07700216840164842 1106 46-47 0.060065976057124715 1106 48-49 0.1189157486280763 1106 50-51 0.31568705792880536 1106 52-53 0.22799896901562988 1106 54-55 0.08886219388809025 1106 56-57 -0.005824782017363361 1106 58-59 0.1324385263275616 1106 60-61 0.23959701406063516 1106 62-63 0.2893710531793374 1106 64-65 0.2517140874395949 1106 66-67 0.30734160629075546 1106 68-69 -0.0024317589956339702 1106 70-71 0.28322052515837015 1106 72-73 0.41999262439496476 1106 74-75 0.4598191899713626 1106 76-77 0.485678287430229 1106 78-79 0.4608113158893161 1106 80-81 0.5093509869778536 1106 82-83 0.44865012594418374 1106 84-85 0.5022777769951681 1106 86-87 0.6025214555240019 1106 88-89 0.5999236939664918 1106 90-91 0.8179911549050942 1106 92-93 1.0899473310453338 1106 94-95 0.9372173050323234 1106 96-97 1.2695731875779082 1106 98-99 1.418500163805394 1106 100-101 2.1396066069012676 1107 1 0.15399510404030536 1107 2 0.18910580680996247 1107 3 0.08508662211265516 1107 4 0.0876855811587518 1107 5 -0.11935777919683943 1107 6 0.007264910272084535 1107 7 0.09730391927119086 1107 8 0.07135383649561078 1107 9 0.20539340139008289 1107 10-11 0.17129607235433042 1107 12-13 0.12376779194644172 1107 14-15 0.21002633885105837 1107 16-17 0.2401025875300533 1107 18-19 0.29414996735850707 1107 20-21 0.31570327357727734 1107 22-23 0.23743716774884405 1107 24-25 0.27590295535017617 1107 26-27 0.1062510738537128 1107 28-29 0.12930820077774996 1107 30-31 0.1738894874073651 1107 32-33 0.19056967081994003 1107 34-35 0.20284714548548521 1107 36-37 0.3020251347295826 1107 38-39 0.19936304944673822 1107 40-41 0.30422612633271484 1107 42-43 0.35349833210637627 1107 44-45 0.16051593696815303 1107 46-47 0.26114574534750545 1107 48-49 0.24561767870988405 1107 50-51 0.31713783840665855 1107 52-53 0.29281325751401965 1107 54-55 0.20404821860167033 1107 56-57 0.2477674403492287 1107 58-59 0.29149597868453725 1107 60-61 0.49883937748861484 1107 62-63 0.3670885150950838 1107 64-65 0.4654247097337887 1107 66-67 0.44704922871023456 1107 68-69 0.4067203819822396 1107 70-71 0.4855500972412088 1107 72-73 0.575040238415724 1107 74-75 0.5739975711526597 1107 76-77 0.4944437276206628 1107 78-79 0.5943836086937928 1107 80-81 0.6369737022704882 1107 82-83 0.6522925782315596 1107 84-85 0.49422054388657344 1107 86-87 0.6847539550947879 1107 88-89 0.7788174698644816 1107 90-91 0.8412336586984388 1107 92-93 1.0477588377573497 1107 94-95 1.2088846135902038 1107 96-97 1.4078459801294976 1107 98-99 1.8812650599350214 1107 100-101 2.6017771253018935 1108 1 0.04515787349590994 1108 2 0.12875231758818728 1108 3 0.11853565389928633 1108 4 0.0759665457184866 1108 5 -0.06968660637988933 1108 6 0.016124976037517058 1108 7 0.12211667045862384 1108 8 0.02241795779629996 1108 9 0.23791989024649496 1108 10-11 0.13684457436382758 1108 12-13 0.14278724755477157 1108 14-15 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sequence quality scores pass #Quality Count 2 4.0 3 6.0 4 11.0 5 40.0 6 71.0 7 167.0 8 414.0 9 795.0 10 1402.0 11 2304.0 12 3813.0 13 5350.0 14 7160.0 15 10233.0 16 14337.0 17 20312.0 18 18351.0 19 10898.0 20 9430.0 21 9409.0 22 9533.0 23 10371.0 24 12235.0 25 14565.0 26 17969.0 27 22091.0 28 27939.0 29 35172.0 30 45219.0 31 57333.0 32 74751.0 33 99966.0 34 140535.0 35 208345.0 36 323268.0 37 460383.0 38 298460.0 39 17730.0 40 3.