FastQCFastQC Report
Thu 26 May 2016
SRR937614_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937614_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2279581
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT75350.3305432007022343No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59640.26162702707208035No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36310.15928365783010123No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN30750.1348932106382708No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA71700.035.0876731
GTATCAA108950.035.0514831
TATCAAC132800.028.4568252
ATCAACG132700.028.2972933
TCAACGC135300.027.7226544
CAACGCA137150.027.2775785
AACGCAG140250.026.6938636
GTACATG111300.025.2101231
TACATGG110900.024.3035032
ACATGGG110950.023.648333
CGCAGAG157200.023.5446438
ACGCAGA158600.023.4855637
CGTATAG1252.2464864E-422.8154963
GAGTACT77750.022.66628812-13
GTACTTT79400.021.9554814-15
GCAGAGT166000.021.4950799
GGACCGA6050.021.2029616
AGTACTT80150.021.06895412-13
CCGTTGC2309.005271E-820.6575769
AGGACCG7550.020.1479075
Produced by FastQC (version 0.11.5)