##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937612_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1976821 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.729132278542163 33.0 31.0 34.0 30.0 34.0 2 31.82725345390402 33.0 31.0 34.0 30.0 34.0 3 31.87540804149693 33.0 31.0 34.0 30.0 34.0 4 35.497816949536656 37.0 35.0 37.0 33.0 37.0 5 35.49856512046361 37.0 35.0 37.0 33.0 37.0 6 35.55945024865681 37.0 35.0 37.0 33.0 37.0 7 35.479106606010355 37.0 35.0 37.0 33.0 37.0 8 35.473554257062226 37.0 35.0 37.0 33.0 37.0 9 37.03929693179099 39.0 37.0 39.0 33.0 39.0 10-11 36.95166684287551 39.0 37.0 39.0 33.0 39.0 12-13 36.83796711993651 39.0 37.0 39.0 33.0 39.0 14-15 37.94941929491846 40.0 38.0 41.0 33.0 41.0 16-17 37.846277938164356 40.0 38.0 41.0 33.0 41.0 18-19 37.7568568423747 40.0 38.0 41.0 33.0 41.0 20-21 37.754429713160675 40.0 37.5 41.0 32.5 41.0 22-23 37.76907519699558 40.0 38.0 41.0 33.0 41.0 24-25 37.68627736148088 40.0 37.5 41.0 32.0 41.0 26-27 37.568921010045926 40.0 37.0 41.0 32.0 41.0 28-29 37.41783044595338 40.0 37.0 41.0 32.0 41.0 30-31 37.175401313523075 39.5 37.0 41.0 31.5 41.0 32-33 37.028339945801875 39.0 36.0 41.0 31.0 41.0 34-35 37.001189030266275 39.0 36.0 41.0 31.0 41.0 36-37 36.826022437034005 39.0 36.0 41.0 30.5 41.0 38-39 36.63757492458852 39.0 36.0 41.0 30.0 41.0 40-41 36.39039295920065 39.0 35.5 41.0 30.0 41.0 42-43 36.108034819541075 39.0 35.0 40.0 29.5 41.0 44-45 35.8362218936363 39.0 35.0 40.0 28.5 41.0 46-47 35.443863152000105 38.5 35.0 40.0 26.5 41.0 48-49 35.05098463644407 38.0 34.5 40.0 25.5 41.0 50-51 34.16624292234856 37.5 33.5 39.5 24.0 40.5 52-53 34.26500123177566 38.0 33.5 39.5 24.0 40.5 54-55 34.50793622690168 38.0 34.0 40.0 24.0 41.0 56-57 34.22377746897671 38.0 34.0 40.0 23.0 41.0 58-59 33.89164193419637 38.0 33.0 40.0 22.5 41.0 60-61 33.49960213898982 37.0 33.0 40.0 21.5 41.0 62-63 33.08891624481933 36.5 32.5 39.5 19.5 41.0 64-65 32.61630618047866 36.0 32.0 39.0 18.0 40.5 66-67 32.06271508649493 35.5 31.0 39.0 13.0 40.0 68-69 31.54895663289696 35.0 31.0 38.0 10.0 40.0 70-71 31.03728460998745 35.0 30.0 37.5 7.5 40.0 72-73 30.52196936394342 34.5 30.0 37.0 4.5 39.0 74-75 29.93546507245724 34.0 29.0 36.5 2.0 39.0 76-77 29.358082497100142 34.0 29.0 36.0 2.0 38.5 78-79 28.79343501510759 34.0 28.0 35.0 2.0 37.0 80-81 28.207603773938054 33.5 26.5 35.0 2.0 37.0 82-83 27.622661586456235 33.0 26.0 35.0 2.0 36.0 84-85 27.033924922893878 33.0 25.5 35.0 2.0 36.0 86-87 26.40790137296194 32.0 24.5 35.0 2.0 35.5 88-89 25.799355379166855 32.0 22.5 34.0 2.0 35.0 90-91 25.163351664111218 31.5 19.5 34.0 2.0 35.0 92-93 24.4151556969498 31.0 16.5 34.0 2.0 35.0 94-95 23.561680597282205 31.0 4.5 34.0 2.0 35.0 96-97 22.57770733920775 30.0 2.0 34.0 2.0 35.0 98-99 21.279850072414245 29.5 2.0 34.0 2.0 35.0 100-101 18.089788099175394 23.5 2.0 32.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.0635884154807016 1101 2 0.016390372516397633 1101 3 0.02720652208254748 1101 4 0.02721810689934756 1101 5 0.030860770841044882 1101 6 0.012426283582470887 1101 7 -0.037131559748758036 1101 8 -0.044583072317202266 1101 9 0.20727855100546577 1101 10-11 0.10113725377392768 1101 12-13 0.08593714470897851 1101 14-15 0.3206347613953753 1101 16-17 0.2825828738395302 1101 18-19 0.39848542036650514 1101 20-21 0.33684120423821895 1101 22-23 0.24830873037115708 1101 24-25 0.3277660056614877 1101 26-27 0.2701633974961126 1101 28-29 0.2840599211573931 1101 30-31 0.30255644714494423 1101 32-33 0.39198752409898674 1101 34-35 0.37174459415017935 