FastQCFastQC Report
Thu 26 May 2016
SRR937615_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937615_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2268465
Sequences flagged as poor quality0
Sequence length101
%GC45

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT91450.4031360413319139No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT78950.34803270052656754No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50120.22094235529311673No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA73550.037.545731
GTATCAA109550.034.7525941
ATCAACG139150.027.081983
TATCAAC141250.026.813922
TCAACGC140950.026.7361324
CAACGCA143400.026.312485
AACGCAG146100.025.8781416
GTACATG104850.024.7509161
TACATGG102750.024.7435722
ACATGGG102350.023.5402223
TAAGGTG11450.022.4119015
ACGCAGA168400.022.3963577
GTAAGGT10650.022.3105814
CGCAGAG169300.022.1370128
ATCGGAC3059.094947E-1221.8035458
GAGTACT94900.021.69810512-13
GTACTTT96700.021.0717714-15
AGTACTT96900.020.78456912-13
GCAGAGT179350.020.5522469
TAGCGCG1856.5310724E-620.5498524
Produced by FastQC (version 0.11.5)