##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937614_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2279581 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.569272160103107 31.0 31.0 34.0 30.0 34.0 2 31.68268554615958 33.0 31.0 34.0 30.0 34.0 3 31.734183167871638 33.0 31.0 34.0 30.0 34.0 4 35.33653465264011 37.0 35.0 37.0 33.0 37.0 5 35.30746483673973 37.0 35.0 37.0 33.0 37.0 6 35.399430860320386 37.0 35.0 37.0 33.0 37.0 7 35.33135914012268 37.0 35.0 37.0 33.0 37.0 8 35.31047065228215 37.0 35.0 37.0 33.0 37.0 9 36.9010160200493 39.0 37.0 39.0 33.0 39.0 10-11 36.78062130716127 39.0 37.0 39.0 33.0 39.0 12-13 36.64380932285363 39.0 37.0 39.0 32.0 39.0 14-15 37.747109227529094 40.0 37.5 41.0 32.5 41.0 16-17 37.67159535019813 40.0 37.0 41.0 32.0 41.0 18-19 37.60551939150221 40.0 37.0 41.0 32.0 41.0 20-21 37.41669061112547 40.0 37.0 41.0 32.0 41.0 22-23 37.26598572281485 39.5 37.0 41.0 31.5 41.0 24-25 37.105563259212985 39.0 36.5 41.0 31.0 41.0 26-27 37.203221556944015 39.0 37.0 41.0 31.0 41.0 28-29 37.143410784701224 39.0 37.0 41.0 31.0 41.0 30-31 36.93658505663979 39.0 36.0 41.0 30.5 41.0 32-33 36.691040371015546 39.0 36.0 41.0 30.0 41.0 34-35 36.4134590962111 39.0 35.0 40.0 30.0 41.0 36-37 36.435907958523956 39.0 35.0 41.0 30.0 41.0 38-39 36.37095501322392 39.0 35.0 41.0 30.0 41.0 40-41 36.17366809953233 39.0 35.0 41.0 29.5 41.0 42-43 35.91663643450266 39.0 35.0 40.0 29.0 41.0 44-45 35.68022719964765 39.0 35.0 40.0 27.5 41.0 46-47 35.314380362005124 38.5 35.0 40.0 26.5 41.0 48-49 34.95822741109002 38.0 34.0 40.0 24.5 41.0 50-51 34.05088764119371 37.5 33.5 39.5 23.5 40.5 52-53 34.14305348219695 38.0 33.5 39.5 24.0 40.5 54-55 34.42752944510417 38.0 34.0 40.0 23.5 41.0 56-57 34.114314209497266 38.0 33.5 40.0 22.5 41.0 58-59 33.84179921661042 38.0 33.0 40.0 22.0 41.0 60-61 33.49460800032988 37.0 33.0 40.0 21.5 41.0 62-63 33.09922569103708 37.0 32.0 39.5 19.5 41.0 64-65 32.69502750724804 36.0 32.0 39.0 18.0 41.0 66-67 32.23628443121784 35.5 31.0 39.0 15.5 40.0 68-69 31.72246281224488 35.0 31.0 38.5 12.0 40.0 70-71 31.244419478842822 35.0 30.5 38.0 8.0 40.0 72-73 30.750989984562953 35.0 30.0 37.0 7.0 39.0 74-75 30.23710278336238 34.0 29.5 36.5 3.5 39.0 76-77 29.746694458323702 34.0 29.0 36.0 2.0 39.0 78-79 29.181997261777493 34.0 29.0 35.5 2.0 37.5 80-81 28.63818504365495 34.0 27.5 35.0 2.0 37.0 82-83 28.102301256239635 33.5 26.5 35.0 2.0 36.5 84-85 27.605985924606323 33.0 26.0 35.0 2.0 36.0 86-87 27.046663619323024 33.0 25.5 35.0 2.0 35.5 88-89 26.519390186178953 32.0 25.0 35.0 2.0 35.0 90-91 25.965663646082326 32.0 23.5 34.5 2.0 35.0 92-93 25.346006130073903 32.0 20.0 34.0 2.0 35.0 94-95 24.647343305633797 31.0 17.5 34.0 2.0 35.0 96-97 23.79091552351068 31.0 4.5 34.0 2.0 35.0 98-99 22.758807649300465 31.0 2.0 34.0 2.0 35.0 100-101 20.203878475912898 27.5 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.28404927267472857 1101 2 0.36079491174900724 1101 3 0.36317186130586876 1101 4 0.3764706067017869 1101 5 0.3803157408917457 1101 6 0.3384163471405017 1101 7 0.35109197566553974 1101 8 0.3437151501842166 1101 9 0.43863381061238016 1101 10-11 0.3588272186951258 1101 12-13 0.4320909215565507 1101 14-15 0.5115653766398367 1101 16-17 0.5245241826643152 1101 18-19 0.5774755041965633 1101 20-21 0.6212817504964576 1101 22-23 0.725462513970605 1101 24-25 0.6560319124096097 1101 26-27 0.5930582850478245 1101 28-29 0.6410269596902296 