##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937611_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 694919 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.76468048794176 33.0 31.0 34.0 30.0 34.0 2 31.909282952401647 33.0 31.0 34.0 30.0 34.0 3 31.927948437155987 34.0 31.0 34.0 30.0 34.0 4 35.52051965768673 37.0 35.0 37.0 33.0 37.0 5 35.50591363885575 37.0 35.0 37.0 33.0 37.0 6 35.58164764526513 37.0 35.0 37.0 33.0 37.0 7 35.52591884809596 37.0 35.0 37.0 33.0 37.0 8 35.510837953775905 37.0 35.0 37.0 33.0 37.0 9 37.15614481687794 39.0 37.0 39.0 34.0 39.0 10-11 37.03095396729691 39.0 37.0 39.0 33.0 39.0 12-13 36.91224229010863 39.0 37.0 39.0 33.0 39.0 14-15 38.05887952408842 40.0 38.0 41.0 33.0 41.0 16-17 37.97773193710346 40.0 38.0 41.0 33.0 41.0 18-19 37.925446706738484 40.0 38.0 41.0 33.0 41.0 20-21 37.834808085546655 40.0 38.0 41.0 33.0 41.0 22-23 37.76205284356882 40.0 38.0 41.0 33.0 41.0 24-25 37.78580813015617 40.0 38.0 41.0 33.0 41.0 26-27 37.69692367024071 40.0 37.5 41.0 32.5 41.0 28-29 37.583451452615336 40.0 37.0 41.0 32.0 41.0 30-31 37.3423363010653 40.0 37.0 41.0 32.0 41.0 32-33 37.19526376455386 39.5 36.5 41.0 31.5 41.0 34-35 37.184022886120545 40.0 36.5 41.0 31.5 41.0 36-37 37.04155664185322 40.0 36.0 41.0 31.0 41.0 38-39 36.88027165756009 40.0 36.0 41.0 31.0 41.0 40-41 36.67262803290743 39.0 36.0 41.0 30.0 41.0 42-43 36.40424927221734 39.0 35.5 41.0 30.0 41.0 44-45 36.16601359295112 39.0 35.0 41.0 30.0 41.0 46-47 35.794098304982306 39.0 35.0 40.5 28.5 41.0 48-49 35.4477910375166 39.0 35.0 40.0 27.0 41.0 50-51 34.55033536282646 38.0 34.0 39.5 25.0 40.5 52-53 34.641228689962425 38.0 34.0 39.5 26.0 40.5 54-55 34.922111785690134 38.0 35.0 40.0 26.0 41.0 56-57 34.69086325168833 38.0 34.0 40.0 24.5 41.0 58-59 34.43914542558198 38.0 34.0 40.0 24.0 41.0 60-61 34.097704192862764 38.0 34.0 40.0 23.0 41.0 62-63 33.7283525130267 37.5 33.0 40.0 22.0 41.0 64-65 33.33369140863899 37.0 33.0 39.5 21.5 41.0 66-67 32.87697702897748 36.0 32.0 39.0 19.5 41.0 68-69 32.37249377265552 36.0 32.0 39.0 18.0 40.0 70-71 31.900779803113743 35.0 31.0 38.5 14.5 40.0 72-73 31.443792729800165 35.0 31.0 37.5 10.5 40.0 74-75 30.90665027147049 35.0 30.5 37.0 7.5 39.0 76-77 30.423036353877215 34.5 30.0 36.5 4.5 39.0 78-79 29.857694925595645 34.0 29.0 36.0 2.0 38.5 80-81 29.282786914733947 34.0 29.0 35.5 2.0 37.0 82-83 28.760874288945907 34.0 28.5 35.0 2.0 37.0 84-85 28.25134799883152 34.0 27.5 35.0 2.0 36.0 86-87 27.73609442251543 33.0 26.5 35.0 2.0 36.0 88-89 27.260980776176794 33.0 26.0 35.0 2.0 35.5 90-91 26.69554293378077 32.5 25.0 35.0 2.0 35.0 92-93 26.06311311102445 32.0 24.0 34.5 2.0 35.0 94-95 25.308597117074076 32.0 20.5 34.0 2.0 35.0 96-97 24.472092430916412 31.0 12.5 34.0 2.0 35.0 98-99 23.41579162463539 31.0 2.0 34.0 2.0 35.0 100-101 20.575321728143855 28.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.16430833348463025 1101 2 0.3577882503891239 1101 3 0.15033042372380478 1101 4 0.1319599023097311 1101 5 0.1206879790585873 1101 6 0.0973435801122875 1101 7 0.010248557723627982 1101 8 0.2518853470250093 1101 9 0.27456399951774557 1101 10-11 0.11454380662456032 1101 12-13 0.2327247105561483 1101 14-15 0.3574629495474184 1101 16-17 0.3613222331144712 1101 18-19 0.3913690412740962 1101 20-21 0.2724921324914078 1101 22-23 0.39999111305767343 1101 24-25 0.4353043391925908 1101 26-27 