FastQCFastQC Report
Thu 26 May 2016
SRR937613_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937613_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2200200
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT75600.34360512680665395No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59380.26988455594945915No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37250.16930279065539497No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN31590.1435778565584947No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA102500.035.478421
GGTATCA67950.034.6292041
ATCAACG125600.028.8286193
TCAACGC126250.028.643864
TATCAAC127250.028.5713922
CAACGCA129300.028.0055835
AACGCAG132050.027.4136096
CGCAGAG148550.024.3692328
ACGCAGA148700.024.344657
GTACATG102250.024.1750161
TACATGG100650.024.0815332
TAAGGTG9500.023.0103055
GAGTACT77200.022.94995312-13
ACATGGG102100.022.9445573
GCAGAGT157500.022.4108289
GTACTTT78850.022.28898614-15
GTATAGG6200.022.234791
GTAAGGT9350.021.8542124
AGTACTT79600.021.7209412-13
AGAGTAC137300.019.9272410-11
Produced by FastQC (version 0.11.5)