##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937610_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 686441 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.73045170670167 33.0 31.0 34.0 30.0 34.0 2 31.862849101379435 33.0 31.0 34.0 30.0 34.0 3 31.900172046832868 33.0 31.0 34.0 30.0 34.0 4 35.47757782533386 37.0 35.0 37.0 33.0 37.0 5 35.462631165679205 37.0 35.0 37.0 33.0 37.0 6 35.55214213603208 37.0 35.0 37.0 33.0 37.0 7 35.49847984021933 37.0 35.0 37.0 33.0 37.0 8 35.4800470834347 37.0 35.0 37.0 33.0 37.0 9 37.1003946442593 39.0 37.0 39.0 33.0 39.0 10-11 36.99348815120309 39.0 37.0 39.0 33.0 39.0 12-13 36.873675232102975 39.0 37.0 39.0 33.0 39.0 14-15 38.00527721974649 40.0 38.0 41.0 33.0 41.0 16-17 37.953022182532806 40.0 38.0 41.0 33.0 41.0 18-19 37.909373857330785 40.0 38.0 41.0 33.0 41.0 20-21 37.72039039043414 40.0 38.0 41.0 32.0 41.0 22-23 37.60354495142336 40.0 37.0 41.0 32.0 41.0 24-25 37.4528364418792 40.0 37.0 41.0 32.0 41.0 26-27 37.553328691030984 40.0 37.0 41.0 32.0 41.0 28-29 37.50951939059584 40.0 37.0 41.0 32.0 41.0 30-31 37.30315788246914 39.5 37.0 41.0 32.0 41.0 32-33 37.06167755131177 39.0 36.0 41.0 31.0 41.0 34-35 36.793586775848176 39.0 36.0 41.0 30.5 41.0 36-37 36.810325286514065 39.0 36.0 41.0 30.0 41.0 38-39 36.75951101405656 39.0 36.0 41.0 30.0 41.0 40-41 36.58511073784928 39.0 36.0 41.0 30.0 41.0 42-43 36.34792356517166 39.0 35.0 41.0 30.0 41.0 44-45 36.10018778015882 39.0 35.0 41.0 29.5 41.0 46-47 35.735857123918876 39.0 35.0 40.5 27.5 41.0 48-49 35.38687447282432 39.0 35.0 40.0 26.5 41.0 50-51 34.475630097852545 38.0 34.0 39.5 25.0 40.5 52-53 34.5785085681071 38.0 34.0 39.5 25.5 40.5 54-55 34.87518228077869 38.0 34.5 40.0 25.5 41.0 56-57 34.611053244197244 38.0 34.0 40.0 24.0 41.0 58-59 34.38135251245191 38.0 34.0 40.0 23.5 41.0 60-61 34.049804717375565 38.0 34.0 40.0 22.5 41.0 62-63 33.697953647873604 37.0 33.0 40.0 22.0 41.0 64-65 33.32396083567269 37.0 33.0 39.5 20.5 41.0 66-67 32.89971315815926 36.0 32.5 39.0 19.5 41.0 68-69 32.413221966636605 36.0 32.0 39.0 18.0 40.0 70-71 31.9458263710938 35.0 31.0 38.5 14.0 40.0 72-73 31.479008392563962 35.0 31.0 37.5 10.0 40.0 74-75 30.987518228077867 35.0 30.5 37.0 7.5 39.0 76-77 30.51904096637584 34.5 30.0 36.5 6.0 39.0 78-79 29.986086204058324 34.0 29.5 36.0 2.0 38.5 80-81 29.438704127521518 34.0 29.0 35.5 2.0 37.0 82-83 28.918289408703735 34.0 29.0 35.0 2.0 37.0 84-85 28.434270388860806 34.0 27.5 35.0 2.0 36.5 86-87 27.928164255922944 33.5 26.5 35.0 2.0 36.0 88-89 27.427839391877818 33.0 26.0 35.0 2.0 35.5 90-91 26.89263243308602 33.0 25.5 35.0 2.0 35.0 92-93 26.333137152355413 32.0 24.5 35.0 2.0 35.0 94-95 25.671260894964025 32.0 22.5 34.0 2.0 35.0 96-97 24.891628705161843 32.0 19.0 34.0 2.0 35.0 98-99 23.9305941807089 31.0 2.0 34.0 2.0 35.0 100-101 21.368765851690092 28.0 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.2999335959063423 1101 2 0.3161344343419543 1101 3 0.2059334276839948 1101 4 0.22348205780613029 1101 5 0.1747335857123673 1101 6 0.06651701708849345 1101 7 0.08750876577257571 1101 8 0.21041805316156115 1101 9 0.3480815965314221 1101 10-11 0.32505340053607057 1101 12-13 0.5044348320511673 1101 14-15 0.5917951220640632 1101 16-17 0.5299776710875292 1101 18-19 0.5311131131401083 1101 20-21 0.4366108509985196 1101 22-23 0.324768279632238 1101 24-25 0.3424588145670384 1101 26-27 0.5384091437358265 1101 28-29 