0.4531144297291121 1101 28-29 0.40532900498423174 1101 30-31 0.2713858543829346 1101 32-33 0.25611101243656265 1101 34-35 0.4150213556671858 1101 36-37 0.3668663922251625 1101 38-39 0.32124628172071823 1101 40-41 0.34623388007081957 1101 42-43 0.34292698059161353 1101 44-45 0.33497201669656107 1101 46-47 0.17803228339334254 1101 48-49 0.4255057013931349 1101 50-51 0.28045021963764327 1101 52-53 0.36934208555958037 1101 54-55 0.47773008086421953 1101 56-57 0.3610754118713828 1101 58-59 0.3740136024673646 1101 60-61 0.23872303438491116 1101 62-63 0.5751804741301996 1101 64-65 0.5235088609530933 1101 66-67 0.5873304961359693 1101 68-69 0.33436207139657625 1101 70-71 0.7001551930718399 1101 72-73 0.16799441309887087 1101 74-75 0.13899554594479824 1101 76-77 0.20274745569559016 1101 78-79 0.07873168045662382 1101 80-81 0.14741749248945624 1101 82-83 -0.034626410555222975 1101 84-85 0.054884801708873 1101 86-87 -0.04195995500830918 1101 88-89 -0.10227929241033351 1101 90-91 -0.16728980033848018 1101 92-93 0.06985155686717093 1101 94-95 0.11616319691079369 1101 96-97 0.3226081544571784 1101 98-99 0.4773423792177738 1101 100-101 0.45756137461905055 1102 1 -0.06161382021792505 1102 2 0.1342377054767816 1102 3 0.25903551945402725 1102 4 0.14130691739939039 1102 5 -0.09377237721106013 1102 6 0.09953174144038712 1102 7 0.13957608335491045 1102 8 0.1383768629467852 1102 9 0.10671103926273418 1102 10-11 0.06834366397580283 1102 12-13 0.04031914197174302 1102 14-15 0.12057650792920072 1102 16-17 0.01842370450987829 1102 18-19 0.2573041683811752 1102 20-21 0.16971459115701748 1102 22-23 0.04043261973369994 1102 24-25 0.08285039918137471 1102 26-27 0.010926796729698651 1102 28-29 0.06356124653611772 1102 30-31 0.11521482851537712 1102 32-33 0.07528978418976351 1102 34-35 0.1550469502570948 1102 36-37 0.10293025542325296 1102 38-39 0.19923464087856502 1102 40-41 0.333309639469924 1102 42-43 0.08604329802322752 1102 44-45 0.08302527222860334 1102 46-47 0.09169090087456766 1102 48-49 0.29281815204576844 1102 50-51 0.2931029579946056 1102 52-53 -0.13750270614683302 1102 54-55 -0.1364322099689801 1102 56-57 -0.2526003762922926 1102 58-59 -0.049256928238804676 1102 60-61 0.12331036564993525 1102 62-63 0.300764350712619 1102 64-65 0.14064813720148805 1102 66-67 0.05668009416589115 1102 68-69 0.14444698555783475 1102 70-71 0.1295873571355024 1102 72-73 0.041439145515060005 1102 74-75 0.22435731901588696 1102 76-77 0.008304886491874441 1102 78-79 0.4003282897604912 1102 80-81 0.52910415248353 1102 82-83 0.6364178017507278 1102 84-85 0.3955555443335541 1102 86-87 0.5398355993992716 1102 88-89 0.7314791498418707 1102 90-91 0.785661062158109 1102 92-93 1.0864953782098894 1102 94-95 1.019265513632103 1102 96-97 1.2932448198812914 1102 98-99 1.3410387134972055 1102 100-101 1.3386712757922332 1103 1 0.1401759866138974 1103 2 0.4324588121012489 1103 3 0.13167548453096245 1103 4 0.23934249526689655 1103 5 0.13999385655517216 1103 6 0.10327505694840511 1103 7 0.17189082743436046 1103 8 0.2700258861924425 1103 9 0.23927679283689685 1103 10-11 0.4229218588901418 1103 12-13 0.43633027369716615 1103 14-15 0.5597285323753454 1103 16-17 0.48201095759292656 1103 18-19 0.4231395934602986 1103 20-21 0.40582176129780834 1103 22-23 0.46367828163482017 1103 24-25 0.49115008269114213 1103 26-27 0.42856294209693857 1103 28-29 0.4749603516326957 