##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937610_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 686441 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.18614127069916 33.0 31.0 34.0 31.0 34.0 2 32.401867021346334 34.0 31.0 34.0 31.0 34.0 3 32.48154466297905 34.0 31.0 34.0 31.0 34.0 4 36.11025419518939 37.0 35.0 37.0 35.0 37.0 5 36.03000840567507 37.0 35.0 37.0 35.0 37.0 6 36.07602255692769 37.0 35.0 37.0 35.0 37.0 7 36.02354754450856 37.0 35.0 37.0 35.0 37.0 8 35.9950643973772 37.0 35.0 37.0 35.0 37.0 9 37.67523355976697 39.0 37.0 39.0 35.0 39.0 10-11 37.59966115077625 39.0 37.0 39.0 35.0 39.0 12-13 37.501218604366585 39.0 37.0 39.0 35.0 39.0 14-15 38.68915755323473 40.0 38.0 41.0 34.5 41.0 16-17 38.6520349163293 40.0 38.0 41.0 34.5 41.0 18-19 38.65967796212639 40.0 38.0 41.0 34.0 41.0 20-21 38.561950699331774 40.0 38.0 41.0 34.0 41.0 22-23 38.404244501712455 40.0 38.0 41.0 34.0 41.0 24-25 38.47026619913437 40.0 38.0 41.0 34.0 41.0 26-27 38.34700651621917 40.0 38.0 41.0 34.0 41.0 28-29 38.20837479113281 40.0 38.0 41.0 34.0 41.0 30-31 38.24011604784679 40.0 38.0 41.0 34.0 41.0 32-33 38.1404243044923 40.0 37.0 41.0 34.0 41.0 34-35 37.99683148296795 40.0 37.0 41.0 33.0 41.0 36-37 37.82458871192134 40.0 37.0 41.0 33.0 41.0 38-39 37.66607181097866 40.0 37.0 41.0 33.0 41.0 40-41 37.49412331139894 40.0 37.0 41.0 33.0 41.0 42-43 37.273236884160475 40.0 36.5 41.0 32.5 41.0 44-45 36.934220129625125 40.0 36.0 41.0 31.0 41.0 46-47 36.55815212086691 39.0 36.0 41.0 30.5 41.0 48-49 36.184202283954484 39.0 35.5 41.0 30.0 41.0 50-51 35.92242669071341 39.0 35.0 40.0 29.0 41.0 52-53 35.66997746346736 39.0 35.0 40.0 28.0 41.0 54-55 35.44472139630354 39.0 35.0 40.0 28.0 41.0 56-57 35.17382484437847 38.0 35.0 40.0 27.0 41.0 58-59 34.892515161536096 38.0 34.0 40.0 26.5 41.0 60-61 34.511028624455705 38.0 34.0 40.0 26.0 41.0 62-63 34.16311380002068 37.0 34.0 40.0 26.0 41.0 64-65 33.76403725884672 37.0 33.0 39.5 24.5 41.0 66-67 33.307279868189696 36.0 33.0 39.0 23.0 40.0 68-69 32.85430940168201 36.0 32.0 39.0 22.0 40.0 70-71 32.402933391216436 35.0 32.0 38.0 21.5 40.0 72-73 31.889308767978605 35.0 31.0 37.5 20.0 39.5 74-75 31.38102546322262 35.0 31.0 37.0 18.5 39.0 76-77 30.167746390439966 33.5 29.5 35.5 18.0 38.0 78-79 30.785790038765168 34.0 31.0 36.0 18.0 38.0 80-81 30.562927476651307 34.0 30.5 35.5 16.5 37.0 82-83 30.181851754193005 34.0 30.0 35.0 11.5 37.0 84-85 29.77192723045389 34.0 30.0 35.0 7.0 36.5 86-87 29.342286518433486 34.0 29.0 35.0 4.5 36.0 88-89 28.936186066974436 34.0 29.0 35.0 2.0 36.0 90-91 28.3809045496991 33.5 29.0 35.0 2.0 35.0 92-93 27.855678783755632 33.0 27.0 35.0 2.0 35.0 94-95 27.323531811182605 33.0 26.5 35.0 2.0 35.0 96-97 26.708562716970576 33.0 25.5 34.5 2.0 35.0 98-99 25.733035322773553 32.0 24.0 34.0 2.0 35.0 100-101 22.98395637789701 29.5 10.