##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937613_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2200200 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.672792018907373 33.0 31.0 34.0 30.0 34.0 2 31.801624852286157 33.0 31.0 34.0 30.0 34.0 3 31.823635578583765 33.0 31.0 34.0 30.0 34.0 4 35.43111080810835 37.0 35.0 37.0 33.0 37.0 5 35.4213589673666 37.0 35.0 37.0 33.0 37.0 6 35.472169802745206 37.0 35.0 37.0 33.0 37.0 7 35.41324243250614 37.0 35.0 37.0 33.0 37.0 8 35.38553495136806 37.0 35.0 37.0 33.0 37.0 9 37.003147441141714 39.0 37.0 39.0 33.0 39.0 10-11 36.88142850649941 39.0 37.0 39.0 33.0 39.0 12-13 36.74806313062449 39.0 37.0 39.0 33.0 39.0 14-15 37.874519361876196 40.0 38.0 41.0 33.0 41.0 16-17 37.79195845832197 40.0 38.0 41.0 33.0 41.0 18-19 37.714708662848835 40.0 37.5 41.0 32.0 41.0 20-21 37.613055176802106 40.0 37.0 41.0 32.0 41.0 22-23 37.41310312698846 40.0 37.0 41.0 32.0 41.0 24-25 37.22441368966457 39.5 37.0 41.0 31.5 41.0 26-27 37.16300199981819 39.0 36.5 41.0 31.5 41.0 28-29 37.21399759112808 39.0 37.0 41.0 31.0 41.0 30-31 37.040415871284424 39.0 36.5 41.0 31.0 41.0 32-33 36.79551768021089 39.0 36.0 41.0 30.5 41.0 34-35 36.82084765021362 39.0 36.0 41.0 30.0 41.0 36-37 36.727434551404414 39.0 36.0 41.0 30.0 41.0 38-39 36.57960389964549 39.0 36.0 41.0 30.0 41.0 40-41 36.33937255704027 39.0 35.0 41.0 30.0 41.0 42-43 36.071597582037995 39.0 35.0 40.5 29.5 41.0 44-45 35.83126124897737 39.0 35.0 40.0 28.5 41.0 46-47 35.482618398327425 39.0 35.0 40.0 27.0 41.0 48-49 35.11323675120444 38.0 34.5 40.0 26.0 41.0 50-51 34.166721888919184 37.5 33.5 39.5 23.5 40.5 52-53 34.261808017452964 38.0 33.5 39.5 24.0 40.5 54-55 34.59657190255432 38.0 34.0 40.0 24.0 41.0 56-57 34.356473047904736 38.0 34.0 40.0 23.5 41.0 58-59 34.08390259976366 38.0 34.0 40.0 23.0 41.0 60-61 33.723889646395776 37.5 33.0 40.0 22.0 41.0 62-63 33.33084946823016 37.0 33.0 40.0 20.5 41.0 64-65 32.92956913007908 36.0 32.0 39.0 19.5 41.0 66-67 32.48503931460776 36.0 31.5 39.0 18.0 40.5 68-69 31.96991068993728 35.0 31.0 38.5 15.0 40.0 70-71 31.495078401963458 35.0 31.0 38.0 11.5 40.0 72-73 31.015410871738933 35.0 30.5 37.0 7.5 39.0 74-75 30.54382306153986 34.5 30.0 37.0 7.0 39.0 76-77 30.031063994182347 34.0 29.5 36.0 2.0 39.0 78-79 29.481342150713573 34.0 29.0 35.5 2.0 37.5 80-81 28.945164757749296 34.0 28.5 35.0 2.0 37.0 82-83 28.39805426779384 34.0 27.5 35.0 2.0 36.5 84-85 27.91273020634488 33.0 26.5 35.0 2.0 36.0 86-87 27.422784065084993 33.0 26.0 35.0 2.0 36.0 88-89 26.907090491773474 33.0 25.0 35.0 2.0 35.0 90-91 26.366158985546768 32.0 24.5 35.0 2.0 35.0 92-93 25.754145532224342 32.0 23.5 34.0 2.0 35.0 94-95 25.06219502772475 32.0 19.0 34.0 2.0 35.0 96-97 24.27501340787201 31.0 12.0 34.0 2.0 35.0 98-99 23.26367466593946 31.0 2.0 34.0 2.0 35.0 100-101 20.856734842287068 28.0 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.12409140366927929 1101 2 0.20922860600201432 1101 3 0.15242071004317026 1101 4 0.08980726443416387 1101 5 0.1337544633178851 1101 6 0.11683270091746323 1101 7 0.1763146193079308 1101 8 0.1738501024586938 1101 9 0.206984595195415 1101 10-11 0.26338350925701803 1101 12-13 0.3012304937252992 1101 14-15 0.5105005367688094 1101 16-17 0.6241649950328423 1101 18-19 0.5007961711834454 1101 20-21 0.4687919399857208 1101 22-23 0.5104663705787473 1101 24-25 0.6246876878436254 1101 26-27 0.5770549655544528 1101 28-29 