1101 36-37 0.4209035034201136 1101 38-39 0.4202811704399707 1101 40-41 0.5299479358166437 1101 42-43 0.4592322756047835 1101 44-45 0.45437974145515625 1101 46-47 0.37802977103523716 1101 48-49 0.4447310785112535 1101 50-51 0.21156618589605358 1101 52-53 0.3198582494690356 1101 54-55 0.4208924840024366 1101 56-57 0.5947756208836594 1101 58-59 0.4960856802507365 1101 60-61 0.5959808498408918 1101 62-63 0.6207178081477878 1101 64-65 0.6630366741621359 1101 66-67 0.6869218489512292 1101 68-69 0.6912901572482788 1101 70-71 0.7481721656277109 1101 72-73 0.7035368703532612 1101 74-75 0.8587810766734698 1101 76-77 0.8189312661115586 1101 78-79 1.0532537958335055 1101 80-81 1.3117735017691814 1101 82-83 1.316370936836048 1101 84-85 1.5784154265629908 1101 86-87 1.909996622194953 1101 88-89 1.9997392922025874 1101 90-91 2.0309096426375604 1101 92-93 1.9759576631550395 1101 94-95 2.2992838344390556 1101 96-97 2.3398748577947153 1101 98-99 2.7258277054214055 1101 100-101 3.3164623131710336 1102 1 -0.033706989998158576 1102 2 -0.00816830054095874 1102 3 -0.02103585800473695 1102 4 -0.039328113257624864 1102 5 -0.05292299473094886 1102 6 -0.00446209219266791 1102 7 -0.039736446939400594 1102 8 -0.0028646512908991895 1102 9 0.052729125838745006 1102 10-11 0.08641638427209841 1102 12-13 0.011578917486119167 1102 14-15 0.05576927236287332 1102 16-17 0.20908480866814472 1102 18-19 0.014272114879616993 1102 20-21 0.11283907899985479 1102 22-23 0.005371605205276353 1102 24-25 -0.019162108068982775 1102 26-27 0.1559330602901312 1102 28-29 0.09769634326714538 1102 30-31 0.3453366705849348 1102 32-33 0.2946385180175142 1102 34-35 0.1754229431617702 1102 36-37 0.16835992510922893 1102 38-39 0.40973124459257804 1102 40-41 0.4017082388223798 1102 42-43 0.5484656399686187 1102 44-45 0.5352606004859766 1102 46-47 0.6204324216207695 1102 48-49 0.5015023864864361 1102 50-51 0.2669791014564211 1102 52-53 0.43330081096117823 1102 54-55 0.5170420617419893 1102 56-57 0.556185331025894 1102 58-59 0.5794514632187315 1102 60-61 0.44575255211231735 1102 62-63 0.5630993043621544 1102 64-65 0.5500429283825454 1102 66-67 0.9011485515258784 1102 68-69 0.9944549614386382 1102 70-71 0.8053127562307978 1102 72-73 0.6859788443828023 1102 74-75 0.7286220697200889 1102 76-77 0.6307550689854899 1102 78-79 0.8184720278793947 1102 80-81 1.0058389247248982 1102 82-83 1.1835132161133615 1102 84-85 1.3568525881772828 1102 86-87 1.4225223173028816 1102 88-89 1.4953715067160758 1102 90-91 1.611444701209237 1102 92-93 1.7907208209799208 1102 94-95 1.7897311860894334 1102 96-97 1.610536358656514 1102 98-99 2.274510120932053 1102 100-101 2.8537997782770432 1103 1 0.02887332450042024 1103 2 -0.01424519659980561 1103 3 0.08655240968514732 1103 4 0.025310586896978293 1103 5 -0.05690287989514076 1103 6 0.046192785994577434 1103 7 0.016618837343415294 1103 8 -0.057928324572316114 1103 9 -0.20144935035314404 1103 10-11 -0.05222233073836691 1103 12-13 0.07808690940876772 1103 14-15 0.2234886536374816 1103 16-17 0.28306455588505486 1103 18-19 0.22906558127194643 1103 20-21 0.18878086100283298 1103 22-23 0.03676978578675261 1103 24-25 0.04851896630688657 1103 26-27 0.028128512486624402 1103 28-29 -0.010614980400681873 1103 30-31 -0.03378469012704244 1103 32-33 0.0917791892265356 1103 34-35 0.0345206193867682 1103 36-37 0.18106943254193908 1103 38-39 0.20412856883978492 1103 40-41 0.23970199723849106 1103 42-43 0.12132419413255491 1103 44-45 0.2054469035943498 1103 46-47 0.2032656253190268 1103 48-49 0.20916196230915318 