1101 30-31 0.4469689475383163 1101 32-33 0.4305982116706133 1101 34-35 0.5925400340969915 1101 36-37 0.5921871765354112 1101 38-39 0.5381325209818044 1101 40-41 0.7626357152077006 1101 42-43 0.651528837756274 1101 44-45 0.8090816706805484 1101 46-47 0.69180916715694 1101 48-49 0.7439041921637113 1101 50-51 0.6900224148507874 1101 52-53 0.6206856424518463 1101 54-55 0.6329729274761959 1101 56-57 0.7372822678077924 1101 58-59 0.7959774037270506 1101 60-61 0.8568746750967406 1101 62-63 0.9409630858909708 1101 64-65 0.8680464707911995 1101 66-67 0.9471961265387705 1101 68-69 1.0617748440820947 1101 70-71 1.0703363205882965 1101 72-73 1.064317396991278 1101 74-75 0.9611607560559818 1101 76-77 0.9013452552156274 1101 78-79 0.9841593537950857 1101 80-81 1.1495469950456396 1101 82-83 1.2675358325915766 1101 84-85 1.2779014003515954 1101 86-87 1.4434173237052583 1101 88-89 1.5863316886411702 1101 90-91 1.6050191353073195 1101 92-93 1.8522286548334606 1101 94-95 1.8705088471342357 1101 96-97 1.8239588603085188 1101 98-99 2.2142171105702495 1101 100-101 2.26387032509351 1102 1 0.26677566129067287 1102 2 0.38342530265021324 1102 3 0.3758881528554845 1102 4 0.29239806420627446 1102 5 0.30319351078670564 1102 6 0.3950773788413997 1102 7 0.3341300857921965 1102 8 0.31404374531583557 1102 9 0.4945650595122757 1102 10-11 0.27131105704684444 1102 12-13 0.4958616884383815 1102 14-15 0.6088033746232071 1102 16-17 0.6938465636953879 1102 18-19 0.6679531939544248 1102 20-21 0.6187701682352511 1102 22-23 0.700703416141998 1102 24-25 0.684218209277951 1102 26-27 0.7432048410724761 1102 28-29 0.6890786394330419 1102 30-31 0.6503205793066016 1102 32-33 0.5154021007874192 1102 34-35 0.5913451016392557 1102 36-37 0.6986498897601408 1102 38-39 0.8323019734205559 1102 40-41 0.816123321150009 1102 42-43 0.8750670437103594 1102 44-45 0.9429665808051837 1102 46-47 0.8935194826542059 1102 48-49 0.9928451817526565 1102 50-51 0.7445130377499538 1102 52-53 0.91932753403497 1102 54-55 0.9995714115783088 1102 56-57 0.9847722059274204 1102 58-59 1.106573311348356 1102 60-61 1.1633411414901502 1102 62-63 1.1978085788827144 1102 64-65 1.1857664486127533 1102 66-67 1.0913626337826443 1102 68-69 0.9609852364671951 1102 70-71 1.092178806701142 1102 72-73 1.0690191964445361 1102 74-75 0.977535952684665 1102 76-77 1.0870941783081634 1102 78-79 1.263983795854415 1102 80-81 1.290757100035318 1102 82-83 1.0991123007104058 1102 84-85 1.403537940367933 1102 86-87 1.5475707956382472 1102 88-89 1.454394603563962 1102 90-91 1.6665271411115263 1102 92-93 1.8252630934576004 1102 94-95 1.8616355570631207 1102 96-97 2.1452956069402696 1102 98-99 2.3185679713751277 1102 100-101 2.3616878922513393 1103 1 0.22978841170949948 1103 2 0.2066053004847319 1103 3 0.19791086139327518 1103 4 0.10136161659839615 1103 5 0.18409994971535326 1103 6 0.16814714794048058 1103 7 0.16231439557143545 1103 8 0.21583708537623636 1103 9 0.10237474725690987 1103 10-11 0.2493438347452468 1103 12-13 0.2879651564319232 1103 14-15 0.4698441670386089 1103 16-17 0.3502852328974484 1103 18-19 0.2786119996097298 1103 20-21 0.3513504650174326 1103 22-23 0.15188979766107025 1103 24-25 0.11404316819081117 1103 26-27 0.08549766795437819 1103 28-29 0.2105592329143775 1103 30-31 0.211062441354116 1103 32-33 0.22752141556699712 1103 34-35 0.2299609794892845 1103 36-37 0.3402438287536427 1103 38-39 0.28837254471777385 