##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937613_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2200200 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.20573993273339 33.0 31.0 34.0 31.0 34.0 2 32.415324061448956 34.0 31.0 34.0 31.0 34.0 3 32.48097809290064 34.0 31.0 34.0 31.0 34.0 4 36.09512907917462 37.0 35.0 37.0 35.0 37.0 5 36.011782565221345 37.0 35.0 37.0 35.0 37.0 6 36.05908417416598 37.0 35.0 37.0 35.0 37.0 7 36.01485864921371 37.0 35.0 37.0 35.0 37.0 8 35.971041723479686 37.0 35.0 37.0 35.0 37.0 9 37.66400327242978 39.0 37.0 39.0 35.0 39.0 10-11 37.577642941550764 39.0 37.0 39.0 35.0 39.0 12-13 37.469585037723846 39.0 37.0 39.0 35.0 39.0 14-15 38.688901236251255 40.0 38.0 41.0 34.5 41.0 16-17 38.640106808471955 40.0 38.0 41.0 34.0 41.0 18-19 38.610223843286974 40.0 38.0 41.0 34.0 41.0 20-21 38.487226615762204 40.0 38.0 41.0 34.0 41.0 22-23 38.442247068448324 40.0 38.0 41.0 34.0 41.0 24-25 38.46407576583947 40.0 38.0 41.0 34.0 41.0 26-27 38.279904326879375 40.0 38.0 41.0 34.0 41.0 28-29 38.24692823379693 40.0 38.0 41.0 34.0 41.0 30-31 38.26842150713571 40.0 38.0 41.0 34.0 41.0 32-33 38.13942050722662 40.0 38.0 41.0 34.0 41.0 34-35 37.985953549677305 40.0 37.0 41.0 33.0 41.0 36-37 37.81225593127897 40.0 37.0 41.0 33.0 41.0 38-39 37.6502633851468 40.0 37.0 41.0 33.0 41.0 40-41 37.47329470048177 40.0 37.0 41.0 33.0 41.0 42-43 37.237192982456136 40.0 36.5 41.0 32.0 41.0 44-45 36.922590219071 39.5 36.0 41.0 31.0 41.0 46-47 36.570489728206525 39.0 36.0 41.0 30.5 41.0 48-49 36.21837855649487 39.0 35.5 41.0 30.0 41.0 50-51 35.95532428870103 39.0 35.0 40.0 29.0 41.0 52-53 35.672654304154165 39.0 35.0 40.0 28.0 41.0 54-55 35.43079129170076 38.5 35.0 40.0 28.0 41.0 56-57 35.13041996182166 38.0 34.0 40.0 27.0 41.0 58-59 34.849498454685936 38.0 34.0 40.0 26.5 41.0 60-61 34.471435324061446 37.5 34.0 40.0 26.0 41.0 62-63 34.047691573493324 37.0 33.5 40.0 26.0 41.0 64-65 33.62425484046905 36.0 33.0 39.0 25.0 40.5 66-67 33.17935846741206 36.0 32.5 39.0 23.5 40.0 68-69 32.68298631942551 35.0 32.0 38.5 22.0 40.0 70-71 32.23816425779475 35.0 31.5 38.0 21.5 40.0 72-73 31.748841468957366 35.0 31.0 37.0 20.0 39.0 74-75 31.265204299609124 34.5 31.0 36.5 18.5 39.0 76-77 30.03873556949368 33.5 29.5 35.0 18.0 37.5 78-79 30.648537632942457 34.0 30.0 36.0 18.0 37.5 80-81 30.486532133442417 34.0 30.0 35.0 17.5 37.0 82-83 30.101749840923553 34.0 30.0 35.0 13.5 36.5 84-85 29.68938573766021 34.0 29.5 35.0 8.5 36.0 86-87 29.335959912735206 34.0 29.0 35.0 6.5 36.0 88-89 28.909205072266158 34.0 29.0 35.0 2.0 35.5 90-91 28.374760021816197 33.0 29.0 35.0 2.0 35.0 92-93 27.887183665121352 33.0 27.0 35.0 2.0 35.0 94-95 27.353898736478502 33.0 26.5 35.0 2.0 35.0 96-97 26.77010521770748 33.0 25.5 34.0 2.0 35.0 98-99 25.870895373147896 32.0 24.5 34.0 2.0 35.0 100-101 23.338050631760748 29.5 10.