0.39222696742036334 1101 30-31 0.4591377653835096 1101 32-33 0.2566975241374436 1101 34-35 0.41255416055607697 1101 36-37 0.5740174173181245 1101 38-39 0.7277184817968418 1101 40-41 0.5685523585155181 1101 42-43 0.690739054119895 1101 44-45 0.8627785215951818 1101 46-47 0.8242237558921559 1101 48-49 0.8223257912389172 1101 50-51 0.6464138720854393 1101 52-53 0.7975777304496177 1101 54-55 0.8906145138558301 1101 56-57 1.0372918433778224 1101 58-59 1.0649637004652774 1101 60-61 1.0895311853027536 1101 62-63 0.8993360070061911 1101 64-65 1.10515941500136 1101 66-67 1.1228281562015283 1101 68-69 1.3102630023321424 1101 70-71 1.1808184701927864 1101 72-73 1.1607372188759264 1101 74-75 1.1699002566519177 1101 76-77 1.1650818007395465 1101 78-79 1.310230781090418 1101 80-81 1.1522309239040958 1101 82-83 1.4251461465094728 1101 84-85 1.3370106870437297 1101 86-87 1.0054087470183255 1101 88-89 1.281727636241822 1101 90-91 1.3561561531408088 1101 92-93 1.5649071026676182 1101 94-95 1.711148775913859 1101 96-97 1.8419417214638862 1101 98-99 2.2218216774303166 1101 100-101 2.3819311752587673 1102 1 0.3814530405241463 1102 2 0.3755262110534474 1102 3 0.4323106553498235 1102 4 0.3178189558942819 1102 5 0.4161089716493933 1102 6 0.3984503917842446 1102 7 0.44431220986661657 1102 8 0.45633718597039774 1102 9 0.42729551486145567 1102 10-11 0.4135053326380884 1102 12-13 0.5630428032492247 1102 14-15 0.6185754497082314 1102 16-17 0.7828042047091728 1102 18-19 0.7057909941607576 1102 20-21 0.6256566641554357 1102 22-23 0.732650634157082 1102 24-25 0.7054757988554456 1102 26-27 0.7699401526972807 1102 28-29 0.6721275263024538 1102 30-31 0.5307143219502777 1102 32-33 0.47980369460199057 1102 34-35 0.6429770728861186 1102 36-37 0.7478472464717711 1102 38-39 0.8418004258792635 1102 40-41 0.7673587224533662 1102 42-43 0.7815381196719784 1102 44-45 0.7424132841345426 1102 46-47 0.77637245989024 1102 48-49 0.7560168237940701 1102 50-51 0.6354785308394 1102 52-53 0.7932071427973995 1102 54-55 0.7943838780127308 1102 56-57 1.0097026235488258 1102 58-59 0.9078091653457676 1102 60-61 1.0858679194097363 1102 62-63 0.8530780205725748 1102 64-65 0.8310850825821561 1102 66-67 0.8116714403772249 1102 68-69 0.8437898089701932 1102 70-71 1.018197488190328 1102 72-73 1.1021298595479045 1102 74-75 0.9548481314437254 1102 76-77 1.0727114529403856 1102 78-79 1.0429934844301272 1102 80-81 1.4291351552301315 1102 82-83 1.3729236509853031 1102 84-85 1.2512589374013672 1102 86-87 1.4768276858643645 1102 88-89 1.419380037770516 1102 90-91 1.5210269812579504 1102 92-93 1.6464981360793942 1102 94-95 1.6408063612054846 1102 96-97 1.5013760843297383 1102 98-99 2.01073997687217 1102 100-101 2.2644753798093724 1103 1 -0.04298547789873908 1103 2 0.1500567800456487 1103 3 -0.018179048299789002 1103 4 -0.06077985827383969 1103 5 0.12752741107957632 1103 6 0.11140995542823617 1103 7 0.10821534227554963 1103 8 0.05621642423008666 1103 9 0.06034254335611422 1103 10-11 0.15789791318737656 1103 12-13 0.07102545013677997 1103 14-15 0.2986536624344751 1103 16-17 0.07800479964229368 1103 18-19 0.1683918362654495 1103 20-21 0.07120293620823759 1103 22-23 0.042963928841274424 1103 24-25 0.10743824437481919 1103 26-27 -0.20172252668030666 1103 28-29 -0.08349199621800096 1103 30-31 -0.17684861737433977 1103 32-33 -0.11764753338891154 1103 34-35 0.1197337998395156 1103 36-37 0.09675386859914425 