1103 30-31 0.5248786940615915 1103 32-33 0.4285747345335409 1103 34-35 0.26305742717513425 1103 36-37 0.18407180295135817 1103 38-39 0.4505115152646866 1103 40-41 0.2862999264938324 1103 42-43 0.2885752198828797 1103 44-45 0.22080220118200344 1103 46-47 0.13667071098042527 1103 48-49 0.304326391705267 1103 50-51 0.33418120747621316 1103 52-53 0.30784270474480024 1103 54-55 0.4803417723512169 1103 56-57 0.5653795429481221 1103 58-59 0.4651433773379736 1103 60-61 0.7718970730601669 1103 62-63 0.5859384203404616 1103 64-65 0.6150069716443554 1103 66-67 0.4713586928780984 1103 68-69 0.4414239581650605 1103 70-71 0.6388637949833758 1103 72-73 0.6592226225422344 1103 74-75 0.6318002962067624 1103 76-77 0.5047034459156414 1103 78-79 0.6772408729755952 1103 80-81 0.7301136471905032 1103 82-83 0.9211717033113551 1103 84-85 0.8669732023058323 1103 86-87 1.002151434824352 1103 88-89 1.0220499236059233 1103 90-91 1.0727286164525545 1103 92-93 1.0198960111903546 1103 94-95 1.459137826193487 1103 96-97 1.835037900750006 1103 98-99 2.0127073809641907 1103 100-101 1.6127736440122469 1104 1 0.19319636073518964 1104 2 0.19457009531345548 1104 3 0.1966105494660333 1104 4 0.20070315295782848 1104 5 0.1604871247548587 1104 6 0.08989485916453788 1104 7 0.1653258327959719 1104 8 0.3978347745517894 1104 9 0.3846717648373712 1104 10-11 0.27065211029011493 1104 12-13 0.3504670543477104 1104 14-15 0.1827536723729608 1104 16-17 0.29163207042504524 1104 18-19 0.17465275919364132 1104 20-21 0.35298606488412787 1104 22-23 0.4612119406363675 1104 24-25 0.36935215584734493 1104 26-27 0.4523565796520401 1104 28-29 0.5012739462949156 1104 30-31 0.49571748829488627 1104 32-33 0.24513019741927167 1104 34-35 0.21936625596102743 1104 36-37 0.3229375243858854 1104 38-39 0.4464069042793355 1104 40-41 0.45039500572652713 1104 42-43 0.5130122507419301 1104 44-45 0.47065922489223766 1104 46-47 0.24799562459891433 1104 48-49 0.039230478444217454 1104 50-51 -0.3870626862447466 1104 52-53 -0.1493478111613058 1104 54-55 0.012928451726885726 1104 56-57 0.3117574769407412 1104 58-59 0.02907165089927588 1104 60-61 0.04498178651185469 1104 62-63 0.30277983580573675 1104 64-65 0.3160614217813773 1104 66-67 0.3153119872901158 1104 68-69 0.1631920982818187 1104 70-71 0.185569382973366 1104 72-73 0.1619332147023691 1104 74-75 0.254473952825375 1104 76-77 0.25240986793399145 1104 78-79 0.1565867564499186 1104 80-81 0.5611812034874184 1104 82-83 0.5021677714633839 1104 84-85 0.3536764073576606 1104 86-87 0.4475583215156398 1104 88-89 0.5564238066036573 1104 90-91 0.541583614665349 1104 92-93 0.5770567403694322 1104 94-95 0.5368156529538872 1104 96-97 0.5557039319664945 1104 98-99 0.5919996483298569 1104 100-101 0.6790074102460153 1105 1 0.4321825715782275 1105 2 0.40055831822892785 1105 3 0.25234495590466466 1105 4 0.358073060476336 1105 5 0.29596605337243886 1105 6 0.2146341204201434 1105 7 0.21946719472903453 1105 8 0.39016490210610755 1105 9 0.27050049870848625 1105 10-11 0.4425578566951529 1105 12-13 0.40860025723475957 1105 14-15 0.5082377696169758 1105 16-17 0.245784507986194 1105 18-19 0.4599147900946505 1105 20-21 0.4332225295436416 1105 22-23 0.4367348025786626 1105 24-25 0.5942870865323684 1105 26-27 0.6284166095562398 1105 28-29 0.4241829600102349 1105 30-31 0.5052609878241725 1105 32-33 0.5318763624830467 1105 34-35 0.5040671217059582 1105 