##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937615_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2268465 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality warn #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.068790129007944 31.0 31.0 34.0 30.0 34.0 2 32.35481217475253 34.0 31.0 34.0 31.0 34.0 3 32.45339161062657 34.0 31.0 34.0 31.0 34.0 4 36.10871756892877 37.0 35.0 37.0 35.0 37.0 5 36.01658169731515 37.0 35.0 37.0 35.0 37.0 6 36.059609471603046 37.0 35.0 37.0 35.0 37.0 7 36.0016103400317 37.0 35.0 37.0 35.0 37.0 8 35.970298417652465 37.0 35.0 37.0 35.0 37.0 9 37.65862378304272 39.0 37.0 39.0 35.0 39.0 10-11 37.564994610893265 39.0 37.0 39.0 35.0 39.0 12-13 37.453308294375276 39.0 37.0 39.0 35.0 39.0 14-15 38.65966721990421 40.0 38.0 41.0 34.0 41.0 16-17 38.60758795044226 40.0 38.0 41.0 34.0 41.0 18-19 38.57232732266092 40.0 38.0 41.0 34.0 41.0 20-21 38.45769738567709 40.0 38.0 41.0 34.0 41.0 22-23 38.40690973852362 40.0 38.0 41.0 34.0 41.0 24-25 38.43620686234965 40.0 38.0 41.0 34.0 41.0 26-27 38.29406448854181 40.0 38.0 41.0 34.0 41.0 28-29 38.14036474003346 40.0 38.0 41.0 33.5 41.0 30-31 38.19438673287884 40.0 38.0 41.0 34.0 41.0 32-33 38.117100991198896 40.0 37.0 41.0 34.0 41.0 34-35 37.9713367850066 40.0 37.0 41.0 33.0 41.0 36-37 37.815622458358405 40.0 37.0 41.0 33.0 41.0 38-39 37.667888858765735 40.0 37.0 41.0 33.0 41.0 40-41 37.483318455431316 40.0 37.0 41.0 33.0 41.0 42-43 37.24798398917329 40.0 36.5 41.0 32.0 41.0 44-45 36.948487633708254 39.5 36.0 41.0 31.0 41.0 46-47 36.57929855651288 39.0 36.0 41.0 30.5 41.0 48-49 36.23763712466359 39.0 35.5 41.0 30.0 41.0 50-51 35.959747229955056 39.0 35.0 40.0 29.0 41.0 52-53 35.69916661707366 39.0 35.0 40.0 28.5 41.0 54-55 35.45811396693359 38.0 35.0 40.0 28.0 41.0 56-57 35.15157275955326 38.0 34.0 40.0 27.5 41.0 58-59 34.858812236468275 38.0 34.0 40.0 27.0 41.0 60-61 34.495357212917106 37.5 34.0 40.0 26.0 41.0 62-63 34.092851553804 37.0 33.5 39.5 26.0 41.0 64-65 33.647676732944966 36.0 33.0 39.0 25.0 40.5 66-67 33.187110667345536 36.0 32.0 39.0 23.5 40.0 68-69 32.711330789763124 35.0 32.0 38.5 22.5 40.0 70-71 32.28768131754292 35.0 31.5 38.0 22.0 40.0 72-73 31.77419885252803 35.0 31.0 37.0 20.5 39.0 74-75 31.281673510501594 34.5 31.0 36.5 19.5 39.0 76-77 30.106070184023118 33.5 29.5 35.0 18.5 37.5 78-79 30.69830061296956 34.0 30.0 36.0 18.5 37.5 80-81 30.518498852748444 34.0 30.0 35.0 18.0 37.0 82-83 30.13799882299264 34.0 30.0 35.0 16.0 36.5 84-85 29.745282823407017 34.0 30.0 35.0 9.5 36.0 86-87 29.340262688646288 34.0 29.0 35.0 7.0 36.0 88-89 28.856141928572846 34.0 29.0 35.0 2.0 35.0 90-91 28.263186560074764 33.0 28.0 35.0 2.0 35.0 92-93 27.76915050485681 33.0 27.0 35.0 2.0 35.0 94-95 27.23667502033313 33.0 26.0 34.5 2.0 35.0 96-97 26.581719356481145 32.0 25.0 34.0 2.0 35.0 98-99 25.56400054662514 32.0 23.5 34.0 2.0 35.0 100-101 22.641387458038807 28.5 8.5 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.28914466850132925 1101 2 0.20951520555929193 1101 3 0.19830896425924038 1101 4 0.10450016987423538 1101 5 0.02647400388846677 1101 6 0.07176382716966856 1101 7 0.13114865756035954 1101 8 0.12358527138970032 1101 9 0.1491789362246152 1101 10-11 0.23088214517955663 1101 12-13 0.2625246258698084 1101 14-15 0.25202116787405515 1101 16-17 