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.33269395943989366 1101 2 0.2820208104940676 1101 3 0.29384944167420457 1101 4 0.14799205313835273 1101 5 0.12453050739818394 1101 6 0.16971979778094237 1101 7 0.16638677565053683 1101 8 0.03654794141768036 1101 9 0.1815814648854257 1101 10-11 0.17331909966729597 1101 12-13 0.33962425566826226 1101 14-15 0.45597238327747647 1101 16-17 0.389212879018487 1101 18-19 0.2544445848515764 1101 20-21 0.37317957077798525 1101 22-23 0.36056646099894607 1101 24-25 0.37222734364181775 1101 26-27 0.2917409069961039 1101 28-29 0.25142274163599154 1101 30-31 0.47802977068539576 1101 32-33 0.43707439677318405 1101 34-35 0.5668677227917911 1101 36-37 0.6912285050169373 1101 38-39 0.6632471077427624 1101 40-41 0.7220738370827391 1101 42-43 0.7203788494991059 1101 44-45 0.8748980131570079 1101 46-47 0.93528253913707 1101 48-49 1.0640148189521383 1101 50-51 1.0637749447494969 1101 52-53 1.0756009720565984 1101 54-55 0.9783440997440422 1101 56-57 1.015571441635565 1101 58-59 0.8455244953907481 1101 60-61 0.8718901748785441 1101 62-63 0.8764235673533847 1101 64-65 1.1035664090587858 1101 66-67 0.9291217836476449 1101 68-69 1.2174393436227788 1101 70-71 1.2500074550657558 1101 72-73 1.0083715282004277 1101 74-75 1.0612078116911192 1101 76-77 0.7837780197364488 1101 78-79 0.9434164297552847 1101 80-81 0.8442516751471949 1101 82-83 1.006841347583613 1101 84-85 0.5713009303400582 1101 86-87 0.5203496297895214 1101 88-89 0.6490263247529313 1101 90-91 0.8125931857388622 1101 92-93 0.9382450778996514 1101 94-95 1.0568348920782853 1101 96-97 1.3670003803229882 1101 98-99 1.1476887081721259 1101 100-101 1.529177609298479 1102 1 0.3303186799682578 1102 2 0.3542508442570593 1102 3 0.38780820408139505 1102 4 0.22295592221285432 1102 5 0.24914885346453275 1102 6 0.22026026258189546 1102 7 0.183757765913235 1102 8 0.19740857333257367 1102 9 0.3730429409362159 1102 10-11 0.2775511599438403 1102 12-13 0.3720686642163358 1102 14-15 0.4717494521018466 1102 16-17 0.24237263706060475 1102 18-19 0.26324511154930264 1102 20-21 0.49661823141895667 1102 22-23 0.36839166686261393 1102 24-25 0.22767121252051936 1102 26-27 0.31599910636048634 1102 28-29 0.3241875498086202 1102 30-31 0.30415649875934037 1102 32-33 0.24403735538507476 1102 34-35 0.23611525726149551 1102 36-37 0.4036208311877232 1102 38-39 0.3895521347445836 1102 40-41 0.42292874189411833 1102 42-43 0.3359959754966084 1102 44-45 0.27293843818640795 1102 46-47 0.34485426682553566 1102 48-49 0.4534645887054438 1102 50-51 0.5571655246432101 1102 52-53 0.6236217560579078 1102 54-55 0.6048629141357225 1102 56-57 0.33549136008552694 1102 58-59 0.34315533461944625 1102 60-61 0.2591506736505167 1102 62-63 0.3373972728505379 1102 64-65 0.41370680875676413 1102 66-67 0.4409278159519303 1102 68-69 0.38798220695426977 1102 70-71 0.35000598657767057 1102 72-73 0.22366473020197475 1102 74-75 0.4181342416619813 1102 76-77 0.28462056477314235 1102 78-79 0.5909229981801012 1102 80-81 0.5513384762539424 1102 82-83 0.46034228435663493 1102 84-85 0.40657572729098845 1102 86-87 0.42683630876701173 1102 88-89 0.6002303014186587 1102 90-91 0.8393802439534035 1102 92-93 0.8904482383307553 1102 94-95 0.7545301000345539 1102 96-97 1.1842788558365633 1102 98-99 1.246386403991952 1102 100-101 1.4430342628344164 1103 1 0.12791097596092982 1103 2 0.12699822764061963 1103 3 0.1517714484033732 1103 4 0.1081757879225691 1103 5 0.11661889500496159 1103 6 0.0955730933150889 1103 7 -0.008375776668721358 1103 8 0.03378582870600155 1103 9 0.14993898767020397 1103 10-11 0.07377446427014434 1103 12-13 0.12304800427628493 1103 14-15 0.25775305881662547 1103 16-17 0.1192070926174651 1103 18-19 0.05793536037496949 1103 20-21 0.08974059479883323 