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.25122425312401475 1101 2 0.2487409295503369 1101 3 0.255594741629551 1101 4 0.1629775128126667 1101 5 0.10630744081186094 1101 6 0.13184678599730404 1101 7 0.13114035767382148 1101 8 0.15410455127131684 1101 9 0.23991310871230098 1101 10-11 0.2356376355295211 1101 12-13 0.19064325671759264 1101 14-15 0.2550716289489614 1101 16-17 0.3215012706917193 1101 18-19 0.3099465227425 1101 20-21 0.2660612122884132 1101 22-23 0.1863842252425627 1101 24-25 0.17559743824354257 1101 26-27 0.10720669808635819 1101 28-29 0.24499164206786617 1101 30-31 0.3992788520478143 1101 32-33 0.3675317124248565 1101 34-35 0.3343429031158962 1101 36-37 0.2783439784660544 1101 38-39 0.31704237201437735 1101 40-41 0.36682903751609075 1101 42-43 0.5290375181803384 1101 44-45 0.613425537093633 1101 46-47 0.5852458613670279 1101 48-49 0.44763072108619895 1101 50-51 0.47715958076740606 1101 52-53 0.36702257611570843 1101 54-55 0.41977825736185537 1101 56-57 0.5327924648930633 1101 58-59 0.5954052681966928 1101 60-61 0.6505892669208109 1101 62-63 0.4966415552910277 1101 64-65 0.6693817297754237 1101 66-67 0.6365770658883889 1101 68-69 0.6540038155693253 1101 70-71 0.41946825903800544 1101 72-73 0.5219950353053235 1101 74-75 0.6022368370410618 1101 76-77 0.6695437730242695 1101 78-79 0.6546090753105922 1101 80-81 0.6604920563051913 1101 82-83 0.7419876785172015 1101 84-85 0.7743934505706669 1101 86-87 0.7374923528863278 1101 88-89 0.8544829619234662 1101 90-91 0.8258514411549562 1101 92-93 1.0329575120768126 1101 94-95 1.2533853538422512 1101 96-97 1.2589975872570278 1101 98-99 1.0614819613140973 1101 100-101 1.444999854789522 1102 1 0.1500152867986131 1102 2 0.17851443545126244 1102 3 0.14869623402385201 1102 4 0.11494704876361084 1102 5 0.09105233639810706 1102 6 0.13097044976814942 1102 7 0.09584649304154169 1102 8 0.1346871994642811 1102 9 0.18023010043816612 1102 10-11 0.19879977906332869 1102 12-13 0.22004790409717145 1102 14-15 0.2917563189074315 1102 16-17 0.32871810585903205 1102 18-19 0.33318009851953434 1102 20-21 0.29564114439858 1102 22-23 0.21611435145106128 1102 24-25 0.2254485030480069 1102 26-27 0.26644024309020864 1102 28-29 0.2784124516612607 1102 30-31 0.2555889100167903 1102 32-33 0.348018107007654 1102 34-35 0.21859911711258917 1102 36-37 0.2619296343876343 1102 38-39 0.20451062405930287 1102 40-41 0.35158022761635976 1102 42-43 0.3583288118533332 1102 44-45 0.45351463244258383 1102 46-47 0.3801536344973542 1102 48-49 0.32774330896023685 1102 50-51 0.3921574144055029 1102 52-53 0.4689305831921402 1102 54-55 0.5137417135116493 1102 56-57 0.5455733881793705 1102 58-59 0.49804347386854175 1102 60-61 0.5385317056883068 1102 62-63 0.5466305748610267 1102 64-65 0.4823652489892538 1102 66-67 0.5590647592392486 1102 68-69 0.46458029513855337 1102 70-71 0.5760471801792875 1102 72-73 0.6484533955214786 1102 74-75 0.5051721088049561 1102 76-77 0.45176250528816553 1102 78-79 0.5205646017715075 1102 80-81 0.4972583476439745 1102 82-83 0.4720175459862084 1102 84-85 0.48918132719679264 1102 86-87 0.43160930775431083 1102 88-89 0.6908784014781197 1102 90-91 0.580705298275781 1102 92-93 0.665206300645135 1102 94-95 0.8131876081874765 1102 96-97 0.8821355039057757 1102 98-99 0.913598561591126 1102 100-101 1.2679324653727662 1103 1 0.16775338082130986 1103 2 0.18614796339510775 1103 3 0.20199784287860467 1103 4 0.0761318417243757 1103 5 0.0966771659376775 1103 6 0.12826058472175816 1103 7 0.054653408593800634 1103 8 0.08354451296416698 1103 9 0.14870184885534599 1103 10-11 0.13907381744773062 1103 12-13 0.18265326140411275 1103 14-15 0.3023009828214498 1103 16-17 