##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937612_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1976821 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.19581034398158 33.0 31.0 34.0 31.0 34.0 2 32.42104570924732 34.0 31.0 34.0 31.0 34.0 3 32.53008744848421 34.0 31.0 34.0 31.0 34.0 4 36.15856721473517 37.0 35.0 37.0 35.0 37.0 5 35.9955757248633 37.0 35.0 37.0 35.0 37.0 6 36.094553325769 37.0 35.0 37.0 35.0 37.0 7 36.058965379262965 37.0 35.0 37.0 35.0 37.0 8 36.04449163581326 37.0 35.0 37.0 35.0 37.0 9 37.713175851531325 39.0 37.0 39.0 35.0 39.0 10-11 37.6596550724623 39.0 37.0 39.0 35.0 39.0 12-13 37.57665185669315 39.0 37.0 39.0 35.0 39.0 14-15 38.810904730372656 40.0 38.0 41.0 35.0 41.0 16-17 38.777353134148214 40.0 38.0 41.0 35.0 41.0 18-19 38.75104119189345 40.0 38.0 41.0 35.0 41.0 20-21 38.65952708920029 40.0 38.0 41.0 34.0 41.0 22-23 38.513785264320845 40.0 38.0 41.0 34.0 41.0 24-25 38.45344722663306 40.0 38.0 41.0 34.0 41.0 26-27 38.375941979572254 40.0 38.0 41.0 34.0 41.0 28-29 38.26733932915525 40.0 38.0 41.0 34.0 41.0 30-31 38.341795994680346 40.0 38.0 41.0 34.0 41.0 32-33 38.263543335486624 40.0 38.0 41.0 34.0 41.0 34-35 38.12736661538905 40.0 38.0 41.0 34.0 41.0 36-37 37.964914628082155 40.0 37.0 41.0 33.0 41.0 38-39 37.81317984784662 40.0 37.0 41.0 33.0 41.0 40-41 37.62773108946131 40.0 37.0 41.0 33.0 41.0 42-43 37.41351847233513 40.0 37.0 41.0 33.0 41.0 44-45 37.1068584358422 40.0 36.5 41.0 31.5 41.0 46-47 36.74267574049446 39.5 36.0 41.0 31.0 41.0 48-49 36.40012499867211 39.0 36.0 41.0 30.0 41.0 50-51 36.12931241624811 39.0 35.5 40.5 30.0 41.0 52-53 35.88795444807597 39.0 35.0 40.0 29.0 41.0 54-55 35.66870445022589 39.0 35.0 40.0 28.5 41.0 56-57 35.366705432611255 38.0 35.0 40.0 28.0 41.0 58-59 35.0884652682261 38.0 34.0 40.0 27.5 41.0 60-61 34.716760141661794 38.0 34.0 40.0 26.5 41.0 62-63 34.32704529140474 37.0 34.0 40.0 26.0 41.0 64-65 33.89859982264454 36.5 33.0 39.0 26.0 41.0 66-67 33.448381011735506 36.0 33.0 39.0 25.0 40.0 68-69 32.97706216192564 35.5 32.0 38.5 23.5 40.0 70-71 32.539528111042934 35.0 32.0 38.0 23.0 40.0 72-73 32.08136700287987 35.0 31.0 37.0 22.0 39.0 74-75 31.585627378503162 35.0 31.0 36.5 20.5 39.0 76-77 30.387933201842756 33.5 29.5 35.0 20.0 37.5 78-79 30.958450461625002 34.0 31.0 36.0 20.0 37.5 80-81 30.75782911047586 34.0 31.0 35.0 19.0 37.0 82-83 30.405950007613235 34.0 30.5 35.0 18.0 37.0 84-85 30.009684741309407 34.0 30.0 35.0 15.0 36.0 86-87 29.58142441829584 34.0 29.5 35.0 8.5 36.0 88-89 29.126116628667944 34.0 29.0 35.0 4.5 35.5 90-91 28.594260684199533 33.5 29.0 35.0 2.0 35.0 92-93 28.119335286300583 33.0 28.0 35.0 2.0 35.0 94-95 27.619602634735266 33.0 27.0 35.0 2.0 35.0 96-97 27.022292357274637 33.0 26.0 34.5 2.0 35.0 98-99 26.14302483634077 32.0 24.5 34.0 2.0 35.0 100-101 23.638647859366124 30.5 11.