FastQCFastQC Report
Thu 26 May 2016
SRR937610_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937610_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences686441
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32920.47957508365613366No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25250.36783933360623855No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT20460.2980591194290551No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15280.22259742643577526No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT12730.1854492957151452No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN9480.13810363891434224No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT8580.12499253395411988No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC6910.10066415030570727No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACGTG459.594336E-442.247245
CGTATAG600.00393981931.6831171
GTATCAA59300.026.2868351
GAGTACT28550.024.7918612-13
TCAATAC1751.4789657E-724.4376933
GGTCGTT1000.001652183823.7519347
ATATCGT1000.001652183823.7519346
GTACATG29900.023.6828651
GTACTTT30050.023.47443414-15
AGTACTT29950.023.3950612-13
GTGTAGC3900.023.1530481
TATCAAC66850.023.0302852
TACATGG30600.022.9824352
CAACGCA67400.022.9178735
TCAACGC67400.022.9178734
ATCAACG67800.022.7075923
AACGCAG68650.022.5582836
AGAGTAC47100.022.18864310-11
ACATGGG30850.021.7179933
ATTAGCG550.002877505321.59109184-85
Produced by FastQC (version 0.11.5)