0.27298209035237164 1101 30-31 0.4755434059222665 1101 32-33 0.45680945244470195 1101 34-35 0.4344235264013818 1101 36-37 0.484153277691739 1101 38-39 0.38559200025800777 1101 40-41 0.42373651421464587 1101 42-43 0.3970622791135838 1101 44-45 0.40827243626910814 1101 46-47 0.39061693438796397 1101 48-49 0.42945857398353127 1101 50-51 0.43845797515832174 1101 52-53 0.39389810941671044 1101 54-55 0.5422765302348935 1101 56-57 0.4974852142306432 1101 58-59 0.5845628702556596 1101 60-61 0.4779796581913658 1101 62-63 0.6292340676209065 1101 64-65 0.4583413860416883 1101 66-67 0.6986218962130692 1101 68-69 0.6081013009328728 1101 70-71 0.6936226798625569 1101 72-73 0.7777709111434241 1101 74-75 0.7814022972539227 1101 76-77 0.7934432823436524 1101 78-79 0.8800574004070718 1101 80-81 0.8769855927395227 1101 82-83 0.8557327887356969 1101 84-85 1.1244528867560675 1101 86-87 1.2474431436827587 1101 88-89 1.2004120852189004 1101 90-91 1.2176311867024232 1101 92-93 1.4291229178324478 1101 94-95 1.4778021220451727 1101 96-97 1.7433199185600508 1101 98-99 1.8818469474939512 1101 100-101 2.3503923523153425 1102 1 0.24249051292560964 1102 2 0.10248501338016425 1102 3 0.18331033818776277 1102 4 0.16831209355034815 1102 5 0.0783845959258116 1102 6 0.07241423882810238 1102 7 0.029761372789934626 1102 8 0.10635350411897093 1102 9 0.176848385409059 1102 10-11 0.11922995878393294 1102 12-13 0.19432306889142126 1102 14-15 0.1954189336400134 1102 16-17 0.24175062577636908 1102 18-19 0.2355929090865274 1102 20-21 0.27636165260268797 1102 22-23 0.47550655230835304 1102 24-25 0.4491168191342467 1102 26-27 0.3064638617309967 1102 28-29 0.12848452720353976 1102 30-31 0.3039256668627459 1102 32-33 0.2140721851100551 1102 34-35 0.24696485600556173 1102 36-37 0.3524695091801817 1102 38-39 0.4390314625946061 1102 40-41 0.5696695762544266 1102 42-43 0.5369631859782089 1102 44-45 0.5705115242919376 1102 46-47 0.6284435191280338 1102 48-49 0.5979233734875251 1102 50-51 0.49866307840058965 1102 52-53 0.4636315332511671 1102 54-55 0.6684914151519408 1102 56-57 0.6750368441076304 1102 58-59 0.6332145678686132 1102 60-61 0.6882594388485259 1102 62-63 0.9153832867689644 1102 64-65 0.7916438587715859 1102 66-67 0.906776404151671 1102 68-69 0.819161776175676 1102 70-71 0.8117896789145362 1102 72-73 0.7875401717443502 1102 74-75 0.7376188816897589 1102 76-77 0.8133022992174972 1102 78-79 0.9316030882628219 1102 80-81 0.8675248507492057 1102 82-83 0.7496613977565012 1102 84-85 0.8921639007218474 1102 86-87 0.959079566958053 1102 88-89 1.1488479509401301 1102 90-91 1.0831631448243222 1102 92-93 1.0288345766362177 1102 94-95 1.4750625222433804 1102 96-97 1.7208858334869 1102 98-99 1.8701327694857923 1102 100-101 2.2567430098997256 1103 1 0.22973916127467575 1103 2 0.19563771821344034 1103 3 0.2608650933591825 1103 4 0.29405008693757395 1103 5 0.24409382798972246 1103 6 0.19284845167647546 1103 7 0.3054662585686856 1103 8 0.21083280311276553 1103 9 0.1576335753694309 1103 10-11 0.26364330508805267 1103 12-13 0.3231921560110962 1103 14-15 0.37404277651451423 1103 16-17 0.32080114842445795 1103 18-19 0.3358207244365019 1103 20-21 0.318995446720038 1103 22-23 0.4818633589847181 1103 24-25 0.5230323808770194 1103 26-27 0.37724395428644897 1103 28-29 0.20350835520149246 1103 30-31 0.26587543518240864 1103 32-33 0.1591496525384386 1103 34-35 0.27164488263749575 1103 36-37 