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.2318761978932926 1101 2 0.2219401440829074 1101 3 0.15241737438282144 1101 4 0.1171268737907667 1101 5 -0.004460488507817217 1101 6 0.049446284236907445 1101 7 0.06879084830569582 1101 8 0.13846817500601816 1101 9 0.2932136991392369 1101 10-11 0.2380342702911804 1101 12-13 0.2399897157961135 1101 14-15 0.3065762348901018 1101 16-17 0.23221003483962477 1101 18-19 0.22788078629550768 1101 20-21 0.31276514344765616 1101 22-23 0.3463864601465758 1101 24-25 0.14421738704363207 1101 26-27 0.28981915996721597 1101 28-29 0.24468560276113038 1101 30-31 0.32324921227967707 1101 32-33 0.32530918408384224 1101 34-35 0.29040449120130774 1101 36-37 0.3301314056576672 1101 38-39 0.46346897725508285 1101 40-41 0.2615698925013632 1101 42-43 0.29888903920221566 1101 44-45 0.35572544284521257 1101 46-47 0.4524383395624838 1101 48-49 0.557528988485295 1101 50-51 0.6660765484138409 1101 52-53 0.7586226149530688 1101 54-55 0.695107161844426 1101 56-57 0.7233072983684039 1101 58-59 0.7548891628537078 1101 60-61 0.7754399117678759 1101 62-63 0.7869272381930656 1101 64-65 0.727963150212787 1101 66-67 0.9051156145762747 1101 68-69 0.9064129582516287 1101 70-71 0.7945313252664477 1101 72-73 0.8649799522181425 1101 74-75 0.7287422412311706 1101 76-77 0.6016442510795237 1101 78-79 0.7851935051991674 1101 80-81 0.9162968613404487 1101 82-83 0.7620927625509566 1101 84-85 0.8733815022395142 1101 86-87 1.0821284998615148 1101 88-89 1.0787168117388966 1101 90-91 1.2265205465918143 1101 92-93 1.4555168304751476 1101 94-95 1.3656356211126237 1101 96-97 1.7291655470157075 1101 98-99 1.921840025236211 1101 100-101 2.589792440779039 1102 1 0.10813333621735666 1102 2 0.13004972127522763 1102 3 0.08346882626489815 1102 4 0.08817785194560912 1102 5 3.7173343829266514E-4 1102 6 0.0012241192340383122 1102 7 -0.01397011567204487 1102 8 -0.0019666289985309504 1102 9 0.09965998326980241 1102 10-11 -0.01388315088480141 1102 12-13 0.03024136909394315 1102 14-15 0.03295322416161639 1102 16-17 0.06039672339031199 1102 18-19 0.024061176724345046 1102 20-21 0.14535790362956647 1102 22-23 0.12289818601859537 1102 24-25 0.0037750279065349446 1102 26-27 -0.042331216574460484 1102 28-29 -0.030765954239384996 1102 30-31 0.0349580137844967 1102 32-33 0.10000165445495668 1102 34-35 0.12345872704763394 1102 36-37 0.1018547089331463 1102 38-39 0.031130192042816418 1102 40-41 0.022886715351674525 1102 42-43 0.08012609210427257 1102 44-45 0.17001272941527645 1102 46-47 0.2146682103921762 1102 48-49 0.24214038820020534 1102 50-51 0.2697680882784965 1102 52-53 0.26003236185000844 1102 54-55 0.2647328131938451 1102 56-57 0.30981958985439917 1102 58-59 0.28483270559421925 1102 60-61 0.27112674784784474 1102 62-63 0.364370205620439 1102 64-65 0.36814016084286294 1102 66-67 0.54137692251674 1102 68-69 0.4223446908905899 1102 70-71 0.4455478070944139 1102 72-73 0.35878566238732645 1102 74-75 0.3919373925512808 1102 76-77 0.36719347960860205 1102 78-79 0.393325318984342 1102 80-81 0.39926534643223377 1102 82-83 0.4906520620717352 1102 84-85 0.6546704201056279 1102 86-87 0.6116626360944366 1102 88-89 0.677523243729528 1102 90-91 0.8443159874981205 1102 92-93 1.1588981402615133 1102 94-95 1.041785170265193 1102 96-97 1.2048123939832323 1102 98-99 1.3321402845814454 1102 100-101 1.97352528067373 1103 1 0.18705021661387633 1103 2 0.15654663497010546 1103 3 0.09284542128033024 1103 4 0.050506996279850114 1103 5 -0.08940053682377425 1103 6 0.048048519773523424 1103 7 0.013252612946388354 1103 8 0.02531722087663013 1103 9 0.025667396344445592 1103 10-11 0.1109278348121947 