0.2413152388116302 1101 18-19 0.3091784097522563 1101 20-21 0.21239666785893974 1101 22-23 0.3008291099670899 1101 24-25 0.21280218396827166 1101 26-27 0.08262523394348165 1101 28-29 -0.004613536426042231 1101 30-31 0.047684944824311515 1101 32-33 0.12021281456021882 1101 34-35 0.12853029701540208 1101 36-37 0.057310885532061207 1101 38-39 0.10134981875053484 1101 40-41 0.09327179245886441 1101 42-43 0.14178573210013212 1101 44-45 0.15261789600754838 1101 46-47 0.0813649184988563 1101 48-49 0.13860149209553896 1101 50-51 0.06204568978490954 1101 52-53 0.0744813331962817 1101 54-55 0.011641170538780443 1101 56-57 -0.17812461927280054 1101 58-59 -0.1752352485270663 1101 60-61 -0.04384414307372708 1101 62-63 -0.15812104339842392 1101 64-65 0.003648805447383552 1101 66-67 -0.11418421373927146 1101 68-69 -0.12531746135184818 1101 70-71 -0.20236170051627056 1101 72-73 -0.2318224928484156 1101 74-75 -0.1312838571984294 1101 76-77 -0.11115393413586006 1101 78-79 -0.14547059905769544 1101 80-81 -0.19675990285695022 1101 82-83 -0.026786222494408207 1101 84-85 -0.0016453220117078615 1101 86-87 0.014275090911642252 1101 88-89 0.02579592158668831 1101 90-91 -0.13447661632829266 1101 92-93 -0.3163692266495737 1101 94-95 -0.4033885876731418 1101 96-97 -0.48626671326797677 1101 98-99 -0.38646400554384996 1101 100-101 -0.22878160184922436 1102 1 0.3052809754434165 1102 2 0.2522377635173072 1102 3 0.19336774708065718 1102 4 0.1458573650364201 1102 5 0.11280810436242916 1102 6 0.15253588983915733 1102 7 0.14212889430103814 1102 8 0.2012655024975203 1102 9 0.20860631670758778 1102 10-11 0.19699089138745052 1102 12-13 0.27353966558140286 1102 14-15 0.3771923663032979 1102 16-17 0.3185644562477208 1102 18-19 0.31248157380500885 1102 20-21 0.4097603931045626 1102 22-23 0.3833975491041386 1102 24-25 0.24681544933187638 1102 26-27 0.2921226227147926 1102 28-29 0.2650789099210513 1102 30-31 0.2989960914313272 1102 32-33 0.2390989585519776 1102 34-35 0.1911989807791059 1102 36-37 0.2965304138210456 1102 38-39 0.36341910683866274 1102 40-41 0.27876760403196243 1102 42-43 0.274259820986515 1102 44-45 0.2516680565437994 1102 46-47 0.17258722700204743 1102 48-49 0.10498222452708461 1102 50-51 0.16601775486847004 1102 52-53 0.16902722483632004 1102 54-55 0.16167084359203443 1102 56-57 0.2859967364739191 1102 58-59 0.16899359408304093 1102 60-61 0.12458292357899126 1102 62-63 0.17144679624235692 1102 64-65 0.16328490316929845 1102 66-67 0.16635297916977265 1102 68-69 0.1502928982654339 1102 70-71 0.01722764454412129 1102 72-73 -0.00909082174252518 1102 74-75 -0.059265546409907444 1102 76-77 -0.11870306064164282 1102 78-79 -0.06265129688743443 1102 80-81 0.11926445117576989 1102 82-83 0.21233312151988315 1102 84-85 0.1895571709271131 1102 86-87 0.06655709958623035 1102 88-89 0.1453120395868659 1102 90-91 0.055374696528289746 1102 92-93 0.18956880394485154 1102 94-95 -0.1586395110583254 1102 96-97 -0.16980852913813038 1102 98-99 -0.06329991255352851 1102 100-101 -0.10271920595637596 1103 1 0.3265101406678994 1103 2 0.2478452505760913 1103 3 0.2862064299613323 1103 4 0.1989059532328028 1103 5 0.14913483825417728 1103 6 0.17004421605770403 1103 7 0.12294570980126451 1103 8 0.19305388963399395 1103 9 0.25366481175800715 1103 10-11 0.22430704155935643 1103 12-13 