0.41067180979435136 1103 38-39 0.4167203801508279 1103 40-41 0.4212226074966381 1103 42-43 0.542130233552534 1103 44-45 0.4587288618859944 1103 46-47 0.455811679380524 1103 48-49 0.5192944441467446 1103 50-51 0.49375755540975774 1103 52-53 0.4893361455639891 1103 54-55 0.490811485509262 1103 56-57 0.5875324868838234 1103 58-59 0.49845074033736836 1103 60-61 0.67150080862843 1103 62-63 0.7315274025843408 1103 64-65 0.6750174766747747 1103 66-67 0.6234084561087485 1103 68-69 0.8595734773087713 1103 70-71 0.8716273969398003 1103 72-73 0.9083195897505156 1103 74-75 0.9196722607806258 1103 76-77 0.8373398632267559 1103 78-79 1.048914502511927 1103 80-81 0.9498978021247737 1103 82-83 1.0009726668863586 1103 84-85 1.1343429574612856 1103 86-87 1.1099207299247844 1103 88-89 1.2329209488413753 1103 90-91 1.301914557731621 1103 92-93 1.2372407734470947 1103 94-95 1.187784649502028 1103 96-97 1.4935975435167528 1103 98-99 1.8148132555404892 1103 100-101 2.254410527835443 1104 1 0.2013066095368572 1104 2 0.16460897566877009 1104 3 0.1773421476823458 1104 4 0.1402532935044647 1104 5 0.11661233456777609 1104 6 0.16942228579306118 1104 7 0.16395758496644675 1104 8 0.17621589237833035 1104 9 0.2743040652822586 1104 10-11 0.3069872918451608 1104 12-13 0.3485464779325582 1104 14-15 0.5265972732719888 1104 16-17 0.5440193090503911 1104 18-19 0.5213204950417278 1104 20-21 0.5045099419895465 1104 22-23 0.5048369351871784 1104 24-25 0.44145231590250233 1104 26-27 0.4597904222269804 1104 28-29 0.41778786962855463 1104 30-31 0.5817055342561446 1104 32-33 0.4292914433056083 1104 34-35 0.5060018642983195 1104 36-37 0.5295261999142582 1104 38-39 0.6612916289263566 1104 40-41 0.7603091513234332 1104 42-43 0.7631296197665307 1104 44-45 0.7671710544040096 1104 46-47 0.8969323968281699 1104 48-49 0.7372442748906067 1104 50-51 0.690964984080388 1104 52-53 0.6847432902810908 1104 54-55 0.7479699972951934 1104 56-57 0.9001936832085491 1104 58-59 0.7637372668045685 1104 60-61 0.8877061541465494 1104 62-63 0.8933026391089456 1104 64-65 0.7654501648094865 1104 66-67 0.7846133056381746 1104 68-69 0.8438777601711251 1104 70-71 0.7522917427927212 1104 72-73 0.8147334985133945 1104 74-75 0.9372837342390312 1104 76-77 0.9405256605905734 1104 78-79 0.9787964262040667 1104 80-81 0.9592053399129128 1104 82-83 1.204036637027837 1104 84-85 1.2994079939784555 1104 86-87 1.5150156187613213 1104 88-89 1.3928711019778817 1104 90-91 1.360856613852743 1104 92-93 1.587159245305937 1104 94-95 1.5321565728221742 1104 96-97 1.6693542362641587 1104 98-99 1.8462744382255245 1104 100-101 2.4351495819850086 1105 1 0.25096582480320606 1105 2 0.16833180408540116 1105 3 0.2500403693315256 1105 4 0.1475121470153553 1105 5 0.1808800319236994 1105 6 0.19703576723544103 1105 7 0.2810807465611944 1105 8 0.21229351850944767 1105 9 0.15391636328671865 1105 10-11 0.3433037278478963 1105 12-13 0.3432419149724808 1105 14-15 0.42833746494186187 1105 16-17 0.4694313059842088 1105 18-19 0.45001163623562235 1105 20-21 0.49204938291599376 1105 22-23 0.6289109034192748 1105 24-25 0.5448531029559618 1105 26-27 0.5362448825607942 1105 28-29 0.5588398223851812 1105 30-31 0.3932520808531734 1105 32-33 0.4610498992553289 1105 34-35 0.4950435065201546 1105 36-37 0.43326722536993856 1105 38-39 0.5152778311160517 1105 40-41 0.6683412949430405 1105 42-43 0.7131553183563142 1105 44-45 0.6287923433427309 1105 46-47 