1103 12-13 0.16743598830108652 1103 14-15 0.23622406030252563 1103 16-17 0.2009014406567502 1103 18-19 0.20103809389973293 1103 20-21 0.21029467602398455 1103 22-23 0.169725876070153 1103 24-25 0.14553166980281418 1103 26-27 0.1856546788466389 1103 28-29 0.014263539950377435 1103 30-31 0.10975472724359037 1103 32-33 0.1598403198044025 1103 34-35 0.23553558702796096 1103 36-37 0.17549595474564228 1103 38-39 0.17508401578226085 1103 40-41 0.28971209213345617 1103 42-43 0.3224633859394075 1103 44-45 0.056860941777685525 1103 46-47 0.1745410442219466 1103 48-49 0.28214796468932235 1103 50-51 0.3222501312564958 1103 52-53 0.364608179267222 1103 54-55 0.15278710924356886 1103 56-57 0.3312831256129911 1103 58-59 0.3076281754055117 1103 60-61 0.180295148582573 1103 62-63 0.2662791837600409 1103 64-65 0.31020888193361884 1103 66-67 0.36488186580449167 1103 68-69 0.15167260112723824 1103 70-71 0.3608327902595363 1103 72-73 0.45224197395292975 1103 74-75 0.4405464064510589 1103 76-77 0.4838316194850236 1103 78-79 0.5924667563313442 1103 80-81 0.5548824005362185 1103 82-83 0.5939487898561211 1103 84-85 0.6846847772341604 1103 86-87 0.5942001020453347 1103 88-89 0.7412276163399909 1103 90-91 0.9358047463864381 1103 92-93 1.0255579803063384 1103 94-95 1.0732863868540932 1103 96-97 1.1880733403652428 1103 98-99 1.3861157660439893 1103 100-101 2.240278924869493 1104 1 0.17399968692468804 1104 2 0.19691264922921903 1104 3 0.10097921564545231 1104 4 -0.0021096618451821314 1104 5 -0.06654767559390962 1104 6 -0.025368845901233783 1104 7 0.01798164865040519 1104 8 0.012937263217160933 1104 9 -7.679759516960871E-4 1104 10-11 0.07042374888555258 1104 12-13 0.0702461846288358 1104 14-15 0.06646553975612335 1104 16-17 0.02452402169951995 1104 18-19 0.07508440467235289 1104 20-21 0.14352875345223737 1104 22-23 0.17732879190158002 1104 24-25 -0.005085598129738855 1104 26-27 0.12388496119617542 1104 28-29 0.17536257744922068 1104 30-31 0.2762459120650007 1104 32-33 0.2333406149771875 1104 34-35 0.15979457357975946 1104 36-37 0.22099192071752327 1104 38-39 0.10404684563515332 1104 40-41 0.06381635624498472 1104 42-43 0.08105583340838507 1104 44-45 0.046938054317450906 1104 46-47 0.09287732928316927 1104 48-49 0.17516826349500292 1104 50-51 0.08624056463069962 1104 52-53 0.12146116159362208 1104 54-55 -0.1436823863245209 1104 56-57 0.08499278803479626 1104 58-59 0.12438847563841193 1104 60-61 0.07839793358649416 1104 62-63 0.1738378777339804 1104 64-65 0.1191660245745183 1104 66-67 0.09854518717953908 1104 68-69 0.19536920242340017 1104 70-71 0.2622348069492375 1104 72-73 0.40575972000053895 1104 74-75 0.25206095641527426 1104 76-77 0.011099422296624084 1104 78-79 0.17299692126048782 1104 80-81 0.28667261809910727 1104 82-83 0.36219317130153783 1104 84-85 0.46849330632219477 1104 86-87 0.4715228307826216 1104 88-89 0.5282200110454802 1104 90-91 0.5228482827510774 1104 92-93 0.8944870977505062 1104 94-95 0.980570559321503 1104 96-97 1.3257293872988 1104 98-99 1.5385771611192496 1104 100-101 2.124692179749573 1105 1 0.24996454255747125 1105 2 0.27753962836579404 1105 3 0.13279551778277465 1105 4 0.10697864659138645 1105 5 0.016833808468199152 1105 6 0.12229386107608775 1105 7 