0.30438820916618425 1103 14-15 0.24961585354667903 1103 16-17 0.19121302059996736 1103 18-19 0.23605236781474304 1103 20-21 0.11775942780163007 1103 22-23 0.1747458773225219 1103 24-25 0.2390898530361767 1103 26-27 0.18569585882558215 1103 28-29 0.2582544873721915 1103 30-31 0.27226421874362927 1103 32-33 0.24550226288776855 1103 34-35 0.22515072332396358 1103 36-37 0.20284718701715576 1103 38-39 0.2717325380098714 1103 40-41 0.2284868585565718 1103 42-43 0.27911296802791696 1103 44-45 0.2948281545355087 1103 46-47 0.09478736775936625 1103 48-49 -0.08107895491158246 1103 50-51 -0.04243508758331416 1103 52-53 0.08713905768123453 1103 54-55 -0.019379125256755003 1103 56-57 0.10237438913407004 1103 58-59 0.003188296838963822 1103 60-61 0.02047634798154263 1103 62-63 0.012449288339183795 1103 64-65 0.05301586824148785 1103 66-67 0.1824601739456 1103 68-69 0.29013048893083493 1103 70-71 0.14793475778838427 1103 72-73 0.2654448906202731 1103 74-75 0.25617516629901615 1103 76-77 0.21389946914429814 1103 78-79 0.14088773057946824 1103 80-81 0.04333308767575872 1103 82-83 0.051079073785462725 1103 84-85 0.017115415998684824 1103 86-87 -0.08530380064528842 1103 88-89 -0.005028604666311054 1103 90-91 0.14367366738459708 1103 92-93 0.07336459814687757 1103 94-95 -0.02869944829440385 1103 96-97 -0.11073025883792553 1103 98-99 -0.07096099507731424 1103 100-101 0.15740186072259732 1104 1 0.2915147368125588 1104 2 0.2585770788536621 1104 3 0.13462315740947162 1104 4 0.08726681015951954 1104 5 0.04143782153467157 1104 6 0.09928896633061868 1104 7 0.06511933267717751 1104 8 0.09111266023067799 1104 9 0.11244044531402864 1104 10-11 0.18320879061275974 1104 12-13 0.19040788977044798 1104 14-15 0.15663250524803374 1104 16-17 0.2630269818373776 1104 18-19 0.19413727202218212 1104 20-21 0.21763457152195542 1104 22-23 0.22703818631130446 1104 24-25 0.1848545711118632 1104 26-27 0.19849125435070647 1104 28-29 0.21161354749208527 1104 30-31 0.3528565741297385 1104 32-33 0.1416037248395341 1104 34-35 0.2727969063929123 1104 36-37 0.15637595093998868 1104 38-39 0.024994057188244767 1104 40-41 0.018883386215293285 1104 42-43 0.08691634021296579 1104 44-45 0.09335554784828304 1104 46-47 0.05193890105066146 1104 48-49 0.10567175000817741 1104 50-51 0.10409930310544979 1104 52-53 0.15392999945016328 1104 54-55 0.1063504703309448 1104 56-57 0.29543241872956827 1104 58-59 0.1960097872296771 1104 60-61 0.06647702408459111 1104 62-63 0.011229396961240923 1104 64-65 0.07986674877368927 1104 66-67 -0.020539863830933314 1104 68-69 0.049144211498052925 1104 70-71 0.10050380408594606 1104 72-73 0.09439256237514826 1104 74-75 0.031168071091503435 1104 76-77 -0.08539246304779269 1104 78-79 -0.11541538056005507 1104 80-81 -0.01894326799319046 1104 82-83 0.12796409849034518 1104 84-85 0.21267328334881697 1104 86-87 0.23557712869613212 1104 88-89 0.09718161301368511 1104 90-91 0.15960054364321152 1104 92-93 0.3204985701941858 1104 94-95 0.29927697914995477 1104 96-97 0.38880247452659944 1104 98-99 0.5583013842436166 1104 100-101 0.39768365427482166 1105 1 0.2752204743978055 1105 2 0.18699284135367122 1105 3 0.18078944399653807 1105 4 0.04938278833979837 1105 5 0.044530285882821374 1105 6 0.10260527033292277 1105 7 0.06829200788109802 1105 8 0.06351953315515146 1105 9 