FastQCFastQC Report
Thu 26 May 2016
SRR937609_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937609_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences629446
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31700.503617466788255No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23860.37906349392958255No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT19350.30741318556317776No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14500.2303613018432082No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT11900.1890551373747708No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT7960.1264604112187543No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN7800.12391849340531197No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT6590.10469523994115461No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC6480.10294767144441302No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTAAC700.008347549527.1702481
GTATAGG3300.024.4943941
GAGTACT25750.023.79739212-13
GGTATCA39800.023.1766341
GTACTTT26600.022.85836814-15
GTACATG26200.022.6850831
CTAACAC2354.618414E-922.2423133
GTATCAA51450.022.1797961
GACGTAT1553.8987913E-521.454478
TAAGGTG3151.4551915E-1121.1189635
ACACGGT1353.789894E-421.1139228
TACATGG27000.020.9479712
AGAGTAC40800.020.84140610-11
AGTACTT26850.020.78789312-13
GTAAGGT2751.2478267E-920.7366314
CCACTCG1404.8336334E-420.3663354
CGCGATC704.8503326E-420.35823422-23
ACATGGG26700.020.2884433
GTGTAAG2351.11785994E-720.2331661
GTATACC1200.004733436319.811641
Produced by FastQC (version 0.11.5)