1103 40-41 0.2927588246616537 1103 42-43 0.3927242238680151 1103 44-45 0.28417718393435365 1103 46-47 0.30630188217102017 1103 48-49 0.20153612860590897 1103 50-51 0.08130437317981887 1103 52-53 0.14592870457316565 1103 54-55 0.21842068100376366 1103 56-57 0.3051499585757327 1103 58-59 0.3265157011377866 1103 60-61 0.2009580595341447 1103 62-63 0.0718825831313552 1103 64-65 0.23821382749346043 1103 66-67 0.3195706220710335 1103 68-69 0.4796920370249609 1103 70-71 0.5231314783945464 1103 72-73 0.47145180436336176 1103 74-75 0.5404193447616841 1103 76-77 0.5257617372285601 1103 78-79 0.5740040600920508 1103 80-81 0.5795355449470705 1103 82-83 0.4221765004304139 1103 84-85 0.5567570068777385 1103 86-87 0.6276953075824885 1103 88-89 0.7954985755690096 1103 90-91 0.9353050290853666 1103 92-93 1.1072903958683078 1103 94-95 0.966159916523992 1103 96-97 1.1792625926297298 1103 98-99 1.3624112649606168 1103 100-101 1.6625593271136445 1104 1 0.108892693271887 1104 2 0.16027754224231927 1104 3 0.22094255945900088 1104 4 0.12145552861677089 1104 5 0.2111003908707758 1104 6 0.23454324664007942 1104 7 0.2881746762404731 1104 8 0.2904021020823748 1104 9 0.2499408681393831 1104 10-11 0.36952594105450487 1104 12-13 0.27633754679891354 1104 14-15 0.458355085359365 1104 16-17 0.3855618454419343 1104 18-19 0.5152844330620425 1104 20-21 0.5010267916019586 1104 22-23 0.5379950759624492 1104 24-25 0.3857025564675567 1104 26-27 0.35146655601091226 1104 28-29 0.37554611783094316 1104 30-31 0.47116734730412446 1104 32-33 0.49821478752870973 1104 34-35 0.4040776476215626 1104 36-37 0.39861845985168287 1104 38-39 0.5827516212201616 1104 40-41 0.7213377302209167 1104 42-43 0.6338745320329195 1104 44-45 0.6349376895227508 1104 46-47 0.6168494520816452 1104 48-49 0.7166802878221716 1104 50-51 0.6547721123557793 1104 52-53 0.6654720921537773 1104 54-55 0.7152882567424967 1104 56-57 0.7617337709312508 1104 58-59 0.8769165123633726 1104 60-61 1.009407473651045 1104 62-63 0.8990643616095184 1104 64-65 0.7991044058633037 1104 66-67 0.9093833720146449 1104 68-69 1.0194879170969173 1104 70-71 0.9940663636334293 1104 72-73 0.8753921427836566 1104 74-75 0.949308884079894 1104 76-77 0.9796566723301474 1104 78-79 0.9539784186229987 1104 80-81 1.102201974417575 1104 82-83 1.2514790627744325 1104 84-85 1.217469577746833 1104 86-87 1.341084466638705 1104 88-89 1.3444317815473852 1104 90-91 1.292646992238037 1104 92-93 1.393434509828431 1104 94-95 1.4256851942881745 1104 96-97 1.6515503790266202 1104 98-99 1.7535983851355503 1104 100-101 1.3977302132105507 1105 1 0.0033348887281619 1105 2 0.15319412644054964 1105 3 0.20897103264584516 1105 4 0.15570758524503248 1105 5 0.23987195898292413 1105 6 0.23617627116774287 1105 7 0.24091901651391368 1105 8 0.2401100093454005 1105 9 0.24532602413976434 1105 10-11 0.33509987851722656 1105 12-13 0.3023732556816441 1105 14-15 0.2478919486008806 1105 16-17 0.3531140287775898 1105 18-19 0.30710210915233915 1105 20-21 0.5118685755830938 1105 22-23 0.3982623514305388 1105 24-25 0.4014951374376139 1105 26-27 0.45593378400111106 1105 28-29 0.30078935237954596 1105 30-31 0.5058795329598595 1105 32-33 0.4195079085549054 1105 34-35 0.5202142340880513 1105 36-37 0.45806728965695953 1105 38-39 0.6235953724845942 1105 40-41 0.6466971680283535 1105 42-43 0.6703655444184307 1105 44-45 0.6736111505919595 1105 46-47 0.6558678355169221 1105 48-49 