36-37 0.5727584683985825 1105 38-39 0.44459419315017357 1105 40-41 0.5270992679973929 1105 42-43 0.2972728602706596 1105 44-45 0.5414167978529392 1105 46-47 0.44470985493506276 1105 48-49 0.42030407599281006 1105 50-51 0.4737092850324558 1105 52-53 0.19885239927562282 1105 54-55 0.30082832804904314 1105 56-57 0.23090908382977915 1105 58-59 0.3789535108664168 1105 60-61 0.23534137523686383 1105 62-63 0.02960039217145294 1105 64-65 0.03216446204128687 1105 66-67 0.26314577903139025 1105 68-69 0.3501856190394008 1105 70-71 0.4240110421024532 1105 72-73 0.2369069100856791 1105 74-75 0.3552409400699439 1105 76-77 0.28287246000014576 1105 78-79 0.19745305515960965 1105 80-81 0.27332747561559856 1105 82-83 0.5795218039149752 1105 84-85 0.32980473696835233 1105 86-87 0.3459597391960365 1105 88-89 0.6444113649814476 1105 90-91 0.47085373077485215 1105 92-93 0.3826360880149373 1105 94-95 0.804830162026672 1105 96-97 1.1267060917139737 1105 98-99 1.0018421898173138 1105 100-101 0.9394701869059752 1106 1 -0.034756747862065396 1106 2 -0.06857294570048822 1106 3 0.11574361868066418 1106 4 0.20796392277902953 1106 5 0.11224020726807993 1106 6 0.0648627351755664 1106 7 0.21583693027116624 1106 8 0.21580881452023704 1106 9 0.11506544138247676 1106 10-11 -0.0869931852826582 1106 12-13 -0.23697219870808084 1106 14-15 -0.09121424426074753 1106 16-17 -0.31784391836308856 1106 18-19 -0.18137831929579562 1106 20-21 -0.2347610653372243 1106 22-23 -0.20876298020285589 1106 24-25 -0.04655568625442896 1106 26-27 -0.01369675579081786 1106 28-29 0.014026261253256678 1106 30-31 0.06758435707720878 1106 32-33 0.1131460359890184 1106 34-35 0.24422519904437223 1106 36-37 0.16877551685572456 1106 38-39 0.013504884049247323 1106 40-41 0.31056318362821145 1106 42-43 0.3243562414714063 1106 44-45 0.09393730425185964 1106 46-47 0.5118100088766582 1106 48-49 0.5822092755424748 1106 50-51 0.5965845463885273 1106 52-53 0.6136434293057249 1106 54-55 0.4970257409112051 1106 56-57 0.6617111884641105 1106 58-59 0.6776886623954894 1106 60-61 0.8905386272645259 1106 62-63 0.9088584855933206 1106 64-65 1.0813350155417325 1106 66-67 1.2460054164644703 1106 68-69 0.7893212484767034 1106 70-71 0.7176121265865127 1106 72-73 1.0041782378893735 1106 74-75 0.7141121116344031 1106 76-77 0.8228111131260825 1106 78-79 0.7541047106061534 1106 80-81 0.7799653452477493 1106 82-83 0.6044856161521821 1106 84-85 0.7795025978694881 1106 86-87 1.2714910674592552 1106 88-89 1.6985080494930855 1106 90-91 1.4441126972982978 1106 92-93 1.739271660011351 1106 94-95 1.5445735161153102 1106 96-97 1.6014803490032214 1106 98-99 1.7993538503186564 1106 100-101 1.7174599854033197 1107 1 -0.0015281036883223464 1107 2 0.15916917339105296 1107 3 0.05219577421013355 1107 4 0.15040775399570805 1107 5 0.20351289203682654 1107 6 0.06371928561693352 1107 7 0.07572624435388775 1107 8 0.15772665893994287 1107 9 0.014500323200607568 1107 10-11 0.22771208424111222 1107 12-13 0.2478021351690316 1107 14-15 0.14676174838051992 1107 16-17 0.20827628105450913 1107 18-19 0.1559570874927445 1107 20-21 0.06756064546659957 1107 22-23 0.06895804859683352 1107 24-25 -0.009133123199539739 1107 26-27 0.23013878058866766 1107 28-29 -0.019500950305243236 1107 30-31 0.20445145774650797 1107 32-33 0.27026871033966415 1107 34-35 0.40846140014919996 1107 36-37 0.2317313085547852 1107 38-39 0.1893026306913086 