1103 22-23 0.20268047031279934 1103 24-25 0.21819652609088536 1103 26-27 0.14072312275073529 1103 28-29 0.1364944890364228 1103 30-31 0.34214534510817884 1103 32-33 0.36352222184114424 1103 34-35 0.3049198639587942 1103 36-37 0.20710866693023888 1103 38-39 0.1486546517796512 1103 40-41 0.031713822305569295 1103 42-43 0.3716025342845839 1103 44-45 0.661686348725901 1103 46-47 0.7711983463400145 1103 48-49 0.6970139887293811 1103 50-51 0.6484596520992909 1103 52-53 0.6174001058178007 1103 54-55 0.8031730566426347 1103 56-57 0.6563081021274186 1103 58-59 0.4751762917571014 1103 60-61 0.37452318596539413 1103 62-63 0.3457894069310328 1103 64-65 0.4825141050251247 1103 66-67 0.7466627883098624 1103 68-69 0.5990617604316881 1103 70-71 0.7735549293745336 1103 72-73 0.8305045783487941 1103 74-75 0.5921716778014741 1103 76-77 0.5694037786222808 1103 78-79 0.6787859053643501 1103 80-81 0.6752927374086077 1103 82-83 0.7824580892174957 1103 84-85 0.7743685173578605 1103 86-87 0.7913061337969687 1103 88-89 0.6041234555189767 1103 90-91 0.6392600201273027 1103 92-93 0.6465296946796855 1103 94-95 0.864113310656208 1103 96-97 0.9863439084916443 1103 98-99 1.0473621803702535 1103 100-101 1.5155477880148531 1104 1 0.31094328450590325 1104 2 0.3271123345098488 1104 3 0.19597807964257896 1104 4 0.1434054687493571 1104 5 0.2228735815370868 1104 6 0.20314163894153126 1104 7 0.2562983667147094 1104 8 0.20623227998236615 1104 9 0.25105071459616823 1104 10-11 0.31886379364777895 1104 12-13 0.20275654273029886 1104 14-15 0.2555377554553644 1104 16-17 0.25944606500360123 1104 18-19 0.26982937139442953 1104 20-21 0.22915310743209005 1104 22-23 0.2828429910965511 1104 24-25 0.3297050189021533 1104 26-27 0.27348728015301305 1104 28-29 0.09140108441346229 1104 30-31 0.4489371489747782 1104 32-33 0.3252141006922571 1104 34-35 0.22071632990603973 1104 36-37 0.41567075736808334 1104 38-39 0.4981167193961227 1104 40-41 0.40175832398456635 1104 42-43 0.5086668905588212 1104 44-45 0.4975550280203933 1104 46-47 0.5614870367191784 1104 48-49 0.47270515033731186 1104 50-51 0.3292383499769045 1104 52-53 0.3132229793547836 1104 54-55 0.1997090981123364 1104 56-57 0.4308761261224632 1104 58-59 0.5453895085927556 1104 60-61 0.5513521560693917 1104 62-63 0.5395387956442406 1104 64-65 0.7483545836219321 1104 66-67 0.6359163663752625 1104 68-69 0.5609777777759248 1104 70-71 0.4143872574806835 1104 72-73 0.5062169312324372 1104 74-75 0.37832888446531854 1104 76-77 0.3097177355224865 1104 78-79 0.38956411421252213 1104 80-81 0.6205956096415157 1104 82-83 0.5836896283561508 1104 84-85 0.5304993164319676 1104 86-87 0.4626538989324267 1104 88-89 0.6171560900835757 1104 90-91 0.879319681718929 1104 92-93 0.9266221212437671 1104 94-95 0.9212050525790687 1104 96-97 1.2222989829871231 1104 98-99 1.494546185847387 1104 100-101 1.6400106706803896 1105 1 0.14937730676345495 1105 2 0.12472759683099355 1105 3 0.09478571080315135 1105 4 0.06931961819292098 1105 5 1.3908233199799724E-4 1105 6 -0.1287741003271492 1105 7 0.020209336508031583 1105 8 0.04216232767711858 1105 9 0.22382495942923697 1105 10-11 0.18094646455397623 1105 12-13 0.22115876848546634 1105 14-15 0.2203231290642691 1105 16-17 0.1468467009336507 1105 18-19 0.19268310748346806 1105 20-21 0.26061177197899354 1105 