FastQCFastQC Report
Thu 26 May 2016
SRR937613_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937613_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2200200
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT90040.40923552404326885No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT77570.3525588582856104No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48560.22070720843559677No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA70550.036.031991
GTATCAA107800.034.3801161
ATCAACG135450.027.2480433
TATCAAC136500.027.1776372
TCAACGC137400.026.9304754
CAACGCA139150.026.591795
AACGCAG142450.026.041276
ACCGTAT1151.2822798E-424.7825938
GTAAGGT9600.023.7499834
GTACATG100400.022.7163731
TACATGG98400.022.6879942
ACGCAGA163050.022.664817
CGCAGAG162250.022.6594588
GAGTACT91000.021.9230612-13
ACTGCGT2405.9353624E-921.7708198
GGCGACA5250.021.7132826
TACCGTA1100.002878205621.5908957
GTACACG2657.912604E-1021.5162641
ACATGGG98850.021.4314463
AGTACTT93150.021.41705312-13
Produced by FastQC (version 0.11.5)