1107 40-41 0.20156067820317958 1107 42-43 0.3240487805900827 1107 44-45 0.5148690377722076 1107 46-47 0.4330517442773498 1107 48-49 0.3848054142403683 1107 50-51 0.2792048753970775 1107 52-53 0.2234958989026694 1107 54-55 0.44975553615750385 1107 56-57 0.615725458714607 1107 58-59 0.3869259561298932 1107 60-61 0.7265133290782941 1107 62-63 0.7748792303093168 1107 64-65 0.8303567309193554 1107 66-67 0.5978283291608193 1107 68-69 0.7039436814324489 1107 70-71 0.37038461061633754 1107 72-73 0.5837280236831468 1107 74-75 0.4619738609597661 1107 76-77 0.34738257364150016 1107 78-79 0.5347923557607217 1107 80-81 0.6616627834125381 1107 82-83 0.7341223041124216 1107 84-85 0.8159900732853202 1107 86-87 0.44200861813947867 1107 88-89 0.5078289420952231 1107 90-91 0.678481741831849 1107 92-93 0.4184375008533401 1107 94-95 0.8126819456254601 1107 96-97 0.7913412547169116 1107 98-99 0.7006246039281443 1107 100-101 0.8436714066857114 1108 1 0.06833961042969605 1108 2 0.07661465625709596 1108 3 0.2405488977522019 1108 4 0.21109521919353824 1108 5 0.14593316684230473 1108 6 0.08230552728322493 1108 7 -0.012915831285340573 1108 8 0.07108617855708133 1108 9 0.27712316546587346 1108 10-11 0.2838395241556313 1108 12-13 0.28644947816419375 1108 14-15 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-0.35006608013108576 2316 58-59 -0.5760119050386905 2316 60-61 -0.6238350574047828 2316 62-63 -0.5166061089216143 2316 64-65 -0.142349031131495 2316 66-67 -0.10333811158969297 2316 68-69 -0.22103432146878532 2316 70-71 -0.41960254783411344 2316 72-73 -0.41513050524600814 2316 74-75 0.07471196469095176 2316 76-77 -0.4533118212599341 2316 78-79 -0.2453971759983311 2316 80-81 -0.23856551080163158 2316 82-83 -0.44220525052361737 2316 84-85 -0.3481617899283975 2316 86-87 -0.5587404722507614 2316 88-89 -0.7218861657500142 2316 90-91 -0.47257887503814544 2316 92-93 -0.658278867366203 2316 94-95 -0.6517838221327388 2316 96-97 -1.453201086725624 2316 98-99 -1.295362411838422 2316 100-101 -0.927499314581322 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2735.0 3 557.0 4 502.0 5 538.0 6 656.0 7 764.0 8 917.0 9 1166.0 10 1407.0 11 1946.0 12 2477.0 13 3085.0 14 3662.0 15 4710.0 16 5956.0 17 7740.0 18 6675.0 19 4896.0 20 4786.0 21 4946.0 22 5358.0 23 6031.0 24 6805.0 25 7579.0 26 9095.0 27 10361.0 28 12339.0 29 14936.0 30 18224.0 31 22466.0 32 28509.0 33 37315.0 34 50582.0 35 71230.0 36 101176.0 37 132715.0 38 91793.0 39 8283.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.95790401937452 19.412904892555446 15.72822568191101 21.900965406159028 2 14.856986214408865 20.293890624210988 40.807513904619 24.04160925676114 3 19.21801782045659 24.676975369912977 30.015898755799732 26.089108053830707 4 12.467771931317964 17.2215833918861 37.965710921978854 32.34493375481708 5 13.36131640689255 37.7494451963673 34.16551473367983 14.723723663060323 6 30.847113097383406 36.90968067469185 17.543862178332013 14.699344049592733 7 27.987030586824137 33.44698629821 20.596800608968444 17.969182505997416 8 26.276848196856502 34.676235759534734 19.65984354590829 19.387072497700476 9 25.90313158661096 17.80587104010426 20.947981811943336 35.34301556134144 10-11 24.760143052709054 27.28396654798473 26.857490118448858 21.098400280857366 12-13 26.071070483022385 24.564432177849923 27.51269167254728 21.85180566658041 14-15 22.644773451352947 