##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937615_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2268465 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.613157796130864 33.0 31.0 34.0 30.0 34.0 2 31.747177937503995 33.0 31.0 34.0 30.0 34.0 3 31.769901673598667 33.0 31.0 34.0 30.0 34.0 4 35.37680766509512 37.0 35.0 37.0 33.0 37.0 5 35.36566621041101 37.0 35.0 37.0 33.0 37.0 6 35.42773990341487 37.0 35.0 37.0 33.0 37.0 7 35.36467038283597 37.0 35.0 37.0 33.0 37.0 8 35.34064179963103 37.0 35.0 37.0 33.0 37.0 9 36.955721159462456 39.0 37.0 39.0 33.0 39.0 10-11 36.82007414705539 39.0 37.0 39.0 33.0 39.0 12-13 36.69034986213144 39.0 37.0 39.0 32.0 39.0 14-15 37.811168785941156 40.0 38.0 41.0 33.0 41.0 16-17 37.704658436431686 40.0 37.0 41.0 32.0 41.0 18-19 37.62718997207362 40.0 37.0 41.0 32.0 41.0 20-21 37.50835410729282 40.0 37.0 41.0 32.0 41.0 22-23 37.42050792055421 40.0 37.0 41.0 32.0 41.0 24-25 37.45205546481873 40.0 37.0 41.0 32.0 41.0 26-27 37.356237147145755 40.0 37.0 41.0 32.0 41.0 28-29 37.225894602737974 40.0 37.0 41.0 31.0 41.0 30-31 36.98530812686111 39.0 36.5 41.0 31.0 41.0 32-33 36.83407326099367 39.0 36.0 41.0 30.5 41.0 34-35 36.82621376128792 39.0 36.0 41.0 30.0 41.0 36-37 36.68039312927464 39.0 36.0 41.0 30.0 41.0 38-39 36.510495202703154 39.0 35.5 41.0 30.0 41.0 40-41 36.266641319129896 39.0 35.0 41.0 30.0 41.0 42-43 35.97652862177728 39.0 35.0 40.0 29.0 41.0 44-45 35.728852990899135 39.0 35.0 40.0 27.5 41.0 46-47 35.368098031047424 38.5 35.0 40.0 26.5 41.0 48-49 35.02085925945518 38.0 34.5 40.0 25.5 41.0 50-51 34.11548558165984 37.5 33.5 39.5 23.5 40.5 52-53 34.20136744450542 38.0 33.5 39.5 24.0 40.5 54-55 34.46307348801943 38.0 34.0 40.0 24.0 41.0 56-57 34.18701831414635 38.0 34.0 40.0 23.0 41.0 58-59 33.89552671079342 38.0 33.0 40.0 22.5 41.0 60-61 33.53008267705255 37.0 33.0 40.0 21.5 41.0 62-63 33.121512344250405 37.0 32.5 39.5 19.5 41.0 64-65 32.69074572453179 36.0 32.0 39.0 18.0 41.0 66-67 32.2107861483426 35.5 31.0 39.0 15.5 40.0 68-69 31.657565578485894 35.0 31.0 38.5 10.5 40.0 70-71 31.164435863017502 35.0 30.5 37.5 8.0 40.0 72-73 30.67995075965466 35.0 30.0 37.0 7.0 39.0 74-75 30.115127189531247 34.0 29.0 36.5 2.0 39.0 76-77 29.60506950735409 34.0 29.0 36.0 2.0 38.5 78-79 29.019403208777742 34.0 28.5 35.5 2.0 37.5 80-81 28.438641327946428 34.0 27.5 35.0 2.0 37.0 82-83 27.90683391632668 33.0 26.0 35.0 2.0 36.5 84-85 27.37327796549649 33.0 26.0 35.0 2.0 36.0 86-87 26.812781550519844 32.5 25.0 35.0 2.0 35.5 88-89 26.28770335887924 32.0 24.5 35.0 2.0 35.0 90-91 25.675946069258288 32.0 22.0 34.0 2.0 35.0 92-93 24.9871516642311 31.5 19.0 34.0 2.0 35.0 94-95 24.19038733240319 31.0 12.0 34.0 2.0 35.0 96-97 23.274631964786764 31.0 2.0 34.0 2.0 35.0 98-99 22.137129512688094 30.0 2.0 34.0 2.0 35.0 100-101 19.29844895116301 26.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.13401540648931132 1101 2 0.29684359865999355 1101 3 0.2501167957025743 1101 4 0.09751071425501578 1101 5 0.0767565932696499 1101 6 0.21914190747053652 1101 7 0.278181631559157 1101 8 0.28620209076531467 1101 9 0.25227046596639724 1101 10-11 0.27723219883285566 1101 12-13 0.3818717722464129 1101 14-15 0.44934382190123046 1101 16-17 0.5264143994870736 1101 18-19 0.4200082023450804 1101 20-21 0.40068634883638055 1101 22-23 0.3003463642226549 1101 24-25 0.3676002265935878 1101 26-27 0.3052576622994607 1101 28-29 0.31318559687925074 