0.739563659414884 1105 50-51 0.7007448522273094 1105 52-53 0.6792501511049025 1105 54-55 0.6550454377750157 1105 56-57 0.8425979843338496 1105 58-59 1.0156673891199475 1105 60-61 0.9060212548791782 1105 62-63 1.006549585557913 1105 64-65 1.0243712996345948 1105 66-67 0.9611922860204487 1105 68-69 0.9995427140092943 1105 70-71 1.0478107694829788 1105 72-73 0.8188787906704427 1105 74-75 0.9375018955003007 1105 76-77 1.017324267626286 1105 78-79 1.0514937816704801 1105 80-81 1.069981880263036 1105 82-83 1.0578438720426284 1105 84-85 1.190287383195475 1105 86-87 1.0903966649312942 1105 88-89 1.0442034212152294 1105 90-91 1.2375557846390244 1105 92-93 1.4156212598265867 1105 94-95 1.491344819196815 1105 96-97 1.4170008779332512 1105 98-99 1.7756453125160867 1105 100-101 2.017231910515612 1106 1 -0.007752619429975738 1106 2 0.029802760081558688 1106 3 0.11602999551025306 1106 4 0.10951047454886265 1106 5 0.028123862128502708 1106 6 0.014129391167337246 1106 7 0.06451802166204601 1106 8 -0.0951863169095688 1106 9 -0.005681931147343278 1106 10-11 -0.24397799358837347 1106 12-13 -0.3133724548386425 1106 14-15 -0.2445042887020321 1106 16-17 -0.36529570756650287 1106 18-19 -0.3218200968586018 1106 20-21 -0.1919012401878888 1106 22-23 -0.22595679820798154 1106 24-25 -0.2205449388646059 1106 26-27 -0.12513511478254458 1106 28-29 0.09605851423952316 1106 30-31 0.14953899022633266 1106 32-33 0.11658868105183728 1106 34-35 -0.0950205690960857 1106 36-37 0.09833686729849234 1106 38-39 -0.054875710538162537 1106 40-41 -0.22707722133973363 1106 42-43 -0.10367135430473695 1106 44-45 -0.3759572555535371 1106 46-47 -0.04387708879824004 1106 48-49 0.018859564848519028 1106 50-51 -0.04701843199340772 1106 52-53 0.121996715867283 1106 54-55 0.17169150435550762 1106 56-57 0.1073761837729208 1106 58-59 -0.11003565969279805 1106 60-61 -0.18054509503753735 1106 62-63 6.64386625338409E-4 1106 64-65 -0.12848322973848525 1106 66-67 0.15433258331686517 1106 68-69 0.17577456136454828 1106 70-71 -0.28101353887070246 1106 72-73 -0.07315313379339017 1106 74-75 -0.1257331243942197 1106 76-77 -0.05769311960241552 1106 78-79 -0.0882800079642081 1106 80-81 -0.18868716517810924 1106 82-83 -0.3818882764392626 1106 84-85 -0.22836716028897897 1106 86-87 -0.22930060829766674 1106 88-89 0.1395827651875301 1106 90-91 0.4483299446972815 1106 92-93 0.373285562838106 1106 94-95 0.24338466870908704 1106 96-97 0.3057679722249489 1106 98-99 0.42664079435651203 1106 100-101 0.0022870834681398833 1107 1 0.3639503600858518 1107 2 0.3437543429493424 1107 3 0.4526789713016832 1107 4 0.36030749503303383 1107 5 0.3542402494655619 1107 6 0.3768603036440581 1107 7 0.3348876678445407 1107 8 0.2470231802971341 1107 9 0.5337910669904602 1107 10-11 0.5431146507319298 1107 12-13 0.47910566434200064 1107 14-15 0.6605566982625959 1107 16-17 0.6767098790256725 1107 18-19 0.6094629571451193 1107 20-21 0.7814455940765441 1107 22-23 0.7297657343804715 1107 24-25 0.6595579475413516 1107 26-27 0.5347308070051682 1107 28-29 0.5393015309993032 1107 30-31 0.5611800516788392 1107 32-33 0.7632832806794028 1107 34-35 0.776027382486788 1107 36-37 0.7856715972798654 1107 38-39 0.732499522236516 1107 40-41 0.8444274341537508 1107 42-43 0.9273076959745907 1107 44-45 0.8503928375563348 1107 46-47 0.9848547547771815 1107 48-49 0.8742143134518727 1107 50-51 0.9282054972430984 1107 52-53 0.8713752074874037 1107 54-55 