FastQCFastQC Report
Thu 26 May 2016
SRR937612_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937612_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1976821
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT84110.4254811133633242No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT69430.35122046963280945No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45510.23021811281851012No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20550.10395478396880647No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA71050.035.454951
GTATCAA105150.033.3137321
TATCAAC129050.027.2768672
ATCAACG128400.027.2299653
TCAACGC130050.026.884494
CAACGCA132250.026.4372625
AACGCAG135250.025.952946
GTAAGGT8400.024.8832844
GTACATG89600.023.2874321
TACATGG87450.023.249752
GAGTACT86700.022.79221712-13
ACGCAGA154400.022.5819327
GGACCGA5500.022.453816
CGCAGAG155350.022.4132618
ACATGGG87650.022.058543
GTACTTT89650.021.80213214-15
TAAGGTG10050.021.2706495
AGGACCG5900.020.9341665
GCAGAGT165400.020.9077959
AGTACTT90050.020.78379412-13
Produced by FastQC (version 0.11.5)