0.20539673088692467 1103 18-19 0.2151286854663823 1103 20-21 0.17954665353379795 1103 22-23 0.2180895047013749 1103 24-25 0.12884055620103396 1103 26-27 0.14266628200550713 1103 28-29 -0.026824840194706212 1103 30-31 0.0417896004635665 1103 32-33 0.2188762832013893 1103 34-35 0.20614894260485528 1103 36-37 0.23941026206122018 1103 38-39 0.28214185853555307 1103 40-41 0.3948271323466557 1103 42-43 0.45023405627353696 1103 44-45 0.49755786995831386 1103 46-47 0.5486515809505406 1103 48-49 0.6085818794699591 1103 50-51 0.6499925778943734 1103 52-53 0.6944098960415346 1103 54-55 0.7821399542870893 1103 56-57 0.7509802106802894 1103 58-59 0.695706835122138 1103 60-61 0.7781031736388329 1103 62-63 0.6212085622392962 1103 64-65 0.6850025908712638 1103 66-67 0.7169049180390914 1103 68-69 0.926763967982879 1103 70-71 0.7675144415674993 1103 72-73 0.7313751930166745 1103 74-75 0.9126037095067794 1103 76-77 0.6519578875789485 1103 78-79 0.8529313545860191 1103 80-81 0.9107009117772051 1103 82-83 0.9657291969474251 1103 84-85 0.9199568000011489 1103 86-87 1.1152405852558687 1103 88-89 1.2064057057749658 1103 90-91 1.0922185785105398 1103 92-93 1.1518905153166195 1103 94-95 1.1005999350106208 1103 96-97 1.2027950891418229 1103 98-99 1.307234808880164 1103 100-101 1.756030225431573 1104 1 0.18920644169151757 1104 2 0.2070523367753978 1104 3 0.1744952699372675 1104 4 0.11166633153801087 1104 5 0.22161981724523372 1104 6 0.14495525408329968 1104 7 0.16536738431515374 1104 8 0.12958001335475444 1104 9 0.1847462770423789 1104 10-11 0.21946118461575992 1104 12-13 0.31423441135388686 1104 14-15 0.316673030138773 1104 16-17 0.23650917152327366 1104 18-19 0.32598138302488167 1104 20-21 0.3114618208679829 1104 22-23 0.31753988723264825 1104 24-25 0.26249710883163857 1104 26-27 0.2474384681996611 1104 28-29 0.26207152296822045 1104 30-31 0.2819132256449137 1104 32-33 0.3428672562072066 1104 34-35 0.3449978768491633 1104 36-37 0.34077950084257935 1104 38-39 0.3077542495730441 1104 40-41 0.3801125428392993 1104 42-43 0.37461825531379844 1104 44-45 0.3339358505403567 1104 46-47 0.46096310524778517 1104 48-49 0.4234750249062458 1104 50-51 0.38345445496356945 1104 52-53 0.43498533592151034 1104 54-55 0.5479712933325658 1104 56-57 0.5977755037476058 1104 58-59 0.628571665804003 1104 60-61 0.49516177470272993 1104 62-63 0.47274209727469696 1104 64-65 0.5343971746747513 1104 66-67 0.5857027344488728 1104 68-69 0.47866685959694877 1104 70-71 0.4092625847098432 1104 72-73 0.48035066637588386 1104 74-75 0.6305202160104315 1104 76-77 0.5220971984026654 1104 78-79 0.5325087102223129 1104 80-81 0.4959512930685115 1104 82-83 0.41214226872938653 1104 84-85 0.507331407860093 1104 86-87 0.5004664771055367 1104 88-89 0.4850622274062708 1104 90-91 0.561037939429113 1104 92-93 0.5924400707867541 1104 94-95 0.6458379785832236 1104 96-97 0.9939412260099019 1104 98-99 1.137741523327076 1104 100-101 1.3900138206056027 1105 1 0.16644984663069096 1105 2 0.1896390731931774 1105 3 0.1117678069491177 1105 4 0.13063895108793133 1105 5 0.10525773735308519 1105 6 0.04361644790991903 1105 7 0.1527916753870926 1105 8 0.21593820168612154 1105 9 0.15428157593610337 1105 10-11 0.1469685726976664 1105 12-13 0.1884811043677388 1105 14-15 0.1771503851214007 