1103 50-51 0.21932924561132694 1103 52-53 0.1957711920882943 1103 54-55 0.27214064629677637 1103 56-57 0.13661103633442195 1103 58-59 0.2834008938678565 1103 60-61 0.5677012378461228 1103 62-63 0.6227560915275419 1103 64-65 0.4002074570432157 1103 66-67 0.7171739932489842 1103 68-69 0.7889918246660308 1103 70-71 0.7487646640947503 1103 72-73 0.7938160538578707 1103 74-75 0.900548524719035 1103 76-77 0.8811635521065817 1103 78-79 0.963725208890633 1103 80-81 1.077167749658127 1103 82-83 1.1468070363935219 1103 84-85 1.2339544006984404 1103 86-87 1.3535607738213677 1103 88-89 1.62410560696555 1103 90-91 1.641769651946138 1103 92-93 1.7931829677155058 1103 94-95 2.0006520478567076 1103 96-97 2.0443008656700705 1103 98-99 2.407839245104771 1103 100-101 2.8936644201605937 1104 1 0.15820441754796732 1104 2 0.11716551309072898 1104 3 -0.044121557372271525 1104 4 0.054496580353443846 1104 5 0.04927909791391727 1104 6 0.0621550384765186 1104 7 0.16609650043989888 1104 8 0.11488312596948447 1104 9 0.19058876910425226 1104 10-11 0.18043415949497899 1104 12-13 0.17162673394855688 1104 14-15 0.2892373653650324 1104 16-17 0.32837995828233346 1104 18-19 0.2688949865366155 1104 20-21 0.31480547819723625 1104 22-23 0.26575261819132123 1104 24-25 0.3045654673740472 1104 26-27 0.25132541760532234 1104 28-29 0.3289339566529108 1104 30-31 0.38700725954869597 1104 32-33 0.30636461742170695 1104 34-35 0.41820351082589724 1104 36-37 0.4193539920857532 1104 38-39 0.48789676845876073 1104 40-41 0.5277616632283113 1104 42-43 0.529146705544818 1104 44-45 0.6841894891349938 1104 46-47 0.683376817881971 1104 48-49 0.7768562195225144 1104 50-51 0.7195380290020736 1104 52-53 0.6234705094066086 1104 54-55 0.7561961258065963 1104 56-57 0.8139413336047738 1104 58-59 0.828548707318653 1104 60-61 0.8383494935154943 1104 62-63 1.0224301039961574 1104 64-65 0.9532416819497413 1104 66-67 1.1410543369036361 1104 68-69 1.02356509995953 1104 70-71 1.181932246553913 1104 72-73 1.2218779039100909 1104 74-75 1.3285860473435456 1104 76-77 1.3257542134017477 1104 78-79 1.485265253999092 1104 80-81 1.7549493477430715 1104 82-83 1.8648089004150954 1104 84-85 2.110293436380829 1104 86-87 2.0531038506679273 1104 88-89 2.158426417620312 1104 90-91 2.3258183197762 1104 92-93 2.4283338302039077 1104 94-95 2.72272632327779 1104 96-97 2.893931737500207 1104 98-99 3.300251790705179 1104 100-101 3.7892770496635997 1105 1 0.20732382569053698 1105 2 0.27189221521899043 1105 3 0.09661452565091366 1105 4 0.10564540009411161 1105 5 0.062279794683284706 1105 6 0.1261487732169826 1105 7 0.08714488342513249 1105 8 0.09456493462415239 1105 9 0.28998037048420144 1105 10-11 0.27148506331248257 1105 12-13 0.24836200124174468 1105 14-15 0.28100075834861826 1105 16-17 0.34662554399346135 1105 18-19 0.34239070112175085 1105 20-21 0.32495395238561287 1105 22-23 0.39194080823374833 1105 24-25 0.3332703827137351 1105 26-27 0.33960907071614344 1105 28-29 0.504213230879806 1105 30-31 0.3982617843010061 1105 32-33 0.4806525531149859 1105 34-35 0.5373616944671085 1105 36-37 0.5943592320180002 1105 38-39 0.6251589180205954 1105 40-41 0.6140874227352455 1105 42-43 0.6328520457438813 1105 44-45 0.785074471205931 1105 46-47 0.6892915684328713 1105 48-49 0.6195945231440092 1105 50-51 0.654409919739571 1105 52-53 0.7304203590063949 1105 54-55 0.9175973686618732 1105 56-57 1.007357146304102 1105 58-59 0.8861214007571263 1105 60-61 1.1066035764140523 1105 62-63 1.1164939688568936 1105 64-65 1.1464124924114785 1105 66-67 1.3374186736415083 