FastQCFastQC Report
Thu 26 May 2016
SRR937611_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937611_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences694919
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33070.47588280072929356No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26600.38277842453580924No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT19650.2827667685010771No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14500.20865741187102382No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT12210.17570393096173798No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN8730.12562615211269226No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT8360.12030179056839718No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC6950.1000116560347321No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGTAAG3000.033.2756731
TAAGGTG3600.027.7277285
GTACATG28500.027.354441
GTGTAGC2900.026.2271351
TACATGG28950.026.1025732
GTATCAA53200.025.5554311
ACATGGG28700.025.5019633
GCACCGT1501.0566364E-625.3400886
GTACACG1151.2743389E-424.8017441
AGGTAAG4100.024.3427812
GTAAGGT4100.023.1869494
GTATGGG1652.4113397E-623.0480861
GAGTACT30000.022.87866412-13
GGTAAGG4400.022.6830483
GGTATCA38400.022.5304031
CATGGGG13000.022.3040624
TCAACGC61550.022.0097084
ATCAACG61650.021.9708333
CAACGCA61550.021.9324825
TATCAAC62350.021.7241692
Produced by FastQC (version 0.11.5)