1101 30-31 0.41396917739380257 1101 32-33 0.3671841761386645 1101 34-35 0.35387294336001673 1101 36-37 0.45352070889070006 1101 38-39 0.3059878505940148 1101 40-41 0.3264219756232478 1101 42-43 0.4680438327948977 1101 44-45 0.5404186088440639 1101 46-47 0.6572489981270166 1101 48-49 0.6787354952596232 1101 50-51 0.5271389190850115 1101 52-53 0.5620072967492931 1101 54-55 0.724607051465803 1101 56-57 0.6155291092884383 1101 58-59 0.6177129766763017 1101 60-61 0.8616785536458735 1101 62-63 0.8675068977688554 1101 64-65 0.738363761457741 1101 66-67 0.6600894244983806 1101 68-69 0.7096279440801361 1101 70-71 0.8249327397290855 1101 72-73 0.6823513493609319 1101 74-75 0.8059472804227461 1101 76-77 0.8600284594428587 1101 78-79 0.8134566580502458 1101 80-81 0.9257985466266128 1101 82-83 0.7393352719329549 1101 84-85 0.8693174978886304 1101 86-87 0.8614775491586677 1101 88-89 0.8234629837542577 1101 90-91 1.003867647732502 1101 92-93 1.0490362465933991 1101 94-95 0.8966352586776303 1101 96-97 1.1665246414448767 1101 98-99 1.2106890161231298 1101 100-101 1.4262327118326468 1102 1 0.14121117219490742 1102 2 0.3114314751557181 1102 3 0.3152786194587236 1102 4 0.18566663961169638 1102 5 0.09940668862576274 1102 6 0.21914975322698638 1102 7 0.23481853799750496 1102 8 0.2521822872713386 1102 9 0.3965454778356161 1102 10-11 0.32118442823473004 1102 12-13 0.42684555937293567 1102 14-15 0.39842415842382906 1102 16-17 0.5162412697947047 1102 18-19 0.46905785994821514 1102 20-21 0.49770626901084114 1102 22-23 0.37261513569789884 1102 24-25 0.49519797667204557 1102 26-27 0.44519316615796356 1102 28-29 0.5328011950477745 1102 30-31 0.6288872797668503 1102 32-33 0.6253480880730606 1102 34-35 0.6534498760313525 1102 36-37 0.6963019088488593 1102 38-39 0.7080930279885749 1102 40-41 0.6798184840604549 1102 42-43 0.5821504023083008 1102 44-45 0.5805198794542648 1102 46-47 0.5602574433797329 1102 48-49 0.6295766005858852 1102 50-51 0.5865486969092899 1102 52-53 0.5288185399194631 1102 54-55 0.45921568904013554 1102 56-57 0.49636977203356025 1102 58-59 0.4835132237170541 1102 60-61 0.5752352161013121 1102 62-63 0.7311883882211703 1102 64-65 0.6871349952934764 1102 66-67 0.6620318527398865 1102 68-69 0.46921173675903205 1102 70-71 0.4391101222237914 1102 72-73 0.5429131457575167 1102 74-75 0.6183480014276448 1102 76-77 0.7576016060337771 1102 78-79 0.7301621846815642 1102 80-81 0.7543169660486804 1102 82-83 0.8112869943782535 1102 84-85 0.7473104528016847 1102 86-87 0.8343071916335809 1102 88-89 1.0788456756131772 1102 90-91 1.0680864719151337 1102 92-93 1.116474949106454 1102 94-95 1.2292554999245837 1102 96-97 1.5315792398628467 1102 98-99 1.4799003566611475 1102 100-101 1.690502432845598 1103 1 0.3077853466878331 1103 2 0.3925596104673481 1103 3 0.3346260208324523 1103 4 0.2279544075697899 1103 5 0.28954483078296533 1103 6 0.2731661441477584 1103 7 0.3815866960764396 1103 8 0.2898758285870713 1103 9 0.36776022120348273 1103 10-11 0.4771847858967746 1103 12-13 0.4471262471608952 1103 14-15 0.48801340471837307 1103 16-17 0.5675540433292525 1103 18-19 0.450853631075276 1103 20-21 0.4492810396071647 1103 22-23 0.444841620379961 1103 24-25 0.5105436012730422 1103 26-27 0.5374036329646188 1103 28-29 0.47465111543912997 1103 30-31 0.5344372909197475 1103 32-33 0.6149402187389299 1103 34-35 0.6751909210198548 1103 36-37 0.5811400187010989 1103 38-39 