22-23 0.1503708130361403 1105 24-25 0.07259055149356897 1105 26-27 0.07340778889705035 1105 28-29 0.09915148186973965 1105 30-31 0.13735129841833782 1105 32-33 0.11531743932500405 1105 34-35 -0.12769384497472203 1105 36-37 -0.07860587220265813 1105 38-39 0.11387586089055901 1105 40-41 0.16509298217216184 1105 42-43 0.02323366321383702 1105 44-45 -0.09503241236117077 1105 46-47 0.014130120979906735 1105 48-49 0.09607557959009227 1105 50-51 -0.05067023507874069 1105 52-53 0.12814746266162302 1105 54-55 -0.012771029073675777 1105 56-57 -0.09328921568994275 1105 58-59 0.15057631304426167 1105 60-61 0.2842416632236038 1105 62-63 0.3336166970750867 1105 64-65 0.4608943292499177 1105 66-67 0.13057057941182393 1105 68-69 0.1279094152957967 1105 70-71 0.19306372806892114 1105 72-73 0.07557305205914844 1105 74-75 0.01700138049075406 1105 76-77 -0.1217806746523955 1105 78-79 0.18153549736037533 1105 80-81 -0.029662736940363033 1105 82-83 0.22381283980289268 1105 84-85 0.2977489189756888 1105 86-87 0.2908732947988817 1105 88-89 0.17997405510742226 1105 90-91 0.2173165020686909 1105 92-93 0.6420434249003648 1105 94-95 0.5149053705440956 1105 96-97 0.5216034344974609 1105 98-99 0.6374317184388012 1105 100-101 0.6141561396787978 1106 1 -4.953194523835691E-4 1106 2 -0.03562441799068239 1106 3 0.10437348291986837 1106 4 0.012441433848316308 1106 5 -0.02169973193386454 1106 6 -0.010212266144655757 1106 7 -0.036923595404893206 1106 8 -0.061473614888882366 1106 9 0.032706386015618705 1106 10-11 -0.133886791583123 1106 12-13 -0.14897786884663589 1106 14-15 -0.1029746940019649 1106 16-17 -0.009834633017256067 1106 18-19 0.05498046737230311 1106 20-21 -0.009727505464262265 1106 22-23 -0.10655368904816243 1106 24-25 0.09550625320732564 1106 26-27 0.0361335878780622 1106 28-29 0.24135852216617337 1106 30-31 0.14966602745955981 1106 32-33 0.026549491200874797 1106 34-35 -0.22256415530357998 1106 36-37 -0.1930917452920582 1106 38-39 -0.21664173985052315 1106 40-41 -0.22010271027804862 1106 42-43 -0.11364104730029823 1106 44-45 -0.2946560807261491 1106 46-47 -0.19852041167410306 1106 48-49 -0.015191210590550952 1106 50-51 -0.09059033697776897 1106 52-53 -0.41825712279460703 1106 54-55 -0.20123791188979112 1106 56-57 -0.4646069553842409 1106 58-59 -0.5214651413327616 1106 60-61 -0.54173679902744 1106 62-63 -0.24301600324913863 1106 64-65 -0.027358334020121333 1106 66-67 -0.33433794305227593 1106 68-69 -0.2525676574230502 1106 70-71 -0.19466786062955066 1106 72-73 -0.1671550628088454 1106 74-75 -0.07400139857362831 1106 76-77 -0.3861218048052457 1106 78-79 -0.13729266382535954 1106 80-81 0.07011146407862867 1106 82-83 -0.024977109247590334 1106 84-85 -0.3096156016357092 1106 86-87 -0.03918691826271825 1106 88-89 -0.30075197546228694 1106 90-91 -0.20970989904293447 1106 92-93 -0.0029426797777283298 1106 94-95 0.3137300578993525 1106 96-97 0.16961756140806372 1106 98-99 0.07511119968011215 1106 100-101 0.42167698266165843 1107 1 0.09252138347962813 1107 2 0.006028029528927448 1107 3 0.006807696535808816 1107 4 0.11120945563882856 1107 5 0.1284106295194647 1107 6 0.050804311554500714 1107 7 -0.0657396753034547 1107 8 0.02563209729471083 1107 9 -0.054671707627250044 1107 10-11 0.060770461747281956 1107 12-13 0.03770361704118841 1107 14-15 0.009938378742667453 