##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937614_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2279581 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.13351620319699 33.0 31.0 34.0 31.0 34.0 2 32.342743249746334 34.0 31.0 34.0 31.0 34.0 3 32.43780721106203 34.0 31.0 34.0 31.0 34.0 4 36.08489323257213 37.0 35.0 37.0 35.0 37.0 5 35.9940015292284 37.0 35.0 37.0 35.0 37.0 6 36.029909005207536 37.0 35.0 37.0 35.0 37.0 7 35.97755026033293 37.0 35.0 37.0 35.0 37.0 8 35.9446929062841 37.0 35.0 37.0 35.0 37.0 9 37.61628474706536 39.0 37.0 39.0 35.0 39.0 10-11 37.53923264845601 39.0 37.0 39.0 35.0 39.0 12-13 37.43479635073288 39.0 37.0 39.0 34.5 39.0 14-15 38.63631978859273 40.0 38.0 41.0 34.0 41.0 16-17 38.584750223834995 40.0 38.0 41.0 34.0 41.0 18-19 38.56539754454876 40.0 38.0 41.0 34.0 41.0 20-21 38.441591678470736 40.0 38.0 41.0 34.0 41.0 22-23 38.28044846838081 40.0 38.0 41.0 34.0 41.0 24-25 38.358806728078534 40.0 38.0 41.0 34.0 41.0 26-27 38.23388223537572 40.0 38.0 41.0 34.0 41.0 28-29 38.09077962134269 40.0 38.0 41.0 33.0 41.0 30-31 38.13933306164598 40.0 38.0 41.0 34.0 41.0 32-33 38.067247007235096 40.0 37.0 41.0 33.5 41.0 34-35 37.9360965896803 40.0 37.0 41.0 33.0 41.0 36-37 37.76839033138107 40.0 37.0 41.0 33.0 41.0 38-39 37.60710082247571 40.0 37.0 41.0 33.0 41.0 40-41 37.42847676831839 40.0 37.0 41.0 32.5 41.0 42-43 37.195350812276466 40.0 36.0 41.0 32.0 41.0 44-45 36.87911945221512 39.0 36.0 41.0 31.0 41.0 46-47 36.52816921179813 39.0 36.0 41.0 30.5 41.0 48-49 36.1934096660746 39.0 35.0 40.5 30.0 41.0 50-51 35.92186085951761 39.0 35.0 40.0 29.0 41.0 52-53 35.650235503805305 39.0 35.0 40.0 28.0 41.0 54-55 35.41641534124034 38.0 35.0 40.0 28.0 41.0 56-57 35.125326101594986 38.0 34.0 40.0 27.0 41.0 58-59 34.82286678999343 38.0 34.0 40.0 26.5 41.0 60-61 34.43451603606101 37.5 34.0 40.0 26.0 41.0 62-63 34.05562557329615 37.0 33.0 39.5 26.0 41.0 64-65 33.63134124209668 36.0 33.0 39.0 25.0 40.5 66-67 33.157061100263604 36.0 32.0 39.0 23.5 40.0 68-69 32.68684990794361 35.0 32.0 38.5 22.5 40.0 70-71 32.247762198404004 35.0 31.0 38.0 22.0 40.0 72-73 31.73162743504179 35.0 31.0 37.0 20.5 39.0 74-75 31.21145574559535 34.0 30.5 36.5 18.5 39.0 76-77 30.01514225640589 33.5 29.5 35.0 18.5 37.5 78-79 30.620934505069133 34.0 30.0 35.5 18.0 37.5 80-81 30.42756695199688 34.0 30.0 35.0 17.5 37.0 82-83 30.05438674914381 34.0 30.0 35.0 13.5 36.5 84-85 29.65097445539334 34.0 29.5 35.0 8.5 36.0 86-87 29.21295733733524 34.0 29.0 35.0 6.0 36.0 88-89 28.795765537614148 34.0 29.0 35.0 2.0 35.0 90-91 28.21411237415999 33.0 28.0 35.0 2.0 35.0 92-93 27.678407128327528 33.0 27.0 35.0 2.0 35.0 94-95 27.14108689272283 33.0 26.0 34.5 2.0 35.0 96-97 26.49437352741578 32.0 25.0 34.0 2.0 35.0 98-99 25.49549105734782 32.0 22.0 34.0 2.0 35.0 100-101 22.73633049231416 29.0 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.2398346696525806 1101 2 0.20635620763295037 1101 3 0.15010961868835437 1101 4 0.15713639900867094 1101 5 0.10754967956174966 1101 6 0.15390287274988168 1101 7 0.16534153433523358 1101 8 0.170186282764206 1101 9 0.27287950207678335 1101 10-11 0.2806833770847419 1101 12-13 0.31846454536395896 1101 14-15 0.3134382058555971 1101 16-17 0.34689367329534804 1101 18-19 0.35749000285428423 1101 20-21 0.30598369051635643 