0.5616164798503434 1103 40-41 0.7042418982331 1103 42-43 0.6677501784810858 1103 44-45 0.5706420614234773 1103 46-47 0.6658337984573777 1103 48-49 0.7820538909690029 1103 50-51 0.6762398576572295 1103 52-53 0.8635571389610774 1103 54-55 0.9531012064194329 1103 56-57 0.8503740485947802 1103 58-59 0.8288254472981293 1103 60-61 0.9689872832340924 1103 62-63 1.0461624096501296 1103 64-65 1.17044294615917 1103 66-67 1.1683902570287614 1103 68-69 1.2095030390334607 1103 70-71 1.0920918891512095 1103 72-73 0.8336210473966084 1103 74-75 0.9679603419219127 1103 76-77 1.089185375868638 1103 78-79 1.1011303143013613 1103 80-81 1.1380117111087138 1103 82-83 1.3096404990817057 1103 84-85 1.357931765613678 1103 86-87 1.2882429163030302 1103 88-89 1.3462268010564067 1103 90-91 1.1892547769047148 1103 92-93 1.0440497312402748 1103 94-95 1.079981592332146 1103 96-97 1.301297186805801 1103 98-99 1.384609862010695 1103 100-101 1.1975463560520936 1104 1 0.2521248240349898 1104 2 0.3605594012434814 1104 3 0.2514556593304178 1104 4 0.3066632638417133 1104 5 0.18498849677256146 1104 6 0.20912698053953704 1104 7 0.21029317920727664 1104 8 0.25133853588579314 1104 9 0.22933325534668825 1104 10-11 0.1605352155321711 1104 12-13 0.2090563179579874 1104 14-15 0.3983340499802779 1104 16-17 0.4620338265888293 1104 18-19 0.31100248998186686 1104 20-21 0.52927623520727 1104 22-23 0.46421700964774715 1104 24-25 0.3832272945004789 1104 26-27 0.2892612212527297 1104 28-29 0.4060114483375443 1104 30-31 0.4253911677120783 1104 32-33 0.4784351097052095 1104 34-35 0.5156084194966866 1104 36-37 0.42849474130027687 1104 38-39 0.6079135564311215 1104 40-41 0.557650067843916 1104 42-43 0.5630775121263198 1104 44-45 0.5347889365778613 1104 46-47 0.5033028870840255 1104 48-49 0.3978484525045545 1104 50-51 0.40954064505574905 1104 52-53 0.6566987612403281 1104 54-55 0.6370180903011544 1104 56-57 0.7587292487280592 1104 58-59 0.6992239509600324 1104 60-61 0.6800339159406263 1104 62-63 0.8604057118226649 1104 64-65 0.9154860356207806 1104 66-67 0.9253169715263283 1104 68-69 0.9366694020349264 1104 70-71 0.8337662806342472 1104 72-73 0.7383116333881041 1104 74-75 0.737059139175269 1104 76-77 0.6960951877899433 1104 78-79 0.7788766439238763 1104 80-81 0.9827514259637056 1104 82-83 0.8790742482474698 1104 84-85 0.8733415877055855 1104 86-87 0.9549004778569845 1104 88-89 1.0972183381447316 1104 90-91 1.0569339514778129 1104 92-93 1.034035947649432 1104 94-95 1.1688329629906349 1104 96-97 1.1633856284463846 1104 98-99 1.4069246528788497 1104 100-101 1.101594256694007 1105 1 0.211156240414482 1105 2 0.25616200900508446 1105 3 0.1967856390860696 1105 4 0.22079517197697385 1105 5 0.2301922530904008 1105 6 0.18278186325684231 1105 7 0.21212084084943683 1105 8 0.217719321316018 1105 9 0.2223592572958495 1105 10-11 0.3099291197837246 1105 12-13 0.3485123737689406 1105 14-15 0.2519770946816351 1105 16-17 0.342660020490861 1105 18-19 0.32763735218811263 1105 20-21 0.3565429475638311 1105 22-23 0.5564102016427057 1105 24-25 0.3871361907734254 1105 26-27 0.4123868604947276 1105 28-29 0.38919769221529066 1105 30-31 0.3818583062530152 1105 32-33 0.34939658695999753 1105 34-35 0.4564122152482426 1105 36-37 0.3770381931606366 1105 38-39 0.5141640565141969 1105 40-41 0.6196803231416297 1105 42-43 0.523193553131847 1105 44-45 0.4674133982415114 1105 46-47 0.4802336720302236 1105 48-49 0.6509863071091431 1105 