0.1176210846637531 1105 8 0.1390596000570028 1105 9 0.18902520250623667 1105 10-11 0.21606341715536104 1105 12-13 0.13113490546223971 1105 14-15 0.34053099075850923 1105 16-17 0.23159269030354324 1105 18-19 0.3392152273700404 1105 20-21 0.2528202862882054 1105 22-23 0.2376928114111294 1105 24-25 0.23975660909966479 1105 26-27 0.25638797769770605 1105 28-29 0.28365215026728663 1105 30-31 0.38953962018094757 1105 32-33 0.3474748365498499 1105 34-35 0.2820896355809026 1105 36-37 0.3429060254821792 1105 38-39 0.23135595631951844 1105 40-41 0.22789138074520565 1105 42-43 0.32394085513286086 1105 44-45 0.42958906350007453 1105 46-47 0.4313054345803238 1105 48-49 0.42726848498836034 1105 50-51 0.37717151648564595 1105 52-53 0.39735279979471727 1105 54-55 0.49459517713531653 1105 56-57 0.5333695419958957 1105 58-59 0.5407173791046986 1105 60-61 0.4375676734026186 1105 62-63 0.47255579377043233 1105 64-65 0.5707964932227299 1105 66-67 0.6294105124235543 1105 68-69 0.5897987295832436 1105 70-71 0.5336314329993428 1105 72-73 0.7501231107182065 1105 74-75 0.6939840965225663 1105 76-77 0.6313247110593778 1105 78-79 0.7634926754991689 1105 80-81 0.7616108718704417 1105 82-83 0.8578744757897532 1105 84-85 0.8485059388075094 1105 86-87 0.9243372408795487 1105 88-89 1.1588678082912587 1105 90-91 1.2250636411341205 1105 92-93 1.4265823908751507 1105 94-95 1.4728876856860111 1105 96-97 1.6454884637111746 1105 98-99 2.1210600676105322 1105 100-101 2.6432507451977436 1106 1 -0.03193698137143741 1106 2 0.11902443022970743 1106 3 -0.01664068550060449 1106 4 0.055111564595527796 1106 5 -0.03585950004668348 1106 6 -0.12658550985213424 1106 7 -0.20183259283566457 1106 8 -0.16532573173150666 1106 9 -0.10671007578331881 1106 10-11 -0.06160330016120952 1106 12-13 -0.1457388243332005 1106 14-15 -0.00787860233285187 1106 16-17 0.06425570384606516 1106 18-19 0.12430769509655448 1106 20-21 -0.0019685036223364705 1106 22-23 0.021973297903429057 1106 24-25 -0.0028722474154960764 1106 26-27 -0.14799054801027722 1106 28-29 0.11740809371666927 1106 30-31 -0.026453561076053234 1106 32-33 0.07581250678099849 1106 34-35 -0.23871718569458977 1106 36-37 0.07356646854219662 1106 38-39 0.047694664873652926 1106 40-41 -0.01115241242592191 1106 42-43 0.11431040947704219 1106 44-45 0.18553726131325732 1106 46-47 0.30784392533988125 1106 48-49 0.23251169948435546 1106 50-51 0.22679512440475236 1106 52-53 0.31075017359731305 1106 54-55 0.2829783056531099 1106 56-57 0.22492272122690338 1106 58-59 0.20894390445813826 1106 60-61 0.289806873893923 1106 62-63 0.2946488059833925 1106 64-65 0.2436432392831236 1106 66-67 0.30723835973942926 1106 68-69 0.13806650295025946 1106 70-71 0.2582154706828703 1106 72-73 0.5430417796387914 1106 74-75 0.4004505061409809 1106 76-77 0.48201354972044896 1106 78-79 0.5998341381417021 1106 80-81 0.4436711515845211 1106 82-83 0.41095478120866247 1106 84-85 0.562648974129484 1106 86-87 0.6648854944076312 1106 88-89 0.7819541916983503 1106 90-91 1.0148910832507738 1106 92-93 1.1320896179829774 1106 94-95 0.9816976029367339 1106 96-97 1.248750950494717 1106 98-99 1.5255414759310462 1106 100-101 2.0769761404252556 1107 1 0.06820256976264005 1107 2 0.0986370395127878 1107 3 -0.0030642383391850103 