1101 22-23 0.2936712755907962 1101 24-25 0.34228293392712317 1101 26-27 0.2948434751459743 1101 28-29 0.3790060745458561 1101 30-31 0.44803985233278354 1101 32-33 0.44251608964159317 1101 34-35 0.39080331388696266 1101 36-37 0.37333076691917455 1101 38-39 0.3809032350872883 1101 40-41 0.33222864232354965 1101 42-43 0.3696210115247851 1101 44-45 0.5851819827856488 1101 46-47 0.5893206702290712 1101 48-49 0.6949629048300139 1101 50-51 0.6190092009965866 1101 52-53 0.6659249146935267 1101 54-55 0.6679141473455061 1101 56-57 0.6139928689108203 1101 58-59 0.5200257330752436 1101 60-61 0.647016913003327 1101 62-63 0.5827723317097124 1101 64-65 0.6800251993022002 1101 66-67 0.6664529720818422 1101 68-69 0.6037047252567334 1101 70-71 0.6673108173527211 1101 72-73 0.6235358599133711 1101 74-75 0.48293671831161333 1101 76-77 0.478074655379654 1101 78-79 0.4947763036526496 1101 80-81 0.5582389184753787 1101 82-83 0.4948387090244033 1101 84-85 0.474801264435996 1101 86-87 0.4846924493530409 1101 88-89 0.5829963785054773 1101 90-91 0.688203134483004 1101 92-93 0.7673414997020309 1101 94-95 0.6241331611981913 1101 96-97 0.9354377933770408 1101 98-99 1.015850612833617 1101 100-101 1.3707169308657754 1102 1 0.36201907638347564 1102 2 0.34239328198930963 1102 3 0.30746399617754605 1102 4 0.23533853390137693 1102 5 0.1891786659785737 1102 6 0.20300248584336344 1102 7 0.2899081606379639 1102 8 0.22017397202338174 1102 9 0.3085394698586086 1102 10-11 0.36193035695681175 1102 12-13 0.32554465483832473 1102 14-15 0.4606573771802758 1102 16-17 0.45437095837127117 1102 18-19 0.4564866036302462 1102 20-21 0.36627539165223055 1102 22-23 0.46422507821965553 1102 24-25 0.376154499451971 1102 26-27 0.4584646018020422 1102 28-29 0.46716392378559135 1102 30-31 0.4429819362484224 1102 32-33 0.3586650883535256 1102 34-35 0.3837077399675195 1102 36-37 0.5071027344674661 1102 38-39 0.559574655072602 1102 40-41 0.4523722635787877 1102 42-43 0.5625448289769182 1102 44-45 0.5577551813037758 1102 46-47 0.4219386426596188 1102 48-49 0.41658977487671933 1102 50-51 0.3506698026469053 1102 52-53 0.4352077310713085 1102 54-55 0.3151198524399277 1102 56-57 0.2734172231877139 1102 58-59 0.3571178822282519 1102 60-61 0.3566568399068899 1102 62-63 0.42203263081292874 1102 64-65 0.5442845557693019 1102 66-67 0.42673878282796807 1102 68-69 0.19746200679495018 1102 70-71 0.29803628922245906 1102 72-73 0.4940574478442876 1102 74-75 0.43702842803111963 1102 76-77 0.2862401256369189 1102 78-79 0.5976121701142105 1102 80-81 0.45675888420339206 1102 82-83 0.49018451921102724 1102 84-85 0.5340699664668946 1102 86-87 0.7238785886230552 1102 88-89 0.8334632223008569 1102 90-91 0.8093334394609712 1102 92-93 0.7756652806997728 1102 94-95 0.876405174879185 1102 96-97 1.136711194861661 1102 98-99 0.8570932279095054 1102 100-101 1.2144686087146255 1103 1 0.2327332056906144 1103 2 0.2118165952717206 1103 3 0.14413209612333588 1103 4 0.14565008358282938 1103 5 0.10378434529234681 1103 6 0.1310315794494059 1103 7 0.10851780839376346 1103 8 0.06770720409318187 1103 9 0.19263826995145905 1103 10-11 0.10504006646007014 1103 12-13 0.16412597486419145 1103 14-15 0.24707220874807234 1103 16-17 0.22692011160080483 1103 18-19 0.13325324699315644 1103 20-21 0.1554149813930863 1103 22-23 0.2544657445802869 