50-51 0.4108037684704229 1105 52-53 0.2725727266733955 1105 54-55 0.358952835411408 1105 56-57 0.4524121894397908 1105 58-59 0.2914523092907757 1105 60-61 0.5334076813404636 1105 62-63 0.4490677493087887 1105 64-65 0.5566929995825944 1105 66-67 0.5214621286773706 1105 68-69 0.5965444548585133 1105 70-71 0.40443056283895373 1105 72-73 0.55048754251197 1105 74-75 0.38009287574266537 1105 76-77 0.29223135579335846 1105 78-79 0.44602994287190967 1105 80-81 0.5295946645145442 1105 82-83 0.33091345748697165 1105 84-85 0.3158903646974771 1105 86-87 0.4137734481617805 1105 88-89 0.5444506586151547 1105 90-91 0.4226632509031596 1105 92-93 0.23585979631248577 1105 94-95 0.42173048137343727 1105 96-97 0.5831592639283478 1105 98-99 0.8375063060482937 1105 100-101 1.0502733360235297 1106 1 0.06556720258825521 1106 2 0.15360644681937785 1106 3 0.1191905039304082 1106 4 0.15567482566601853 1106 5 0.14930173921138135 1106 6 0.09291135013727825 1106 7 0.26488465612856515 1106 8 0.22953273435584265 1106 9 0.2731736630503434 1106 10-11 -0.007998330675469845 1106 12-13 0.0206085206256148 1106 14-15 0.08894481246858277 1106 16-17 0.06587793492211347 1106 18-19 0.22197041609615553 1106 20-21 0.134970552626136 1106 22-23 0.26581451859533445 1106 24-25 0.1439423618783806 1106 26-27 0.27589401834720917 1106 28-29 0.37043983322416807 1106 30-31 0.36937917767205874 1106 32-33 0.27397902296245036 1106 34-35 0.5031709204879249 1106 36-37 0.3775920519998621 1106 38-39 0.3724220863824357 1106 40-41 0.4417540661099011 1106 42-43 0.4300672613822272 1106 44-45 0.46660631579194956 1106 46-47 0.43717665332053457 1106 48-49 0.49998149542657444 1106 50-51 0.3387330090708289 1106 52-53 0.3253039939306248 1106 54-55 0.3605418298402725 1106 56-57 0.2869356592759189 1106 58-59 0.3415188699955465 1106 60-61 0.2115586282166717 1106 62-63 0.4949578563375425 1106 64-65 0.45092115036673164 1106 66-67 0.26750087167276604 1106 68-69 0.04746149537538358 1106 70-71 0.02319590787510961 1106 72-73 0.39306784119431626 1106 74-75 0.4477720390415172 1106 76-77 0.5709747851614395 1106 78-79 0.49703605647535554 1106 80-81 0.28989660166431364 1106 82-83 0.2451824017918831 1106 84-85 0.4287198184874228 1106 86-87 0.5597613077496142 1106 88-89 0.5594851323950074 1106 90-91 0.5558379307119949 1106 92-93 0.7345856497487482 1106 94-95 0.8911164629404098 1106 96-97 1.0588215890505417 1106 98-99 1.0391528019825031 1106 100-101 1.1328722955717652 1107 1 0.2793012613094312 1107 2 0.244299383098312 1107 3 0.2187662841995639 1107 4 0.33280991079629985 1107 5 0.3265929883028136 1107 6 0.2497195702205346 1107 7 0.2563940942466729 1107 8 0.19780347729102488 1107 9 0.2519525445727737 1107 10-11 0.29381825926747496 1107 12-13 0.3307357204670254 1107 14-15 0.42612394949199484 1107 16-17 0.5638489047245443 1107 18-19 0.5892056091535736 1107 20-21 0.547910025324974 1107 22-23 0.393374153845663 1107 24-25 0.5192713193903202 1107 26-27 0.44980813918154894 1107 28-29 0.4429390072511765 1107 30-31 0.5711551297435946 1107 32-33 0.627176639755028 1107 34-35 0.5335258686168274 1107 36-37 0.5083942369737571 1107 38-39 0.626945224639293 1107 40-41 0.6113284146925295 1107 42-43 0.6287965845248848 1107 44-45 0.6612046726563321 1107 46-47 0.6481206853903174 1107 48-49 0.640405384768215 1107 50-51 0.5333885232665452 1107 52-53 0.5267730144367846 1107 54-55 0.5871020377652059 1107 56-57 0.6504068383248338 1107 58-59 