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.18214373491232 19.10042163809087 15.271601978467256 22.445832648529542 2 15.758990140048986 20.03572191170006 39.81129184198958 24.393996106261383 3 19.493361803680212 24.261759718645983 29.859498838158636 26.385379639515165 4 13.332311750298311 16.59626954719588 35.95915342586196 34.112265276643846 5 14.037524383613409 37.51780091715551 33.245178803115074 15.199495896116009 6 33.00302706776361 35.583746781385415 16.594862777114866 14.818363373736105 7 29.568548640331592 32.94233498712554 19.558022985618287 17.931093386924573 8 27.39956038434968 34.62764554418138 18.787565157319598 19.185228914149345 9 26.992501413050306 16.49020913144508 19.881278653520067 36.636010801984554 10-11 25.748263518181247 26.71690008164291 25.941367832694844 21.593468567481004 12-13 26.427931177937708 23.754755583602808 27.301797927138328 22.515515311321153 14-15 23.10147585254035 24.912566727375495 25.779865603215473 26.206091816868682 16-17 22.6199836714187 28.489932801607736 26.340187150662565 22.549896376310997 18-19 22.58021729573573 26.365257803177794 29.22494504804371 21.82957985304277 20-21 23.15005198772994 25.418922629345698 29.56618443724256 21.864840945681802 22-23 23.12989544657526 25.220712731803633 29.576184327537092 22.073207494084016 24-25 22.83679915231692 25.516079576299138 29.555586873070865 22.091534398313076 26-27 22.476373502147865 25.967267063581783 29.378325420152233 22.17803401411812 28-29 22.347218213256543 25.947348945313355 29.593301213039748 22.11213162839036 30-31 22.334130589385833 25.7508848235892 29.490142152068827 22.42484243495614 32-33 22.194812535326257 25.834679394586445 29.437141242228222 22.533366827859073 34-35 22.85102053633109 25.71824404948816 29.281441939333043 22.149293474847703 36-37 22.784600891791747 25.477158826854236 29.06090560823965 22.677334673114363 38-39 22.476618727626704 25.82860013816492 29.256094956980466 22.43868617722791 40-41 22.643572191170005 25.785015386547762 29.26526408340137 22.306148338880863 42-43 22.602763645060968 25.74725719495629 28.939158957702677 22.710820202280065 44-45 22.548514727124285 25.975205677322112 28.786937134962002 22.689342460591597 46-47 22.74870313383156 26.326194812535324 28.259272750109904 22.665829303523203 48-49 23.047239841738364 26.454838912265277 27.772027884192678 22.72589336180368 50-51 22.96391383533254 26.59561640394398 27.773535137850907 22.666934622872574 52-53 23.323480913637336 26.52122163975213 27.522566110690754 22.632731335919782 54-55 22.976650128744584 26.578609558500283 27.640067826414622 22.804672486340515 56-57 22.959844250455316 26.900533819003957 27.472260252464984 22.66736167807574 58-59 23.22258368397915 26.33074169440432 27.536243170256864 22.91043145135967 60-61 23.034478427432017 26.41446963511901 28.009897632355713 22.54115430509326 62-63 22.920354204609684 26.61232179865603 27.946819066758778 22.52050492997551 64-65 22.83228034918043 26.477397475350124 27.860026376939018 22.83029579853043 66-67 22.80386861772279 26.654424417509265 27.692269044777994 22.849437919989953 68-69 22.859762607548827 26.412434842680398 27.902304842052377 22.825497707718394 70-71 22.864334610312127 26.544068328832505 27.731181310054637 22.860415750800726 72-73 23.01183094232541 26.539132846389673 27.39985048036198 23.049185730922932 74-75 22.85291034425933 26.803021240475093 27.373315926223935 22.970752489041637 76-77 23.024178860767446 26.435294856496895 27.612309238208883 22.928217044526786 78-79 