1103 24-25 0.2019654235295718 1103 26-27 0.1776025449438663 1103 28-29 0.19269402662538226 1103 30-31 0.2539068786607004 1103 32-33 0.27749415580770886 1103 34-35 0.3060856434387631 1103 36-37 0.15980767118582406 1103 38-39 0.3192406410758153 1103 40-41 0.32759819529958634 1103 42-43 0.35695042229393437 1103 44-45 0.33379825508905725 1103 46-47 0.42447694770730493 1103 48-49 0.17797223659069772 1103 50-51 0.23956440889353559 1103 52-53 0.43529161768152136 1103 54-55 0.4789197014094384 1103 56-57 0.30248367418219146 1103 58-59 0.3033232152027452 1103 60-61 0.29497008600046115 1103 62-63 0.3241101475755528 1103 64-65 0.35997927354187453 1103 66-67 0.3402947552605724 1103 68-69 0.2952225110416933 1103 70-71 0.5324809442962888 1103 72-73 0.5147150877351514 1103 74-75 0.4262908554432556 1103 76-77 0.3357660884562996 1103 78-79 0.47880745572130934 1103 80-81 0.4444434650142135 1103 82-83 0.5616650780999457 1103 84-85 0.5475468260427867 1103 86-87 0.6117722632959044 1103 88-89 0.5749981120392604 1103 90-91 0.5525783188320261 1103 92-93 0.7433481803191562 1103 94-95 0.865479073620957 1103 96-97 0.8829064614429321 1103 98-99 0.9326147864224019 1103 100-101 1.134130575079034 1104 1 0.2664440660246399 1104 2 0.248101720754498 1104 3 0.17705796471935997 1104 4 0.07368067232111741 1104 5 0.09877912611830908 1104 6 0.14044191396590122 1104 7 0.12970081136337086 1104 8 0.20396315224068218 1104 9 0.28009632063319145 1104 10-11 0.16756280951224767 1104 12-13 0.24938165184276073 1104 14-15 0.30245616297975886 1104 16-17 0.28830657589278985 1104 18-19 0.23518397344994213 1104 20-21 0.24965271523029742 1104 22-23 0.2933690115955869 1104 24-25 0.16630818105635115 1104 26-27 0.15291311063463553 1104 28-29 0.05374423408616735 1104 30-31 0.21290738751636695 1104 32-33 0.23709624023948095 1104 34-35 0.26425995170964 1104 36-37 0.26378016722407693 1104 38-39 0.21077647272001343 1104 40-41 0.2703702371574721 1104 42-43 0.2770708441106393 1104 44-45 0.20631785964601335 1104 46-47 0.23673928396192423 1104 48-49 0.072935945210304 1104 50-51 0.17696035195615423 1104 52-53 -0.02024826788298384 1104 54-55 -0.06014288195108719 1104 56-57 0.07494056402541815 1104 58-59 0.09291728230620322 1104 60-61 -0.0011609183952714375 1104 62-63 0.027625924492930665 1104 64-65 0.10416211130817032 1104 66-67 0.028775741976971858 1104 68-69 0.036806707890455925 1104 70-71 0.05915806157556602 1104 72-73 0.04851875995851529 1104 74-75 0.013210707547649747 1104 76-77 -0.04138371637197835 1104 78-79 -0.040003830940303686 1104 80-81 0.015640394366432275 1104 82-83 0.0907826237599032 1104 84-85 0.11785114233834548 1104 86-87 0.2497086677430751 1104 88-89 0.31101690695275863 1104 90-91 0.17826714126347198 1104 92-93 0.217945685785768 1104 94-95 0.3730284791649403 1104 96-97 0.4658308031040086 1104 98-99 0.7787901639156765 1104 100-101 1.0188467105133796 1105 1 0.14700007171002483 1105 2 0.153616711473191 1105 3 0.1814908300306186 1105 4 0.04300520450333778 1105 5 0.04959724290398526 1105 6 -0.005033802841779789 1105 7 0.11463265705580739 1105 8 0.08054532538513115 1105 9 0.2278006684488787 1105 10-11 0.167334887682955 1105 12-13 0.20256250178488244 1105 14-15 0.18722488560695894 1105 16-17 0.21742757099131893 1105 18-19 0.1660274875241825 1105 20-21 0.16939004802865298 1105 