1107 16-17 0.21085383460222573 1107 18-19 0.09678789054976988 1107 20-21 0.3112067316328293 1107 22-23 0.20660984940614924 1107 24-25 0.12441782315769956 1107 26-27 0.23563735269075892 1107 28-29 0.2588316362376517 1107 30-31 0.20148268656006962 1107 32-33 0.013767912953817074 1107 34-35 -0.059014743115291424 1107 36-37 -0.1887070299619893 1107 38-39 0.1372897890860969 1107 40-41 0.1438396199922991 1107 42-43 0.0900314061678742 1107 44-45 -0.11668923612447202 1107 46-47 0.24150557414328233 1107 48-49 -0.12083764693311849 1107 50-51 -0.23683413403793452 1107 52-53 -0.24952620123733738 1107 54-55 -0.08572156117518404 1107 56-57 -0.2056708084860972 1107 58-59 -0.13133662111548716 1107 60-61 0.22747783443164593 1107 62-63 0.5397738716745408 1107 64-65 0.4767477382079335 1107 66-67 0.156574847918435 1107 68-69 0.12564652206693694 1107 70-71 -0.1139427624000291 1107 72-73 -0.08192314721578953 1107 74-75 -0.05104621628155215 1107 76-77 -0.17513262179307887 1107 78-79 0.20454450215513376 1107 80-81 0.230051039226165 1107 82-83 -0.24231246717874555 1107 84-85 0.05958963935927031 1107 86-87 0.1854119452491254 1107 88-89 0.4547387025170835 1107 90-91 0.4252629410816695 1107 92-93 0.2707505936525578 1107 94-95 0.47613358946586004 1107 96-97 1.0509406465194466 1107 98-99 0.6263862266834472 1107 100-101 0.7346025786917778 1108 1 0.16050621377180363 1108 2 0.07823177077780485 1108 3 -0.008165172520477881 1108 4 -0.16777997411925583 1108 5 -0.18762057598677018 1108 6 -0.012374372119033694 1108 7 0.06613245823196934 1108 8 0.10150032029991252 1108 9 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88-89 -0.22471810817360094 2316 90-91 -0.06274029970275308 2316 92-93 0.09195918498508604 2316 94-95 0.40997733550029025 2316 96-97 0.38753320247666423 2316 98-99 0.5278091132592486 2316 100-101 0.9642752789875502 >>END_MODULE >>Per sequence quality scores pass #Quality Count 4 5.0 5 6.0 6 27.0 7 70.0 8 172.0 9 327.0 10 541.0 11 920.0 12 1375.0 13 1960.0 14 2786.0 15 3998.0 16 5492.0 17 7780.0 18 7057.0 19 4393.0 20 3730.0 21 3795.0 22 3800.0 23 4110.0 24 4689.0 25 5724.0 26 6760.0 27 8581.0 28 10594.0 29 13122.0 30 16460.0 31 20896.0 32 26193.0 33 35125.0 34 48268.0 35 71024.0 36 106353.0 37 150066.0 38 103347.0 39 6895.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.194967710349005 19.752494579535124 16.33658172662206 21.71595598349381 2 15.423321159429578 20.645765622974153 40.10410217338416 23.826811044212103 3 19.53831429066737 24.614060057601456 30.60452391392705 25.243101737804125 4 12.879329760314434 17.74952253726103 37.7595452486084 31.611602453816133 5 13.373295954585988 37.67506717100777 34.12520326150941 14.826433612896833 6 31.063995303310843 37.1286097421337 16.96285623964769 14.844538714907763 7 27.943406643833917 33.74944095705239 20.276760857815894 18.030391541297796 8 26.59558505392306 34.23790245629267 19.514277264906962 19.652235224877302 9 26.131160580443186 17.455979465096053 20.83252602918532 35.58033392527544 10-11 24.912046337558508 27.18696872710109 26.7277158561333 21.1732690792071 12-13 26.326746462786417 24.426076897974404 27.58035943218107 21.666817207058113 14-15 22.796788070642634 25.734768173812462 26.864144187191613 24.604299568353287 16-17 21.49223312710051 28.94079753394684 27.62203889336447 