0.06833692115327494 1105 10-11 0.10773047411613135 1105 12-13 0.16540610675819778 1105 14-15 0.14158852460847982 1105 16-17 0.15389981621835602 1105 18-19 0.17626842704466839 1105 20-21 -0.0016230868860844794 1105 22-23 -0.07934377264722059 1105 24-25 0.009768991462969723 1105 26-27 0.0010920419580955354 1105 28-29 0.001033380001544515 1105 30-31 0.07954282462586804 1105 32-33 0.016825662943226405 1105 34-35 0.007077504908139076 1105 36-37 -0.055693056474382274 1105 38-39 -0.01581657426677907 1105 40-41 0.08463909990521046 1105 42-43 -0.05739217602647528 1105 44-45 -0.03813424630813955 1105 46-47 -0.13423839773616209 1105 48-49 -0.20871173572248125 1105 50-51 -0.1356325713649582 1105 52-53 0.08802255685080951 1105 54-55 -0.12977537299433806 1105 56-57 -0.14092293432760528 1105 58-59 -0.12289286899430607 1105 60-61 -0.17714260734454967 1105 62-63 -0.36414021999636503 1105 64-65 -0.19939613716944393 1105 66-67 -0.2404499763059178 1105 68-69 -0.22170009073565922 1105 70-71 -0.23152152594912678 1105 72-73 -0.2820098187414004 1105 74-75 -0.4887656107310008 1105 76-77 -0.36650839756649845 1105 78-79 -0.34939815182141487 1105 80-81 -0.3736018888129671 1105 82-83 -0.36976490696023845 1105 84-85 -0.4450239262024418 1105 86-87 -0.35849657322649975 1105 88-89 -0.2918438033567199 1105 90-91 -0.2277833160755094 1105 92-93 -0.12469953580228932 1105 94-95 -0.23102672167224725 1105 96-97 -0.328108028531787 1105 98-99 -0.36529155238051914 1105 100-101 -0.2629846167406704 1106 1 0.1814967094596227 1106 2 0.11142566494881123 1106 3 0.16762029735142647 1106 4 0.07411646415064865 1106 5 0.08365756492244003 1106 6 -0.040980545239136745 1106 7 0.058966348648041844 1106 8 0.0684952577554867 1106 9 0.04229004115240542 1106 10-11 -0.011357204830176215 1106 12-13 -0.19101220041918054 1106 14-15 0.08995043691326288 1106 16-17 0.1402951770403007 1106 18-19 0.1131145237231479 1106 20-21 0.21621257815137085 1106 22-23 0.15474268574827477 1106 24-25 0.15394077992252875 1106 26-27 0.21476524896893778 1106 28-29 0.06675684115818825 1106 30-31 0.20874284849441693 1106 32-33 0.02898267460084014 1106 34-35 -0.2297640350472676 1106 36-37 0.20382859609718196 1106 38-39 0.2653376870829902 1106 40-41 0.12956773836720714 1106 42-43 0.042247625864561655 1106 44-45 0.16741519142478722 1106 46-47 0.2492388159361809 1106 48-49 0.18277912642036398 1106 50-51 0.2361584920331623 1106 52-53 0.288166744587798 1106 54-55 0.24650135028780795 1106 56-57 0.3655393669260363 1106 58-59 0.2123231142543176 1106 60-61 0.2761475094485988 1106 62-63 0.0012005849162193272 1106 64-65 -0.2687293909409405 1106 66-67 -0.1960924471347525 1106 68-69 0.266084636647534 1106 70-71 0.027937417433584244 1106 72-73 0.18122350175354995 1106 74-75 0.16520519369336029 1106 76-77 0.12943818028023557 1106 78-79 -0.07385202313738759 1106 80-81 -0.20130426970076698 1106 82-83 -0.22337822561080145 1106 84-85 -0.3061724374016741 1106 86-87 -0.016307284780506137 1106 88-89 0.08027100436584078 1106 90-91 -0.13431244926744057 1106 92-93 -0.18438002272069554 1106 94-95 -0.05348680966039865 1106 96-97 0.09089903981363534 1106 98-99 0.20546231405324633 1106 100-101 -0.13985870471797313 1107 1 0.2308291030192322 1107 2 0.17836714200878845 1107 3 0.03598729367308806 1107 4 0.007562643750219422 1107 5 0.11276749642174622 