0.8621323795882816 1107 56-57 1.015299833679812 1107 58-59 1.0472492565083087 1107 60-61 1.1229237131561263 1107 62-63 1.166906937835762 1107 64-65 1.3112956343210982 1107 66-67 1.3627450600394013 1107 68-69 1.4094882485153875 1107 70-71 1.3282653251888803 1107 72-73 1.2941443522401315 1107 74-75 1.1933543988832476 1107 76-77 1.0661988729487923 1107 78-79 1.164748856095379 1107 80-81 1.3833421644308288 1107 82-83 1.5880707552180908 1107 84-85 1.4710983890592502 1107 86-87 1.4437552576241224 1107 88-89 1.6280464523383706 1107 90-91 1.605129758477542 1107 92-93 1.753847015352072 1107 94-95 2.0073832720610447 1107 96-97 2.091555681722152 1107 98-99 2.1772497329039986 1107 100-101 2.3063005843992386 1108 1 0.15902759585820903 1108 2 0.24419517402431978 1108 3 0.25720878159978966 1108 4 0.3257474227656445 1108 5 0.316011252175592 1108 6 0.2785752991273469 1108 7 0.14819073920858727 1108 8 0.19252318029713678 1108 9 0.2202255746688735 1108 10-11 0.339089131310061 1108 12-13 0.36032224067442087 1108 14-15 0.3535600857964596 1108 16-17 0.34116155020905836 1108 18-19 0.3758459743086249 1108 20-21 0.4607276627305623 1108 22-23 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2041.0 4 2090.0 5 2339.0 6 2607.0 7 3215.0 8 3977.0 9 4798.0 10 5987.0 11 7697.0 12 9665.0 13 11856.0 14 13809.0 15 16459.0 16 20227.0 17 24916.0 18 22205.0 19 17706.0 20 17824.0 21 19041.0 22 20621.0 23 23083.0 24 25758.0 25 29599.0 26 34064.0 27 39884.0 28 46843.0 29 56224.0 30 67370.0 31 83492.0 32 104069.0 33 133417.0 34 175250.0 35 237077.0 36 324779.0 37 391159.0 38 246319.0 39 22707.0 40 6.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.3055683913585 18.012973696139138 14.464703008858903 23.216754903643462 2 15.414384982209128 19.176882469405378 40.938400787909686 24.47033176047581 3 19.826646216562033 23.377205464171777 28.853781018789963 27.942367300476228 4 12.859547786550607 15.74367210411968 35.98612066034196 35.41065944898775 5 13.896346294188882 37.36542028900079 33.60798774986462 15.130245666945704 6 33.409560987618505 34.896923418463004 16.996129270496073 14.697386323422421 7 30.05228150252836 32.24538811565051 19.33307897346452 18.3692514083566 8 27.100526639909027 34.85089378454138 18.736925760959725 19.31165381458987 9 26.74738751855696 17.068270708951697 19.60138089717873 36.582960875312615 10-11 25.51937771168061 26.46371680949091 26.15953160886254 21.857373869965937 12-13 26.409152880704973 23.49028086678417 27.063586312791266 23.036979939719593 14-15 23.27197924414505 24.956392307462366 25.51817104414048 26.2534574042521 16-17 22.556850496972107 28.49511115174285 26.0634552549295 22.884583096355538 18-19 22.57781923291968 26.850074900572128 28.47399754551177 22.098108320996424 20-21 23.298202835389162 25.750935544813526 28.72936214182132 22.221499477975993 22-23 23.01889106293629 25.55925190010595 28.704495091470793 22.717361945486964 24-25 22.704668286525052 25.825767352234806 28.836607334371823 22.63295702686832 26-27 22.271363480924137 26.244508969818124 28.786777924586186 22.69734962467155 28-29 22.34567027471612 26.60391740712786 28.643924071388476 22.406488246767548 30-31 22.493687623522128 26.161833332161116 28.575174425556156 22.769304618760593 32-33 22.265440228735013 26.121167308196164 28.680544687555496 22.932847775513327 34-35 22.832799611412344 26.209446108738604 28.45828726913871 22.499467010710337 36-37 22.869534069083127 