21.944930445588188 18-19 21.262788790296618 26.978429318761556 30.698122635448637 21.06065925549319 20-21 22.020902014885475 25.772061983477094 31.029542233054265 21.17749376858317 22-23 21.725854455873932 25.744175306767907 31.13495748298081 21.39501275437735 24-25 21.9221316908518 25.885327362439014 31.066544684830887 21.125996261878296 26-27 21.45391452814645 26.271840148360408 31.078912616097774 21.195332707395366 28-29 21.12043879360894 26.874651729425175 30.961026474026752 21.043883002939133 30-31 21.356897388122213 26.190087713292183 30.860117621179388 21.592897277406216 32-33 21.278012239944875 26.320543207646395 30.73395965567325 21.66748489673548 34-35 21.928250206499904 26.114334662410897 30.710505345688848 21.246909785400348 36-37 21.94398353245217 25.645539820610946 30.869805270955553 21.54067137598133 38-39 21.357776406772054 26.414092449651356 30.815797773580588 21.412333369996002 40-41 21.48730111095248 26.433050035108778 30.736861887016744 21.342786966921995 42-43 21.451347470610475 26.177274724025867 30.328123827569804 22.043253977793853 44-45 21.68416513582376 26.29541358980597 30.161295726799537 21.85912554757073 46-47 22.08252420819852 26.874633071159792 29.721636673800077 21.321206046841606 48-49 22.09454272107872 26.976025616185513 29.295744281008858 21.633687381726908 50-51 21.783299657217444 27.114129255099854 29.55009971723717 21.55247137044553 52-53 22.283196953561923 27.43972169494538 28.73866799914341 21.53841335234929 54-55 21.65983675217535 27.18543909818906 29.186703591423008 21.96802055821258 56-57 22.177725398104133 27.2169785895656 28.957841970395126 21.64745404193514 58-59 22.509072156237753 26.82240716973491 28.904450637418222 21.76407003660912 60-61 21.90574280965152 26.93596390658484 29.80001194567341 21.358281338090237 62-63 21.713737961456264 27.167666267020763 29.699493474311705 21.419102297211268 64-65 21.40562699489104 27.232639076045867 29.32240352776131 22.039330401301786 66-67 21.573449138381886 27.18908107178913 29.12194930081391 22.11552048901508 68-69 21.708442714418492 26.936081627182645 29.440823497533646 21.91465216086522 70-71 22.00620447074437 27.15566753418541 29.01584332509518 21.822284669975037 72-73 21.848053947826543 26.937712053912865 29.298439341472903 21.915794656787693 74-75 21.699679943360024 27.644619129684855 28.8090309290966 21.84666999785852 76-77 21.727504621664497 27.07727248226723 29.103666593341597 22.091556302726673 78-79 21.917688483059724 27.154263804172533 28.89563706130607 22.03241065146167 80-81 22.030095834963117 27.603776292337063 28.5679530855187 21.798174787181118 82-83 22.087620324069185 27.366036471440903 28.825913880198566 21.72042932429135 84-85 21.78447158776788 27.629714825144568 28.848979187501868 21.73683439958569 86-87 21.796265083233664 27.80843510221563 28.501575517779386 21.893724296771317 88-89 21.415533163083207 27.779080794999132 28.386926188849444 22.418459853068217 90-91 21.6507905518419 27.379887405369807 28.785280524975377 22.184041517812915 92-93 21.970984385004304 27.009783318898613 29.040308206231284 21.978924089865803 94-95 21.646507128799126 26.975005863577493 29.000088864155842 22.378398143467535 96-97 21.60083677985435 26.934944153976815 29.237254184991862 22.22696488117697 98-99 21.596261230221916 27.2234273318872 