1105 68-69 1.429884316632144 1105 70-71 1.3561408751645132 1105 72-73 1.2369427091262324 1105 74-75 1.3749013836338975 1105 76-77 1.5746538276294437 1105 78-79 1.770855015454714 1105 80-81 1.9417380623452019 1105 82-83 1.9106292755375947 1105 84-85 2.107677718849999 1105 86-87 2.373183695905823 1105 88-89 2.429837205649477 1105 90-91 2.4354277890418707 1105 92-93 2.6133742484921214 1105 94-95 2.869314136045098 1105 96-97 2.9681780369750825 1105 98-99 3.0604080539820337 1105 100-101 3.589306364960237 1106 1 0.038846425043484345 1106 2 0.009403848868252851 1106 3 0.08528477579811522 1106 4 0.004825188250897838 1106 5 -0.042622482662913797 1106 6 0.03540472616322177 1106 7 0.10990726574325294 1106 8 0.20659460280398179 1106 9 0.29300037086407116 1106 10-11 -0.01317292322730168 1106 12-13 -0.12766445066934295 1106 14-15 -0.2975857295238882 1106 16-17 -0.10379374222279836 1106 18-19 0.19651977606461202 1106 20-21 0.24663970773392663 1106 22-23 0.10290160520995073 1106 24-25 -0.11598474717160201 1106 26-27 0.004516444708691836 1106 28-29 0.26930927575561725 1106 30-31 0.2162940989381852 1106 32-33 0.2180478502716312 1106 34-35 0.16161777403809907 1106 36-37 0.19063203238773951 1106 38-39 -0.030149508223651367 1106 40-41 0.29351311616405695 1106 42-43 0.39899353624385725 1106 44-45 0.3410386553942999 1106 46-47 0.505765607192707 1106 48-49 0.48057953710438994 1106 50-51 0.46770488577248415 1106 52-53 0.6504109254622321 1106 54-55 0.6624342443226965 1106 56-57 0.40540839134200013 1106 58-59 0.2385481641651026 1106 60-61 0.8304737670476214 1106 62-63 0.5701461433204784 1106 64-65 0.7827050462428318 1106 66-67 0.7079844330418155 1106 68-69 0.7734972057658389 1106 70-71 0.7286162941341701 1106 72-73 0.7507329362277275 1106 74-75 0.8173156111912441 1106 76-77 1.1205236447199844 1106 78-79 1.2494008251250506 1106 80-81 1.158865484835026 1106 82-83 1.1214761102353101 1106 84-85 1.3288041093480771 1106 86-87 1.49034245802223 1106 88-89 1.5598847849007456 1106 90-91 1.7103981905056536 1106 92-93 1.7916492394701073 1106 94-95 1.7335486577517436 1106 96-97 1.9602236282177152 1106 98-99 2.127067281444411 1106 100-101 2.648138599823831 1107 1 0.03480832818974733 1107 2 0.03395505363278417 1107 3 0.04606606019363113 1107 4 0.03966046338474172 1107 5 0.09778898112952561 1107 6 0.12206779802499312 1107 7 0.1329160366632962 1107 8 0.2093184753378594 1107 9 0.27304617433325973 1107 10-11 0.17180882001072462 1107 12-13 0.21354615036992186 1107 14-15 0.23105658536889706 1107 16-17 0.27221552954646455 1107 18-19 0.3722996770661098 1107 20-21 0.4041837996316815 1107 22-23 0.329098392504541 1107 24-25 0.5051220589250249 1107 26-27 0.3637457528703081 1107 28-29 0.31684804613038864 1107 30-31 0.28200001360398375 1107 32-33 0.20354493302686905 1107 34-35 0.3274756096020397 1107 36-37 0.3235191454160784 1107 38-39 0.5411982810723046 1107 40-41 0.567257256935001 1107 42-43 0.7453810097624682 1107 44-45 0.6803137670251118 1107 46-47 0.5939244107285404 1107 48-49 0.5487957565409758 1107 50-51 0.5502955283741571 1107 52-53 0.6143116623972347 1107 54-55 0.670927980678087 1107 56-57 0.6985331755022344 1107 58-59 0.6536994085128853 1107 60-61 0.9687823709506134 1107 62-63 1.0093060899234843 1107 64-65 1.0198122178786022 1107 66-67 1.2385448186957149 1107 68-69 1.1026904548030139 1107 70-71 1.3137553605754562 1107 72-73 1.381380617073681 1107 74-75 1.413793207276541 1107 76-77 1.4260393982456172 1107 78-79 1.5976076637364827 1107 80-81 1.859878442289098 1107 82-83 1.9645903927183106 1107 84-85 