FastQCFastQC Report
Thu 26 May 2016
SRR937611_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937611_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences694919
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46970.6759061127987578No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38580.5551726172402827No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23920.34421277875550965No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT20830.2997471647774777No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT13060.18793557234728075No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT10390.1495138282303405No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10390.1495138282303405No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT7600.10936526415308834No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA7590.10922136248972902No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC7500.10792624751949507No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC7090.10202627932176268No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCACGC500.001613207238.010393
AGGTAAG3050.028.0404552
GTACATG28200.024.606011
GGTAAGG3500.024.4352533
GTAAGGT3750.024.0732484
TAAGGTG4150.024.0427195
TACATGG28750.023.7978132
GTATCAA61600.023.7633381
GAGTACT36350.023.46071212-13
GGTATCA46000.023.1434231
ACATGGG28550.023.1324213
GTACTTT37050.022.75444614-15
CCTATAC1903.2744174E-722.5061533
AGTACTT37200.022.4137912-13
AGAGTAC52800.021.5502710-11
GTATAGG2257.099152E-821.1229671
GCACCGT1150.003723679620.6503936
TACTTTT40700.020.42206814-15
ATCAACG71650.020.3579733
TCAACGC71450.020.3484614
Produced by FastQC (version 0.11.5)