1105 16-17 0.3303120711142853 1105 18-19 0.24503282138945082 1105 20-21 0.28406865807971116 1105 22-23 0.1963511793148811 1105 24-25 0.19161932653378244 1105 26-27 0.21691629722985795 1105 28-29 0.27558408522898503 1105 30-31 0.2984994274984061 1105 32-33 0.22178897027583133 1105 34-35 0.28164591501503367 1105 36-37 0.0756286797484691 1105 38-39 0.1515986493397108 1105 40-41 0.28983841894477536 1105 42-43 0.4058444583776293 1105 44-45 0.4032204463475608 1105 46-47 0.5749108204897553 1105 48-49 0.6347488470577147 1105 50-51 0.5245909840275687 1105 52-53 0.50527759801939 1105 54-55 0.5168117006772803 1105 56-57 0.3365538562473702 1105 58-59 0.4246013492511622 1105 60-61 0.3899915042098314 1105 62-63 0.3916707161320474 1105 64-65 0.525525592818326 1105 66-67 0.4992599698101401 1105 68-69 0.5393526190060385 1105 70-71 0.43973947320947815 1105 72-73 0.49976539412632803 1105 74-75 0.47626847573470954 1105 76-77 0.38514063007005106 1105 78-79 0.36988233956081373 1105 80-81 0.6133124240249934 1105 82-83 0.6290405729166402 1105 84-85 0.6902670189191831 1105 86-87 0.7670603486117464 1105 88-89 0.8932823300493382 1105 90-91 0.9774154006521876 1105 92-93 1.0816615821243936 1105 94-95 1.1385361540043633 1105 96-97 1.328802571296407 1105 98-99 1.3837508874477251 1105 100-101 1.6879496754125611 1106 1 -0.16739745938510708 1106 2 -0.14809504223567416 1106 3 -0.15658946360928638 1106 4 -0.09597815695246226 1106 5 -0.19678679266212384 1106 6 -0.14068448944869516 1106 7 -0.2317169438077329 1106 8 -0.10699783549729602 1106 9 -0.18310249992955363 1106 10-11 -0.34778095842371926 1106 12-13 -0.5082116072355376 1106 14-15 -0.3471875791979855 1106 16-17 -0.3542869604346208 1106 18-19 -0.4083028189661988 1106 20-21 -0.2541501811741469 1106 22-23 -0.3395561531295286 1106 24-25 -0.3963003970620278 1106 26-27 -0.3020072650916319 1106 28-29 -0.49430451499145533 1106 30-31 -0.4115423729556227 1106 32-33 -0.5318633998283531 1106 34-35 -0.6737191824778392 1106 36-37 -0.4173317879542182 1106 38-39 -0.45679689387186073 1106 40-41 -0.5617410691852669 1106 42-43 -0.7524923878082674 1106 44-45 -0.696017638355535 1106 46-47 -0.6767514314430585 1106 48-49 -0.7131489971968463 1106 50-51 -0.8315373990238442 1106 52-53 -0.8168700404467941 1106 54-55 -1.1512775467052734 1106 56-57 -1.0530993479724415 1106 58-59 -1.105277081136549 1106 60-61 -1.0464936301953927 1106 62-63 -1.1030908982055792 1106 64-65 -1.4660213877229609 1106 66-67 -1.3996672897263878 1106 68-69 -1.2950288399090333 1106 70-71 -1.2345520766112443 1106 72-73 -1.210357999179621 1106 74-75 -1.3022507796800653 1106 76-77 -1.2074251524539825 1106 78-79 -1.268772691342324 1106 80-81 -1.2534039706821112 1106 82-83 -1.5749855298200934 1106 84-85 -1.2825831153166298 1106 86-87 -1.2662300332477834 1106 88-89 -1.4005745129874398 1106 90-91 -1.6697982844248145 1106 92-93 -1.4955453114376702 1106 94-95 -1.2196570887621476 1106 96-97 -1.3322061605090099 1106 98-99 -1.2582579210923406 1106 100-101 -0.707159287792674 1107 1 0.12507206710341023 1107 2 0.1557375878627809 1107 3 0.06920167563121993 1107 4 0.07443323545260228 1107 5 0.12112225562667334 1107 6 0.044541717771181766 1107 7 0.05195628638448824 1107 8 0.05717820763444337 1107 9 0.12265816579946431 1107 10-11 0.15713582544876914 