1107 4 0.055163568093398396 1107 5 -0.1932518730016355 1107 6 -0.017603849415792183 1107 7 -0.03852949221545998 1107 8 0.04700404516843548 1107 9 0.12270310260988282 1107 10-11 0.06258963584520671 1107 12-13 0.028779865622951206 1107 14-15 0.040062564039970994 1107 16-17 -0.03139059360586316 1107 18-19 0.15874638044812883 1107 20-21 0.12082690148996278 1107 22-23 0.08960813842940496 1107 24-25 0.13772072373114952 1107 26-27 0.09869965655027357 1107 28-29 0.06520592229294664 1107 30-31 0.14315837165666068 1107 32-33 0.12124033905306675 1107 34-35 0.19982932158892908 1107 36-37 0.11074265841251929 1107 38-39 -0.023935104553949316 1107 40-41 0.03780888081524125 1107 42-43 0.06647224032329291 1107 44-45 0.0403351646130119 1107 46-47 0.22660385901774305 1107 48-49 0.09618023827695055 1107 50-51 0.16143960223914178 1107 52-53 0.22007399685408302 1107 54-55 0.22491665272949746 1107 56-57 0.2665113826467689 1107 58-59 0.2261646355768292 1107 60-61 0.31674569556534493 1107 62-63 0.1794749426384854 1107 64-65 0.3195468884140027 1107 66-67 0.4794194167332364 1107 68-69 0.39133767443255607 1107 70-71 0.5946919863071471 1107 72-73 0.4121550341691602 1107 74-75 0.6136512154081082 1107 76-77 0.4509083895597712 1107 78-79 0.42012948771628444 1107 80-81 0.44838276946887845 1107 82-83 0.4895191918323185 1107 84-85 0.532518500887555 1107 86-87 0.6557717551844711 1107 88-89 0.7676239462620345 1107 90-91 0.75228881811978 1107 92-93 0.9628939656773525 1107 94-95 1.1880573277927908 1107 96-97 1.4376498698317377 1107 98-99 1.7490260031233227 1107 100-101 2.301865014504088 1108 1 0.10338467321243172 1108 2 0.1343398568334777 1108 3 0.047003351980514196 1108 4 0.03755226367342601 1108 5 -0.044849151820244515 1108 6 0.012570470166856751 1108 7 0.0762788880081473 1108 8 0.12188143096031467 1108 9 0.18294633015745632 1108 10-11 0.051538252561734055 1108 12-13 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#Quality Count 2 1.0 3 9.0 4 11.0 5 32.0 6 80.0 7 168.0 8 406.0 9 892.0 10 1517.0 11 2410.0 12 3850.0 13 5518.0 14 7467.0 15 10413.0 16 14285.0 17 19753.0 18 17684.0 19 11296.0 20 9428.0 21 9656.0 22 10015.0 23 11166.0 24 12608.0 25 15427.0 26 18652.0 27 23175.0 28 29154.0 29 36525.0 30 46301.0 31 59360.0 32 75870.0 33 101519.0 34 141395.0 35 205569.0 36 317414.0 37 450899.0 38 289592.0 39 17304.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.482780171704874 18.75651863678573 15.098845432716029 22.661855758793358 2 15.93083035843913 19.769670597388433 40.11784577359306 24.18165327057938 3 20.188271978090075 23.499193907794382 29.32921088960508 26.983323224510464 4 13.300192581928258 16.329652507738434 35.81851872273717 34.551636187596145 5 13.849307777460723 37.491525560931976 33.6313806201974 15.027786041409904 6 33.470152330433564 35.34356423773321 16.574995915158734 14.611287516674498 7 30.00473993345882 32.51280717879869 19.2376042140386 18.244848673703892 8 27.307935316348825 34.93305666016296 18.55205908880976 19.206948934678454 9 26.81385922144696 16.846340665138623 19.536569067204365 36.80323104621005 10-11 25.5935919337158 26.542817989084494 26.069507557841604 21.794082519358103 12-13 26.558599202027157 23.588763223033258 27.126144360541677 22.726493214397912 