0.6952513869004591 1107 60-61 0.7642962331152461 1107 62-63 1.0010834923367469 1107 64-65 1.1129737483271285 1107 66-67 1.0041087657720098 1107 68-69 0.9931333968753471 1107 70-71 1.048665170666677 1107 72-73 1.0626685107830944 1107 74-75 0.9376729222819726 1107 76-77 0.9574161631046536 1107 78-79 1.1767878240575698 1107 80-81 1.3111652961864628 1107 82-83 1.0749205132872213 1107 84-85 1.1991546106726148 1107 86-87 1.2922560765873037 1107 88-89 1.2474820928143835 1107 90-91 1.5033888657134291 1107 92-93 1.46053408700784 1107 94-95 1.6498136282010876 1107 96-97 1.728812007763782 1107 98-99 1.784539137085254 1107 100-101 1.4539273384122282 1108 1 -0.06182586695768677 1108 2 0.07539414405641409 1108 3 0.06532542613287973 1108 4 0.1585084552663858 1108 5 0.125954799540132 1108 6 0.17708959316403394 1108 7 0.18311144305770455 1108 8 0.1603229992405204 1108 9 0.3746161543269224 1108 10-11 0.3036537420964862 1108 12-13 0.22884737769322783 1108 14-15 0.22848856123413697 1108 16-17 0.3103220501809645 1108 18-19 0.3495405874921218 1108 20-21 0.3356548197492728 1108 22-23 0.41084151181411244 1108 24-25 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-1.3848898026356693 2316 98-99 -1.3917104478473732 2316 100-101 -1.3345331572023582 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 9345.0 3 2030.0 4 2077.0 5 2212.0 6 2423.0 7 2967.0 8 3667.0 9 4657.0 10 5980.0 11 7303.0 12 9498.0 13 11575.0 14 13545.0 15 16825.0 16 20319.0 17 25104.0 18 22377.0 19 17782.0 20 17892.0 21 19241.0 22 20964.0 23 23381.0 24 26559.0 25 29781.0 26 34372.0 27 40118.0 28 47648.0 29 56253.0 30 68433.0 31 83637.0 32 104248.0 33 133249.0 34 176261.0 35 238505.0 36 327281.0 37 391444.0 38 232043.0 39 17466.0 40 3.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.37498313956548 18.00398105263065 14.488563871306129 23.132471936497744 2 15.409823890379421 19.104469958095663 40.928455825825786 24.55725032569913 3 19.80993447571287 23.418014183519283 28.82224271483275 27.949808625935095 4 12.764498571186056 15.728624377546401 36.03338791591218 35.473489135355365 5 13.868940694508122 37.421761395966364 33.60622433771577 15.103073571809741 6 33.40795132103874 34.94736693321837 16.925753629897137 14.71892811584575 7 30.03939527654212 32.30399425435859 19.312254497285085 18.344355971814203 8 27.182799536559955 34.818340953532754 18.726122921156442 19.272736588750845 9 26.758100464398062 17.09416847835209 19.54875561283848 36.598975444411366 10-11 25.486052903882705 26.462675408518116 26.205411422494475 21.845860265104708 12-13 26.38102412332571 23.519894737469762 27.057421567114027 23.041659572090502 14-15 23.26531180130369 24.985645117263932 25.47048680278334 26.278556278649035 16-17 22.548653240148102 28.42328517702809 26.108164839271375 22.919896743552435 18-19 22.56390595700315 26.910233190572907 28.427713263336337 22.098147589087606 20-21 23.19829538252533 25.8029425806785 28.785780250269077 22.21298178652709 22-23 22.998944229666375 25.55706183598925 28.781301845864597 22.66269208847978 24-25 22.684313609011415 25.857070897009717 28.848678566199553 22.609936927779316 26-27 22.27966093835086 26.284763734023954 28.777157459272367 22.658417868352817 28-29 22.289212239215242 26.661553153446032 28.622518481087805 22.426716126250916 30-31 22.48022499793493 26.18326447549587 28.55217875091161 22.784331775657588 32-33 22.221660233118477 26.175857286769126 28.652042630382883 