0.6163567484168198 1105 48-49 0.4355602713066773 1105 50-51 0.34718803523212927 1105 52-53 0.4576016921019672 1105 54-55 0.5204133973594054 1105 56-57 0.5284558071434091 1105 58-59 0.5507326246684627 1105 60-61 0.5398857223598128 1105 62-63 0.5786534553280873 1105 64-65 0.5079314886798088 1105 66-67 0.6640323914323787 1105 68-69 0.6649650395582896 1105 70-71 0.7715032772780006 1105 72-73 0.8340616537454828 1105 74-75 0.838860341672163 1105 76-77 1.0763685533230216 1105 78-79 1.092859816619228 1105 80-81 1.1086649130683703 1105 82-83 1.0494917689290553 1105 84-85 1.1513368380924476 1105 86-87 1.3219389407253566 1105 88-89 1.1616099547155088 1105 90-91 1.359187151089852 1105 92-93 1.4460798709142573 1105 94-95 1.5878693504215775 1105 96-97 1.7613810334003723 1105 98-99 2.094185876220269 1105 100-101 2.2213197692674207 1106 1 0.1430813891815852 1106 2 0.20709775143944853 1106 3 0.24609899859154538 1106 4 0.1556218536498193 1106 5 0.22397077413712196 1106 6 0.1428219884823676 1106 7 0.23394947649308762 1106 8 0.15589778495607476 1106 9 0.11946225559113088 1106 10-11 0.12570941896800036 1106 12-13 0.12046938737292123 1106 14-15 0.1694124526344183 1106 16-17 0.18610716586075426 1106 18-19 0.30331142556412516 1106 20-21 0.44468367353901783 1106 22-23 0.3125797764563103 1106 24-25 0.23043398925114644 1106 26-27 0.35388099687748564 1106 28-29 0.490078100419737 1106 30-31 0.47344427624152274 1106 32-33 0.5504943503434632 1106 34-35 0.41736611494023634 1106 36-37 0.48119162778638014 1106 38-39 0.5606029717796375 1106 40-41 0.7604743239288112 1106 42-43 0.7198140424882666 1106 44-45 0.7582104405625429 1106 46-47 0.762243521953927 1106 48-49 0.8884541642249602 1106 50-51 0.8447697725843639 1106 52-53 0.8841817034228185 1106 54-55 0.8669205506234192 1106 56-57 0.8845717332708602 1106 58-59 0.800332383701722 1106 60-61 0.8917434981710599 1106 62-63 1.0858291418042612 1106 64-65 1.057896708948114 1106 66-67 1.0078515896449574 1106 68-69 0.9726889303439492 1106 70-71 1.1539474104654346 1106 72-73 1.157961233996975 1106 74-75 1.2935760597191575 1106 76-77 1.3515877204431206 1106 78-79 1.3719391353351078 1106 80-81 1.4209040650807125 1106 82-83 1.3837425547118194 1106 84-85 1.4855481354262707 1106 86-87 1.7391107356584783 1106 88-89 1.8215634604925306 1106 90-91 2.0079700348777116 1106 92-93 1.984677151517932 1106 94-95 2.050865185822758 1106 96-97 2.5015135302263474 1106 98-99 2.5755584149812343 1106 100-101 3.016723263982694 1107 1 0.1909599639588926 1107 2 0.17057641998140838 1107 3 0.27900453070593656 1107 4 0.2158480608696962 1107 5 0.09086599213993907 1107 6 0.06127018351284619 1107 7 0.10362247227368471 1107 8 0.22465071510141144 1107 9 0.16671377926670772 1107 10-11 0.17629251788970635 1107 12-13 0.2429406719486309 1107 14-15 0.3620460672617085 1107 16-17 0.4820284035541462 1107 18-19 0.39477591220266817 1107 20-21 0.35064065900549224 1107 22-23 0.46264716979901976 1107 24-25 0.549254312739194 1107 26-27 0.4256695575900977 1107 28-29 0.3926214665706951 1107 30-31 0.3376935730066464 1107 32-33 0.2679257849426193 1107 34-35 0.5357410563373293 1107 36-37 0.40219490954915926 1107 38-39 0.5540687476821233 1107 40-41 0.4101139160525804 1107 42-43 0.41560342364157066 1107 44-45 0.5170565493295385 1107 46-47 0.45892659743446984 1107 48-49 0.48790329690194056 1107 50-51 0.3483855719280129 1107 52-53 0.30281449760941115 1107 54-55 0.3555545168681462 1107 