25.909929985100405 28.476241962351057 22.744293983465408 38-39 22.414359492260125 26.32601414265665 28.519046617678693 22.74057974740453 40-41 22.869012681947584 26.178017488765192 28.312652327953458 22.64031750133376 42-43 22.410214802338952 26.165453883776777 28.245263729733722 23.17906758415055 44-45 22.624559981610318 26.352689820494035 28.360105468161105 22.66264472973454 46-47 22.956470830491977 26.69009489597616 27.701862999140065 22.6515712743918 48-49 22.88320410619631 26.62629252780237 27.61448668589761 22.87601668010371 50-51 23.032020823392603 26.930274910365824 27.501014861261268 22.536689404980304 52-53 23.01533234366767 26.816510226068917 27.403890647879287 22.764266782384126 54-55 22.77442831417788 26.834675585025565 27.51737572396103 22.873520376835533 56-57 22.964345629749587 27.155242026299398 27.282289491117993 22.598122852833026 58-59 23.021010632103213 26.63292853730685 27.4082199390279 22.93784089156204 60-61 22.67716220568907 26.85746388908541 27.703007034767808 22.762366870457708 62-63 22.667439803232494 26.861712996999888 27.92698034214589 22.54386685762173 64-65 22.59557992622151 26.874921311806077 27.712745960343128 22.816752801629285 66-67 22.592410794914983 26.976499224169864 27.42075041296311 23.01033956795204 68-69 22.63802490685254 26.806979473390818 27.72534742569791 22.829648194058734 70-71 22.703623672769478 26.777107891025697 27.571919073930275 22.94734936227455 72-73 22.712434277412434 26.82951534968075 27.46627930558891 22.991771067317902 74-75 22.58034175806813 27.420504297412457 27.130406157174253 22.868747787345164 76-77 22.903971487209233 26.989215414837375 27.20259990243774 22.904213195515652 78-79 22.888107522397018 27.004052328457252 27.225797605080693 22.882042544065033 80-81 22.9243033035976 27.141880272767743 27.075081444032467 22.858734979602186 82-83 22.791867879291978 27.202409081112716 27.129975831417045 22.875747208178257 84-85 22.85202836434434 27.317389209606546 27.09338848549279 22.737193940556324 86-87 22.88719684138765 27.219633277741394 27.05424157865586 22.838928302215102 88-89 22.808252932198076 27.219990820505735 26.926677636067108 23.045078611229084 90-91 22.925915647754415 27.025492526438764 27.07011834521048 22.978473480596335 92-93 22.86258054281562 26.8533910925787 27.265404699222596 23.01862366538308 94-95 22.73638190665501 27.06142547062379 27.159044793932708 23.043147828788495 96-97 22.848622503608453 26.83210436817846 27.073935003677118 23.245338124535966 98-99 22.704974949459437 26.954117957282236 26.930254021270983 23.41065307198734 100-101 23.164841307087528 26.22436191510716 27.419612113286547 23.191184664518772 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 2624.0 1 1526.0 2 372.5 3 318.5 4 276.5 5 252.0 6 405.5 7 782.0 8 1268.0 9 1659.5 10 1738.0 11 1746.5 12 1910.0 13 2079.5 14 2269.0 15 2444.0 16 2524.5 17 2647.5 18 2885.5 19 3195.5 20 3531.0 21 3968.0 22 4691.0 23 5486.5 24 6614.0 25 8364.5 26 10890.5 27 13868.5 28 16341.5 29 18892.0 30 22138.0 31 26655.5 32 32539.5 33 36414.5 34 39473.0 35 43300.5 36 48357.5 37 54349.5 38 57968.0 39 60296.0 40 63199.5 41 67319.5 42 70315.5 43 72700.0 44 76643.0 45 81166.0 46 84748.5 47 89144.5 48 92718.0 49 91704.0 50 88928.5 51 85130.5 52 83044.0 53 84181.5 54 85570.5 55 80105.0 56 70419.5 57 63707.0 58 59172.0 59 52663.0 60 43099.0 61 35386.0 62 29106.0 63 22309.5 64 16213.0 65 12056.0 66 8736.5 67 6350.5 68 4704.0 