1103 38-39 0.16750680809689555 1103 40-41 0.029693431502288092 1103 42-43 0.229049795772319 1103 44-45 0.11296422931704342 1103 46-47 0.21991096715141367 1103 48-49 0.18642282799249443 1103 50-51 -0.02973106070889031 1103 52-53 -0.22796717771608854 1103 54-55 -0.10922592977707524 1103 56-57 0.40922899008626956 1103 58-59 0.27775495704118214 1103 60-61 0.42802439058493746 1103 62-63 0.021043750058211685 1103 64-65 0.2422209825731514 1103 66-67 0.48817185120920215 1103 68-69 0.5629797639602288 1103 70-71 0.6621708990908921 1103 72-73 0.5767456879425161 1103 74-75 0.4429535034584724 1103 76-77 0.5029063258110824 1103 78-79 0.5040639606052295 1103 80-81 0.9147170372036832 1103 82-83 0.7071405084097968 1103 84-85 1.0408376564393613 1103 86-87 0.9635983582436793 1103 88-89 0.7401013000400063 1103 90-91 0.5044336726417704 1103 92-93 0.9200981134870005 1103 94-95 0.7271779012404203 1103 96-97 0.917595273612335 1103 98-99 0.9729931919181674 1103 100-101 1.3907762917084376 1104 1 0.24250338853101994 1104 2 0.22664857087145407 1104 3 0.3761384296123822 1104 4 0.2633521144709192 1104 5 0.284178211457764 1104 6 0.2581448688700263 1104 7 0.29204639890515693 1104 8 0.3876186346431538 1104 9 0.36423847789137653 1104 10-11 0.36833298637878187 1104 12-13 0.26449283187316297 1104 14-15 0.36069030704047833 1104 16-17 0.44250696767280573 1104 18-19 0.5040200760854887 1104 20-21 0.5561253435371611 1104 22-23 0.6000434450281489 1104 24-25 0.49097099304628955 1104 26-27 0.4491569112618592 1104 28-29 0.3753936686990258 1104 30-31 0.2944575802587579 1104 32-33 0.2342637566707495 1104 34-35 0.3988359417884695 1104 36-37 0.40328730191183126 1104 38-39 0.5849276668325913 1104 40-41 0.701304457486053 1104 42-43 0.5539047170385771 1104 44-45 0.641932703219176 1104 46-47 0.3956265802313155 1104 48-49 0.5678437845340767 1104 50-51 0.2025587381370002 1104 52-53 0.23397818139452653 1104 54-55 0.4985268169382735 1104 56-57 0.684316200336319 1104 58-59 0.5623611253325151 1104 60-61 0.59065113071469 1104 62-63 0.6700045624991233 1104 64-65 0.7113428487094922 1104 66-67 0.6940255336008789 1104 68-69 0.8846429530842101 1104 70-71 1.0651384927400365 1104 72-73 1.1525705175972583 1104 74-75 0.6476318850970451 1104 76-77 0.6470885055783064 1104 78-79 0.731540894716666 1104 80-81 1.0135735233641476 1104 82-83 1.162814880006966 1104 84-85 1.18462616750773 1104 86-87 1.0447462733481672 1104 88-89 1.2252690371377746 1104 90-91 1.571411144685591 1104 92-93 1.4672723383753379 1104 94-95 1.6236862310418765 1104 96-97 1.3496307771496454 1104 98-99 1.5019192832688688 1104 100-101 1.2433991196026746 1105 1 0.32056380188555167 1105 2 0.3330873626043669 1105 3 0.3007807189605529 1105 4 0.3701486168556585 1105 5 0.3107282909490152 1105 6 0.36104629971263336 1105 7 0.3799637279989554 1105 8 0.4445741195398156 1105 9 0.36558982924272954 1105 10-11 0.4201016668239319 1105 12-13 0.4774896522229213 1105 14-15 0.2945337092662328 1105 16-17 0.41484401059808107 1105 18-19 0.476436610266731 1105 20-21 0.5556881416293677 1105 22-23 0.7767723798453261 1105 24-25 0.5727277498030432 1105 26-27 0.5480042880504143 1105 28-29 0.5031361170297117 1105 30-31 0.7567389793048633 1105 32-33 0.728878224079331 1105 34-35 0.7792810928218543 1105 36-37 0.6060297502425129 1105 38-39 0.6695858544319506 1105 40-41 0.974337048900999 1105 42-43 0.6890795978016939 1105 44-45 0.6426083788948489 1105 46-47 