56-57 0.666094236787039 1107 58-59 0.8193373063458935 1107 60-61 0.7641708305667478 1107 62-63 0.7939427142374527 1107 64-65 0.732114360143612 1107 66-67 0.6954072624063272 1107 68-69 0.79210993362571 1107 70-71 0.9760231254208946 1107 72-73 1.027842270096368 1107 74-75 0.9999608464045764 1107 76-77 1.0673050200211804 1107 78-79 1.1452777429300447 1107 80-81 1.144550337935847 1107 82-83 1.0901156208158973 1107 84-85 1.1537970805739448 1107 86-87 1.2111249175619 1107 88-89 1.2430795176889688 1107 90-91 1.332202336799888 1107 92-93 1.5151330821320848 1107 94-95 1.6997458703047101 1107 96-97 1.7928813220688262 1107 98-99 2.0742515701617315 1107 100-101 2.467112973312279 1108 1 0.2846712091407255 1108 2 0.21538955891219658 1108 3 0.3490124911550758 1108 4 0.3105166548029956 1108 5 0.2636852480474303 1108 6 0.33680646640206646 1108 7 0.30307571510928 1108 8 0.2952180018451642 1108 9 0.31737784831312865 1108 10-11 0.4159680248446307 1108 12-13 0.46033111103025703 1108 14-15 0.559431688176744 1108 16-17 0.544013069215282 1108 18-19 0.6047338056164406 1108 20-21 0.7499923013718401 1108 22-23 0.6814621103051337 1108 24-25 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-0.6924748356599721 2316 90-91 -0.7196236562681442 2316 92-93 -0.560486394522421 2316 94-95 -0.41155124258475695 2316 96-97 -0.3187619355935425 2316 98-99 -0.04399974092397585 2316 100-101 -0.09523062641189384 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 9175.0 3 1855.0 4 1824.0 5 2021.0 6 2347.0 7 2822.0 8 3444.0 9 4255.0 10 5283.0 11 6789.0 12 8812.0 13 10829.0 14 12371.0 15 15182.0 16 18760.0 17 23596.0 18 20855.0 19 16291.0 20 16026.0 21 17300.0 22 18685.0 23 21229.0 24 23627.0 25 26766.0 26 30891.0 27 36251.0 28 43322.0 29 51571.0 30 62096.0 31 76411.0 32 97036.0 33 124429.0 34 166535.0 35 227561.0 36 317833.0 37 394436.0 38 256927.0 39 24754.0 40 3.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.27892426726284 18.054428516983382 14.410163223551164 23.256483992202615 2 15.42426564192834 19.103132145938613 40.980022251686535 24.492579960446513 3 19.798476335621807 23.38965191615653 28.83851126432423 27.97336048389743 4 12.766286122841242 15.730709307989871 36.026440755510045 35.47656381365884 5 13.865591402749159 37.38108105398708 33.628487142309034 15.124840400954723 6 33.473440262796146 34.92347410526968 16.940868134601036 14.662217497333142 7 30.05788218340941 32.214493363520056 19.297507317821875 18.43011713524865 8 27.101347414968373 34.89928208622709 18.685572351870217 19.313798146934325 9 26.69910474901178 17.019835453105227 19.59995663585322 36.68110316202977 10-11 25.487681419355575 26.412039014058042 26.21152543144925 21.888754135137138 12-13 26.411862944195963 23.488692365521924 27.088082283221848 23.011362407060265 14-15 23.267950308324053 24.982344556372958 25.499789683299717 26.24991545200327 16-17 22.534919057383583 28.43490293353787 26.130513876983258 22.89966413209529 18-19 22.554100595463527 26.83691969520612 28.478993439584716 22.12998626974564 20-21 23.161055549344216 25.750789744715437 28.870056510039944 22.21809819590041 22-23 22.98823958540389 25.550002197208205 28.73158244300863 22.730175774379276 24-25 22.67958515830708 25.83819006321718 28.8749782551475 22.607246523328243 26-27 22.261094316635642 26.241920149385432 28.78525274262324 22.711732791355686 28-29 22.32007750885731 26.598506305566005 28.660170851094264 