22-23 0.11127382099533634 1105 24-25 0.19379902182778608 1105 26-27 0.06966663076619994 1105 28-29 0.1428453251410957 1105 30-31 0.2005304448739409 1105 32-33 0.19066416861841162 1105 34-35 0.11621361152430154 1105 36-37 0.0805334083083693 1105 38-39 0.15509677434619817 1105 40-41 0.14683421384825124 1105 42-43 0.36370176880753746 1105 44-45 0.16696023444995944 1105 46-47 0.09680843586350818 1105 48-49 0.04601944353034071 1105 50-51 0.2483892793123772 1105 52-53 0.12675343888975732 1105 54-55 0.0904897281913648 1105 56-57 -0.010268216157278687 1105 58-59 0.0737791238069434 1105 60-61 0.2274190638294158 1105 62-63 0.15591960421163975 1105 64-65 0.08842125374406606 1105 66-67 0.1568853501527414 1105 68-69 0.10304964126732585 1105 70-71 0.12535714466607573 1105 72-73 0.09714573773100099 1105 74-75 0.02539889463928091 1105 76-77 -0.015053612612238254 1105 78-79 0.09489718999402186 1105 80-81 0.020537773733796172 1105 82-83 0.030987177372953312 1105 84-85 0.22604733888410067 1105 86-87 0.33411000469844154 1105 88-89 0.298884329997005 1105 90-91 0.15683890155946756 1105 92-93 0.2247966950698661 1105 94-95 0.41100561761883014 1105 96-97 0.584036026829164 1105 98-99 0.7541639427599485 1105 100-101 0.8592884742436553 1106 1 -0.014057953755965968 1106 2 0.06296058297904494 1106 3 0.04277917327971181 1106 4 0.05248943378292381 1106 5 0.026576804090645112 1106 6 -0.032416168160054326 1106 7 -0.0292913884735313 1106 8 0.02424094304753055 1106 9 -0.008706590089133215 1106 10-11 -0.05544149995648695 1106 12-13 -0.1642566811161572 1106 14-15 -0.028279104636034447 1106 16-17 0.1356776724168185 1106 18-19 0.024383310859860785 1106 20-21 0.048486126326622525 1106 22-23 -0.0034245609844418823 1106 24-25 0.12469644221822307 1106 26-27 0.10070472391638674 1106 28-29 0.09894572252746059 1106 30-31 0.16490962001373077 1106 32-33 0.2360023124183499 1106 34-35 0.17485267029564966 1106 36-37 0.16664554837382894 1106 38-39 0.12995490463953985 1106 40-41 0.26492405038310096 1106 42-43 -0.06585192119077021 1106 44-45 0.1434036894182995 1106 46-47 0.2818665066874857 1106 48-49 0.3768536777237088 1106 50-51 0.5351516135352341 1106 52-53 0.3567335569717187 1106 54-55 0.5132422398899408 1106 56-57 0.4003504038007435 1106 58-59 0.4102714520072652 1106 60-61 0.38869222475199194 1106 62-63 0.3226993508620737 1106 64-65 0.30446764992593245 1106 66-67 0.24853714947892058 1106 68-69 0.20994518938081086 1106 70-71 0.484045593246492 1106 72-73 0.5322315273253757 1106 74-75 0.37520001246169343 1106 76-77 0.3002990583432812 1106 78-79 0.3893206143709165 1106 80-81 0.33053505225452184 1106 82-83 0.30124991351797803 1106 84-85 0.2266395126152716 1106 86-87 0.3374853949661265 1106 88-89 0.12864036701920156 1106 90-91 0.3380594976138269 1106 92-93 0.5261261880935599 1106 94-95 0.8073805694259697 1106 96-97 0.7682760789404561 1106 98-99 0.7786780453143578 1106 100-101 0.9916811963025012 1107 1 0.30845135723100015 1107 2 0.3324335811168524 1107 3 0.2676087822182609 1107 4 0.16367006693003816 1107 5 0.14211032364372045 1107 6 0.18132591750102733 1107 7 0.22483337875179643 1107 8 0.1597092856918465 1107 9 0.2814759052553697 1107 10-11 0.3360259115891395 1107 12-13 0.3248285144145626 1107 14-15 0.4874722920120149 1107 16-17 0.4185077469047229 1107 18-19 0.5105411320889104 1107 