22.950439849729513 34-35 22.832877169795317 26.235572981454258 28.47114242903992 22.460407419710503 36-37 22.895801628190647 25.926688141518504 28.473264339409827 22.70424589088102 38-39 22.415190188676245 26.290677202308434 28.479086730487552 22.81504587852777 40-41 22.85759835153928 26.24558081323856 28.330754669361696 22.566066165860462 42-43 22.444265471103154 26.11736818499317 28.28798552745105 23.150380816452632 44-45 22.55405850971146 26.367090147905202 28.34352487123195 22.735326471151385 46-47 22.936569195484545 26.733870656061555 27.687513595943468 22.642046552510433 48-49 22.895684092734577 26.66287991134399 27.597297122411945 22.84413887350949 50-51 23.01221566640665 26.943548038010974 27.52228599394517 22.521950301637204 52-53 23.01526921596877 26.78432812312923 27.406102119646924 22.79430054125507 54-55 22.74960908611721 26.89670797623314 27.449408644071504 22.904274293578148 56-57 22.90542745472779 27.18635560958571 27.274539770968463 22.633677164718033 58-59 23.065419974728503 26.659002347648475 27.338678927222016 22.936898750401006 60-61 22.70087637806123 26.895529624121767 27.665009696323168 22.738584301493834 62-63 22.628856339612902 26.93524345608751 27.851202426601297 22.584697777698292 64-65 22.566700902827883 26.882201405772694 27.702036771933297 22.849060919466126 66-67 22.565307239874947 26.98953052969956 27.41166522422594 23.033497006199553 68-69 22.650650720494568 26.808986635394927 27.701167368786656 22.83919527532385 70-71 22.7058843270516 26.807629926402303 27.543783496400483 22.94270225014561 72-73 22.680810684668142 26.87480433416656 27.43035179447371 23.014033186691588 74-75 22.584491224491014 27.45175186718712 27.119724080007256 22.84403282831461 76-77 22.92072530268234 26.956497162791244 27.21538873590918 22.90738879861724 78-79 22.855120866017852 27.03285104691666 27.20843936826184 22.90358871880365 80-81 22.90176382259372 27.202791134940696 27.0226046692162 22.872840373249385 82-83 22.891180027792167 27.211506150793912 27.08912612759403 22.808187693819885 84-85 22.84175270602588 27.311428150705147 27.05105270582719 22.795766437441785 86-87 22.85683693935243 27.263645786305702 27.030362971458345 22.84915430288353 88-89 22.855522973551174 27.19218726611275 26.860357140176262 23.091932620159817 90-91 22.92724188805541 27.031977040720573 27.047719814831417 22.993061256392604 92-93 22.87103081212209 26.82182820416133 27.23834069688682 23.068800286829763 94-95 22.781782839964425 26.989173526494785 27.129593567878963 23.099450065661824 96-97 22.886331813306498 26.799160842742797 27.051318678843955 23.263188665106753 98-99 22.65159336529808 26.957870296364266 26.947802195375914 23.442734142961736 100-101 23.21077677185116 26.10963780902738 27.481258258352387 23.198327160769068 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 2640.0 1 1482.0 2 316.0 3 292.0 4 261.5 5 270.0 6 403.5 7 745.0 8 1267.5 9 1592.0 10 1604.5 11 1596.5 12 1716.5 13 1956.5 14 2186.0 15 2372.5 16 2533.5 17 2720.0 18 2939.0 19 3141.5 20 3441.5 21 3983.5 22 4599.0 23 5351.0 24 6524.0 25 8341.0 26 10969.5 27 13943.0 28 16257.5 29 18661.5 30 22225.5 31 26662.0 32 32348.0 33 36304.0 34 39265.5 35 43314.5 36 48462.0 37 54317.5 38 57629.5 39 60104.0 40 63093.0 41 67237.5 42 70028.0 43 72177.5 44 76180.0 45 80518.0 46 84367.5 47 88763.0 48 92338.5 49 91425.0 50 88610.0 51 84954.5 52 82453.0 53 83143.0 54 85282.5 55 80204.0 56 70112.0 57 63299.5 