FastQCFastQC Report
Thu 26 May 2016
SRR937610_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937610_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences686441
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47540.6925576997877458No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38030.5540170240413961No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23510.34249119734980865No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT21620.3149578769333417No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT12350.17991349584305133No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT10360.15092338598655966No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10280.1497579544345399No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT7550.10998760272186538No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA7270.1059085922897962No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC7200.10488883968177891No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC7050.10270365552174185No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6950.10124686608171715No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT6910.10066415030570727No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATACCG459.619528E-442.2247475
GTACTCG650.00583243129.241041
GTAAGGT3000.028.5017034
GTATCAA60600.024.93451
GGTATCA44150.023.785251
GTAGCAC1801.9475192E-723.7514213
TAAGGTG3650.023.4260585
GAGTACT37200.023.05245412-13
CTGGTCG1252.2514812E-422.8046889
GGTAAGG3550.022.7478393
AGGTAAG3600.022.4318982
AGTACTT38200.022.0758712-13
GTACATG26700.022.0676771
GTACTTT38650.022.05826214-15
TACATGG26950.021.8565942
AGGACCG2205.6274075E-821.59225
TCAACGC70600.021.3964634
AGAGTAC53450.021.37717210-11
CAACGCA70550.021.3442965
TATCAAC71100.021.1791842
Produced by FastQC (version 0.11.5)