0.8330868346033427 1105 48-49 0.7066553902574526 1105 50-51 0.5510484042737218 1105 52-53 0.49914113846924835 1105 54-55 0.6317740347443177 1105 56-57 0.7838591256145406 1105 58-59 0.952146498941417 1105 60-61 0.8344309399356717 1105 62-63 0.8053781714021397 1105 64-65 0.8048841841625958 1105 66-67 0.9834531965579529 1105 68-69 1.0407240341652937 1105 70-71 1.1651981111820042 1105 72-73 1.101298657501875 1105 74-75 1.0248576402639777 1105 76-77 0.8038651351490529 1105 78-79 1.051413708707127 1105 80-81 0.9482913294054853 1105 82-83 1.3168093156190501 1105 84-85 1.4033066126587599 1105 86-87 1.2263440475930025 1105 88-89 1.105734060985153 1105 90-91 1.349054228946322 1105 92-93 1.013933705624936 1105 94-95 1.2010709687207317 1105 96-97 1.3470274385168999 1105 98-99 1.8437118110908095 1105 100-101 2.0982480862163477 1106 1 0.012411879699353534 1106 2 -0.013253535959783846 1106 3 0.10972004272155189 1106 4 0.08750618980609914 1106 5 0.18393347853586306 1106 6 0.03877904635459117 1106 7 0.0634977715376337 1106 8 -0.043047464526885904 1106 9 -0.05291410776514738 1106 10-11 -0.13701389593661162 1106 12-13 -0.336544622904448 1106 14-15 -0.2347448919380284 1106 16-17 -0.07409762904989492 1106 18-19 -0.05298673387407149 1106 20-21 -0.11418216869948594 1106 22-23 -0.41817319681053533 1106 24-25 -0.32943760452766924 1106 26-27 -0.10359367396903707 1106 28-29 0.19588739076755957 1106 30-31 0.24916602886484185 1106 32-33 0.27220267984588986 1106 34-35 -0.03466054682613162 1106 36-37 0.19543456728744246 1106 38-39 -0.039492422548129014 1106 40-41 -0.08939037120089921 1106 42-43 0.10033480854740873 1106 44-45 -0.13711603055396893 1106 46-47 0.18984343488124722 1106 48-49 0.5740419117951987 1106 50-51 0.5495314982987409 1106 52-53 0.6153465576447914 1106 54-55 0.65709246920472 1106 56-57 0.6927116036136098 1106 58-59 0.5162502506783326 1106 60-61 0.37210463145952843 1106 62-63 0.5668070736717112 1106 64-65 0.46105023325939243 1106 66-67 0.947047453158234 1106 68-69 0.7245823018818243 1106 70-71 0.42666754147380814 1106 72-73 0.5137697089145625 1106 74-75 0.708495898716965 1106 76-77 0.3812447090258466 1106 78-79 0.1939797114861932 1106 80-81 0.21564311263846392 1106 82-83 0.7295524837524425 1106 84-85 0.6639249544836829 1106 86-87 0.25356729909832865 1106 88-89 0.49161294009585177 1106 90-91 0.9655375545600329 1106 92-93 0.7710550581029381 1106 94-95 0.5998088103140624 1106 96-97 0.5640434181370928 1106 98-99 0.7150816582422657 1106 100-101 0.8871318286897143 1107 1 0.18976038708026266 1107 2 0.054272019369658864 1107 3 0.34490421328870013 1107 4 0.2560069959925997 1107 5 0.3976245603911863 1107 6 0.38825072024823726 1107 7 0.4049608043642934 1107 8 0.23718609474739338 1107 9 0.49014251313934665 1107 10-11 0.3916941706246533 1107 12-13 0.34398479898938916 1107 14-15 0.6748620334388491 1107 16-17 0.5740622306893002 1107 18-19 0.6378884872401827 1107 20-21 0.6593076013416805 1107 22-23 0.7434210174315723 1107 24-25 0.6690001156082133 1107 26-27 0.7918588079896054 1107 28-29 0.8581296850334539 1107 30-31 0.8694334010099212 1107 32-33 0.8844316681209605 1107 34-35 1.014692155855414 1107 36-37 0.7878125826597753 1107 38-39 0.7357067759615958 1107 40-41 0.9835421126362363 1107 42-43 0.8982481161036446 1107 44-45 0.7429562914197021 1107 46-47 1.2043109604365725 1107 48-49 1.0268854803053955 1107 50-51 0.8386785060162154 1107 52-53 0.9605243100281271 1107 