FastQCFastQC Report
Thu 26 May 2016
SRR937612_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937612_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1976821
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT68570.34687005044968666No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54750.2769598259022947No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33320.16855345021122298No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN27590.13956751774692802No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA93950.034.770811
GGTATCA62200.034.5543671
ATCAACG113700.028.5081753
TATCAAC113900.028.4163882
TCAACGC115100.028.1649954
CAACGCA116900.027.5679685
AACGCAG119450.027.0473736
GTACATG95850.025.2015061
TACATGG95350.024.8229942
GTAAGGT7550.023.924194
ACATGGG93550.023.8272843
CGCAGAG136950.023.3471228
ACGCAGA137000.023.3385987
GAGTACT68850.022.97087112-13
TAAGGTG8350.022.7700025
GTACTTT70650.022.3508814-15
GCAGAGT142500.022.0354989
AGTACTT69950.021.48934612-13
AGGACCG6650.021.443185
GTGTAAG7650.020.5119691
Produced by FastQC (version 0.11.5)