1107 12-13 0.14943607208935816 1107 14-15 0.16624713716394268 1107 16-17 0.11426964997325939 1107 18-19 0.2904254979583243 1107 20-21 0.22721409449392382 1107 22-23 0.2540151080050279 1107 24-25 0.12759200800126536 1107 26-27 0.23653000635703592 1107 28-29 0.2443528924159537 1107 30-31 0.2154442655816453 1107 32-33 0.25432682520681027 1107 34-35 0.28440904068202855 1107 36-37 0.4076916532084667 1107 38-39 0.35832265605781544 1107 40-41 0.3163678150153899 1107 42-43 0.4029166604805354 1107 44-45 0.3917724460326468 1107 46-47 0.2567840819184042 1107 48-49 0.3052991978336266 1107 50-51 0.3513798536718937 1107 52-53 0.2989879061073566 1107 54-55 0.3469555756575886 1107 56-57 0.2971321335090451 1107 58-59 0.2463520796698191 1107 60-61 0.37687720899823063 1107 62-63 0.5584157825257918 1107 64-65 0.5145136568152537 1107 66-67 0.6481145902548633 1107 68-69 0.5631081736868353 1107 70-71 0.4794393673643782 1107 72-73 0.4731071906937956 1107 74-75 0.7142547289931649 1107 76-77 0.5499705343720827 1107 78-79 0.7096233471011821 1107 80-81 0.6959191657454582 1107 82-83 0.799457742566215 1107 84-85 0.7543015541629821 1107 86-87 0.8960502058520738 1107 88-89 0.8478900908569109 1107 90-91 0.8999655458611642 1107 92-93 1.0720326304282466 1107 94-95 1.1231552647305847 1107 96-97 1.1047195310052906 1107 98-99 1.1803555341350354 1107 100-101 1.6721040736700559 1108 1 0.20479596520965515 1108 2 0.15499600918002443 1108 3 0.17559307834615367 1108 4 0.12777033952500005 1108 5 0.05809225730255463 1108 6 0.14965525891413733 1108 7 0.14749005544766902 1108 8 0.18737135326876597 1108 9 0.17591147567042498 1108 10-11 0.15667495734083303 1108 12-13 0.22277694690082228 1108 14-15 0.2504299342143881 1108 16-17 0.24117073091845498 1108 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22.0 5 51.0 6 89.0 7 245.0 8 582.0 9 1186.0 10 1929.0 11 3210.0 12 4763.0 13 6845.0 14 9088.0 15 12400.0 16 16598.0 17 22466.0 18 20392.0 19 13422.0 20 11738.0 21 11614.0 22 12377.0 23 13496.0 24 15559.0 25 18281.0 26 22435.0 27 27828.0 28 34842.0 29 43471.0 30 54453.0 31 68544.0 32 87685.0 33 115993.0 34 159875.0 35 228680.0 36 348656.0 37 485768.0 38 307159.0 39 18442.0 40 5.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.38668890244779 18.76184951019751 15.142139563474274 22.70932202388043 2 15.979820016362147 19.84778656485774 39.93450595400418 24.237887464775927 3 20.183665121352604 23.44186892100718 29.31283519680029 27.061630760839922 4 13.416447178545537 16.34777826510832 35.68592783821434 34.549846718131796 5 13.862238527339429 37.38674385486122 33.778320002218194 14.972697615581154 6 33.5258158349241 35.31856194891373 16.547950186346696 14.607672029815472 7 30.049086446686662 32.47932006181256 19.201299881828927 18.270293609671846 8 27.29460958094719 34.95014089628216 18.558221979820015 19.19702754295064 9 26.681483501499866 16.83751477138442 19.56290337242069 36.918098354695026 10-11 25.653145168621034 26.50695391328061 26.066107626579406 21.773793291518952 12-13 26.57040205101493 23.575581177333657 27.071633482668712 22.782383288982704 14-15 23.19936823925098 25.021907099354607 25.612035269520955 26.166689391873465 16-17 22.437278429233707 28.43682392509772 26.34746841196255 22.778429233706028 18-19 22.47029815471321 