25.889399858722445 26.565081378753586 24.90074531117102 16-17 21.472338683167916 29.51504028235131 27.034518255373385 21.978102779107385 18-19 21.5050453864934 27.71405319803043 29.70229372446041 21.078607691015762 20-21 22.288436727427367 26.603311205771906 29.85224266799288 21.25600939880785 22-23 21.91623240426406 26.510692585465 29.916607203350125 21.65646780692081 24-25 22.023151384974664 26.71757760975645 29.926624813498726 21.33264619177016 26-27 21.40658830584362 27.018987651971848 29.961050231075053 21.613373811109476 28-29 21.203069089105657 27.641625313929985 29.86501371055872 21.290291886405644 30-31 21.404411520877517 26.932437322752676 29.73854984118929 21.924601315180517 32-33 21.3094793542499 27.08039868450837 29.73120659302951 21.87891536821222 34-35 22.14832398952417 27.04274026757632 29.415373347472197 21.393562395427313 36-37 22.014933440961737 26.50145226271901 29.748322510432505 21.735291785886744 38-39 21.497132125455728 27.258176451215142 29.614957484543602 21.629733938785524 40-41 21.746986171740822 27.186615553926753 29.52553510901817 21.540863165314256 42-43 21.577695445961336 27.129326625052258 29.10699612207534 22.185981806911066 44-45 21.717865970441675 27.127906113425375 29.13765028389862 22.016577632234334 46-47 22.162465300979537 27.6231954887489 28.62003320149327 21.594306008778286 48-49 22.003327393618555 27.631135062194 28.41238055694918 21.953156987238263 50-51 21.73933415607483 27.835070125256628 28.58173686881502 21.84385884985352 52-53 22.25228888802366 27.98608136890573 28.01030774710545 21.751321995965153 54-55 21.743467618473744 27.69454431178986 28.37783877133854 22.184149298397863 56-57 22.04429166486502 27.95390668915121 28.161456306481604 21.840345339502168 58-59 22.35056217994082 27.48894881503336 28.141138081079013 22.019350923946803 60-61 21.813791369026816 27.717491170788406 28.749037091696255 21.719680368488525 62-63 21.7496584049674 27.95328363630074 28.65658024456475 21.640477714167115 64-65 21.545685674259328 27.92543685178459 28.435001181849103 22.09387629210698 66-67 21.631610958019625 27.869720745754112 28.164958319401368 22.333709976824895 68-69 21.678751577428592 27.731969387615372 28.517602697718196 22.071676337237836 70-71 21.913924561479366 27.92435969501737 28.061284789783947 22.100430953719318 72-73 21.855173686076576 27.71223253843116 28.33377531931085 22.098818456181416 74-75 21.563998290601987 28.487636771659158 27.96782699623745 21.980537941501403 76-77 21.79864436829076 27.970408166942335 27.963201193474806 22.2677462712921 78-79 21.92026361138078 28.04092549389296 27.881645870225302 22.157165024500955 80-81 22.054749701648284 28.235386531221714 27.745196104996744 21.96466766213326 82-83 22.064283387669096 28.15860854325386 28.01433772343241 21.762770345644636 84-85 21.904846317810186 28.34887665791859 27.815993554960823 21.930283469310403 86-87 22.01125256886578 28.342840178357832 27.636239958725206 22.009667294051184 88-89 21.889383155183683 28.305364811042228 27.45020325130018 22.355048782473908 90-91 21.92171459662803 28.076447717846808 27.821621991690783 22.180215693834384 92-93 22.285026626216915 27.969417682113956 27.869542346133613 21.876013345535515 94-95 21.886989884897105 27.844998803713967 27.897318301446784 22.370693009942148 96-97 21.885814021529676 27.809429646615254 28.069506639205688 22.235249692649383 