22.880311499089366 26.644652389625072 27.49670288262262 22.97833322866294 80-81 22.76719211203919 27.037844627268733 27.416290899956035 22.778672360736042 82-83 22.897029923338355 26.949242339173708 27.308647967123694 22.845079770364247 84-85 22.798539866903912 26.772807922560997 27.28424639381742 23.144405816717676 86-87 22.925202537210325 26.75922878854487 27.32954845192489 22.986020222319915 88-89 22.831401117879796 26.820021352760158 27.175180556427808 23.173396972932238 90-91 22.713209552270303 26.668676507349108 27.48954568807524 23.12856825230535 92-93 22.96079915028483 26.39814622215389 27.50756915742943 23.13348547013185 94-95 22.696878728882748 26.335137850907493 27.681994599007727 23.285988821202036 96-97 22.81532374552534 26.25819255165484 27.57349745650945 23.35298624631037 98-99 22.50028072691062 26.46286353506901 27.3136607618137 23.723194976206667 100-101 22.33831909494018 26.44976974780922 27.878843304647177 23.333067852603424 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 295.0 1 223.0 2 211.5 3 312.0 4 295.0 5 276.5 6 520.0 7 1232.0 8 2136.0 9 2658.5 10 2550.5 11 2341.5 12 2556.0 13 2841.5 14 3035.0 15 3241.0 16 3423.0 17 3593.0 18 3694.5 19 3767.0 20 3967.5 21 4343.0 22 4855.5 23 5592.5 24 6554.5 25 7821.0 26 9538.5 27 11557.5 28 12922.5 29 14896.5 30 18371.5 31 21728.5 32 25559.0 33 28885.0 34 31884.5 35 35905.0 36 40896.0 37 45709.5 38 49100.5 39 50804.0 40 52879.0 41 57041.0 42 61241.0 43 66521.0 44 70829.0 45 72103.0 46 72355.5 47 75781.5 48 81821.5 49 81669.0 50 78844.5 51 75854.0 52 71449.0 53 70698.0 54 70774.5 55 66373.5 56 60482.5 57 56384.5 58 52947.5 59 47906.5 60 40050.5 61 31766.5 62 25183.0 63 19374.5 64 14612.0 65 11420.5 66 8930.0 67 7252.5 68 5216.5 69 3251.5 70 2276.5 71 1687.5 72 1339.0 73 1172.5 74 937.0 75 721.5 76 518.0 77 305.0 78 173.5 79 107.0 80 70.0 81 44.5 82 25.0 83 16.5 84 8.5 85 2.5 86 2.5 87 3.0 88 1.5 89 1.0 90 0.5 91 0.0 92 0.5 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.03788230861018652 2 0.0 3 0.0 4 0.0 5 0.0155247126797714 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.004170068454436978 14-15 0.0 16-17 0.0 18-19 0.0 20-21 7.536268291151166E-5 22-23 2.5120894303837216E-4 24-25 4.2705520316523265E-4 26-27 0.0012560447151918608 28-29 0.002235759593041512 30-31 3.2657162594988383E-4 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.01072662186773849 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 5.024178860767443E-5 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 5.024178860767443E-5 74-75 2.5120894303837216E-5 76-77 0.0 78-79 0.0 80-81 0.0 82-83 2.7632983734220937E-4 84-85 4.2705520316523265E-4 86-87 0.0 88-89 0.0 90-91 0.008415499591785468 92-93 0.0030647491050681407 94-95 0.0 96-97 0.0 98-99 3.2657162594988383E-4 100-101 0.013791370972806631 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1990375.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 41.61947835768906 #Duplication Level Percentage of deduplicated Percentage of total 1 67.81311211936554 28.223463522194763 2 15.393497494504935 12.813386716433778 3 6.252296334535727 7.80651935943205 4 3.182377162866949 5.297955098237796 5 1.8483481392588195 3.846364268967864 6 1.1707894090814197 2.9236586683605426 7 0.7827232324928036 2.280357284435626 8 0.5569630039404199 1.8544407750825436 9 0.4036683145527982 1.5120418213093503 >10 2.2998261224886885 17.511520710761847 >50 0.19351247880254693 