54-55 0.9625964531749105 1107 56-57 1.2599569504303574 1107 58-59 1.0713840150214082 1107 60-61 1.2533103107694927 1107 62-63 1.0172978299216098 1107 64-65 1.1296371370336047 1107 66-67 1.5181859763497982 1107 68-69 1.3869466981811982 1107 70-71 1.3109836918158528 1107 72-73 1.2902619366505128 1107 74-75 1.1233344266047602 1107 76-77 1.1252734499660342 1107 78-79 1.300086378570299 1107 80-81 1.4719274538353773 1107 82-83 1.2267394407506096 1107 84-85 1.4778680674593687 1107 86-87 1.2725228570889655 1107 88-89 1.26404449251363 1107 90-91 1.4062507556683421 1107 92-93 1.255955106630669 1107 94-95 1.2077924048100783 1107 96-97 1.6664447581286375 1107 98-99 1.8140062211013337 1107 100-101 2.3911611957941723 1108 1 0.3522081427902286 1108 2 0.4424544757862705 1108 3 0.3711165760756785 1108 4 0.24754620955610562 1108 5 0.39334331715752313 1108 6 0.32806726651519114 1108 7 0.2185872962381339 1108 8 0.18057198403214159 1108 9 0.2621244827080744 1108 10-11 0.3416872812677312 1108 12-13 0.12156847186960107 1108 14-15 0.24322085739281363 1108 16-17 0.18250562970117556 1108 18-19 0.38569209463869925 1108 20-21 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-0.6213811345458815 2316 74-75 -0.4205284192334702 2316 76-77 -0.35956872448317867 2316 78-79 -0.1027405225434741 2316 80-81 -0.15441758558440455 2316 82-83 -0.18077781252189595 2316 84-85 -0.1773446822290765 2316 86-87 0.3313190163038904 2316 88-89 0.144278635934608 2316 90-91 0.12851292731928865 2316 92-93 0.21865354773526846 2316 94-95 -0.16307893019137154 2316 96-97 -0.21887344720564172 2316 98-99 0.12782055278715987 2316 100-101 0.5243961559188612 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2833.0 3 552.0 4 527.0 5 542.0 6 668.0 7 788.0 8 940.0 9 1244.0 10 1396.0 11 1894.0 12 2421.0 13 3080.0 14 3734.0 15 4685.0 16 5984.0 17 7646.0 18 6671.0 19 4727.0 20 4655.0 21 4891.0 22 5258.0 23 5777.0 24 6495.0 25 7364.0 26 8725.0 27 10156.0 28 12203.0 29 14494.0 30 17505.0 31 21876.0 32 28163.0 33 36603.0 34 49785.0 35 69538.0 36 99375.0 37 129337.0 38 94067.0 39 9840.0 40 2.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.03567357770387 19.405654697537393 15.744626751383864 21.814044973374873 2 14.834934495265168 20.335024327826037 40.824443804985926 24.005597371922867 3 19.33914039177197 24.730094466936574 29.925629925629927 26.00513521566153 4 12.478090163359742 17.12385192456005 38.15934001729416 32.23871789478604 5 13.353880311418179 37.69182454244022 34.21115671696294 14.743138429178657 6 30.985929885926673 36.82229729137895 17.469978014534348 14.721794808160023 7 28.01716650487917 33.45366430432593 20.555575829677906 17.97359336111699 8 26.335349689955972 34.4680205061133 19.73363072647227 19.462999077458456 9 25.897176860121874 17.71185228224975 21.00726734425138 35.38370351337699 10-11 24.78083077727151 27.246621399675607 26.84361281393573 21.128935009117146 12-13 26.075499766056858 24.573373109750356 27.541932757222494 21.809194366970296 14-15 22.612409477878696 25.913652060740063 26.51468755862359 24.959250902757653 16-17 21.520641035091547 29.448334098451344 27.004895052326045 22.02612981413106 18-19 21.504479293940072 27.710604182815334 29.7861733823231 20.99874314092149 20-21 22.36534154392259 26.50542268163119 29.8725135247759 21.256722249670318 22-23 22.039943771594732 26.559601087783168 29.881266394477592 21.519188746144515 24-25 21.99510405703467 