98-99 21.8726263553646 28.024897054987125 27.43187324611533 22.67060334353295 100-101 22.09816732424729 27.23558198046568 28.105663455137055 22.560587240149978 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 914.0 1 537.5 2 162.0 3 162.0 4 139.0 5 137.5 6 250.5 7 452.5 8 744.5 9 969.0 10 903.5 11 774.0 12 810.5 13 888.5 14 931.5 15 991.5 16 1067.0 17 1124.5 18 1203.5 19 1290.5 20 1414.0 21 1624.0 22 1902.5 23 2267.5 24 2674.5 25 3307.5 26 4223.5 27 5390.0 28 6368.5 29 7329.5 30 8660.0 31 10139.0 32 12069.5 33 13738.0 34 14839.0 35 15879.5 36 17559.0 37 19258.5 38 20008.0 39 20482.5 40 21057.0 41 22540.5 42 23830.0 43 24586.0 44 25478.5 45 26618.0 46 26824.5 47 27886.5 48 30966.0 49 30137.0 50 26459.5 51 23929.5 52 22133.0 53 21153.5 54 20658.0 55 19209.5 56 16978.0 57 15069.5 58 13475.5 59 11590.0 60 9260.0 61 7454.0 62 6233.5 63 4762.5 64 3438.0 65 2641.5 66 1976.5 67 1485.5 68 1076.5 69 733.0 70 539.5 71 399.0 72 312.5 73 254.0 74 180.0 75 136.0 76 107.5 77 68.0 78 38.5 79 30.5 80 26.5 81 14.5 82 9.5 83 7.0 84 4.5 85 4.0 86 5.0 87 3.0 88 1.0 89 1.0 90 1.0 91 0.5 92 0.0 93 1.0 94 1.0 95 1.0 96 1.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.29499840988661985 2 0.2595986007002255 3 0.25657666576967963 4 0.2607498140071001 5 0.27067902877889366 6 0.18261121080298567 7 0.18520144074345354 8 0.18664045737704682 9 0.1837624241098603 10-11 0.19117335977286562 12-13 0.18066853834763477 14-15 0.17973317753579915 16-17 0.18923068731751472 18-19 0.18210755498122802 20-21 0.1746966193182227 22-23 0.1746966193182227 24-25 0.17613563595181597 26-27 0.17340150434798876 28-29 0.1860648507236095 30-31 0.1908136056144673 32-33 0.1925404255747792 34-35 0.19030994979270965 36-37 0.16894055278384965 38-39 0.16009060048725104 40-41 0.1667100770017801 42-43 0.17973317753579915 44-45 0.18325876828810264 46-47 0.1835465716148213 48-49 0.18462583409001623 50-51 0.18750386735720279 52-53 0.2100964285046171 54-55 0.17570393096173798 56-57 0.1595869446654934 58-59 0.1585796330219781 60-61 0.15217600900248807 62-63 0.16045035464564936 64-65 0.1569967147250255 66-67 0.15411868145783897 68-69 0.16512715870482747 70-71 0.1595869446654934 72-73 0.15555769809143224 74-75 0.1593710921704544 76-77 0.16498325704146813 78-79 0.168436896962092 80-81 0.15929914133877474 82-83 0.15613330474486953 84-85 0.15138454985401176 86-87 0.14865041825018455 88-89 0.15447843561623728 90-91 0.1599466988238917 92-93 0.1516723531807304 94-95 0.15901133801205608 96-97 0.15627720640822887 98-99 0.1585796330219781 100-101 0.13828949848831304 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 694919.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 64.47062088875187 #Duplication Level Percentage of deduplicated Percentage of total 1 83.80885157955798 54.032086973073554 2 9.839978934255008 12.687791028473185 3 2.6849937757625266 5.193096474175328 4 1.1263158307191874 2.9045712369318544 5 0.6154328619264049 1.983866936186841 6 0.374818805421794 1.4498880663794 7 0.2674478218113671 1.2069768989266176 8 0.18874947327046648 0.9735036587337478 9 0.1398518842331065 0.8114704028073199 >10 0.8630750373575352 10.228687384835165 >50 0.058312069331832445 2.5414185882467994 >100 0.02904410017988046 3.5918701805959232 >500 0.0020107453968553207 0.8675395635589069 >1k 0.001117080776030734 1.527232607075393 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3307 