20-21 0.5051164801441317 1107 22-23 0.5201089585686347 1107 24-25 0.4963365725720408 1107 26-27 0.4433467164675946 1107 28-29 0.5465025195479427 1107 30-31 0.4896396013917581 1107 32-33 0.5106810833680768 1107 34-35 0.5106129123813119 1107 36-37 0.4271511349660173 1107 38-39 0.5317849791274369 1107 40-41 0.5780846649082463 1107 42-43 0.5208477994796112 1107 44-45 0.5699125348559093 1107 46-47 0.5322868977489392 1107 48-49 0.6947051674816223 1107 50-51 0.6624509617661403 1107 52-53 0.6689086035266527 1107 54-55 0.8089224074877066 1107 56-57 0.6586441654394761 1107 58-59 0.63707592128101 1107 60-61 0.6469734165583247 1107 62-63 0.7144935780501598 1107 64-65 0.6495421378216477 1107 66-67 0.7244496262014621 1107 68-69 0.7030657666620073 1107 70-71 0.6553239917567382 1107 72-73 0.9196272442713784 1107 74-75 0.7218582992400933 1107 76-77 0.6964675872073407 1107 78-79 0.738146964464029 1107 80-81 0.823336262682826 1107 82-83 0.848174028974217 1107 84-85 0.8462866262093129 1107 86-87 0.9507572757854952 1107 88-89 0.9691021919726452 1107 90-91 1.13224338960638 1107 92-93 1.3733003035497937 1107 94-95 1.5158042193328605 1107 96-97 1.7052551292197826 1107 98-99 1.8190365183124975 1107 100-101 1.9883599672522223 1108 1 0.16273094169351054 1108 2 0.12536312041224562 1108 3 0.1023707515046155 1108 4 0.0756899404621123 1108 5 0.04919568949738107 1108 6 0.09977713701322699 1108 7 0.10210076809235602 1108 8 0.10495634982915902 1108 9 0.14718683569800817 1108 10-11 0.1377892358890449 1108 12-13 0.17202702389965907 1108 14-15 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74-75 -0.07164170075990484 2316 76-77 -0.0797824127926603 2316 78-79 -0.07235303553597561 2316 80-81 -0.08066373731717746 2316 82-83 -0.010075971025781172 2316 84-85 0.07803662620931107 2316 86-87 0.18425727578549456 2316 88-89 0.3333521919726472 2316 90-91 0.4989933896063832 2316 92-93 0.4770503035497953 2316 94-95 0.4210542193328628 2316 96-97 0.6197551292197829 2316 98-99 0.9230365183124967 2316 100-101 1.6418599672522234 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 8.0 4 22.0 5 44.0 6 117.0 7 224.0 8 588.0 9 1182.0 10 1847.0 11 3194.0 12 4744.0 13 6648.0 14 9062.0 15 12382.0 16 16837.0 17 22930.0 18 20472.0 19 13450.0 20 11954.0 21 12235.0 22 13026.0 23 14405.0 24 16863.0 25 20105.0 26 24594.0 27 30408.0 28 37949.0 29 47736.0 30 59525.0 31 75926.0 32 96299.0 33 126195.0 34 171640.0 35 243586.0 36 361631.0 37 485271.0 38 298395.0 39 18086.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.26093414132124 18.813844376473458 15.182111003708956 22.74311047849634 2 16.00605549879561 19.80328841133524 39.94497234360174 24.245683746267407 3 20.088340795962065 23.473085624068634 29.31139538362532 27.127178196343976 4 13.379324322012373 16.342724988013128 35.708905643459296 34.56904504651521 5 13.808288132068135 37.44332107977566 33.73403841749208 15.014352370664122 6 33.57283641160371 35.35224236383792 16.52312420572026 14.551797018838112 7 29.975069979965618 32.46329040292931 19.22252378836286 18.339115828742212 8 27.2600973599973 35.00638933207462 18.542179461927432 19.191333846000646 9 26.766015333519626 16.797779942893015 19.498671027702024 36.937533695885335 10-11 25.623744012605826 26.561613735155714 26.03553021366646 21.779112038572002 12-13 26.51502971154 