69 3530.5 70 2713.0 71 1944.0 72 1390.5 73 1035.5 74 771.5 75 572.5 76 412.5 77 273.0 78 189.5 79 120.5 80 84.5 81 62.0 82 41.0 83 31.0 84 22.0 85 16.5 86 10.5 87 6.0 88 10.5 89 11.5 90 7.5 91 7.0 92 5.0 93 5.0 94 5.5 95 4.0 96 3.0 97 1.5 98 0.5 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.319839479272726 2 0.27303263187401544 3 0.26671568152217445 4 0.2752260174128491 5 0.27636657789304264 6 0.21126689510045926 7 0.20130892475415438 8 0.2014405278864844 9 0.21179330762977933 10-11 0.21124496124507092 12-13 0.20672658703507354 14-15 0.20850322932152882 16-17 0.1931495305496931 18-19 0.20025609969551422 20-21 0.18874082561663744 22-23 0.19786530945818553 24-25 0.19674668283338037 26-27 0.19771177247046717 28-29 0.2088103032969655 30-31 0.20552022498871503 32-33 0.21095982112502254 34-35 0.20512541559172498 36-37 0.1908684095893061 38-39 0.18093237309838955 40-41 0.17823450888562417 42-43 0.19183349922639292 44-45 0.1926669857311497 46-47 0.1932591998266348 48-49 0.20946831895861562 50-51 0.20352424414837642 52-53 0.22265056604700598 54-55 0.19407075247600325 56-57 0.17781776563324575 58-59 0.1804717621352345 60-61 0.1703821886565996 62-63 0.17555857852824708 64-65 0.17483476130043196 66-67 0.17345292841096674 68-69 0.17645786659916887 70-71 0.17862931828261422 72-73 0.17601918949140216 74-75 0.18935497356751088 76-77 0.18034015900290448 78-79 0.18523140875450358 80-81 0.1795066724981477 82-83 0.16198152204286664 84-85 0.1621131251751967 86-87 0.16544707119422386 88-89 0.1691758266102411 90-91 0.17514183527586868 92-93 0.17231236793077326 94-95 0.17128147672752142 96-97 0.1764139988883922 98-99 0.18341089875727162 100-101 0.1695487021518428 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2279581.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 49.66338772784961 #Duplication Level Percentage of deduplicated Percentage of total 1 74.1734940727344 36.83706995263563 2 13.81526444271268 13.72225669162428 3 4.816695019859648 7.176401770142761 4 2.249082119204375 4.467881492712822 5 1.2548770263895928 3.1160722156178657 6 0.7542887175861521 2.2476319824134117 7 0.522479313445957 1.816366490641306 8 0.37708900627519426 1.498201402124359 9 0.268962464236822 1.2021828431068058 >10 1.5687347530199842 14.532353663584463 >50 0.1281617657792866 4.3709413650985205 >100 0.06510956278845575 5.863698849603877 >500 0.004166178314945149 1.4008892693169368 >1k 0.0014182734689174685 1.153790046493841 >5k 1.7728418361468356E-4 0.5942619648830693 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7535 0.3305432007022343 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5964 0.26162702707208035 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3631 0.15928365783010123 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 3075 0.1348932106382708 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 4.386771077667343E-5 4.386771077667343E-5 5 0.0 0.0 0.0 4.386771077667343E-5 4.386771077667343E-5 6 0.0 0.0 0.0 4.386771077667343E-5 4.386771077667343E-5 7 0.0 0.0 0.0 4.386771077667343E-5 4.386771077667343E-5 8 0.0 0.0 0.0 4.386771077667343E-5 4.386771077667343E-5 9 0.0 0.0 0.0 4.386771077667343E-5 4.386771077667343E-5 10-11 0.0 0.0 0.0 4.386771077667343E-5 4.386771077667343E-5 12-13 0.0 0.0 0.0 4.386771077667343E-5 6.580156616501015E-5 14-15 0.0 0.0 0.0 4.386771077667343E-5 1.0966927694168358E-4 16-17 0.0 0.0 0.0 4.386771077667343E-5 