0.47588280072929356 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2660 0.38277842453580924 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1965 0.2827667685010771 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1450 0.20865741187102382 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 1221 0.17570393096173798 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 873 0.12562615211269226 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 836 0.12030179056839718 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 695 0.1000116560347321 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 7.195083167966338E-5 0.0 12-13 0.0 0.0 0.0 1.4390166335932677E-4 0.0 14-15 0.0 0.0 0.0 1.4390166335932677E-4 0.0 16-17 0.0 0.0 0.0 1.4390166335932677E-4 0.0 18-19 0.0 0.0 0.0 1.4390166335932677E-4 0.0 20-21 0.0 0.0 0.0 1.4390166335932677E-4 0.0 22-23 0.0 0.0 0.0 1.4390166335932677E-4 0.0 24-25 0.0 0.0 0.0 2.8780332671865354E-4 0.0 26-27 0.0 0.0 0.0 2.8780332671865354E-4 0.0 28-29 0.0 0.0 0.0 0.0010792624751949508 0.0 30-31 0.0 0.0 0.0 0.003021934930545862 0.0 32-33 0.0 0.0 0.0 0.005540214039334081 0.0 34-35 0.0 0.0 0.0 0.00827434564316129 0.0 36-37 0.0 0.0 0.0 0.010288968930191864 0.0 38-39 0.0 0.0 0.0 0.012591395543941092 0.0 40-41 0.0 0.0 0.0 0.01676454378136157 0.0 42-43 0.0 0.0 0.0 0.02424743027604656 0.0 44-45 0.0 0.0 0.0 0.037558334136784284 0.0 46-47 1.4390166335932677E-4 0.0 0.0 0.049933877185686384 0.0 48-49 1.4390166335932677E-4 0.0 0.0 0.0774190948873178 0.0 50-51 1.4390166335932677E-4 0.0 0.0 0.09900434439121682 0.0 52-53 1.4390166335932677E-4 0.0 0.0 0.1249785946275753 0.0 54-55 1.4390166335932677E-4 0.0 0.0 0.16635032284338175 0.0 56-57 1.4390166335932677E-4 0.0 0.0 0.20894521519774245 0.0 58-59 1.4390166335932677E-4 0.0 0.0 0.2536266816708134 0.0 60-61 1.4390166335932677E-4 0.0 0.0 0.30435201800497613 0.0 62-63 1.4390166335932677E-4 0.0 0.0 0.3658699790910883 0.0 64-65 1.4390166335932677E-4 0.0 0.0 0.41767457790044593 0.0 66-67 1.4390166335932677E-4 0.0 0.0 0.4884022454415551 0.0 68-69 1.4390166335932677E-4 0.0 0.0 0.5962565421293704 0.0 70-71 1.4390166335932677E-4 0.0 0.0 0.6832450976300835 0.0 72-73 2.1585249503899015E-4 0.0 0.0 0.8040505440202383 0.0 74-75 2.8780332671865354E-4 0.0 0.0 0.9633496853590131 0.0 76-77 2.8780332671865354E-4 0.0 0.0 1.0907746082636969 0.0 78-79 2.8780332671865354E-4 0.0 0.0 1.2152495470695146 0.0 80-81 4.317049900779803E-4 0.0 0.0 1.4012424469614444 0.0 82-83 4.317049900779803E-4 0.0 0.0 1.5997547915656356 0.0 84-85 4.317049900779803E-4 0.0 0.0 1.811074384208807 0.0 86-87 4.317049900779803E-4 0.0 0.0 2.0613193767906766 0.0 88-89 4.317049900779803E-4 0.0 0.0 2.3502019659845246 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTGTAAG 300 0.0 33.275673 1 TAAGGTG 360 0.0 27.727728 5 GTACATG 2850 0.0 27.35444 1 GTGTAGC 290 0.0 26.227135 1 TACATGG 2895 0.0 26.102573 2 GTATCAA 5320 0.0 25.555431 1 ACATGGG 2870 0.0 25.501963 3 GCACCGT 150 1.0566364E-6 25.340088 6 GTACACG 115 1.2743389E-4 24.801744 1 AGGTAAG 410 0.0 24.342781 2 GTAAGGT 410 0.0 23.186949 4 GTATGGG 165 2.4113397E-6 23.048086 1 GAGTACT 3000 0.0 22.878664 12-13 GGTAAGG 440 0.0 22.683048 3 GGTATCA 3840 0.0 22.530403 1 CATGGGG 1300 0.0 22.304062 4 TCAACGC 6155 0.0 22.009708 4 ATCAACG 6165 0.0 21.970833 3 CAACGCA 6155 0.0 21.932482 5 TATCAAC 6235 0.0 21.724169 2 >>END_MODULE