58 58658.0 59 52325.5 60 42992.0 61 35125.0 62 28898.0 63 22192.5 64 16149.0 65 11986.5 66 8679.0 67 6324.5 68 4670.5 69 3493.5 70 2659.5 71 1830.0 72 1270.5 73 1009.0 74 756.0 75 542.5 76 404.0 77 269.0 78 166.0 79 125.5 80 92.0 81 58.0 82 47.5 83 39.0 84 27.0 85 18.0 86 10.5 87 7.0 88 6.5 89 7.5 90 6.5 91 3.0 92 2.0 93 2.0 94 2.0 95 2.0 96 1.5 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.31977570736158595 2 0.28248176630452754 3 0.28226135294130617 4 0.28428915588294285 5 0.2924444503221341 6 0.1978871175001598 7 0.1978430348275155 8 0.19951817638799804 9 0.19656463732083146 10-11 0.20465380775105632 12-13 0.19447071037022834 14-15 0.19515399179621462 16-17 0.20220721941929895 18-19 0.19464704106080544 20-21 0.18788035080990892 22-23 0.1869766560207012 24-25 0.19019469112373347 26-27 0.18585254786827216 28-29 0.1996504244059309 30-31 0.20375011296184867 32-33 0.20632894931153886 34-35 0.20529300650439833 36-37 0.18122386724062306 38-39 0.17183425796739205 40-41 0.17851278287300001 42-43 0.190282856469022 44-45 0.1945588757155169 46-47 0.19671892667508648 48-49 0.19544052916840243 50-51 0.19779895215487125 52-53 0.22138318201955948 54-55 0.18600683722252714 56-57 0.17130526589566072 58-59 0.1700929923979431 60-61 0.16262097938473816 62-63 0.17185629930371418 64-65 0.16764640406618572 66-67 0.16685291595858875 68-69 0.17692580665780605 70-71 0.1699827857163324 72-73 0.16583901448777036 74-75 0.17172405128578136 76-77 0.17648497993136328 78-79 0.17974709770703978 80-81 0.17044565377909732 82-83 0.16742599070296435 84-85 0.1612764578690877 86-87 0.15946906829067234 88-89 0.1653981877613276 90-91 0.17342123418258604 92-93 0.16341446749233512 94-95 0.1688807189002255 96-97 0.16804314811998422 98-99 0.171349348568305 100-101 0.14697163059601978 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2268465.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 49.584366187221605 #Duplication Level Percentage of deduplicated Percentage of total 1 74.33128432072084 36.856696209251055 2 13.596956959079897 13.483929857818175 3 4.8879881320584895 7.2710338037634425 4 2.2307807902615475 4.424474063509926 5 1.26290100092625 3.1310072844067935 6 0.774598469902176 2.3044784507814624 7 0.5193215734080411 1.8025161745351872 8 0.36676470365745506 1.4548636296559252 9 0.25691634080676073 1.1465130529839103 >10 1.5701702006162923 14.544950326573328 >50 0.1301545376305229 4.425709287392109 >100 0.06630120787024352 5.96432063027492 >500 0.004351915000196042 1.4788892354414445 >1k 0.0013322188776109924 1.1169396604877762 >5k 1.7762918368146566E-4 0.5936783331244613 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7510 0.3310608715585208 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5971 0.26321763835897843 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3725 0.16420795559993212 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 2986 0.13163086051581135 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 4.408267264427708E-5 10-11 0.0 0.0 0.0 0.0 4.408267264427708E-5 12-13 0.0 0.0 0.0 0.0 6.612400896641562E-5 14-15 0.0 0.0 0.0 0.0 8.816534528855416E-5 16-17 0.0 0.0 0.0 2.204133632213854E-5 8.816534528855416E-5 18-19 2.204133632213854E-5 0.0 0.0 4.408267264427708E-5 8.816534528855416E-5 20-21 4.408267264427708E-5 0.0 0.0 1.102066816106927E-4 8.816534528855416E-5 22-23 4.408267264427708E-5 0.0 0.0 1.5428935425496976E-4 