2.0119563897267057 1107 86-87 2.1031324911227074 1107 88-89 2.1230902401181595 1107 90-91 2.5068413532069265 1107 92-93 2.752678171590901 1107 94-95 2.9454444831330875 1107 96-97 3.1314900800039105 1107 98-99 3.169172696068806 1107 100-101 3.3805793410806793 1108 1 -0.014567254380246197 1108 2 0.07362178699507282 1108 3 0.07197877351795157 1108 4 -0.04492015942223304 1108 5 0.07205341788499453 1108 6 0.11266127519312619 1108 7 0.1362565113083889 1108 8 0.016494517619626947 1108 9 0.1488668965004436 1108 10-11 0.1555579842820407 1108 12-13 0.246899655062208 1108 14-15 0.2408159443100999 1108 16-17 0.4007878121472075 1108 18-19 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-1.1993417766987662 2316 60-61 -1.1711921318954879 2316 62-63 -1.0355392625300048 2316 64-65 -0.9841016764462509 2316 66-67 -1.321440483900286 2316 68-69 -1.2316957864780136 2316 70-71 -1.107975888054277 2316 72-73 -1.4363218376363918 2316 74-75 -1.2772994350893327 2316 76-77 -1.3959565660583628 2316 78-79 -1.426340578665119 2316 80-81 -1.641947568055926 2316 82-83 -1.7237119759185688 2316 84-85 -1.86024521643159 2316 86-87 -2.0375078609653805 2316 88-89 -1.9472238458413536 2316 90-91 -2.3547777160657546 2316 92-93 -2.4867484266502835 2316 94-95 -2.4454303372094195 2316 96-97 -2.5001684124159667 2316 98-99 -3.1176225796496233 2316 100-101 -3.1965383843133495 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 7605.0 3 1617.0 4 1490.0 5 1767.0 6 2042.0 7 2413.0 8 3037.0 9 3732.0 10 4805.0 11 6091.0 12 8023.0 13 9969.0 14 11667.0 15 14302.0 16 17951.0 17 22365.0 18 20088.0 19 15578.0 20 15782.0 21 16927.0 22 18705.0 23 21231.0 24 23775.0 25 27065.0 26 30601.0 27 36549.0 28 42711.0 29 50931.0 30 61234.0 31 74889.0 32 93957.0 33 120939.0 34 158203.0 35 214638.0 36 289263.0 37 333018.0 38 180256.0 39 11605.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.345013974956714 18.01790052916067 14.504523339294087 23.132562156588527 2 15.409066770543115 19.086891532801474 41.0681217484719 24.435919948183507 3 19.81468622097149 23.445687906518344 28.860133088710082 27.879492783800085 4 12.799300855721352 15.726181340293666 36.12274181825191 35.35177598573308 5 13.863056415416944 37.36304703203689 33.60797049662427 15.165926055921902 6 33.355079935236695 34.950945951837106 17.011368048817825 14.682606064108375 7 30.03634280848303 32.28402132922428 19.302593163294272 18.37704269899842 8 27.101011575637024 34.82889995965052 18.75013939832641 19.319949066386044 9 26.801842103262207 17.033879188643237 19.619920466152493 36.544358241942064 10-11 25.478767989046446 26.469139782738676 26.23159654553533 21.820495682679546 12-13 26.40274658704369 23.534038314331628 27.118770372548322 22.944444726076355 14-15 23.24285579591204 24.97777178491807 25.56168697198484 26.21768544718505 16-17 22.452024451563197 28.49101313815336 26.1458629009367 22.91109950934674 18-19 22.521960662771953 26.836318905594553 28.587267263096887 22.054453168536604 20-21 23.141717924493875 25.754777418713935 28.944031318295906 22.159473338496284 22-23 22.92236000996512 25.5898795746222 28.859538700119597 22.628221715293087 24-25 22.600732730699168 25.878126244998462 28.949451921897477 22.571689102404893 26-27 22.164253303869298 26.320324837711667 28.900514775822494 22.61490708259654 28-29 22.260493748308196 26.623929284357946 28.801655154794382 22.313921812539476 30-31 22.383410076110824 26.18692355790787 28.706039973833025 22.723626392148287 32-33 22.156065160931725 26.167883202428694 28.792751628250258 22.88330000838932 