5.588076808513788 >100 0.09717111130474093 7.416049145048034 >500 0.004255908258862447 1.1624196255214256 >1k 0.0010943764094217723 0.5556273703077945 >5k 3.647921364739241E-4 1.208118825392811 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9947 0.49975507128053764 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8338 0.4189160334107894 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5587 0.28070087295107704 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2134 0.10721597688877725 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 5.024178860767443E-5 0.0 16-17 0.0 0.0 0.0 5.024178860767443E-5 0.0 18-19 0.0 0.0 0.0 5.024178860767443E-5 5.024178860767443E-5 20-21 0.0 0.0 0.0 7.536268291151164E-5 5.024178860767443E-5 22-23 0.0 0.0 0.0 2.2608804873453496E-4 5.024178860767443E-5 24-25 0.0 0.0 0.0 2.5120894303837216E-4 5.024178860767443E-5 26-27 0.0 0.0 0.0 3.2657162594988383E-4 5.024178860767443E-5 28-29 0.0 0.0 0.0 7.787477234189537E-4 5.024178860767443E-5 30-31 0.0 0.0 0.0 0.00198455065000314 5.024178860767443E-5 32-33 0.0 0.0 0.0 0.0036174087797525593 5.024178860767443E-5 34-35 0.0 0.0 0.0 0.00532562959241349 5.024178860767443E-5 36-37 0.0 0.0 0.0 0.007812598128493374 5.024178860767443E-5 38-39 0.0 0.0 0.0 0.012510205363310933 5.024178860767443E-5 40-41 0.0 0.0 0.0 0.01695660365509012 5.024178860767443E-5 42-43 0.0 0.0 0.0 0.021051309426615587 5.024178860767443E-5 44-45 0.0 0.0 0.0 0.024116058531683728 5.024178860767443E-5 46-47 0.0 0.0 0.0 0.029617534384224077 5.024178860767443E-5 48-49 0.0 0.0 0.0 0.03388808641587641 5.024178860767443E-5 50-51 0.0 0.0 0.0 0.038510330967782454 5.024178860767443E-5 52-53 0.0 0.0 0.0 0.05034227218488978 5.024178860767443E-5 54-55 0.0 0.0 0.0 0.0709414055140363 5.024178860767443E-5 56-57 0.0 0.0 0.0 0.10312127111725178 5.024178860767443E-5 58-59 0.0 0.0 0.0 0.13746153363059727 5.024178860767443E-5 60-61 0.0 0.0 0.0 0.18039314199585504 5.024178860767443E-5 62-63 0.0 0.0 0.0 0.20888023613640647 5.024178860767443E-5 64-65 0.0 0.0 0.0 0.24422533442190542 5.024178860767443E-5 66-67 0.0 0.0 0.0 0.31212711172517743 5.024178860767443E-5 68-69 0.0 0.0 0.0 0.40256233121899143 5.024178860767443E-5 70-71 0.0 0.0 0.0 0.49211831941217105 5.024178860767443E-5 72-73 0.0 0.0 0.0 0.6014444514224706 5.024178860767443E-5 74-75 0.0 0.0 0.0 0.7150662563587264 5.024178860767443E-5 76-77 0.0 0.0 0.0 0.821001067638008 5.024178860767443E-5 78-79 0.0 0.0 0.0 0.926383219242605 5.024178860767443E-5 80-81 0.0 0.0 0.0 1.0381963197889843 5.024178860767443E-5 82-83 0.0 0.0 0.0 1.1841235947999749 5.024178860767443E-5 84-85 0.0 0.0 0.0 1.3958173710984112 5.024178860767443E-5 86-87 0.0 0.0 0.0 1.6493374364127362 5.024178860767443E-5 88-89 0.0 0.0 0.0 1.9346354330214155 5.024178860767443E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 6160 0.0 45.675117 1 GTATCAA 10020 0.0 38.04042 1 ATCAACG 12675 0.0 29.834461 3 TCAACGC 12770 0.0 29.612514 4 TATCAAC 12900 0.0 29.424574 2 CAACGCA 13000 0.0 29.052057 5 AACGCAG 13385 0.0 28.246231 6 GTACATG 9825 0.0 25.976486 1 ACGCAGA 15055 0.0 25.02147 7 CGCAGAG 15215 0.0 24.695902 8 TACATGG 10335 0.0 24.36231 2 GTAAGGT 665 0.0 24.289042 4 TAAGGTG 725 0.0 24.244701 5 ACATGGG 10125 0.0 23.835363 3 GAGTACT 8405 0.0 23.79385 12-13 GCAGAGT 16365 0.0 22.728256 9 GTACTTT 9125 0.0 21.965717 14-15 CATGGGG 4450 0.0 21.56475 4 AGTACTT 8755 0.0 21.54045 12-13 AGAGTAC 14210 0.0 20.675976 10-11 >>END_MODULE