14-15 23.183864396422337 25.012735093364547 25.723345715165912 26.080054795047197 16-17 22.398816078946957 28.42647361597231 26.434664544741278 22.740045760339452 18-19 22.408629815243767 26.604937928117923 29.10857887487031 21.877853381768002 20-21 22.969199506884546 25.438418995365787 29.629773894322142 21.96260760342753 22-23 22.87202681685427 25.264788107545645 29.514297356380826 22.34888771921926 24-25 22.66381999497676 25.569686335798835 29.585597817495497 22.180895851728906 26-27 22.16360841185746 26.00503040043384 29.52466661709112 22.306694570617587 28-29 22.201641515795586 26.344408721194494 29.351864569002828 22.102085194007092 30-31 22.29825964074849 26.00469596260025 29.307425190503157 22.389619206148105 32-33 22.220777703191132 25.800059792970632 29.397679405469688 22.581483098368544 34-35 22.773179766908587 25.893568512272985 29.251459793274147 22.081791927544277 36-37 22.814969796195456 25.641149512563228 29.14043030209369 22.403450389147626 38-39 22.294304846012867 25.987810732484125 29.291751756987605 22.426132664515404 40-41 22.784915781449104 25.886107037511234 28.95150850785175 22.377468673187913 42-43 22.391499893252874 25.82248408152445 28.952249126545432 22.833766898677247 44-45 22.589855125982574 26.04631375324321 28.992255747991347 22.371575372782868 46-47 22.895674418675235 26.550077118767963 28.27921698525056 22.27503147730624 48-49 22.918817637004057 26.580125362893252 28.03154660942999 22.4695103906727 50-51 23.032080294573966 26.84808083281187 27.931537554487733 22.188301318126427 52-53 23.159841776226013 26.70158974980278 27.68579645951668 22.452772014454524 54-55 22.77892130850492 26.841226393284977 27.839925820294305 22.539926477915806 56-57 22.88796001256563 27.041876831539124 27.773328996403823 22.29683415949143 58-59 23.026161701034134 26.518030716994613 27.799760322254773 22.656047259716484 60-61 22.689889474059612 26.778929402308048 28.078566546996413 22.45261457663592 62-63 22.545440381299066 26.809028232702907 28.36890644120029 22.27662494479773 64-65 22.607889131084708 26.68724178871026 28.18803523434848 22.516833845856553 66-67 22.59344675112213 26.816995570160373 27.84017875164216 22.74937892707534 68-69 22.747456648831633 26.5977799709736 28.12417006901485 22.53059331117992 70-71 22.724313430502814 26.69371683121537 27.91110070158097 22.670869036700843 72-73 22.75850748095804 26.681451362011394 27.827560338098735 22.73248081893183 74-75 22.56677961746128 27.24608898283328 27.439278092916425 22.74785330678901 76-77 22.892234552344394 26.751359885391746 27.711790799470464 22.644614762793395 78-79 22.866966710693585 26.821649506960927 27.65976282121649 22.651620961129005 80-81 22.89018580842676 27.09446125875838 27.441457774881993 22.573895157932863 82-83 22.690120514351722 27.188510224889136 27.492255864663345 22.6291133960958 84-85 22.70202552891846 27.198395197936886 27.53542690092204 22.56415237222262 86-87 22.762278425815992 27.168038987849684 27.37926195644421 22.690420629890113 88-89 22.73043436912093 27.19806193884019 27.261800638499896 22.80970305353899 90-91 22.835019119232832 26.915534145859628 27.448904713446755 22.800542021460792 92-93 22.654825040324916 26.739144016546305 27.714530198600684 22.89150074452809 