1107 6 0.05207423328997152 1107 7 0.11875225644742926 1107 8 0.09469115652423454 1107 9 0.04693575380191106 1107 10-11 0.15410412862062373 1107 12-13 0.1426817710792463 1107 14-15 0.201626721576595 1107 16-17 0.0026151699919978455 1107 18-19 0.19760847760081646 1107 20-21 0.1806547710546198 1107 22-23 0.14997076442495683 1107 24-25 0.15445189201257392 1107 26-27 0.12107799538421915 1107 28-29 0.1464495792036118 1107 30-31 0.12969800366720818 1107 32-33 0.1150480696361953 1107 34-35 0.06337234961386429 1107 36-37 0.1942748417745932 1107 38-39 0.11782188346632694 1107 40-41 0.06007951123564936 1107 42-43 0.14992587083713715 1107 44-45 0.05310438329280487 1107 46-47 -0.12500965876701997 1107 48-49 0.063424376524992 1107 50-51 0.02144273687358833 1107 52-53 -0.0021850097788487233 1107 54-55 0.07159088576059247 1107 56-57 0.11270991383028672 1107 58-59 0.1330233190956207 1107 60-61 0.1069272279570086 1107 62-63 0.041147061866062984 1107 64-65 0.09903398431511334 1107 66-67 0.10933584739620983 1107 68-69 0.15868281730434575 1107 70-71 0.10509805266188721 1107 72-73 0.2305067774523195 1107 74-75 0.02370324439708682 1107 76-77 0.145217898788637 1107 78-79 0.12975032042333012 1107 80-81 0.15204103106353273 1107 82-83 0.19734719893931896 1107 84-85 0.25376071385050025 1107 86-87 0.1554036907486669 1107 88-89 0.2278763434552893 1107 90-91 0.3174006187266585 1107 92-93 0.21506899822597703 1107 94-95 0.2962454649591031 1107 96-97 0.2498076541613372 1107 98-99 0.19332634788510106 1107 100-101 0.49089722576814054 1108 1 0.10354182983181914 1108 2 0.0038109437690110326 1108 3 -0.009993765457188886 1108 4 -0.02203579515981602 1108 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quality scores pass #Quality Count 2 1.0 3 7.0 4 16.0 5 55.0 6 90.0 7 212.0 8 516.0 9 1089.0 10 1882.0 11 2934.0 12 4790.0 13 6577.0 14 8788.0 15 12460.0 16 16632.0 17 22875.0 18 20142.0 19 13309.0 20 11542.0 21 11799.0 22 12675.0 23 13806.0 24 16326.0 25 19687.0 26 23746.0 27 29892.0 28 37032.0 29 46753.0 30 57764.0 31 73129.0 32 93566.0 33 123821.0 34 170419.0 35 242283.0 36 364774.0 37 495660.0 38 295603.0 39 15811.0 40 2.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.32434198401662 18.777958349389316 15.216308232507652 22.681391434086414 2 16.053057904794652 19.81022409426639 39.918006228881644 24.218711772057315 3 20.129470809556242 23.436420663311974 29.29306822014005 27.14104030699173 4 13.395181323053254 16.40400006171574 35.62444207867435 34.57637653655666 5 13.846672870076778 37.41913149318433 33.76279453335509 14.9714011033838 6 33.646452557125635 35.29294038038938 16.503141992492722 14.557465069992265 7 30.013731752528695 32.43404681139008 19.267654559360626 18.2845668767206 8 27.326407945460918 35.016233444201255 18.506214554776026 19.1511440555618 9 26.658246876191612 16.82789904186311 19.549651416266066 36.96420266567921 10-11 25.660303332870466 26.538694667980327 26.03346315680427 21.767538842344933 12-13 26.50410138332141 23.568858141555584 27.147203225560435 22.779837249562572 14-15 23.191739788799914 24.984714333260598 25.633523109239064 26.190022768700423 16-17 22.449321457461323 28.41306786747867 26.368579634246064 22.769031040813942 18-19 22.439424897452685 26.609998390982447 28.925793433004255 22.024783278560612 20-21 