26.69865427256418 29.94232596703632 21.36391570336483 26-27 21.49275561173699 26.919113332418597 30.012538645008917 21.575592410835494 28-29 21.31968765100299 27.52776265213437 29.918336124075367 21.234213572787272 30-31 21.53597991343508 26.84797127154087 29.72891896034509 21.887129854678957 32-33 21.27501322968559 27.11308187806792 29.680772248681592 21.9311326435649 34-35 22.2107061761123 26.97075840705833 29.43056152646327 21.387973890366105 36-37 22.061962810053064 26.48092371309778 29.748965303833213 21.708148173015942 38-39 21.47482090511784 27.210162562766214 29.692716455420936 21.622300076695005 40-41 21.735557996052233 27.144363486440042 29.602194987576574 21.51788352993115 42-43 21.57478361139087 27.09338646348762 29.12386332121849 22.207966603903024 44-45 21.761875083015745 27.11290579190817 29.125937256882462 21.99928186819362 46-47 22.192948718416545 27.618139873990945 28.624256414485565 21.56465499310694 48-49 22.08119252293608 27.591472427432638 28.39694511617858 21.9303899334527 50-51 21.755422378803438 27.86646901815841 28.55918762667788 21.818920976360275 52-53 22.238996538958798 27.97143272213336 28.00260472368977 21.786966015218066 54-55 21.794194364852654 27.681935153329544 28.347538217439094 22.176332264378704 56-57 22.148732348322543 27.928908762937755 28.118706253903948 21.803652634835757 58-59 22.353824576356526 27.502856884325013 28.124949831213957 22.018368708104504 60-61 21.850547673736468 27.737639034413114 28.792928835978067 21.618884455872355 62-63 21.74676051528202 27.910574314414266 28.728417421050555 21.614247749253156 64-65 21.50661291205283 27.93242972070708 28.468686721272597 22.092270645967492 66-67 21.645241814908836 27.918879141069823 28.11326798735305 22.32261105666829 68-69 21.654103516465874 27.804744565495 28.468925358104507 22.07222655993462 70-71 21.846016208666512 27.85304492629041 28.170548563972858 22.130390301070214 72-73 21.854320099413112 27.659596203563375 28.461672612432622 22.024411084590895 74-75 21.606041520860046 28.46712025752946 27.908536465216955 22.018301756393537 76-77 21.863593176010077 27.896247797994956 27.985787489546404 22.254371536448566 78-79 21.929203617320635 27.935862032728696 27.967315091046025 22.167619258904644 80-81 22.11164405603438 28.17824052283575 27.81979796741471 21.890317453715156 82-83 22.05226156463677 28.126851319525713 28.115323891542953 21.705563224294565 84-85 21.812075885219336 28.33249066683009 27.898754531612397 21.956678916338173 86-87 22.02905191302083 28.28617936480952 27.732180732112877 21.95258799005677 88-89 21.796772621274783 28.30348100434578 27.50218164731154 22.397564727067895 90-91 21.930663086229668 28.062769536124225 27.882677446895453 22.12388993075065 92-93 22.28655528898073 27.966861052250614 27.914324407941205 21.832259250827452 94-95 21.88053857676864 27.867911077773748 27.939709639029815 22.311840706427795 96-97 21.85036138160511 27.933101533454707 28.02861865028257 22.187918434657607 98-99 21.883246152768372 28.14608971814212 27.405030591037526 22.565633538051983 100-101 22.026400217727737 27.31928561369817 28.121390509726606 22.532923658847487 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 953.0 1 560.0 2 179.5 3 181.5 4 158.5 5 162.5 6 241.0 7 493.0 8 818.0 9 987.0 10 915.5 11 796.0 12 835.0 13 931.5 14 968.5 15 1005.0 16 1044.0 17 1096.5 18 1170.0 19 1234.0 20 1396.0 21 1585.0 22 1865.5 23 2339.0 24 2764.0 25 