1.316031323300203E-4 18-19 0.0 0.0 0.0 4.386771077667343E-5 1.7547084310669373E-4 20-21 0.0 0.0 0.0 4.386771077667343E-5 1.7547084310669373E-4 22-23 0.0 0.0 0.0 4.386771077667343E-5 2.4127240927170388E-4 24-25 2.1933855388336716E-5 0.0 0.0 2.1933855388336717E-4 3.2900783082505077E-4 26-27 4.386771077667343E-5 0.0 0.0 3.948093969900609E-4 3.5094168621338745E-4 28-29 4.386771077667343E-5 0.0 0.0 0.0010308912032518257 3.5094168621338745E-4 30-31 4.386771077667343E-5 0.0 0.0 0.0024127240927170387 3.5094168621338745E-4 32-33 4.386771077667343E-5 0.0 0.0 0.004693845053104058 3.5094168621338745E-4 34-35 4.386771077667343E-5 0.0 0.0 0.007391709265869474 3.7287554160172413E-4 36-37 4.386771077667343E-5 0.0 0.0 0.009475425527761462 3.9480939699006087E-4 38-39 4.386771077667343E-5 0.0 0.0 0.011032729260333368 3.9480939699006087E-4 40-41 4.386771077667343E-5 0.0 0.0 0.01355512262999209 3.9480939699006087E-4 42-43 4.386771077667343E-5 0.0 0.0 0.01704260563673763 3.9480939699006087E-4 44-45 4.386771077667343E-5 0.0 0.0 0.02017914695726978 4.606109631550711E-4 46-47 4.386771077667343E-5 0.0 0.0 0.024894925865762176 5.264125293200812E-4 48-49 4.386771077667343E-5 0.0 0.0 0.0304661251343997 5.264125293200812E-4 50-51 4.386771077667343E-5 0.0 0.0 0.038910659458909334 5.702802400967546E-4 52-53 4.386771077667343E-5 0.0 0.0 0.057291230274335504 5.702802400967546E-4 54-55 4.386771077667343E-5 0.0 0.0 0.08069465397369077 5.702802400967546E-4 56-57 4.386771077667343E-5 0.0 0.0 0.10881385658153844 5.702802400967546E-4 58-59 4.386771077667343E-5 0.0 0.0 0.13930191557132648 5.702802400967546E-4 60-61 4.386771077667343E-5 0.0 0.0 0.18516560718833858 5.702802400967546E-4 62-63 4.386771077667343E-5 0.0 0.0 0.21966756171419222 5.702802400967546E-4 64-65 4.386771077667343E-5 0.0 0.0 0.254827531901696 5.702802400967546E-4 66-67 4.386771077667343E-5 0.0 0.0 0.3154307743396703 5.702802400967546E-4 68-69 4.386771077667343E-5 0.0 0.0 0.39184832651263546 5.702802400967546E-4 70-71 4.386771077667343E-5 0.0 0.0 0.46113737568439117 6.141479508734281E-4 72-73 4.386771077667343E-5 0.0 0.0 0.5373575231588612 7.018833724267749E-4 74-75 4.386771077667343E-5 0.0 0.0 0.6415872039642373 7.018833724267749E-4 76-77 4.386771077667343E-5 0.0 0.0 0.7357711790017551 7.018833724267749E-4 78-79 4.386771077667343E-5 0.0 0.0 0.8276301653681093 7.018833724267749E-4 80-81 4.386771077667343E-5 0.0 0.0 0.9359176094203276 7.238172278151117E-4 82-83 4.386771077667343E-5 0.0 0.0 1.088489507501598 7.457510832034484E-4 84-85 4.386771077667343E-5 0.0 0.0 1.2506903680983479 7.457510832034484E-4 86-87 4.386771077667343E-5 0.0 0.0 1.4373036097423166 7.457510832034484E-4 88-89 4.386771077667343E-5 0.0 0.0 1.6669949433689788 7.457510832034484E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 7170 0.0 35.087673 1 GTATCAA 10895 0.0 35.051483 1 TATCAAC 13280 0.0 28.456825 2 ATCAACG 13270 0.0 28.297293 3 TCAACGC 13530 0.0 27.722654 4 CAACGCA 13715 0.0 27.277578 5 AACGCAG 14025 0.0 26.693863 6 GTACATG 11130 0.0 25.210123 1 TACATGG 11090 0.0 24.303503 2 ACATGGG 11095 0.0 23.64833 3 CGCAGAG 15720 0.0 23.544643 8 ACGCAGA 15860 0.0 23.485563 7 CGTATAG 125 2.2464864E-4 22.815496 3 GAGTACT 7775 0.0 22.666288 12-13 GTACTTT 7940 0.0 21.95548 14-15 GCAGAGT 16600 0.0 21.495079 9 GGACCGA 605 0.0 21.202961 6 AGTACTT 8015 0.0 21.068954 12-13 CCGTTGC 230 9.005271E-8 20.657576 9 AGGACCG 755 0.0 20.147907 5 >>END_MODULE