FastQCFastQC Report
Thu 26 May 2016
SRR937614_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937614_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2279581
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT93410.40976828636490653No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT76800.336904018764852No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50800.22284797074550103No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22940.10063252852168884No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA83900.033.300451
GTATCAA122450.032.905751
GGACCGA6150.028.5758656
TATCAAC153350.026.1472912
ATCAACG153000.026.1139533
TCAACGC154650.025.9582124
CAACGCA157000.025.5696665
AGGACCG6800.025.1513655
AACGCAG160650.025.1015016
TACATGG103850.023.2394182
GTACATG105350.023.2277641
ACATGGG104200.022.5230523
GAGTACT101250.022.3317512-13
CGCAGAG182900.021.712188
ACGCAGA184650.021.6864837
GTATAGG5950.021.5616261
GTACTTT103450.021.4876114-15
AGTACTT104100.020.99026712-13
TAAGGTG11150.020.4519355
GCAGAGT193400.020.3123389
Produced by FastQC (version 0.11.5)