FastQCFastQC Report
Thu 26 May 2016
SRR937615_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937615_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2268465
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT75100.3310608715585208No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59710.26321763835897843No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37250.16420795559993212No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN29860.13163086051581135No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA104100.033.807941
GGTATCA70350.033.5316771
CGCGACT452.4160961E-531.66364178-79
ATCAACG130050.026.8312513
TATCAAC131000.026.637262
TCAACGC131050.026.6288644
GTACATG107850.026.4146211
TACATGG105750.026.3889792
CAACGCA133550.026.1659835
AACGCAG137300.025.4299586
ACATGGG107650.024.9069373
CATGGGG59350.022.7505174
CGCAGAG154250.022.2962918
ACGCAGA154950.022.288037
GAGTACT77300.021.84468712-13
AGTACTT78500.021.72253412-13
CGAATCG550.0028804421.58860820-21
GTACTTT78300.021.38158214-15
AGGACCG6950.021.2065735
GCAGAGT162200.020.7939
Produced by FastQC (version 0.11.5)