22.421245334482425 30-31 22.461707408779745 26.15891211851169 28.595376945488184 22.78400352722038 32-33 22.206259447372684 26.124569010772824 28.666696577890306 23.002474963964186 34-35 22.774335184818277 26.22149043399445 28.504077593843867 22.500096787343406 36-37 22.83940216017659 25.88120076198665 28.50903458769063 22.770362490146134 38-39 22.37407830937655 26.286313733901867 28.535395971179906 22.804211985541677 40-41 22.809284042727107 26.205462428443205 28.364700455331395 22.620553073498293 42-43 22.442707484120962 26.116109856617275 28.31039112577437 23.130791533487393 44-45 22.590494025662565 26.362297630462983 28.39921731204115 22.647991031833303 46-47 22.926638100896206 26.66574519020026 27.760085783842115 22.64753092506142 48-49 22.86471922619792 26.62532847714364 27.653795851013996 22.856156445644448 50-51 22.946368420405335 26.940279233494902 27.533915741650482 22.57943660444928 52-53 23.046190507970994 26.76932033872477 27.408102216033736 22.776386937270498 54-55 22.689121749100778 26.84699353378339 27.557332307058186 22.90655241005765 56-57 22.89936159333482 27.12167427281697 27.32317692574931 22.6557872080989 58-59 23.018792151663117 26.591040307143675 27.45174015907216 22.938427382121045 60-61 22.62944645988504 26.867115856693296 27.735814518549013 22.767623164872656 62-63 22.613036950275898 26.871615563025042 27.959690276911896 22.55565720978716 64-65 22.55131326533911 26.875050941025886 27.765511457006685 22.808124336628318 66-67 22.488291996436313 26.99442473852372 27.464839560108295 23.052443704931672 68-69 22.650082617418168 26.776650179613476 27.737981311637185 22.835285891331168 70-71 22.661611051561266 26.81072771532886 27.5834818537903 22.944179379319575 72-73 22.626346384853644 26.870207908141193 27.529750927340068 22.97369477966509 74-75 22.56365232660228 27.40139763714986 27.19691146415834 22.838038572089516 76-77 22.831270674599395 27.018567344483063 27.262529325309515 22.88763265560803 78-79 22.793679929129215 27.012860132677762 27.24646955539513 22.946990382797892 80-81 22.86864934950193 27.19472223752937 27.062491261643363 22.87413715132534 82-83 22.813830378303347 27.243415168202628 27.094426460649494 22.848327992844535 84-85 22.7800560523082 27.30096683287154 27.12138906262772 22.797588052192534 86-87 22.821857553713414 27.252814459798813 27.050008048957704 22.87531993753007 88-89 22.783257372974656 27.223790260603042 26.913056794362806 23.079895572059492 90-91 22.891151722492133 27.07801629677398 27.065469456755984 22.965362523977902 92-93 22.81875603402736 26.83509572472084 27.264832322349125 23.08131591890267 94-95 22.660292572297735 27.08444384736329 27.201732344961616 23.05353123537736 96-97 22.86274950867805 26.856778057261092 27.102347963404483 23.17812447065638 98-99 22.561358106014257 27.063984657143486 26.9531665361607 23.421490700681552 100-101 22.999312209678376 26.308696961769922 27.48383715511163 23.20815367344007 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 2866.0 1 1654.0 2 364.5 3 300.5 4 267.0 5 250.0 6 390.0 7 774.5 8 1319.5 9 1677.0 10 1703.0 11 1681.0 12 1808.5 13 2026.0 14 2262.5 15 2436.0 16 2565.0 17 2695.0 18 2881.0 19 3145.5 20 3393.5 21 3755.0 22 4389.5 23 5295.5 24 6494.0 25 8280.5 26 10724.5 27 13424.0 28 15749.0 29 18208.0 30 21423.5 31 25621.0 32 31086.0 33 35025.5 34 38190.5 35 41995.5 36 46867.5 37 52478.0 38 55808.5 39 58324.0 40 61219.0 41 65153.0 42 68019.0 