3281.0 26 4076.0 27 5144.0 28 6154.0 29 7112.5 30 8435.0 31 9995.5 32 11905.5 33 13517.5 34 14658.0 35 15738.5 36 17397.0 37 19110.0 38 19822.0 39 20223.0 40 20794.5 41 22275.0 42 23546.0 43 24208.5 44 25285.0 45 26371.5 46 26330.5 47 27420.0 48 30514.0 49 29717.5 50 26158.5 51 23558.5 52 21688.5 53 20990.5 54 20522.5 55 18894.5 56 16780.5 57 15022.5 58 13366.0 59 11358.5 60 9143.0 61 7481.5 62 6117.0 63 4678.5 64 3461.0 65 2627.0 66 1881.5 67 1420.5 68 1070.0 69 737.5 70 551.0 71 412.0 72 348.5 73 270.0 74 181.0 75 144.5 76 99.5 77 68.0 78 53.5 79 34.5 80 23.5 81 17.5 82 9.0 83 4.5 84 4.5 85 5.0 86 5.0 87 3.0 88 1.5 89 2.5 90 2.5 91 1.5 92 1.0 93 1.0 94 1.0 95 1.5 96 1.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.30956775600525027 2 0.26717518330053125 3 0.255957904612341 4 0.26469864125248926 5 0.2667381464685239 6 0.21021471619556525 7 0.20191101638742442 8 0.20059990589140217 9 0.21254557929960477 10-11 0.21385668979562703 12-13 0.2105789135555714 14-15 0.21218138193959862 16-17 0.1983418822593639 18-19 0.203950521603459 20-21 0.1907665771712354 22-23 0.20074558483540464 24-25 0.20161965849941946 26-27 0.19797768489935771 28-29 0.2094863214755529 30-31 0.20482459526747382 32-33 0.20810237150752942 34-35 0.20489743473947503 36-37 0.19469990865930212 38-39 0.18348262997111187 40-41 0.18115176686707232 42-43 0.1938986744672885 44-45 0.19375299552328606 46-47 0.19397151393928977 48-49 0.20970483989155658 50-51 0.20198385585942563 52-53 0.22310730273978388 54-55 0.19637521651533052 56-57 0.18020485373105627 58-59 0.18268139577909828 60-61 0.17175547497891297 62-63 0.17831102745902414 64-65 0.17896658270703528 66-67 0.17721843537900564 68-69 0.1800591747870538 70-71 0.18239003789109332 72-73 0.17765547221101305 74-75 0.19164065083525023 76-77 0.1850122588831378 78-79 0.18836287459519463 80-81 0.18275423525109952 82-83 0.16301473833876473 84-85 0.16265054097875856 86-87 0.16760362507484255 88-89 0.17175547497891297 90-91 0.1800591747870538 92-93 0.1755431275229772 94-95 0.17343078283494137 96-97 0.17823818798702293 98-99 0.1854492957151452 100-101 0.17211967233891914 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 686441.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 64.87872503352826 #Duplication Level Percentage of deduplicated Percentage of total 1 84.2627217027178 54.66857951927342 2 9.504378112048812 12.332638682925985 3 2.636149962666483 5.130901457249536 4 1.0919324749342916 2.8337274718576753 5 0.5934560650686721 1.9251336432535011 6 0.38862687228096576 1.512816959241413 7 0.2585458965450832 1.1741889691346847 8 0.18966422408125472 0.984413843429217 9 0.13699771634350952 0.7999413451984669 >10 0.8497110490649659 10.225564603825218 >50 0.055842057999942275 2.4288420597114353 >100 0.028596345165937447 3.465017970661346 >500 0.0022516807215384148 0.9603705104792205 >1k 0.0011258403607692074 1.5578629637588086 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3292 0.47957508365613366 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2525 0.36783933360623855 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2046 0.2980591194290551 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1528 0.22259742643577526 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 1273 0.1854492957151452 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 948 0.13810363891434224 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 