23.525099676398543 27.106935441806023 22.852935170255424 14-15 23.228413467211737 25.03536834181369 25.615540750690585 26.120677440283984 16-17 22.475204873176256 28.451478583125585 26.30321098482572 22.77010555887244 18-19 22.49821348747862 26.611337785321076 28.95060539634257 21.939843330857734 20-21 23.039891980148983 25.466763409591497 29.47388138434212 22.019463225917395 22-23 22.9580438860912 25.3308916345936 29.27928431818326 22.431780161131936 24-25 22.702439614999424 25.634403866324558 29.442844978967624 22.220311539708394 26-27 22.189270137273546 26.07366074121354 29.370550837931237 22.36651828358168 28-29 22.213709331602583 26.411249240255586 29.21899699918778 22.156044428954054 30-31 22.324080609550613 26.030551228493305 29.12068051102374 22.524687650932343 32-33 22.27380821300055 25.831326020001043 29.223352888096542 22.67151287890187 34-35 22.828032870953038 25.920969686973176 29.08293234590041 22.168065096173375 36-37 22.88231916303917 25.667151112419344 28.972517317875525 22.47801240666596 38-39 22.377299658094614 25.996149287280364 29.128439562059295 22.498111492565727 40-41 22.83830156378348 25.92763758914794 28.831061155241073 22.402999691827507 42-43 22.490733802142106 25.838279400434878 28.763690895083123 22.907295902339893 44-45 22.62260534339542 26.072823486602033 28.879326700680235 22.425244469322315 46-47 22.91243434648736 26.589952276317447 28.130345006384943 22.36726837081025 48-49 22.96211452894194 26.538276113022523 27.955290906530628 22.544318451504903 50-51 23.10404850715987 26.776697121093747 27.838800200563174 22.280454171183216 52-53 23.21200694338126 26.681438387142197 27.643895961582416 22.462658707894125 54-55 22.882955244845434 26.752175070769585 27.771331661388647 22.59353802299633 56-57 22.93998326885511 26.997220103168086 27.682104737668894 22.38069189030791 58-59 23.112142099798163 26.46933800553698 27.650454184343527 22.768065710321327 60-61 22.784823175838014 26.76627853978428 27.907343498651727 22.541554785725975 62-63 22.62703102017432 26.75061776703701 28.25927220835759 22.36307900443108 64-65 22.69140688573909 26.64143103491387 28.088451342593224 22.578710736753816 66-67 22.653614852904987 26.79178761360092 27.72531443278392 22.829283100710175 68-69 22.775051758540133 26.52042013962687 28.068844396687016 22.635683705145983 70-71 22.789604756312674 26.689839053755932 27.80142491098145 22.71913127894995 72-73 22.802918606533392 26.62217310988291 27.719129085564408 22.855779198019285 74-75 22.701299054519232 27.188856197695983 27.289751932482332 22.820092815302463 76-77 22.95619238798709 26.73116243730756 27.586802136006572 22.725843038698777 78-79 22.959131524609127 26.777903483140104 27.554581302441107 22.708383689809665 80-81 22.96676775991702 27.020258350392407 27.38251830327405 22.630455586416524 82-83 22.821156281276863 27.06782353724358 27.41037064267996 22.700649538799595 84-85 22.81143627212395 27.13777567406857 27.420197173021332 22.630590880786148 86-87 22.825444676017213 27.093685199165986 27.3482933925138 22.732576732303 88-89 22.81270110603659 27.06960182594959 27.212040282841453 22.90565678517236 90-91 22.942388636720597 26.856456819555817 27.36462979228216 22.836524751441427 92-93 22.772373651807783 26.70425183392746 27.568178408393994 22.955196105870762 94-95 