1.7633069057710832E-4 24-25 4.408267264427708E-5 0.0 0.0 2.2041336322138537E-4 3.085787085099395E-4 26-27 4.408267264427708E-5 0.0 0.0 4.187853901206323E-4 3.5266138115421663E-4 28-29 4.408267264427708E-5 0.0 0.0 9.257361255298187E-4 3.5266138115421663E-4 30-31 4.408267264427708E-5 0.0 0.0 0.0021820922958917153 3.7470271747635516E-4 32-33 4.408267264427708E-5 0.0 0.0 0.004716845972937648 3.967440537984937E-4 34-35 4.408267264427708E-5 0.0 0.0 0.006876896932507225 4.4082672644277075E-4 36-37 4.408267264427708E-5 0.0 0.0 0.008706327847244722 4.4082672644277075E-4 38-39 4.408267264427708E-5 0.0 0.0 0.010579841434626498 4.4082672644277075E-4 40-41 4.408267264427708E-5 0.0 0.0 0.012673768385229659 4.4082672644277075E-4 42-43 4.408267264427708E-5 0.0 0.0 0.015473018098141254 4.4082672644277075E-4 44-45 4.408267264427708E-5 0.0 0.0 0.018669011864851343 4.4082672644277075E-4 46-47 4.408267264427708E-5 0.0 0.0 0.022482163048581312 4.4082672644277075E-4 48-49 4.408267264427708E-5 0.0 0.0 0.028587613209813685 4.4082672644277075E-4 50-51 4.408267264427708E-5 0.0 0.0 0.03647841161313928 4.4082672644277075E-4 52-53 4.408267264427708E-5 0.0 0.0 0.05217184307450192 4.4082672644277075E-4 54-55 4.408267264427708E-5 0.0 0.0 0.072229459127648 4.4082672644277075E-4 56-57 6.612400896641562E-5 0.0 0.0 0.09629859839142327 5.069507354091864E-4 58-59 8.816534528855416E-5 0.0 0.0 0.12446742621111632 5.510334080534634E-4 60-61 8.816534528855416E-5 0.0 0.0 0.16619167586892458 5.73074744375602E-4 62-63 8.816534528855416E-5 0.0 0.0 0.20037778850456145 5.73074744375602E-4 64-65 8.816534528855416E-5 0.0 0.0 0.23224956082637377 6.17157417019879E-4 66-67 8.816534528855416E-5 0.0 0.0 0.28598633877974755 6.612400896641562E-4 68-69 8.816534528855416E-5 2.204133632213854E-5 0.0 0.3574443511361207 6.832814259862947E-4 70-71 8.816534528855416E-5 4.408267264427708E-5 0.0 0.4240753108379455 7.053227623084333E-4 72-73 8.816534528855416E-5 4.408267264427708E-5 0.0 0.4935496029253261 7.053227623084333E-4 74-75 8.816534528855416E-5 4.408267264427708E-5 0.0 0.5946311712986535 7.053227623084333E-4 76-77 8.816534528855416E-5 4.408267264427708E-5 0.0 0.6855957663001193 7.053227623084333E-4 78-79 8.816534528855416E-5 4.408267264427708E-5 0.0 0.7771775187186049 7.273640986305718E-4 80-81 8.816534528855416E-5 4.408267264427708E-5 0.0 0.8835710491455676 7.714467712748489E-4 82-83 8.816534528855416E-5 4.408267264427708E-5 0.0 1.0298153156429568 7.934881075969874E-4 84-85 8.816534528855416E-5 4.408267264427708E-5 0.0 1.1830687270907863 7.934881075969874E-4 86-87 8.816534528855416E-5 4.408267264427708E-5 0.0 1.3567103746365934 8.375707802412644E-4 88-89 8.816534528855416E-5 4.408267264427708E-5 0.0 1.5766829111315361 8.375707802412644E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 10410 0.0 33.80794 1 GGTATCA 7035 0.0 33.531677 1 CGCGACT 45 2.4160961E-5 31.663641 78-79 ATCAACG 13005 0.0 26.831251 3 TATCAAC 13100 0.0 26.63726 2 TCAACGC 13105 0.0 26.628864 4 GTACATG 10785 0.0 26.414621 1 TACATGG 10575 0.0 26.388979 2 CAACGCA 13355 0.0 26.165983 5 AACGCAG 13730 0.0 25.429958 6 ACATGGG 10765 0.0 24.906937 3 CATGGGG 5935 0.0 22.750517 4 CGCAGAG 15425 0.0 22.296291 8 ACGCAGA 15495 0.0 22.28803 7 GAGTACT 7730 0.0 21.844687 12-13 AGTACTT 7850 0.0 21.722534 12-13 CGAATCG 55 0.00288044 21.588608 20-21 GTACTTT 7830 0.0 21.381582 14-15 AGGACCG 695 0.0 21.206573 5 GCAGAGT 16220 0.0 20.793 9 >>END_MODULE