26.638487410235435 28.979910917189343 21.911303517862013 20-21 23.036891696516264 25.45767492638159 29.517197778198952 21.988235598903188 22-23 22.944265405126927 25.312970347498386 29.36765979514751 22.37510445222718 24-25 22.702009522318317 25.63737970124982 29.449624909013934 22.210985867417932 26-27 22.210124746413197 26.097959556555168 29.36127827519841 22.330637421833224 28-29 22.199486538159547 26.405355783786067 29.25157607605071 22.14358160200367 30-31 22.366353058812834 25.961935278611037 29.166098536496683 22.505613126079446 32-33 22.246482648505285 25.824976326010436 29.283526334771004 22.645014690713275 34-35 22.78604217798382 25.92462048904645 29.138987364785017 22.15034996818471 36-37 22.90374973326246 25.671752500288044 28.967776287254342 22.456721479195156 38-39 22.35992182528861 26.041087173893285 29.15266793927825 22.44632306153986 40-41 22.849656507718635 25.879456139114943 28.878167616961225 22.392719736205198 42-43 22.47441014279587 25.834794295802453 28.811907361227885 22.87888820017379 44-45 22.636464866830288 26.071084446868465 28.946163985092266 22.34628670120898 46-47 22.986433051540768 26.569720934460506 28.144373238796472 22.299472775202254 48-49 22.97575220434506 26.508294700481777 27.96213980547223 22.553813289700937 50-51 23.071970729933643 26.794905008635578 27.874306881192616 22.25881738023816 52-53 23.255060850191473 26.67316153519785 27.59062420958836 22.48115340502232 54-55 22.874670484501408 26.80194982274339 27.729570039087353 22.593809653667847 56-57 22.93189255522225 26.996432142532495 27.697345695845833 22.374329606399417 58-59 23.112580674484136 26.537792018907375 27.6543268793746 22.69530042723389 60-61 22.803086083083357 26.72091173529679 28.007817471138985 22.468184710480866 62-63 22.64812289791837 26.738046541223525 28.27640669030088 22.33742387055722 64-65 22.70082265248614 26.666598491046265 28.074175074993185 22.55840378147441 66-67 22.6911644395964 26.764021452595223 27.770384510499046 22.774429597309336 68-69 22.772907008453778 26.52970184528679 28.0697891100809 22.62760203617853 70-71 22.788519225524954 26.69639123716026 27.818039269157353 22.69705026815744 72-73 22.853172438869194 26.638464685028634 27.71495773111535 22.79340514498682 74-75 22.688732842468866 27.221138987364785 27.28340605399509 22.806722116171258 76-77 22.935210435414962 26.70761748931915 27.664598672847923 22.692573402417963 78-79 22.918552858831013 26.83385601308972 27.57974275065903 22.667848377420235 80-81 22.945868557403873 27.011680756294883 27.403258794655034 22.639191891646213 82-83 22.85215304535161 27.067956399368693 27.412084596950955 22.667805958328742 84-85 22.84965131507632 27.12002183442335 27.427153632484025 22.60317321801631 86-87 22.819720934460502 27.089878192891554 27.386714844105082 22.70368602854286 88-89 22.77499772747932 27.116398509226435 27.23713753295155 22.871466230342698 90-91 22.898453819473406 26.88580455319949 27.3922206648774 22.823520962449706 92-93 22.73909630665923 26.718781318901584 27.642036971132413 22.900085403306775 94-95 22.665575856740297 26.900099990909915 27.532951549859103 22.90137260249068 96-97 22.782860649031907 26.673484228706478 27.456731206253977 23.086923916007635 98-99 22.514287973831344 26.836756561196207 