34-35 22.73807683241794 26.263334913204258 28.593652974170382 22.40493528020743 36-37 22.79941771616806 25.898173317634104 28.624967750862808 22.67744121533503 38-39 22.351884598289065 26.286407041199183 28.625271340695452 22.736437019816297 40-41 22.753184107312183 26.240485434777433 28.444489268224203 22.561841189686184 42-43 22.39785887446458 26.15385044255368 28.35496135036272 23.093329332619017 44-45 22.535572131864612 26.379207314075327 28.48279465960588 22.60242589445418 46-47 22.886575677420286 26.740731335381618 27.8059558394735 22.5667371477246 48-49 22.825458000746682 26.694687036730542 27.711627253886302 22.768227708636477 50-51 22.919548561565893 26.98421790293516 27.627033589229548 22.4691999462694 52-53 22.965526540974366 26.813830952100933 27.472666182714807 22.747976324209894 54-55 22.677085770057083 26.90363512085685 27.559006625185233 22.860272483900836 56-57 22.869408954794256 27.187400387276533 27.380987975778243 22.56220268215097 58-59 22.965581067294224 26.65727425725693 27.458407426708682 22.91873724874017 60-61 22.633212111629376 26.916184719173096 27.783411478762886 22.667191690434635 62-63 22.576237294628804 26.97096045461133 27.966740279865288 22.48606197089458 64-65 22.52819594120345 26.91769141692998 27.785703245162157 22.768409396704413 66-67 22.537786213989737 27.0276872118159 27.459312465618087 22.975214108576278 68-69 22.60591347322102 26.838246193444547 27.750258643822058 22.805581689512376 70-71 22.632878432596765 26.830671820260065 27.604673708514525 22.93177603862864 72-73 22.635520223816485 26.845408999794735 27.52960525482186 22.989465521566917 74-75 22.50698763450357 27.44677448662523 27.233717842245834 22.812520036625365 76-77 22.777504784008144 27.00610928800831 27.30481994364361 22.91156598433993 78-79 22.78395717480198 27.014349401229776 27.293942760526374 22.90775066344187 80-81 22.80771776602614 27.177609578633778 27.118133374690455 22.89653928064963 82-83 22.775865817374473 27.20257078293638 27.1980857985122 22.823477601176943 84-85 22.756251002423046 27.319977489980836 27.176566066678205 22.747205440917913 86-87 22.80241387838172 27.25431976388264 27.10413651345796 22.839129844277682 88-89 22.72248451120953 27.1704895874327 26.964268021137077 23.142757880220696 90-91 22.84834255928662 26.903335913812832 27.224573935805612 23.02374759109494 92-93 22.781006389229912 26.74884105729529 27.410670508059702 23.0594820454151 94-95 22.677056096281035 26.961821608145254 27.24108546244255 23.12003683313117 96-97 22.74613812289383 26.753119125585574 27.185440549722284 23.315302201798318 98-99 22.512624115703723 26.948872806538514 26.980574053580934 23.557929024176826 100-101 23.06598548519991 25.98361536286494 27.729049970761626 23.22134918117353 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 2408.0 1 1367.0 2 303.5 3 260.5 4 226.0 5 249.0 6 379.0 7 717.0 8 1183.5 9 1486.0 10 1482.0 11 1490.5 12 1640.0 13 1837.0 14 2027.0 15 2136.0 16 2327.5 17 2515.5 18 2660.0 19 2821.5 20 3071.5 21 3540.0 22 4204.0 23 4963.0 24 5946.0 25 7577.5 26 9879.0 27 12373.0 28 14401.0 29 16578.5 30 19495.0 31 23271.0 32 28372.0 33 31924.0 34 34648.5 35 37897.5 36 42448.5 37 47564.5 38 50320.5 39 52662.5 40 55196.0 41 58488.0 42 61159.5 43 63354.0 44 66738.0 45 70359.0 46 73303.0 47 76994.5 48 80446.5 49 79978.5 50 77369.5 51 73695.0 52 71220.5 53 72102.5 54 73730.0 55 69123.5 56 60600.0 57 54599.5 58 50590.5 59 45030.0 60 36783.0 61 30270.0 62 24996.5 63 18881.0 64 13734.0 65 10434.0 66 7520.0 67 5424.5 68 4087.5 69 3040.0 70 2295.5 