94-95 22.571922293419586 26.906432094762245 27.623720104147008 22.897925507671154 96-97 22.683414431554503 26.70365703318611 27.549080063394715 23.063848471864677 98-99 22.410965501123524 26.84364468555021 27.39412530642679 23.351264506899472 100-101 22.367906140791344 26.711500055019343 27.866147418960296 23.054446385229014 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 190.0 1 134.5 2 128.0 3 193.5 4 172.0 5 159.0 6 327.0 7 799.5 8 1367.0 9 1745.5 10 1806.5 11 1789.5 12 1916.0 13 2170.5 14 2428.0 15 2615.0 16 2788.5 17 2943.5 18 3115.5 19 3286.0 20 3588.5 21 4059.0 22 4639.0 23 5244.0 24 6255.0 25 7888.5 26 10149.5 27 12713.5 28 14643.0 29 16682.5 30 19716.0 31 23456.5 32 28339.0 33 31766.5 34 34429.5 35 37992.5 36 42456.0 37 47351.0 38 50314.0 39 52766.0 40 55645.5 41 59070.5 42 61276.0 43 62947.5 44 66955.0 45 71266.0 46 73688.5 47 77785.0 48 81398.0 49 79728.0 50 76759.5 51 73368.5 52 71430.5 53 72781.0 54 74795.5 55 69689.5 56 59911.5 57 54389.5 58 50341.0 59 44138.0 60 35781.0 61 29244.5 62 24637.0 63 18521.5 64 12711.5 65 9242.5 66 6669.0 67 4931.5 68 3694.5 69 2720.5 70 2035.5 71 1377.5 72 946.5 73 729.0 74 567.5 75 397.0 76 279.0 77 191.5 78 131.5 79 83.5 80 50.5 81 32.0 82 24.0 83 14.0 84 5.5 85 3.0 86 3.0 87 2.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.5 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0382432197958237 2 0.0 3 0.0 4 0.0 5 0.014619431906075462 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0031363487134141127 14-15 0.0 16-17 0.0 18-19 0.0 20-21 1.0117253914239075E-4 22-23 2.2763821307037916E-4 24-25 4.80569560926356E-4 26-27 0.0020234507828478148 28-29 0.0025546066133453663 30-31 8.346734479247235E-4 32-33 0.0 34-35 0.0 36-37 2.5293134785597688E-5 38-39 0.0 40-41 0.0 42-43 0.009459632409813533 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 2.5293134785597688E-5 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 1.0117253914239075E-4 74-75 7.587940435679305E-5 76-77 0.0 78-79 0.0 80-81 0.0 82-83 1.2646567392798842E-4 84-85 2.023450782847815E-4 86-87 0.0 88-89 0.0 90-91 0.007410888492180121 92-93 0.0033134006569132965 94-95 0.0 96-97 0.0 98-99 1.517588087135861E-4 100-101 0.012317756640586073 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1976821.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 48.999864942188815 #Duplication Level Percentage of deduplicated Percentage of total 1 73.71384648098751 36.11968521937628 2 14.084680523551427 13.80294886815794 3 4.9250324320709185 7.239777720221241 4 2.269739011749033 4.448676201188788 5 1.2198274164995013 2.988568933062733 6 0.7981254082602159 2.3464822327007933 7 0.5194980450170105 1.7818733830495246 8 0.3642886120497758 1.4280074232333146 9 0.26206360829694825 1.155697327153182 >10 1.6422791306841333 14.97165809716156 >50 0.1283809465333909 4.339746657099786 >100 0.06685001907859715 6.100732934923213 >500 0.0037304066917546353 1.2748039084059533 >1k 0.001450713713460136 1.2217442005840622 >5k 2.0724481620859085E-4 0.7795968936816429 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8411 0.4254811133633242 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6943 0.35122046963280945 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4551 