858 0.12499253395411988 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 691 0.10066415030570727 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 7.283947200123536E-5 16-17 0.0 0.0 0.0 0.0 1.4567894400247073E-4 18-19 0.0 0.0 0.0 0.0 1.4567894400247073E-4 20-21 0.0 0.0 0.0 0.0 1.4567894400247073E-4 22-23 0.0 0.0 0.0 0.0 1.4567894400247073E-4 24-25 0.0 0.0 0.0 0.0 1.4567894400247073E-4 26-27 0.0 0.0 0.0 2.9135788800494145E-4 1.4567894400247073E-4 28-29 0.0 0.0 0.0 0.0010925920800185303 1.4567894400247073E-4 30-31 0.0 0.0 0.0 0.0029135788800494144 1.4567894400247073E-4 32-33 0.0 0.0 0.0 0.004953084096084004 1.4567894400247073E-4 34-35 0.0 0.0 0.0 0.006409873536108711 1.4567894400247073E-4 36-37 0.0 0.0 0.0 0.009614810304163068 1.4567894400247073E-4 38-39 0.0 0.0 0.0 0.012309870768208775 1.4567894400247073E-4 40-41 0.0 0.0 0.0 0.015296289120259425 1.4567894400247073E-4 42-43 0.0 0.0 0.0 0.02338147051239655 1.4567894400247073E-4 44-45 0.0 0.0 0.0 0.036929612304626325 1.4567894400247073E-4 46-47 0.0 0.0 0.0 0.05178886459287834 1.4567894400247073E-4 48-49 0.0 0.0 0.0 0.08267280072140212 1.4567894400247073E-4 50-51 1.4567894400247073E-4 0.0 0.0 0.10700118436981473 1.4567894400247073E-4 52-53 1.4567894400247073E-4 0.0 0.0 0.1324221600982459 1.4567894400247073E-4 54-55 1.4567894400247073E-4 0.0 0.0 0.18086040897906738 1.4567894400247073E-4 56-57 1.4567894400247073E-4 0.0 0.0 0.23082828677191486 1.4567894400247073E-4 58-59 1.4567894400247073E-4 0.0 0.0 0.2836369039728105 1.4567894400247073E-4 60-61 1.4567894400247073E-4 0.0 0.0 0.34154428421379257 1.4567894400247073E-4 62-63 1.4567894400247073E-4 0.0 0.0 0.4154035088230452 1.4567894400247073E-4 64-65 1.4567894400247073E-4 0.0 0.0 0.46959607599196435 1.4567894400247073E-4 66-67 1.4567894400247073E-4 0.0 0.0 0.5503750504413343 1.4567894400247073E-4 68-69 1.4567894400247073E-4 0.0 0.0 0.6598527768591911 1.4567894400247073E-4 70-71 1.4567894400247073E-4 0.0 0.0 0.7487897721726995 1.4567894400247073E-4 72-73 1.4567894400247073E-4 0.0 0.0 0.878006995502891 1.4567894400247073E-4 74-75 1.4567894400247073E-4 0.0 0.0 1.0437896337777026 1.4567894400247073E-4 76-77 1.4567894400247073E-4 0.0 0.0 1.1812377174440338 1.4567894400247073E-4 78-79 1.4567894400247073E-4 0.0 0.0 1.3156993827583143 1.4567894400247073E-4 80-81 1.4567894400247073E-4 0.0 0.0 1.504062257353509 1.4567894400247073E-4 82-83 1.4567894400247073E-4 0.0 0.0 1.7072843842369556 1.4567894400247073E-4 84-85 1.4567894400247073E-4 0.0 0.0 1.9228892213606121 1.4567894400247073E-4 86-87 1.4567894400247073E-4 0.0 0.0 2.173165647156857 1.4567894400247073E-4 88-89 1.4567894400247073E-4 0.0 0.0 2.478071676954028 1.4567894400247073E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAACGTG 45 9.594336E-4 42.24724 5 CGTATAG 60 0.003939819 31.683117 1 GTATCAA 5930 0.0 26.286835 1 GAGTACT 2855 0.0 24.79186 12-13 TCAATAC 175 1.4789657E-7 24.437693 3 GGTCGTT 100 0.0016521838 23.751934 7 ATATCGT 100 0.0016521838 23.751934 6 GTACATG 2990 0.0 23.682865 1 GTACTTT 3005 0.0 23.474434 14-15 AGTACTT 2995 0.0 23.39506 12-13 GTGTAGC 390 0.0 23.153048 1 TATCAAC 6685 0.0 23.030285 2 TACATGG 3060 0.0 22.982435 2 CAACGCA 6740 0.0 22.917873 5 TCAACGC 6740 0.0 22.917873 4 ATCAACG 6780 0.0 22.707592 3 AACGCAG 6865 0.0 22.558283 6 AGAGTAC 4710 0.0 22.188643 10-11 ACATGGG 3085 0.0 21.717993 3 ATTAGCG 55 0.0028775053 21.591091 84-85 >>END_MODULE