22.660918826749302 26.879281762744995 27.51336758816642 22.94643182233928 96-97 22.80706410520179 26.672006829325213 27.460857938366743 23.06007112710625 98-99 22.540747634565513 26.806371171145877 27.21798758921445 23.434893605074162 100-101 22.61460362589726 26.572223977254776 27.717996981344008 23.095175415503956 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 190.0 1 143.0 2 141.0 3 227.0 4 203.0 5 175.5 6 356.0 7 883.0 8 1610.5 9 2015.5 10 1980.5 11 1926.5 12 2082.0 13 2327.0 14 2560.0 15 2786.5 16 2966.5 17 3146.0 18 3386.5 19 3623.0 20 3966.5 21 4463.0 22 5090.0 23 5831.0 24 7056.5 25 8922.0 26 11387.0 27 14210.5 28 16563.0 29 18962.5 30 22389.0 31 26982.0 32 32705.5 33 36213.0 34 39246.0 35 43417.0 36 48497.0 37 54444.5 38 57783.0 39 60499.0 40 63851.0 41 67639.5 42 70034.0 43 72549.0 44 77274.5 45 81611.0 46 84584.0 47 89683.5 48 93923.0 49 91985.0 50 88590.0 51 85115.0 52 83127.5 53 84131.5 54 86183.0 55 80498.5 56 69778.0 57 63542.0 58 58950.0 59 51873.5 60 42095.0 61 34515.5 62 28872.0 63 21783.0 64 15178.0 65 10937.0 66 7877.5 67 5927.5 68 4435.0 69 3292.0 70 2525.5 71 1711.5 72 1114.0 73 870.5 74 680.5 75 487.0 76 358.5 77 249.0 78 167.5 79 103.0 80 71.5 81 55.0 82 34.0 83 22.0 84 15.0 85 8.0 86 5.5 87 3.0 88 1.0 89 0.0 90 0.5 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.022109326231443408 2 0.0 3 0.0 4 1.7547084310669373E-4 5 0.022591871049986816 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.003487483006745538 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 8.992880709218055E-4 24-25 0.0 26-27 3.728755416017242E-4 28-29 0.001381832889465213 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 1.3160313233002028E-4 40-41 5.922140954850914E-4 42-43 0.01443247684552556 44-45 2.1933855388336716E-5 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 2.4127240927170388E-4 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 1.0966927694168358E-4 82-83 0.001776642286455274 84-85 4.167432523783976E-4 86-87 0.0 88-89 0.0 90-91 0.01048438287562495 92-93 0.0020617824065036513 94-95 0.0 96-97 0.0 98-99 0.001381832889465213 100-101 0.018468306236979513 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2279581.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 47.43752235149678 #Duplication Level Percentage of deduplicated Percentage of total 1 72.52752352848371 34.40526018481155 2 14.57189185120574 13.825088907903323 3 5.1563983466214065 7.33820285463222 4 2.38533494128732 4.5261751837249395 5 1.341197071988177 3.181153304010059 6 0.8517215407966514 2.4242135777275458 7 0.5644984842595667 1.8744886625114476 8 0.39072236831879165 1.482792086428195 9 0.2870466964206192 1.2255105669638788 >10 1.7033595468830445 15.190063813237492 >50 0.14109170314122024 4.605761796263338 >100 0.07309546195696422 6.434045150437613 >500 0.0041716763431971365 1.3378953001962093 >1k 0.0016686705372788125 1.1774252627108641 >5k 2.7811175621313545E-4 0.9719233484415033 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9341 0.40976828636490653 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7680 0.336904018764852 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5080 0.22284797074550103 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2294 0.10063252852168884 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 