0.23021811281851012 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2055 0.10395478396880647 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 5.058626957119537E-5 7 0.0 0.0 0.0 0.0 5.058626957119537E-5 8 0.0 0.0 0.0 0.0 1.0117253914239074E-4 9 0.0 0.0 0.0 0.0 1.0117253914239074E-4 10-11 0.0 0.0 0.0 0.0 1.0117253914239074E-4 12-13 0.0 0.0 0.0 0.0 1.0117253914239074E-4 14-15 0.0 0.0 0.0 0.0 1.0117253914239074E-4 16-17 0.0 0.0 0.0 0.0 1.0117253914239074E-4 18-19 0.0 0.0 0.0 0.0 1.0117253914239074E-4 20-21 0.0 0.0 0.0 0.0 1.0117253914239074E-4 22-23 0.0 0.0 0.0 5.058626957119537E-5 2.0234507828478148E-4 24-25 0.0 0.0 0.0 1.517588087135861E-4 3.288107522127699E-4 26-27 0.0 0.0 0.0 2.5293134785597685E-4 3.541038869983676E-4 28-29 0.0 0.0 0.0 7.082077739967352E-4 3.541038869983676E-4 30-31 0.0 0.0 0.0 0.002478727208988573 3.541038869983676E-4 32-33 0.0 0.0 0.0 0.004578057396193181 4.552764261407583E-4 34-35 0.0 0.0 0.0 0.006778560122540179 4.552764261407583E-4 36-37 0.0 0.0 0.0 0.008017923727034465 4.552764261407583E-4 38-39 0.0 0.0 0.0 0.009206701061957558 4.552764261407583E-4 40-41 0.0 0.0 0.0 0.011660135136160533 4.552764261407583E-4 42-43 0.0 0.0 0.0 0.014670018175646657 4.552764261407583E-4 44-45 0.0 0.0 0.0 0.01810988450648794 4.552764261407583E-4 46-47 0.0 0.0 0.0 0.021777389050399606 4.80569560926356E-4 48-49 0.0 0.0 0.0 0.02696248168144713 5.058626957119537E-4 50-51 0.0 0.0 0.0 0.03439866330841285 5.058626957119537E-4 52-53 0.0 0.0 0.0 0.04980218239284184 5.058626957119537E-4 54-55 0.0 0.0 0.0 0.07180720965631182 5.058626957119537E-4 56-57 0.0 0.0 0.0 0.09816265610290462 5.058626957119537E-4 58-59 0.0 0.0 0.0 0.1277050375324827 5.058626957119537E-4 60-61 0.0 0.0 0.0 0.17303033506827376 5.058626957119537E-4 62-63 0.0 0.0 0.0 0.2063919798504771 5.058626957119537E-4 64-65 0.0 0.0 0.0 0.23977891776746607 5.058626957119537E-4 66-67 0.0 0.0 0.0 0.3028347027879611 5.564489652831491E-4 68-69 0.0 0.0 0.0 0.38215397347559543 5.564489652831491E-4 70-71 0.0 0.0 0.0 0.4519124392142738 5.564489652831491E-4 72-73 0.0 0.0 0.0 0.5254395820360063 6.576215044255398E-4 74-75 0.0 0.0 0.0 0.642142105936754 6.576215044255398E-4 76-77 0.0 0.0 0.0 0.7393942091873771 7.082077739967352E-4 78-79 0.0 0.0 0.0 0.828046646610897 7.082077739967352E-4 80-81 0.0 0.0 0.0 0.9374141614238214 7.335009087823328E-4 82-83 0.0 0.0 0.0 1.1023254002259182 7.840871783535283E-4 84-85 0.0 0.0 0.0 1.2771515478639697 8.093803131391259E-4 86-87 0.0 0.0 0.0 1.4791678153965382 8.093803131391259E-4 88-89 0.0 0.0 0.0 1.734679063000646 8.093803131391259E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 7105 0.0 35.45495 1 GTATCAA 10515 0.0 33.313732 1 TATCAAC 12905 0.0 27.276867 2 ATCAACG 12840 0.0 27.229965 3 TCAACGC 13005 0.0 26.88449 4 CAACGCA 13225 0.0 26.437262 5 AACGCAG 13525 0.0 25.95294 6 GTAAGGT 840 0.0 24.883284 4 GTACATG 8960 0.0 23.287432 1 TACATGG 8745 0.0 23.24975 2 GAGTACT 8670 0.0 22.792217 12-13 ACGCAGA 15440 0.0 22.581932 7 GGACCGA 550 0.0 22.45381 6 CGCAGAG 15535 0.0 22.413261 8 ACATGGG 8765 0.0 22.05854 3 GTACTTT 8965 0.0 21.802132 14-15 TAAGGTG 1005 0.0 21.270649 5 AGGACCG 590 0.0 20.934166 5 GCAGAGT 16540 0.0 20.907795 9 AGTACTT 9005 0.0 20.783794 12-13 >>END_MODULE