43 70195.0 44 74047.5 45 78530.0 46 81806.0 47 85721.5 48 89724.5 49 88930.5 50 85795.0 51 82076.0 52 79853.0 53 80722.5 54 82358.0 55 77303.5 56 67899.0 57 61379.5 58 56741.0 59 50347.0 60 41289.0 61 34214.5 62 28365.5 63 21491.0 64 15463.0 65 11583.0 66 8468.5 67 6100.0 68 4578.0 69 3417.0 70 2542.5 71 1845.0 72 1348.0 73 1049.0 74 765.5 75 513.0 76 358.0 77 248.5 78 172.0 79 111.0 80 70.5 81 44.0 82 40.5 83 34.0 84 20.5 85 17.5 86 13.0 87 9.0 88 6.5 89 4.5 90 5.0 91 5.5 92 4.0 93 2.5 94 4.0 95 4.5 96 3.5 97 1.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.30197254795018635 2 0.2822016180347241 3 0.2734296882101627 4 0.25947641123534226 5 0.25624943186983 6 0.2151622579765476 7 0.2060267248431961 8 0.2186619398236524 9 0.21988910099081904 10-11 0.21986637578401963 12-13 0.21627579310971728 14-15 0.21368511953458774 16-17 0.21334424143259703 18-19 0.21286701208981001 20-21 0.19977729297336605 22-23 0.1923234251431688 24-25 0.19443686937551133 26-27 0.20623125170439052 28-29 0.21988910099081904 30-31 0.21377602036178528 32-33 0.21882101627124806 34-35 0.2120261794382329 36-37 0.1982092537042087 38-39 0.19866375784019633 40-41 0.19166439414598674 42-43 0.2062539769111899 44-45 0.20150440869011907 46-47 0.20218616489410055 48-49 0.21141259885464955 50-51 0.2196618489228252 52-53 0.23109262794291427 54-55 0.2071402599763658 56-57 0.17957458412871558 58-59 0.1801654395054995 60-61 0.1863012453413326 62-63 0.19557312971548044 64-65 0.1833015180438142 66-67 0.1890509953640578 68-69 0.19245977638396508 70-71 0.1860739932733388 72-73 0.18789200981728932 74-75 0.19125534042359785 76-77 0.20775384055994908 78-79 0.21157167530224527 80-81 0.20093627852013451 82-83 0.19980001818016543 84-85 0.19159621852558859 86-87 0.19377783837832924 88-89 0.20123170620852648 90-91 0.20125443141532587 92-93 0.1963457867466594 94-95 0.19691391691664395 96-97 0.18561948913735116 98-99 0.1935960367239342 100-101 0.1834605944914099 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2200200.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 49.65951475309367 #Duplication Level Percentage of deduplicated Percentage of total 1 74.39671180456482 36.945046074404445 2 13.67012715755024 13.577037625140651 3 4.784822851827247 7.128359430037647 4 2.2010558377077305 4.372134593801199 5 1.2364596943986368 3.0700994217797395 6 0.7552316094972351 2.250266115229837 7 0.5056150578022713 1.7576018895622743 8 0.37999086506532037 1.5096129575801678 9 0.28306131340136026 1.2651018721996428 >10 1.5901175296085395 14.761001864534432 >50 0.12469132279266205 4.250327244441052 >100 0.06623841073458506 5.9017704524907275 >500 0.0044992298031960795 1.5334564078340112 >1k 0.0011936732130928321 1.0627039857561507 >5k 1.8364203278351264E-4 0.6154800652079515 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7560 0.34360512680665395 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5938 0.26988455594945915 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3725 0.16930279065539497 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 3159 0.1435778565584947 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 4.545041359876375E-5 7 0.0 0.0 0.0 0.0 4.545041359876375E-5 8 0.0 0.0 0.0 0.0 9.09008271975275E-5 9 0.0 0.0 0.0 0.0 1.3635124079629124E-4 10-11 0.0 0.0 0.0 0.0 1.3635124079629124E-4 12-13 0.0 0.0 0.0 4.545041359876375E-5 1.3635124079629124E-4 14-15 0.0 0.0 