71 1673.5 72 1166.0 73 878.5 74 658.5 75 448.5 76 320.0 77 218.5 78 143.5 79 91.5 80 70.5 81 42.5 82 24.0 83 19.5 84 12.0 85 10.0 86 7.0 87 4.5 88 4.5 89 5.5 90 4.0 91 1.5 92 1.5 93 4.5 94 7.0 95 3.5 96 2.0 97 2.0 98 1.0 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3112573166715651 2 0.2654767427096333 3 0.27083888728418 4 0.2664884681010572 5 0.26557791524877566 6 0.20735311897232983 7 0.19936048838008094 8 0.20522849565033963 9 0.20618963477219235 10-11 0.2087948276551089 12-13 0.2120070557728798 14-15 0.2075554640506146 16-17 0.19936048838008094 18-19 0.1924554625836128 20-21 0.19167137540525925 22-23 0.19928460897572414 24-25 0.19865228060608423 26-27 0.20338209681099098 28-29 0.20613904850262113 30-31 0.20768192972454258 32-33 0.20611375536783552 34-35 0.2066954974679043 36-37 0.19728645132766195 38-39 0.18959733835284023 40-41 0.18524691916971744 42-43 0.19425127515339022 44-45 0.19516182800567175 46-47 0.19984105794100732 48-49 0.20687254941140343 50-51 0.20315445859792058 52-53 0.21109650292059828 54-55 0.20085278333243123 56-57 0.17978360205602834 58-59 0.1639753928150298 60-61 0.18094708625616582 62-63 0.17416852613362566 64-65 0.18089649998659466 66-67 0.18117472446923621 68-69 0.1794547913038156 70-71 0.18059298236916746 72-73 0.19101375390083372 74-75 0.19584474264488289 76-77 0.194327154557747 78-79 0.1910643401704049 80-81 0.19035613239640817 82-83 0.18059298236916746 84-85 0.1759137524338319 86-87 0.1803400510213115 88-89 0.18719449054820847 90-91 0.1858286612697862 92-93 0.18102296566052264 94-95 0.18198410478237534 96-97 0.18979968343112502 98-99 0.1878015257830628 100-101 0.1713356950376387 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1976821.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 51.05610537675267 #Duplication Level Percentage of deduplicated Percentage of total 1 75.17890390513806 38.38342039889491 2 13.41132337523923 13.694598789758405 3 4.682909815217207 7.172734109866773 4 2.0519246032518423 4.1905311507511 5 1.1682731146189662 2.982373762440649 6 0.7395129844486711 2.2653991716892925 7 0.4732473862206453 1.6913517894107826 8 0.3571368347769748 1.4587212696234504 9 0.2515145680708505 1.1557218862092395 >10 1.5013021144365832 14.296517758385473 >50 0.11779407448738022 4.13457329251862 >100 0.060405775314253174 5.501855273168745 >500 0.004363168039470257 1.4536518034175012 >1k 0.0011899549198555244 0.9941961710681757 >5k 1.9832581997592072E-4 0.624353372796901 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6857 0.34687005044968666 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5475 0.2769598259022947 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3332 0.16855345021122298 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 2759 0.13956751774692802 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 5.058626957119537E-5 2 0.0 0.0 0.0 0.0 5.058626957119537E-5 3 0.0 0.0 0.0 0.0 5.058626957119537E-5 4 0.0 0.0 0.0 0.0 5.058626957119537E-5 5 0.0 0.0 0.0 0.0 5.058626957119537E-5 6 0.0 0.0 0.0 0.0 5.058626957119537E-5 7 0.0 0.0 0.0 0.0 5.058626957119537E-5 8 0.0 0.0 0.0 0.0 5.058626957119537E-5 9 0.0 0.0 0.0 0.0 5.058626957119537E-5 10-11 0.0 0.0 0.0 0.0 5.058626957119537E-5 12-13 0.0 0.0 0.0 0.0 1.0117253914239074E-4 14-15 0.0 0.0 0.0 0.0 1.770519434991838E-4 16-17 0.0 0.0 0.0 0.0 2.2763821307037916E-4 18-19 0.0 0.0 0.0 0.0 2.5293134785597685E-4 20-21 0.0 0.0 0.0 0.0 2.7822448264157453E-4 22-23 0.0 0.0 0.0 5.058626957119537E-5 4.552764261407583E-4 24-25 0.0 0.0 0.0 1.517588087135861E-4 