23.031477232401645 25.452211076653153 29.48416660605299 22.032145084892207 22-23 22.94738011464664 25.29654078547518 29.347106623756282 22.408972476121896 24-25 22.74747064199032 25.599382136170735 29.42087168348938 22.23227553834957 26-27 22.24051182276466 26.030889489546016 29.355396645670606 22.37320204201872 28-29 22.21827678127842 26.379614898514003 29.252865734660944 22.149242585546638 30-31 22.365961123490997 25.92457895537291 29.213829616061965 22.495630305074137 32-33 22.22646150590818 25.8144604391075 29.311120074587883 22.647957980396434 34-35 22.78229992527987 25.950213029515552 29.08733438690921 22.18015265829537 36-37 22.926736802198842 25.64571196822521 29.009374180337804 22.418177049238142 38-39 22.33179705219168 26.03273579270564 29.13794129510484 22.49752585999784 40-41 22.83057789760478 25.888186918368028 28.833627108841238 22.447608075185958 42-43 22.440694764139153 25.88832755862188 28.793024319037414 22.87795335820155 44-45 22.643219093087176 26.063263043511803 28.87613430227048 22.417383561130542 46-47 22.978071074493105 26.524411882043587 28.162413790823308 22.33510325264 48-49 22.981906267013155 26.51632271161336 27.91740229626642 22.584368725107066 50-51 23.067250321252477 26.774052057228126 27.886103598688983 22.27259402283042 52-53 23.22782145635926 26.629571097636507 27.652112772293158 22.49049467371108 54-55 22.88616374644611 26.765682248939754 27.729880718873932 22.6182732857402 56-57 22.96837288651136 26.984987645830994 27.658130057109105 22.388509410548544 58-59 23.096366926534024 26.487095899650203 27.71435750606688 22.702179667748894 60-61 22.73376490269852 26.72688359749875 28.004333326720932 22.5350181730818 62-63 22.621508376809867 26.748021239031676 28.298298629249295 22.332171754909155 64-65 22.68424301989297 26.635814666705166 28.057767710272742 22.62217460312912 66-67 22.63252904497094 26.795079492079445 27.770606996360975 22.80178446658864 68-69 22.78529754701968 26.55943556545946 28.05132545575973 22.603941431761125 70-71 22.76497543493067 26.688443506952943 27.833799507596545 22.712781550519846 72-73 22.820916346516256 26.628491072156724 27.751144496388523 22.799448084938494 74-75 22.633080078378992 27.199009021518954 27.32726755757748 22.840643342524572 76-77 22.935288840691832 26.72959468186637 27.625222341980148 22.709894135461646 78-79 22.940821216108688 26.77120872484257 27.593019949613506 22.694950109435236 80-81 22.904893837903604 26.99629485136425 27.41314060388853 22.685670706843613 82-83 22.82426398742934 27.06831715750335 27.41815855847357 22.68926029659374 84-85 22.78785239168547 27.13105746049197 27.43209829739343 22.648991850429123 86-87 22.824597249682054 27.073175032455865 27.365354986742137 22.73687273111994 88-89 22.775885896410127 27.08904479460781 27.22614190653151 22.908927402450555 90-91 22.888457807953444 26.877457896129087 27.42203068512477 22.8120536107927 92-93 22.765947973548144 26.742829710331804 27.614536618611258 22.876685697508798 94-95 22.65066906476406 26.896623928515538 27.586121011344677 22.866585995375726 96-97 22.752786575944526 26.648725018900443 27.50972573965214 23.088762665502884 98-99 22.519247851492327 26.79340602662188 27.237126036232027 23.45022008565377 100-101 22.61368957286 26.515522944258134 27.770096231937224 23.10069125094464 