4.386771077667343E-5 0.0 0.0 0.0 0.0 22-23 4.386771077667343E-5 0.0 0.0 0.0 6.580156616501015E-5 24-25 4.386771077667343E-5 0.0 0.0 1.316031323300203E-4 1.7547084310669373E-4 26-27 4.386771077667343E-5 0.0 0.0 3.0707397543671403E-4 1.7547084310669373E-4 28-29 4.386771077667343E-5 0.0 0.0 9.650896370868155E-4 1.7547084310669373E-4 30-31 4.386771077667343E-5 0.0 0.0 0.0024127240927170387 1.9740469849503046E-4 32-33 4.386771077667343E-5 0.0 0.0 0.004693845053104058 2.1933855388336717E-4 34-35 4.386771077667343E-5 0.0 0.0 0.007303973844316127 2.1933855388336717E-4 36-37 4.386771077667343E-5 0.0 0.0 0.009299954684654766 2.1933855388336717E-4 38-39 4.386771077667343E-5 0.0 0.0 0.010835324561838337 2.1933855388336717E-4 40-41 4.386771077667343E-5 0.0 0.0 0.013511254919215416 2.1933855388336717E-4 42-43 4.386771077667343E-5 0.0 0.0 0.016932936359795946 2.1933855388336717E-4 44-45 4.386771077667343E-5 0.0 0.0 0.020091411535716433 2.4127240927170388E-4 46-47 4.386771077667343E-5 0.0 0.0 0.024719455022655476 3.5094168621338745E-4 48-49 4.386771077667343E-5 0.0 0.0 0.030224852725127996 3.9480939699006087E-4 50-51 4.386771077667343E-5 0.0 0.0 0.03875712247119098 3.9480939699006087E-4 52-53 4.386771077667343E-5 0.0 0.0 0.05713769328661715 4.167432523783976E-4 54-55 4.386771077667343E-5 0.0 0.0 0.08038757999825406 4.3867710776673434E-4 56-57 4.386771077667343E-5 0.0 0.0 0.10855065031687841 4.3867710776673434E-4 58-59 4.386771077667343E-5 0.0 0.0 0.13936771713749152 4.8254481854340776E-4 60-61 4.386771077667343E-5 0.0 0.0 0.18575782128382365 5.483463847084179E-4 62-63 4.386771077667343E-5 0.0 0.0 0.220435246652784 6.141479508734281E-4 64-65 4.386771077667343E-5 0.0 0.0 0.25535394443101606 6.141479508734281E-4 66-67 4.386771077667343E-5 0.0 0.0 0.3158475175920487 6.141479508734281E-4 68-69 4.386771077667343E-5 0.0 0.0 0.3923528051865672 6.141479508734281E-4 70-71 4.386771077667343E-5 0.0 0.0 0.4621463330322546 6.799495170384382E-4 72-73 4.386771077667343E-5 0.0 0.0 0.5382348773743947 7.238172278151117E-4 74-75 4.386771077667343E-5 0.0 0.0 0.6424426243243824 7.457510832034484E-4 76-77 4.386771077667343E-5 0.0 0.0 0.7376794244205405 7.457510832034484E-4 78-79 4.386771077667343E-5 0.0 0.0 0.830152558737768 7.457510832034484E-4 80-81 4.386771077667343E-5 0.0 0.0 0.9395805632701799 7.457510832034484E-4 82-83 4.386771077667343E-5 0.0 0.0 1.093709765084022 7.896187939801217E-4 84-85 4.386771077667343E-5 0.0 0.0 1.2578188710995573 7.896187939801217E-4 86-87 4.386771077667343E-5 0.0 0.0 1.4461429534638164 7.896187939801217E-4 88-89 4.386771077667343E-5 0.0 0.0 1.6784005481709139 7.896187939801217E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 8390 0.0 33.30045 1 GTATCAA 12245 0.0 32.90575 1 GGACCGA 615 0.0 28.575865 6 TATCAAC 15335 0.0 26.147291 2 ATCAACG 15300 0.0 26.113953 3 TCAACGC 15465 0.0 25.958212 4 CAACGCA 15700 0.0 25.569666 5 AGGACCG 680 0.0 25.151365 5 AACGCAG 16065 0.0 25.101501 6 TACATGG 10385 0.0 23.239418 2 GTACATG 10535 0.0 23.227764 1 ACATGGG 10420 0.0 22.523052 3 GAGTACT 10125 0.0 22.33175 12-13 CGCAGAG 18290 0.0 21.71218 8 ACGCAGA 18465 0.0 21.686483 7 GTATAGG 595 0.0 21.561626 1 GTACTTT 10345 0.0 21.48761 14-15 AGTACTT 10410 0.0 20.990267 12-13 TAAGGTG 1115 0.0 20.451935 5 GCAGAGT 19340 0.0 20.312338 9 >>END_MODULE