6.070352348543444E-4 26-27 0.0 0.0 0.0 2.5293134785597685E-4 7.082077739967352E-4 28-29 2.5293134785597685E-5 0.0 0.0 6.829146392111375E-4 7.082077739967352E-4 30-31 5.058626957119537E-5 0.0 0.0 0.0024281409394173777 7.082077739967352E-4 32-33 5.058626957119537E-5 0.0 0.0 0.004603350530978779 7.082077739967352E-4 34-35 5.058626957119537E-5 0.0 0.0 0.0069303189312537655 7.082077739967352E-4 36-37 5.058626957119537E-5 0.0 0.0 0.00819497567053365 7.082077739967352E-4 38-39 5.058626957119537E-5 0.0 0.0 0.009333166735885546 7.082077739967352E-4 40-41 5.058626957119537E-5 5.058626957119537E-5 0.0 0.011786600810088522 7.082077739967352E-4 42-43 5.058626957119537E-5 5.058626957119537E-5 0.0 0.014695311310432255 7.082077739967352E-4 44-45 5.058626957119537E-5 5.058626957119537E-5 0.0 0.01823635018041593 7.082077739967352E-4 46-47 5.058626957119537E-5 5.058626957119537E-5 0.0 0.021929147859113193 7.082077739967352E-4 48-49 5.058626957119537E-5 5.058626957119537E-5 0.0 0.02708894735537512 7.082077739967352E-4 50-51 5.058626957119537E-5 5.058626957119537E-5 0.0 0.03429749076927046 7.082077739967352E-4 52-53 5.058626957119537E-5 5.058626957119537E-5 0.0 0.04977688925805624 7.335009087823328E-4 54-55 5.058626957119537E-5 5.058626957119537E-5 0.0 0.07218660667809579 7.587940435679305E-4 56-57 5.058626957119537E-5 5.058626957119537E-5 0.0 0.098263828642047 7.587940435679305E-4 58-59 5.058626957119537E-5 5.058626957119537E-5 0.0 0.1274521061846267 8.093803131391259E-4 60-61 5.058626957119537E-5 5.058626957119537E-5 0.0 0.17262564491170418 8.85259717495919E-4 62-63 5.058626957119537E-5 5.058626957119537E-5 0.0 0.20596199655912195 9.105528522815166E-4 64-65 5.058626957119537E-5 5.058626957119537E-5 0.0 0.2393489344761109 9.105528522815166E-4 66-67 5.058626957119537E-5 5.058626957119537E-5 0.0 0.3024047194966059 9.105528522815166E-4 68-69 5.058626957119537E-5 5.058626957119537E-5 0.0 0.38101578241024353 9.105528522815166E-4 70-71 7.587940435679305E-5 5.058626957119537E-5 0.0 0.450015454105354 9.105528522815166E-4 72-73 1.0117253914239074E-4 5.058626957119537E-5 0.0 0.5227332166139473 9.105528522815166E-4 74-75 1.0117253914239074E-4 5.058626957119537E-5 0.0 0.6384998945276279 9.105528522815166E-4 76-77 1.0117253914239074E-4 5.058626957119537E-5 0.0 0.7350184968694687 9.105528522815166E-4 78-79 1.0117253914239074E-4 7.587940435679305E-5 0.0 0.8234685892147038 9.105528522815166E-4 80-81 1.0117253914239074E-4 1.0117253914239074E-4 0.0 0.9325578795449867 9.105528522815166E-4 82-83 1.0117253914239074E-4 1.0117253914239074E-4 0.0 1.09668503116873 9.105528522815166E-4 84-85 1.0117253914239074E-4 1.0117253914239074E-4 0.0 1.2694624348891477 9.358459870671143E-4 86-87 1.0117253914239074E-4 1.0117253914239074E-4 0.0 1.4691264408866558 9.61139121852712E-4 88-89 1.0117253914239074E-4 1.517588087135861E-4 0.0 1.718896146894433 9.61139121852712E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 9395 0.0 34.77081 1 GGTATCA 6220 0.0 34.554367 1 ATCAACG 11370 0.0 28.508175 3 TATCAAC 11390 0.0 28.416388 2 TCAACGC 11510 0.0 28.164995 4 CAACGCA 11690 0.0 27.567968 5 AACGCAG 11945 0.0 27.047373 6 GTACATG 9585 0.0 25.201506 1 TACATGG 9535 0.0 24.822994 2 GTAAGGT 755 0.0 23.92419 4 ACATGGG 9355 0.0 23.827284 3 CGCAGAG 13695 0.0 23.347122 8 ACGCAGA 13700 0.0 23.338598 7 GAGTACT 6885 0.0 22.970871 12-13 TAAGGTG 835 0.0 22.770002 5 GTACTTT 7065 0.0 22.35088 14-15 GCAGAGT 14250 0.0 22.035498 9 AGTACTT 6995 0.0 21.489346 12-13 AGGACCG 665 0.0 21.44318 5 GTGTAAG 765 0.0 20.511969 1 >>END_MODULE