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 186.0 1 143.0 2 160.0 3 233.0 4 198.0 5 175.0 6 389.0 7 929.5 8 1575.0 9 1943.0 10 1924.5 11 1896.0 12 2066.5 13 2298.5 14 2542.0 15 2753.5 16 2935.5 17 3149.5 18 3374.5 19 3625.0 20 3933.5 21 4400.5 22 5053.5 23 5863.5 24 7006.5 25 8833.0 26 11555.5 27 14509.0 28 16798.0 29 19106.5 30 22433.5 31 26802.0 32 32357.5 33 36116.0 34 39136.0 35 43028.5 36 48189.5 37 53989.5 38 57279.5 39 60280.5 40 63513.0 41 67338.0 42 69970.0 43 72164.5 44 76842.5 45 81409.5 46 84150.5 47 89422.0 48 93518.0 49 91294.0 50 88239.5 51 84284.0 52 81986.5 53 83690.0 54 86255.5 55 80736.5 56 69847.0 57 62988.0 58 58398.5 59 51560.5 60 41689.5 61 34344.5 62 28681.0 63 21549.5 64 15090.5 65 10896.0 66 7770.0 67 5788.5 68 4385.0 69 3307.0 70 2515.0 71 1611.5 72 1118.5 73 904.0 74 650.0 75 455.5 76 325.5 77 228.5 78 166.0 79 104.5 80 68.5 81 54.0 82 35.0 83 18.0 84 11.5 85 6.0 86 2.5 87 1.5 88 1.5 89 1.0 90 0.0 91 0.5 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.01582567947929547 2 0.0 3 0.0 4 0.0 5 0.039453992016627984 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0037029445021192742 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 7.273640986305718E-4 24-25 1.7633069057710832E-4 26-27 0.0010359428071405113 28-29 0.0010579841434626498 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 4.187853901206323E-4 42-43 0.010668006779915053 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 2.204133632213854E-5 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 2.204133632213854E-5 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 3.7470271747635516E-4 84-85 8.816534528855416E-5 86-87 0.0 88-89 0.0 90-91 0.011681908250733425 92-93 0.0018514722510596371 94-95 0.0 96-97 0.0 98-99 0.0013224801793283124 100-101 0.017765317075643664 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2268465.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 47.234907994398384 #Duplication Level Percentage of deduplicated Percentage of total 1 72.53210836265755 34.26047465149863 2 14.376663006571375 13.581607087637396 3 5.191668579436101 7.356839630612197 4 2.4831940523555414 4.691737703810051 5 1.350033503711973 3.1884354168595164 6 0.8512347015980872 2.412479568697489 7 0.5632961856122015 1.8625070450691523 8 0.39560821776319643 1.4949214214298006 9 0.3034857028424831 1.2901607326242017 >10 1.7325636376625178 15.24874109201996 >50 0.14071604019379647 4.617639387260032 >100 0.07335022847369459 6.50157839436849 >500 0.004020685972648013 1.2743412716689795 >1k 0.00177658217396072 1.2445729280962015 >5k 2.8051297483590315E-4 0.9739636683479695 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9145 0.4031360413319139 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7895 0.34803270052656754 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5012 0.22094235529311673 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 8.816534528855416E-5 9 0.0 0.0 0.0 0.0 1.3224801793283123E-4 10-11 0.0 0.0 0.0 0.0 1.3224801793283123E-4 12-13 0.0 0.0 0.0 0.0 1.3224801793283123E-4 14-15 0.0 0.0 0.0 0.0 1.5428935425496976E-4 16-17 0.0 0.0 0.0 2.204133632213854E-5 1.7633069057710832E-4 18-19 0.0 0.0 0.0 4.408267264427708E-5 1.7633069057710832E-4 20-21 4.408267264427708E-5 0.0 0.0 4.408267264427708E-5 1.7633069057710832E-4 