27.255561395961575 23.393394069010874 100-101 22.518780783768875 26.585130423971947 27.770263620770102 23.125825171489073 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 205.0 1 143.0 2 139.0 3 218.0 4 179.5 5 160.5 6 350.0 7 885.5 8 1536.0 9 1917.0 10 1905.5 11 1848.5 12 2039.0 13 2257.0 14 2485.5 15 2748.5 16 2961.5 17 3118.5 18 3296.0 19 3564.0 20 3914.0 21 4387.5 22 4957.0 23 5752.0 24 6917.5 25 8653.5 26 11033.0 27 13713.0 28 16094.0 29 18436.5 30 21760.5 31 26001.0 32 31389.0 33 35128.5 34 37807.5 35 41761.0 36 47020.0 37 52658.5 38 55818.0 39 58376.5 40 61768.0 41 65465.0 42 67837.5 43 70058.0 44 74128.5 45 78885.0 46 82029.5 47 86748.5 48 90621.0 49 88522.0 50 85453.5 51 82028.5 52 79698.0 53 80650.0 54 83065.5 55 77738.5 56 67595.5 57 61290.0 58 56651.0 59 50088.0 60 40464.5 61 33185.0 62 27614.5 63 20702.0 64 14655.5 65 10709.0 66 7746.5 67 5784.5 68 4276.0 69 3152.5 70 2410.5 71 1634.5 72 1110.5 73 859.5 74 643.0 75 456.5 76 363.0 77 247.0 78 159.0 79 103.0 80 62.5 81 38.0 82 29.0 83 19.0 84 8.5 85 4.5 86 2.5 87 1.5 88 0.5 89 1.0 90 1.0 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0337242068902827 2 0.0 3 0.0 4 2.2725206799381872E-4 5 0.009499136442141624 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0053858740114535045 14-15 0.0 16-17 0.0 18-19 0.0 20-21 1.3635124079629124E-4 22-23 0.001431688028361058 24-25 2.2725206799381872E-5 26-27 7.499318243796018E-4 28-29 0.0016589400963548768 30-31 0.0 32-33 2.2725206799381872E-5 34-35 0.0 36-37 1.590764475956731E-4 38-39 0.0 40-41 2.2725206799381872E-4 42-43 0.02052086173984183 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 6.817562039814562E-5 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 1.1362603399690936E-4 84-85 2.0452686119443688E-4 86-87 0.0 88-89 0.0 90-91 0.010476320334515043 92-93 0.002317971093536951 94-95 0.0 96-97 0.0 98-99 4.7722934278701933E-4 100-101 0.012657940187255705 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2200200.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 47.66200793858064 #Duplication Level Percentage of deduplicated Percentage of total 1 72.72762229213872 34.6634451104201 2 14.454822090001452 13.778916904088401 3 5.121062153493583 7.322403150413281 4 2.407503040511696 4.589857161161019 5 1.2979309671474548 3.093099802995582 6 0.8267695862889531 2.364329915104867 7 0.54923844055727 1.832446483980998 8 0.39627112426861844 1.5109661976576934 9 0.2980977032516324 1.2787141588966764 >10 1.7052606624088558 15.145717319266371 >50 0.13695394793379703 4.519165489831736 >100 0.07271474648921877 6.5038724447465555 >500 0.003835497005884688 1.2586752618669006 >1k 0.001725973652648097 1.372381519864963 >5k 1.91774850294233E-4 0.7660090797048401 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9004 0.40923552404326885 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7757 0.3525588582856104 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4856 0.22070720843559677 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 4.545041359876375E-5 9 0.0 0.0 0.0 0.0 4.545041359876375E-5 10-11 0.0 0.0 0.0 0.0 4.545041359876375E-5 12-13 0.0 0.0 0.0 4.545041359876375E-5 4.545041359876375E-5 14-15 0.0 0.0 0.0 4.545041359876375E-5 4.545041359876375E-5 16-17 0.0 0.0 0.0 