28.503020705710274 22.677290732180605 100-101 21.339023700798187 26.8557319912692 29.074785882682068 22.730458425250543 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 198.0 1 142.0 2 131.0 3 185.0 4 141.0 5 110.0 6 280.5 7 703.0 8 1242.0 9 1532.5 10 1314.0 11 1012.0 12 982.5 13 1034.5 14 1111.0 15 1220.5 16 1269.5 17 1335.0 18 1426.0 19 1445.5 20 1547.0 21 1765.0 22 2052.0 23 2441.0 24 2820.0 25 3362.0 26 4122.5 27 5155.0 28 6152.5 29 7152.5 30 8470.0 31 9902.0 32 11600.5 33 13193.0 34 14376.0 35 15389.0 36 17190.5 37 19073.5 38 19727.0 39 19803.0 40 20608.0 41 22087.0 42 23212.0 43 24186.0 44 24866.0 45 26011.0 46 26544.5 47 28071.0 48 31270.5 49 29918.0 50 25872.5 51 23368.0 52 21765.5 53 21008.0 54 20284.5 55 18510.0 56 16385.0 57 14875.0 58 13615.5 59 11695.5 60 9238.0 61 7486.5 62 6272.0 63 4675.5 64 3253.0 65 2407.5 66 1827.5 67 1443.0 68 1023.0 69 672.0 70 475.5 71 366.0 72 296.5 73 208.5 74 164.0 75 144.5 76 115.0 77 75.5 78 41.0 79 29.0 80 20.5 81 15.5 82 12.0 83 9.0 84 3.5 85 2.5 86 0.5 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.022288878432378017 2 0.0 3 0.0 4 0.0 5 0.019375299552328605 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0036419736000617682 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.001456789440024707 24-25 0.0 26-27 6.555552480111182E-4 28-29 0.0019666657440333547 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 3.6419736000617675E-4 40-41 4.3703683200741215E-4 42-43 0.014495054928245836 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 4.3703683200741215E-4 70-71 7.283947200123535E-5 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 7.283947200123535E-5 82-83 0.0019666657440333547 84-85 3.6419736000617675E-4 86-87 0.0 88-89 0.0 90-91 0.012382710240210012 92-93 0.0025493815200432375 94-95 0.0 96-97 0.0 98-99 0.0017481473280296486 100-101 0.019666657440333545 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 686441.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 62.099676749017306 #Duplication Level Percentage of deduplicated Percentage of total 1 82.8824040607891 51.46970500356443 2 10.220988262449772 12.694401343072617 3 2.9233009958058074 5.446081406388631 4 1.232801349517332 3.062262612031144 5 0.6428616614986571 1.9960750686701394 6 0.42690539255498217 1.5906412128046041 7 0.2966960098875584 1.2897308414718431 8 0.1990176698288193 0.9887146370969866 9 0.14890988998771304 0.8322530429671838 >10 0.9214396924566122 10.633332352133895 >50 0.06836893009678428 2.905951184624481 >100 0.032298270535122006 3.705736218061589 >500 0.0023575379951591147 0.9886550521468933 >1k 0.0016502765966113805 2.3964600249655703 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4754 0.6925576997877458 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3803 0.5540170240413961 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2351 0.34249119734980865 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2162 0.3149578769333417 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 1235 0.17991349584305133 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 1036 0.15092338598655966 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1028 0.1497579544345399 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 755 0.10998760272186538 