0.0 4.545041359876375E-5 1.81801654395055E-4 16-17 0.0 0.0 0.0 9.09008271975275E-5 2.2725206799381874E-4 18-19 0.0 0.0 0.0 9.09008271975275E-5 2.2725206799381874E-4 20-21 0.0 0.0 0.0 9.09008271975275E-5 2.2725206799381874E-4 22-23 0.0 0.0 0.0 1.3635124079629124E-4 2.4997727479320064E-4 24-25 0.0 0.0 0.0 1.81801654395055E-4 2.727024815925825E-4 26-27 0.0 0.0 0.0 2.727024815925825E-4 2.727024815925825E-4 28-29 2.2725206799381876E-5 0.0 0.0 9.317334787746569E-4 2.727024815925825E-4 30-31 4.545041359876375E-5 0.0 0.0 0.0026361239887282974 2.727024815925825E-4 32-33 4.545041359876375E-5 0.0 0.0 0.005317698391055359 2.727024815925825E-4 34-35 4.545041359876375E-5 0.0 0.0 0.007976547586583038 3.408781019907281E-4 36-37 4.545041359876375E-5 0.0 0.0 0.009635487682937914 4.0905372238887375E-4 38-39 4.545041359876375E-5 0.0 0.0 0.011362603399690937 4.0905372238887375E-4 40-41 4.545041359876375E-5 0.0 0.0 0.013726024906826652 4.0905372238887375E-4 42-43 4.545041359876375E-5 0.0 0.0 0.015975820379965457 4.0905372238887375E-4 44-45 4.545041359876375E-5 0.0 0.0 0.01863466957549314 4.545041359876375E-4 46-47 4.545041359876375E-5 0.0 0.0 0.02252067993818744 4.999545495864013E-4 48-49 4.545041359876375E-5 0.0 0.0 0.02788382874284156 5.45404963185165E-4 50-51 4.545041359876375E-5 0.0 0.0 0.0358603763294246 5.45404963185165E-4 52-53 4.545041359876375E-5 0.0 0.0 0.05392691573493319 5.45404963185165E-4 54-55 4.545041359876375E-5 0.0 0.0 0.07806108535587673 5.908553767839286E-4 56-57 4.545041359876375E-5 0.0 0.0 0.10583128806472139 6.817562039814562E-4 58-59 4.545041359876375E-5 0.0 0.0 0.13696482137987454 7.2720661758022E-4 60-61 4.545041359876375E-5 0.0 0.0 0.18302881556222161 7.953822379783656E-4 62-63 6.817562039814563E-5 0.0 0.0 0.21836651213526043 8.635578583765112E-4 64-65 9.09008271975275E-5 0.0 0.0 0.2534996818471048 8.635578583765112E-4 66-67 9.09008271975275E-5 0.0 0.0 0.3137442050722662 9.09008271975275E-4 68-69 9.09008271975275E-5 0.0 0.0 0.3897827470229979 9.09008271975275E-4 70-71 9.09008271975275E-5 0.0 0.0 0.46113989637305697 9.09008271975275E-4 72-73 9.09008271975275E-5 0.0 0.0 0.5324061448959185 9.09008271975275E-4 74-75 9.09008271975275E-5 0.0 0.0 0.6417371148077448 9.09008271975275E-4 76-77 9.09008271975275E-5 0.0 0.0 0.7308881010817199 9.09008271975275E-4 78-79 9.09008271975275E-5 0.0 0.0 0.8179710935369512 9.09008271975275E-4 80-81 9.09008271975275E-5 0.0 0.0 0.923234251431688 9.09008271975275E-4 82-83 9.09008271975275E-5 0.0 0.0 1.0750386328515589 9.09008271975275E-4 84-85 9.09008271975275E-5 0.0 0.0 1.237432960639942 9.544586855740388E-4 86-87 9.09008271975275E-5 0.0 0.0 1.4254385964912282 9.544586855740388E-4 88-89 9.09008271975275E-5 0.0 0.0 1.6549177347513861 9.544586855740388E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 10250 0.0 35.47842 1 GGTATCA 6795 0.0 34.629204 1 ATCAACG 12560 0.0 28.828619 3 TCAACGC 12625 0.0 28.64386 4 TATCAAC 12725 0.0 28.571392 2 CAACGCA 12930 0.0 28.005583 5 AACGCAG 13205 0.0 27.413609 6 CGCAGAG 14855 0.0 24.369232 8 ACGCAGA 14870 0.0 24.34465 7 GTACATG 10225 0.0 24.175016 1 TACATGG 10065 0.0 24.081533 2 TAAGGTG 950 0.0 23.010305 5 GAGTACT 7720 0.0 22.949953 12-13 ACATGGG 10210 0.0 22.944557 3 GCAGAGT 15750 0.0 22.410828 9 GTACTTT 7885 0.0 22.288986 14-15 GTATAGG 620 0.0 22.23479 1 GTAAGGT 935 0.0 21.854212 4 AGTACTT 7960 0.0 21.72094 12-13 AGAGTAC 13730 0.0 19.92724 10-11 >>END_MODULE