22-23 4.408267264427708E-5 0.0 0.0 6.612400896641562E-5 2.4245469954352393E-4 24-25 4.408267264427708E-5 0.0 0.0 1.3224801793283123E-4 3.085787085099395E-4 26-27 4.408267264427708E-5 0.0 0.0 3.3062004483207805E-4 3.085787085099395E-4 28-29 4.408267264427708E-5 0.0 0.0 8.816534528855416E-4 3.085787085099395E-4 30-31 4.408267264427708E-5 0.0 0.0 0.002138009623247438 3.085787085099395E-4 32-33 4.408267264427708E-5 0.0 0.0 0.004650721963971232 3.085787085099395E-4 34-35 4.408267264427708E-5 0.0 0.0 0.0067887315872186705 3.085787085099395E-4 36-37 4.408267264427708E-5 0.0 0.0 0.008574079829311892 3.085787085099395E-4 38-39 4.408267264427708E-5 0.0 0.0 0.010469634753015804 3.085787085099395E-4 40-41 4.408267264427708E-5 0.0 0.0 0.012585603039941107 3.085787085099395E-4 42-43 4.408267264427708E-5 0.0 0.0 0.01536281141653056 3.085787085099395E-4 44-45 4.408267264427708E-5 0.0 0.0 0.018492681174274234 3.3062004483207805E-4 46-47 4.408267264427708E-5 0.0 0.0 0.02237195636697062 3.7470271747635516E-4 48-49 4.408267264427708E-5 0.0 0.0 0.028719861227746515 3.967440537984937E-4 50-51 4.408267264427708E-5 0.0 0.0 0.03647841161313928 3.967440537984937E-4 52-53 4.408267264427708E-5 0.0 0.0 0.052282049756112614 4.187853901206322E-4 54-55 4.408267264427708E-5 0.0 0.0 0.07264824451776863 4.6286806276490933E-4 56-57 4.408267264427708E-5 0.0 0.0 0.0964749290820004 5.73074744375602E-4 58-59 4.408267264427708E-5 0.0 0.0 0.12435721952950564 5.951160806977405E-4 60-61 4.408267264427708E-5 0.0 0.0 0.16610351052363603 6.17157417019879E-4 62-63 4.408267264427708E-5 0.0 0.0 0.20006920979605153 6.17157417019879E-4 64-65 4.408267264427708E-5 0.0 0.0 0.23233772617166232 6.17157417019879E-4 66-67 4.408267264427708E-5 0.0 0.0 0.28616266947032465 6.612400896641562E-4 68-69 4.408267264427708E-5 0.0 0.0 0.35766476449934204 6.832814259862947E-4 70-71 4.408267264427708E-5 0.0 0.0 0.4246483855823211 7.714467712748489E-4 72-73 4.408267264427708E-5 0.0 0.0 0.49473983508672165 8.816534528855415E-4 74-75 4.408267264427708E-5 0.0 0.0 0.5960858994959146 9.257361255298187E-4 76-77 4.408267264427708E-5 0.0 0.0 0.6877778585960109 9.698187981740957E-4 78-79 4.408267264427708E-5 0.0 0.0 0.7801751404584156 9.698187981740957E-4 80-81 4.408267264427708E-5 0.0 0.0 0.8879352337373511 9.698187981740957E-4 82-83 4.408267264427708E-5 0.0 0.0 1.0362513858490212 9.698187981740957E-4 84-85 4.408267264427708E-5 0.0 0.0 1.190849318812501 0.0010579841434626498 86-87 4.408267264427708E-5 0.0 0.0 1.3668273480084552 0.0010579841434626498 88-89 4.408267264427708E-5 0.0 2.204133632213854E-5 1.589863630252175 0.001102066816106927 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 7355 0.0 37.54573 1 GTATCAA 10955 0.0 34.752594 1 ATCAACG 13915 0.0 27.08198 3 TATCAAC 14125 0.0 26.81392 2 TCAACGC 14095 0.0 26.736132 4 CAACGCA 14340 0.0 26.31248 5 AACGCAG 14610 0.0 25.878141 6 GTACATG 10485 0.0 24.750916 1 TACATGG 10275 0.0 24.743572 2 ACATGGG 10235 0.0 23.540222 3 TAAGGTG 1145 0.0 22.411901 5 ACGCAGA 16840 0.0 22.396357 7 GTAAGGT 1065 0.0 22.310581 4 CGCAGAG 16930 0.0 22.137012 8 ATCGGAC 305 9.094947E-12 21.803545 8 GAGTACT 9490 0.0 21.698105 12-13 GTACTTT 9670 0.0 21.07177 14-15 AGTACTT 9690 0.0 20.784569 12-13 GCAGAGT 17935 0.0 20.552246 9 TAGCGCG 185 6.5310724E-6 20.549852 4 >>END_MODULE