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 727 0.1059085922897962 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 720 0.10488883968177891 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 705 0.10270365552174185 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 695 0.10124686608171715 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 691 0.10066415030570727 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 1.4567894400247073E-4 0.0 10-11 0.0 0.0 0.0 1.4567894400247073E-4 0.0 12-13 0.0 0.0 0.0 1.4567894400247073E-4 0.0 14-15 0.0 0.0 0.0 1.4567894400247073E-4 0.0 16-17 0.0 0.0 0.0 1.4567894400247073E-4 0.0 18-19 0.0 0.0 0.0 1.4567894400247073E-4 0.0 20-21 0.0 0.0 0.0 1.4567894400247073E-4 0.0 22-23 0.0 0.0 0.0 1.4567894400247073E-4 0.0 24-25 0.0 0.0 0.0 1.4567894400247073E-4 0.0 26-27 0.0 0.0 0.0 4.370368320074122E-4 0.0 28-29 0.0 0.0 0.0 0.0011654315520197656 0.0 30-31 0.0 0.0 0.0 0.0028407394080481787 0.0 32-33 0.0 0.0 0.0 0.004516047264076593 0.0 34-35 0.0 0.0 0.0 0.0059728367041012986 0.0 36-37 0.0 0.0 0.0 0.009396291888159361 0.0 38-39 1.4567894400247073E-4 0.0 0.0 0.01223703129620754 0.0 40-41 1.4567894400247073E-4 0.0 0.0 0.015441968064261895 0.0 42-43 1.4567894400247073E-4 0.0 0.0 0.02352714945639902 0.0 44-45 1.4567894400247073E-4 0.0 0.0 0.037075291248628794 0.0 46-47 1.4567894400247073E-4 0.0 0.0 0.05178886459287834 0.0 48-49 1.4567894400247073E-4 0.0 0.0 0.08259996124940089 0.0 50-51 1.4567894400247073E-4 0.0 0.0 0.10714686331381722 0.0 52-53 1.4567894400247073E-4 0.0 0.0 0.13234932062624463 0.0 54-55 1.4567894400247073E-4 0.0 0.0 0.18115176686707232 0.0 56-57 1.4567894400247073E-4 0.0 0.0 0.2321393972679371 0.0 58-59 1.4567894400247073E-4 0.0 0.0 0.2858949276048488 0.0 60-61 1.4567894400247073E-4 0.0 0.0 0.3439479867898334 0.0 62-63 1.4567894400247073E-4 0.0 0.0 0.41795289034308847 0.0 64-65 1.4567894400247073E-4 0.0 0.0 0.4734565680080298 0.0 66-67 1.4567894400247073E-4 0.0 0.0 0.5543812214014023 0.0 68-69 1.4567894400247073E-4 0.0 0.0 0.6642959846512664 0.0 70-71 1.4567894400247073E-4 0.0 0.0 0.753888535212786 0.0 72-73 1.4567894400247073E-4 0.0 0.0 0.8847810663990059 0.0 74-75 1.4567894400247073E-4 0.0 0.0 1.0521661730578447 0.0 76-77 1.4567894400247073E-4 0.0 0.0 1.190634009332193 0.0 78-79 1.4567894400247073E-4 0.0 0.0 1.3264067851424959 0.0 80-81 1.4567894400247073E-4 0.0 0.0 1.5170276833697287 0.0 82-83 1.4567894400247073E-4 0.0 0.0 1.7220707970532063 0.0 84-85 1.4567894400247073E-4 0.0 0.0 1.9396422999208962 0.0 86-87 1.4567894400247073E-4 0.0 0.0 2.1943619335092164 0.0 88-89 1.4567894400247073E-4 0.0 0.0 2.5013803079944235 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CATACCG 45 9.619528E-4 42.224747 5 GTACTCG 65 0.005832431 29.24104 1 GTAAGGT 300 0.0 28.501703 4 GTATCAA 6060 0.0 24.9345 1 GGTATCA 4415 0.0 23.78525 1 GTAGCAC 180 1.9475192E-7 23.751421 3 TAAGGTG 365 0.0 23.426058 5 GAGTACT 3720 0.0 23.052454 12-13 CTGGTCG 125 2.2514812E-4 22.804688 9 GGTAAGG 355 0.0 22.747839 3 AGGTAAG 360 0.0 22.431898 2 AGTACTT 3820 0.0 22.07587 12-13 GTACATG 2670 0.0 22.067677 1 GTACTTT 3865 0.0 22.058262 14-15 TACATGG 2695 0.0 21.856594 2 AGGACCG 220 5.6274075E-8 21.5922 5 TCAACGC 7060 0.0 21.396463 4 AGAGTAC 5345 0.0 21.377172 10-11 CAACGCA 7055 0.0 21.344296 5 TATCAAC 7110 0.0 21.179184 2 >>END_MODULE