9.09008271975275E-5 4.545041359876375E-5 18-19 0.0 0.0 0.0 9.09008271975275E-5 4.545041359876375E-5 20-21 0.0 0.0 0.0 9.09008271975275E-5 6.817562039814563E-5 22-23 0.0 0.0 0.0 1.3635124079629124E-4 2.0452686119443685E-4 24-25 0.0 0.0 0.0 1.81801654395055E-4 3.181528951913462E-4 26-27 0.0 0.0 0.0 2.727024815925825E-4 3.181528951913462E-4 28-29 0.0 0.0 0.0 9.317334787746569E-4 3.408781019907281E-4 30-31 0.0 0.0 0.0 0.002727024815925825 3.6360330879011E-4 32-33 0.0 0.0 0.0 0.0054995000454504134 3.863285155894919E-4 34-35 0.0 0.0 0.0 0.008203799654576856 4.545041359876375E-4 36-37 0.0 0.0 0.0 0.009862739750931732 4.545041359876375E-4 38-39 0.0 0.0 0.0 0.011589855467684755 4.545041359876375E-4 40-41 0.0 0.0 0.0 0.014021452595218616 4.545041359876375E-4 42-43 4.545041359876375E-5 0.0 0.0 0.016225797654758657 4.772293427870194E-4 44-45 4.545041359876375E-5 0.0 0.0 0.01890737205708572 5.226797563857831E-4 46-47 4.545041359876375E-5 0.0 0.0 0.02267975638578311 5.45404963185165E-4 48-49 4.545041359876375E-5 0.0 0.0 0.028065630397236613 5.45404963185165E-4 50-51 4.545041359876375E-5 0.0 0.0 0.03615580401781656 5.45404963185165E-4 52-53 4.545041359876375E-5 0.0 0.0 0.054245068630124536 5.908553767839286E-4 54-55 4.545041359876375E-5 0.0 0.0 0.07851558949186438 6.590309971820743E-4 56-57 4.545041359876375E-5 0.0 0.0 0.10621761658031088 6.817562039814562E-4 58-59 4.545041359876375E-5 0.0 0.0 0.13721479865466774 6.817562039814562E-4 60-61 4.545041359876375E-5 0.0 0.0 0.18348331969820925 7.2720661758022E-4 62-63 4.545041359876375E-5 0.0 0.0 0.21870739023725116 7.726570311789837E-4 64-65 4.545041359876375E-5 0.0 0.0 0.2534996818471048 7.726570311789837E-4 66-67 4.545041359876375E-5 0.0 0.0 0.31397145714026 7.726570311789837E-4 68-69 4.545041359876375E-5 0.0 0.0 0.39023725115898555 7.953822379783656E-4 70-71 4.545041359876375E-5 0.0 0.0 0.46170802654304155 8.635578583765112E-4 72-73 4.545041359876375E-5 0.0 0.0 0.5330197254795019 0.0010226343059721844 74-75 4.545041359876375E-5 0.0 0.0 0.6431233524225071 0.0010453595127715661 76-77 4.545041359876375E-5 0.0 0.0 0.7330242705208617 0.0010453595127715661 78-79 4.545041359876375E-5 0.0 0.0 0.8207435687664758 0.0010453595127715661 80-81 4.545041359876375E-5 0.0 0.0 0.9271429870011818 0.0010453595127715661 82-83 4.545041359876375E-5 0.0 0.0 1.079924552313426 0.0010453595127715661 84-85 4.545041359876375E-5 0.0 0.0 1.2443186983001544 0.0010453595127715661 86-87 4.545041359876375E-5 0.0 0.0 1.4358240159985456 0.0010453595127715661 88-89 4.545041359876375E-5 0.0 0.0 1.6703936005817652 0.0010453595127715661 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 7055 0.0 36.03199 1 GTATCAA 10780 0.0 34.380116 1 ATCAACG 13545 0.0 27.248043 3 TATCAAC 13650 0.0 27.177637 2 TCAACGC 13740 0.0 26.930475 4 CAACGCA 13915 0.0 26.59179 5 AACGCAG 14245 0.0 26.04127 6 ACCGTAT 115 1.2822798E-4 24.782593 8 GTAAGGT 960 0.0 23.749983 4 GTACATG 10040 0.0 22.716373 1 TACATGG 9840 0.0 22.687994 2 ACGCAGA 16305 0.0 22.66481 7 CGCAGAG 16225 0.0 22.659458 8 GAGTACT 9100 0.0 21.92306 12-13 ACTGCGT 240 5.9353624E-9 21.770819 8 GGCGACA 525 0.0 21.713282 6 TACCGTA 110 0.0028782056 21.590895 7 GTACACG 265 7.912604E-10 21.516264 1 ACATGGG 9885 0.0 21.431446 3 AGTACTT 9315 0.0 21.417053 12-13 >>END_MODULE